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Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method.

Abstract：

:Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000 instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity.

journal_name

Int J Antimicrob Agents

journal_title

International journal of antimicrobial agents

authors

Viveiros M,Martins A,Paixão L,Rodrigues L,Martins M,Couto I,Fähnrich E,Kern WV,Amaral L

doi

10.1016/j.ijantimicag.2007.12.015

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

458-62

issue

5

eissn

0924-8579

issn

1872-7913

pii

S0924-8579(08)00032-0

journal_volume

31

pub_type

杂志文章

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