Effect of psychotropic drugs on the 21-hydroxylation of neurosteroids, progesterone and allopregnanolone, catalyzed by rat CYP2D4 and human CYP2D6 in the brain.

abstract：:Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin and peroxidase-biotin, or avi...

journal_title：Biological & pharmaceutical bulletin

authors： Muratsugu M,Muramoto E,Fukui T

更新日期：2003-11-01 00:00:00

abstract：:The blood clearance, tissue uptake and antitumor efficacy against liver metastasis of M5076 reticulosarcoma in mice and against primary liver cancer in rats of doxorubicin (DOX) encapsulated in two types of liposomes, with and without a soYbean-derived sterylglucoside mixture (SG), were examined. Liposomes entrapping ...

journal_title：Biological & pharmaceutical bulletin

authors： Shimizu K,Qi XR,Maitani Y,Yoshii M,Kawano K,Takayama K,Nagai T

更新日期：1998-07-01 00:00:00

abstract：:To develop a piroxicam-loaded gelatin microcapsule with enhanced bioavailability, a gelatin microcapsule encapsulated ethanol and piroxicam has been formulated by using gelatin as a water-soluble polymer shell. The aqueous solubility and bioavailability of piroxicam in piroxicam-loaded microcapsule in rats were then e...

journal_title：Biological & pharmaceutical bulletin

authors： Piao MG,Yang CW,Li DX,Kim JO,Jang KY,Yoo BK,Kim JA,Woo JS,Lyoo WS,Han SS,Lee YB,Kim DD,Yong CS,Choi HG

更新日期：2008-06-01 00:00:00

abstract：:Hydroxyproline (Hyp) content in biological fluids is used as a parameter of collagen catabolism, especially bone resorption. In this paper, we examined the relationship between aging and serum free 4-Hyp and proline (Pro) content using a newly introduced analytical technique which is chemiluminescence determination wi...

journal_title：Biological & pharmaceutical bulletin

authors： Koike K,Li Y,Seo M,Sakurada I,Tezuka K,Uchikura K

更新日期：2000-01-01 00:00:00

abstract：:A convenient and sensitive high performance liquid chromatographic method with post-column platinum catalyst reduction and electrochemical detection was applied to the simultaneous determination of oxidized and reduced coenzyme Q (CoQ and CoQH2) and alpha-tocopherol (alpha-TP) in human and rat samples. After the separ...

journal_title：Biological & pharmaceutical bulletin

authors： Wakabayashi H,Yamato S,Nakajima M,Shimada K

更新日期：1994-08-01 00:00:00

abstract：:We studied in vitro metabolites of 2,4,6,2',4',6'-hexachlorobiphenyl (HCB, IUPAC PCB No. 155) produced by liver microsomes of a phenobarbital (PB)-treated beagle dog. The major metabolites were 3-hydroxy-2,4,6,2',4',6'-HCB (M-1), 4-hydroxy-2,6,2',4',6'-pentachlorobiphenyl (PenCB, M-2) and 3,4-dihydroxy-2,6,2',4',6'-Pe...

journal_title：Biological & pharmaceutical bulletin

authors： Ariyoshi N,Yoshimura H,Oguri K

更新日期：1993-09-01 00:00:00

abstract：:Defining a quantitative and reliable relationship between in vitro drug release and in vivo absorption is highly desired for rational development, optimization, and evaluation of controlled-release dosage forms and manufacturing process. During the development of once daily extended-release (ER) tablet of glipizide, a...

journal_title：Biological & pharmaceutical bulletin

authors： Ghosh A,Bhaumik UK,Bose A,Mandal U,Gowda V,Chatterjee B,Chakrabarty US,Pal TK

更新日期：2008-10-01 00:00:00

abstract：:The tumor suppressor phosphatase and tensin homolog (PTEN) functions as a phosphoinositide 3-phosphatase, that antagonizes phosphatidylinositol 3-kinase action, and negatively regulates cell proliferation and survival signals. Inactivation of PTEN by loss-of-function mutations gives rise to deregulated hyperproliferat...

journal_title：Biological & pharmaceutical bulletin

authors： Maehama T

更新日期：2007-09-01 00:00:00

abstract：:The effects of conjugated linoleic acid (CLA) against anaphylaxis and allergic pruritus were investigated using a in vivo assay. Inhibitory effects of CLA were observed on the immediate (type 1) hypersensitivity reaction, with CLA significantly suppressing the decrease in blood pressure (BP) and blood flow (BF) induce...

journal_title：Biological & pharmaceutical bulletin

authors： Ishiguro K,Oku H,Suitani A,Yamamoto Y

更新日期：2002-12-01 00:00:00

abstract：:Highly inhibitory effects of hybrid liposomes on the growth of human breast tumor cells in vitro were obtained. It is worthy to note that induction of apoptosis through activation of caspases by hybrid liposomes was clearly observed. ...

journal_title：Biological & pharmaceutical bulletin

authors： Nagami H,Matsumoto Y,Ueoka R

更新日期：2006-02-01 00:00:00

abstract：:In brain ischemia, cell destructive necrosis occurs in the core, which in turn links to cell death expansion in the vicinity. Apoptosis, on the other hand, occurs in the surroundings of the core, called the penumbra, several days later. As cells showing apoptosis disappear by microglial phagocytosis in the brain, cell...

journal_title：Biological & pharmaceutical bulletin

authors： Ueda H,Fujita R

更新日期：2004-07-01 00:00:00

abstract：:We used three human urological cancer cell lines, PC-3, LNCaP and SKRC-1, to investigate the effects of the extract from Serenoa repens (Palmae) on tumor cell invasion. The invasion activity of these cell lines was determined in vitro using a Transwell cell-culture chamber. The invasion activity of PC-3 cells into Mat...

journal_title：Biological & pharmaceutical bulletin

authors： Ishii K,Usui S,Sugimura Y,Yamamoto H,Yoshikawa K,Hiran K

更新日期：2001-02-01 00:00:00

abstract：:The gastric mucus is an important factor in the gastric mucosal protection from acid, pepsin and various reagents (alcohol, aspirin, etc.). MUC5AC is the mucin secreted from surface mucous cells, and belongs to the gel-forming mucin. We examined the regulation of rat MUC5AC (rMUC5AC) mRNA by glucocorticoid in vivo and...

journal_title：Biological & pharmaceutical bulletin

authors： Tanaka T,Kobayashi T,Sunaga K,Tani S

更新日期：2001-06-01 00:00:00

abstract：:We investigated the cytotoxicity of eight vitamin K3 (VK3) analogs against neuroblastoma cell lines (IMR-32, LA-N-1, NB-39, and SK-N-SH) and normal cell lines (human umbilical vein endothelial cells (HUVEC) and human dermal fibroblasts (HDF)) using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) a...

journal_title：Biological & pharmaceutical bulletin

authors： Kitano T,Yoda H,Tabata K,Miura M,Toriyama M,Motohashi S,Suzuki T

更新日期：2012-01-01 00:00:00

abstract：:DNA rich in non-methylated CG motifs (CpGs) enhances induction of immune responses against co-administered antigen encoding genes. CpGs are therefore among the promising adjuvants known to date. However, naked plasmid DNA, even which contains CpG motifs, are taken up by antigen presenting cells via the endocytosis pat...

journal_title：Biological & pharmaceutical bulletin

authors： Yoshikawa T,Imazu S,Gao JQ,Hayashi K,Tsuda Y,Okada N,Tsutsumi Y,Akashi M,Mayumi T,Nakagawa S

更新日期：2006-01-01 00:00:00

abstract：:Telekin, a eudesmane-type sesquiterpene lactone compound isolated from Chinese folk medicine Carpesium divaricatum, has been reported to strongly inhibit the proliferation of cancer cells. In this study, the involvement of a mitochondria-mediated pathway in the pro-apoptotic action of telekin was investigated in human...

journal_title：Biological & pharmaceutical bulletin

authors： Zheng B,Wu L,Ma L,Liu S,Li L,Xie W,Li X

更新日期：2013-01-01 00:00:00

abstract：:A high performance liquid chromatography (HPLC) method was developed for the first time to quantify simultaneously the six major active ingredients in Acanthopanax senticosus (Rupr. et Maxim.) Harms, namely protocatechuic acid, syringin, chlorogenic acid, caffeic acid, liriodendrin and isofraxidin. The analysis was pe...

journal_title：Biological & pharmaceutical bulletin

authors： Li Q,Jia Y,Xu L,Wang X,Shen Z,Liu Y,Bi K

更新日期：2006-03-01 00:00:00

abstract：:Mucus on the nasal mucosa is translocated to the pharynx by ciliary beating, which is an important nonspecific defense mechanism called mucociliary clearance (MC). MC is one of the important factors determining the rate and extent of drug absorption after nasal application. The purpose of this study is to evaluate MC ...

journal_title：Biological & pharmaceutical bulletin

authors： Inoue D,Furubayashi T,Ogawara K,Kimura T,Higaki K,Katsumi H,Sakane T,Yamamoto A,Higashi Y

更新日期：2012-01-01 00:00:00

abstract：:Here, we confirmed that stable expression of B-cell lymphoma-xL (Bcl-xL) in N18TG neuroglioma cells could suppress c-Jun N-terminal protein kinase (JNK) activation, nuclear fragmentation, and cell death caused by etoposide treatment. Moreover, additional overexpression of JNK1 led to partially antagonize the antiapopt...

journal_title：Biological & pharmaceutical bulletin

authors： Jeong HS,Choi HY,Choi TW,Kim BW,Kim JH,Lee ER,Cho SG

更新日期：2008-09-01 00:00:00

abstract：:This study sought to investigate the ameliorating effects of soy 11S protein on the impacts of alcohol consumption in rat hepatocytes and in reducing total cholesterol levels and total lipid levels in the serum. Liver histology and the clinically important enzyme markers (Aspartate Aminotransferase: AST and Alanine Am...

journal_title：Biological & pharmaceutical bulletin

authors： Park KJ,Kim HY,Chang BJ,Lee HH

更新日期：2004-10-01 00:00:00

abstract：:Two conjugates of marine polysaccharide (MPS) and bovine serum albumin (BSA) were prepared using two methods, periodate oxidation and reductive amination, with the intent of enhancing its immunogenicity. Sera samples from Balb/c mice immunized with the products named MPS-BSAp and MPS-BSAr respectively were evaluated b...

journal_title：Biological & pharmaceutical bulletin

authors： Gan L,Xin X,Geng M,Ding J

更新日期：2004-10-01 00:00:00

abstract：:The effects of the angiotensin II type 1 receptor antagonist YM358 on blood pressure were compared to those of the angiotensin converting enzyme inhibitor enalapril in one-kidney, one-clip renal hypertensive rats (1K1C RHR), two-kidney, one-clip renal hypertensive rats (2K1C RHR) and normotensive rats (NTR). Additiona...

journal_title：Biological & pharmaceutical bulletin

authors： Tokioka T,Shibasaki M,Fujimori A,Matsuda-Satoh Y,Uchida W,Inagaki O,Yanagisawa I

更新日期：2000-02-01 00:00:00

abstract：:The pulmonary vein contains a myocardial layer extending from the left atrium, which is receiving attention as the source of ectopic electrical activity underlying atrial fibrillation. Electrophysiological and pharmacological analysis of the pulmonary vein myocardium performed in various experimental animal species ha...

journal_title：Biological & pharmaceutical bulletin

authors： Namekata I,Tsuneoka Y,Tanaka H

更新日期：2013-01-01 00:00:00

abstract：:Dermatan sulfate-proteoglycans (DS-PGs) were extracted from rabbit, rat and bovine defatted livers by magnesium chloride extraction and DEAE-cellulose chromatography, and then submitted successively to Asahipak GS-520 gel filtration chromatography, Asahipak ES-502N anion exchange chromatography, and cellulose acetate ...

journal_title：Biological & pharmaceutical bulletin

authors： Koshiishi I,Motoki K,Qiu G,Imanari T

更新日期：1993-04-01 00:00:00

abstract：:Plasma hyaluronan-binding protein (PHBP), a serine protease that can activate coagulation factor VII and prourokinase, circulates in a single-chain form (pro-PHBP) and autoproteolytically converts to an active two-chain form with the aid of an effector such as spermidine and heparin. It has been postulated that PHBP p...

journal_title：Biological & pharmaceutical bulletin

authors： Yamamoto E,Nishimura N,Okada K,Sekido C,Yamamichi S,Hasumi K

更新日期：2011-01-01 00:00:00

abstract：:DY-9760e (3-[2-[4-(3-chloro-2-methylphenyl)-1-piperazinyl]ethyl]-5,6-dimethoxy-1-(4-imidazolylmethyl)-1H-indazole dihydrochloride 3.5 hydrate), a calmodulin antagonist, provides protection against Ca(2+) overload-associated cytotoxicity and brain injury after cerebral ischemia in rats. In this study, we assessed the e...

journal_title：Biological & pharmaceutical bulletin

authors： Sugimura M,Takamori H,Fukushi H,Kitano Y,Kanazawa Y,Shirasaki Y

更新日期：2005-04-01 00:00:00

abstract：:We investigated acute cytotoxic effects and Hg accumulation after exposure to methylmercury (MeHg) or Hg(2+) in the presence or absence of serum in cultured astrocytes prepared from the cerebral hemisphere or cerebellum of newborn rats. Dose-related changes in viable cell numbers after exposure to mercuric compounds w...

journal_title：Biological & pharmaceutical bulletin

authors： Adachi T,Kunimoto M

更新日期：2005-12-01 00:00:00

abstract：:Information on the comparative digestibility of food allergens and non-allergenic proteins is crucial when stability to digestion is to be used as a criterion to assess the allergenic potential of novel proteins. Preheating effect on in vitro digestibility has not been fully examined. In this study we investigated the...

journal_title：Biological & pharmaceutical bulletin

authors： Takagi K,Teshima R,Okunuki H,Sawada J

更新日期：2003-07-01 00:00:00

abstract：:Our previous study using apoptosis analysis suggested that Ca(2+) release through inositol 1,4,5-trisphosphate (IP3) receptors and the subsequent Ca(2+) influx through store-operated channels (SOCs) constitute a triggering signal for H2O2-induced β-cell apoptosis. In the present study, we further examined the obligato...

journal_title：Biological & pharmaceutical bulletin

authors： Sato T,Kaneko YK,Sawatani T,Noguchi A,Ishikawa T

更新日期：2015-01-01 00:00:00

abstract：:Four lavandulyl flavanones, (2S)-2'-methoxykurarinone (1), sophoraflavanone G (2), leachianone A (3), and (-)-kurarinone (4), which are isolated from the roots of Sophora flavescens have been tested for in vitro antimalarial activity against Plasmodium falciparum. Compounds 1-3 showed moderate antimalarial activities ...

journal_title：Biological & pharmaceutical bulletin

authors： Kim YC,Kim HS,Wataya Y,Sohn DH,Kang TH,Kim MS,Kim YM,Lee GM,Chang JD,Park H

更新日期：2004-05-01 00:00:00