Prostaglandin E2-regulated cervical ripening: analysis of proteoglycan expression in the rat cervix.

Abstract:

OBJECTIVE:Prostaglandins reduce cervical resistance by reorganizing collagen fibrils. Proteoglycans are involved in collagen fibril organization and structure. We evaluated the changes in proteoglycan composition induced by prostaglandin E(2) (PGE(2)). STUDY DESIGN:Prostaglandins were administered intravaginally to induce cervical ripening in timed pregnant Sprague-Dawley rats. Changes in proteoglycan messenger ribonucleic acid (mRNA) expression were measured using reverse transcription (RT-PCR) for core protein. Fluorophore assisted carbohydrate gel electrophoresis (FACE) was used to evaluate proteoglycan glycosaminoglycan composition along with size exclusion high-performance liquid chromatography (HPLC). RESULTS:No change in core protein mRNA expression was detected after PGE(2) treatment. Total glycosaminoglycan (GAG) decreased more than 20% after PGE(2) (P = .02). FACE demonstrated a shift in disaccharide subunit composition after PGE(2), with a decrease in 4-sulfated disaccharides (P = .02). HPLC confirmed a decrease in total GAG (P = .04). CONCLUSION:Although there was no change in core protein mRNA expression, alterations in GAG composition was detected after PGE(2). The decrease in sulfated GAG could decrease electrostatic interactions that would weaken interfibrillar interactions. These findings would be consistent with a decline in cervical resistance.

journal_name

Am J Obstet Gynecol

authors

Ji H,Dailey TL,Long V,Chien EK

doi

10.1016/j.ajog.2007.11.024

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

536.e1-7

issue

5

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(07)02135-7

journal_volume

198

pub_type

杂志文章
  • Herceptin (trastuzumab) therapy in a twin pregnancy with associated oligohydramnios.

    abstract::Herceptin (trastuzumab) is an adjuvant chemotherapy agent used in treatment of certain breast cancers. Limited information is available on the use of herceptin in pregnancy. This case is a twin pregnancy exposed to herceptin until 23 weeks' gestation. One twin had chronic renal failure develop, whereas the other twin ...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2009.02.017

    authors: Beale JM,Tuohy J,McDowell SJ

    更新日期:2009-07-01 00:00:00

  • Endocrine impact of pure estradiol replacement in postmenopausal women: alterations in anterior pituitary hormone release and circulating sex steroid hormone concentrations.

    abstract::Thirteen healthy postmenopausal volunteers were studied under basal conditions and at intervals (days 1, 5, 10, and 30) after intravaginal placement of a polysiloxane ring impregnated with 400 mg of estradiol. Mean serum estradiol concentrations rose 26-fold with a twofold increase in serum estrone concentrations. Ser...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(86)90821-5

    authors: Veldhuis JD,Samojlik E,Evans WS,Rogol AD,Ridgeway CE,Crowley WF,Kolp L,Checinska E,Kirschner MA,Thorner MO

    更新日期:1986-08-01 00:00:00

  • Cardiovascular risk factors in women who had hypertensive disorders late in pregnancy: a cohort study.

    abstract:OBJECTIVE:The purpose of this study was to determine cardiovascular risk factors in women with a history of hypertensive pregnancy disorders at term (HTP) 2.5 years after pregnancy. STUDY DESIGN:In a multicenter cohort study in The Netherlands from June 2008 through November 2010, cardiovascular risk factors were comp...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1016/j.ajog.2013.02.016

    authors: Hermes W,Franx A,van Pampus MG,Bloemenkamp KW,Bots ML,van der Post JA,Porath M,Ponjee GA,Tamsma JT,Mol BW,de Groot CJ

    更新日期:2013-06-01 00:00:00

  • External funding of obstetrical publications: citation significance and trends over 2 decades.

    abstract:OBJECTIVE:The objective of the study was to identify the external funding status of the most frequently cited obstetrical publications (citation classics) and to assess trends in funded vs nonfunded manuscripts as well as each publication's type of external funding. STUDY DESIGN:For the first objective, the citation c...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2013.05.021

    authors: Vintzileos WS,Ananth CV,Vintzileos AM

    更新日期:2013-08-01 00:00:00

  • Use of laparoscopy to determine the microbiologic etiology of acute salpingitis.

    abstract::To determine the microbiologic etiology of acute salpingitis, laparoscopy was used in 26 patients to obtain specimens for a variety of microorganisms directly from the fallopian tube. Simultaneous culdocentesis was performed to obtain peritoneal fluid for microbiologic analysis. A variety of microorganisms were isolat...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(79)90798-1

    authors: Sweet RL,Mills J,Hadley KW,Blumenstock E,Schachter J,Robbie MO,Draper DL

    更新日期:1979-05-01 00:00:00

  • Efficacy of ultrasound-indicated cerclage in twin pregnancies.

    abstract:OBJECTIVE:We sought to compare the perinatal outcomes in twin pregnancies with short cervical length (CL) with ultrasound-indicated cerclage (UIC) vs no cerclage (control). STUDY DESIGN:This was a retrospective cohort study of asymptomatic twin pregnancies with transvaginal ultrasound (TVU) CL ≤25 mm at 16-24 weeks fr...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2015.01.031

    authors: Roman A,Rochelson B,Fox NS,Hoffman M,Berghella V,Patel V,Calluzzo I,Saccone G,Fleischer A

    更新日期:2015-06-01 00:00:00

  • Correlation of amniotic fluid index and nonstress test in patients with preterm premature rupture of membranes.

    abstract::The amniotic fluid index and the nonstress test are commonly used in the expectant management of preterm premature rupture of membranes. This study was designed to investigate the interrelationship of the nonstress test and the amniotic fluid index during the preterm rupture of membranes latency period. Fifty patients...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(91)90477-9

    authors: Harding JA,Jackson DM,Lewis DF,Major CA,Nageotte MP,Asrat T

    更新日期:1991-10-01 00:00:00

  • Is cerclage height associated with the incidence of preterm birth in women with an ultrasound-indicated cerclage?

    abstract:OBJECTIVE:Our aim was to determine whether there was a cerclage height threshold associated with spontaneous preterm birth in patients with an ultrasound-indicated cerclage. STUDY DESIGN:We performed a retrospective cohort study of women with an ultrasound-indicated cerclage. Functional cervical length and the cerclag...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2008.09.021

    authors: Scheib S,Visintine JF,Miroshnichenko G,Harvey C,Rychlak K,Berghella V

    更新日期:2009-05-01 00:00:00

  • Discussion: 'Maternal hypotension during cesarean section' by Maayan-Metzger et al.

    abstract::In the roundtable that follows, clinicians discuss a study published in this issue of the Journal in light of its methodology, relevance to practice, and implications for future research. Article discussed: Maayan-Metzger A, Schushan-Eisen I, Todris L, et al. Maternal hypotension during elective cesarean section and s...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 评论,杂志文章

    doi:10.1016/j.ajog.2009.11.012

    authors: Blackwell SC,Pacheco L,Chiossi G,Lee T,Nguyen T

    更新日期:2010-01-01 00:00:00

  • Production of inhibin A and inhibin B in human ovarian sex cord stromal tumors.

    abstract:OBJECTIVE:Our purpose was to examine the cellular localization of inhibin subunits and messenger ribonucleic acid expressions for the inhibin subunits and the serum levels of inhibin A and inhibin B in human ovarian sex cord stromal tumors. STUDY DESIGN:We examined the immunohistochemical localization of the inhibin s...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/s0002-9378(97)70090-5

    authors: Yamashita K,Yamoto M,Shikone T,Minami S,Imai M,Nishimori K,Nakano R

    更新日期:1997-12-01 00:00:00

  • The effects of magnesium sulfate infusion on blood pressure and vascular responsiveness during pregnancy.

    abstract::Magnesium sulfate is primarily used for its anticonvulsive effects in treating hypertensive disorders of pregnancy. However, we have frequently observed significant falls in blood pressure in patients suffering from severe pregnancy-induced hypertension in whom little or no additional hypotensive medication was necess...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(84)90106-6

    authors: Lee MI,Todd HM,Bowe A

    更新日期:1984-08-01 00:00:00

  • Puerperal infection after cesarean delivery: evaluation of a standardized protocol.

    abstract:OBJECTIVE:Our goal was to evaluate an antibiotic protocol for treatment of postcesarean endometritis. STUDY DESIGN:Endometritis was diagnosed as a persistent fever > or =100.4 degrees F beyond 24 hours after cesarean delivery and one or more of the following: uterine tenderness, tachycardia, foul lochia, or leukocytos...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1067/mob.2000.103249

    authors: Brumfield CG,Hauth JC,Andrews WW

    更新日期:2000-05-01 00:00:00

  • Lupus anticoagulant and pregnancy.

    abstract::A subset of women with a high rate of fetal wastage is identifiable among those with serologic but not necessarily clinical evidence of connective tissue disease. The presence of lupus anticoagulant in the plasma of a pregnant woman serves as a marker for a high rate of fetal wastage and risk of thrombosis. Lupus anti...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章,评审

    doi:10.1016/s0002-9378(85)80124-1

    authors: Lubbe WF,Liggins GC

    更新日期:1985-10-01 00:00:00

  • Chorioamnionitis induced by intraamniotic lipopolysaccharide resulted in an interval-dependent increase in central nervous system injury in the fetal sheep.

    abstract:OBJECTIVE:We quantified the impact of chorioamnionitis on both the white and gray matter structures of the preterm ovine central nervous system (CNS). STUDY DESIGN:The CNS was studied at 125 days of gestation, either 2 or 14 days after the intraamniotic administration of 10 mg of lipopolysaccharide (LPS) (Escherichia ...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2008.12.003

    authors: Gavilanes AW,Strackx E,Kramer BW,Gantert M,Van den Hove D,Steinbusch H,Garnier Y,Cornips E,Steinbusch H,Zimmermann L,Vles J

    更新日期:2009-04-01 00:00:00

  • Lymphokine production during term human pregnancy: differences between peripheral leukocytes and decidual cells.

    abstract::Although the fetus is considered to be an "allograft" there is little information concerning the role of lymphokines in human pregnancy. Lymphokines are polypeptides secreted by stimulated lymphocytes that direct the immune response by enabling immune effector cells to communicate with each other. To characterize lymp...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(90)90769-4

    authors: Dudley DJ,Mitchell MD,Creighton K,Branch DW

    更新日期:1990-12-01 00:00:00

  • Prenatal diagnosis of autosomal recessive polycystic kidney disease: variable outcome within one family.

    abstract::Autosomal recessive polycystic kidney disease is frequently diagnosed in utero by obstetric ultrasonography. We report a case in which there were varying outcomes of this disorder in three affected fetuses in a family. Recognition of variable expression within one family is important when parents are considering termi...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(92)91672-w

    authors: Barth RA,Guillot AP,Capeless EL,Clemmons JJ

    更新日期:1992-02-01 00:00:00

  • Recurrence of placental dysfunction disorders across generations.

    abstract:OBJECTIVE:Knowledge about the causes of placental dysfunction disorders is limited. We performed an intergenerational study, focusing on the risks of placental dysfunction disorders in mothers and fathers who had been born small for gestational age (SGA). STUDY DESIGN:Using linked generational data from the Swedish Me...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2011.05.009

    authors: Wikström AK,Svensson T,Kieler H,Cnattingius S

    更新日期:2011-11-01 00:00:00

  • Postoperative pain after laparoscopic and vaginal hysterectomy for benign gynecologic disease: a randomized trial.

    abstract:OBJECTIVE:To compare postoperative pain after laparoscopic and vaginal hysterectomy for benign disease. STUDY DESIGN:A prospective randomized trial was designed to compare laparoscopic hysterectomy and vaginal hysterectomy in patients with uterine volume

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.ajog.2010.04.026

    authors: Ghezzi F,Uccella S,Cromi A,Siesto G,Serati M,Bogani G,Bolis P

    更新日期:2010-08-01 00:00:00

  • Detection of genes that are differentially expressed during mouse embryogenesis by genetic trapping strategies.

    abstract:OBJECTIVE:Our objective was to identify novel genes that are expressed in temporally and spatially restricted patterns during mouse embryonic development and organogenesis. STUDY DESIGN:Two genetic trapping reporter constructs that lack transcriptional regulatory sequences were introduced independently into transcript...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(93)90643-w

    authors: Johnson MD,Mahon KA

    更新日期:1993-09-01 00:00:00

  • Glucocorticoids and the rhesus fetal lung.

    abstract::The effects of glucocorticoids on primate fetal lung function have not been clearly delineated. In this prospective study of preterm rhesus fetuses exposed in utero to betamethasone for 72 hours, the most significant alteration was a striking increase in maximum lung volumes. Functionally less significant increases in...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(78)90267-3

    authors: Johnson JW,Mitzner W,London WT,Palmer AE,Scott R,Kearney K

    更新日期:1978-04-15 00:00:00

  • Breast aspiration biopsy with multihole needles for histologic and cytologic examination.

    abstract::Multihole needle biopsy has been performed on 166 patients. Commercially available 22-, 20-, 18-, and 16-gauge hypodermic needles (40 to 75 mm in length) are prepared with three sharp-edged holes around the distal part of the needle, allowing aspiration of 3 to 6 ml or more of tissue, for both histologic and cytologic...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(85)90427-2

    authors: Vorherr H

    更新日期:1985-01-01 00:00:00

  • Vaginal birth after cesarean section: the impact of patient resistance to a trial of labor.

    abstract::In spite of the relative safety and medical advantages of vaginal birth after cesarean section, the procedure continues to be underutilized in the private practice setting. To evaluate the hypothesis that resistance by the patient often precludes a trial of labor, an observational study was conducted of all women with...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(91)91422-s

    authors: Joseph GF Jr,Stedman CM,Robichaux AG

    更新日期:1991-06-01 00:00:00

  • Analysis of 500 obstetric and gynecologic malpractice claims: causes and prevention.

    abstract::Five hundred obstetric and gynecologic malpractice litigation claims were analyzed to determine the incidence of claims that could not be defended because of breach of standards of care, problems with documentation, or both. Of the 500 claims, 294 were obstetric and 206 were gynecologic. Of the obstetric claims, 79 (2...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(91)90082-3

    authors: Ward CJ

    更新日期:1991-08-01 00:00:00

  • Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility.

    abstract::We have undertaken a prospective analysis of the diagnostic significance of three different criteria of human sperm function including the conventional semen profile, measurements of hamster-oocyte fusion, and determinations of reactive oxygen species generation in 139 couples. The latter, who were characterized by a ...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/s0002-9378(11)80017-7

    authors: Aitken RJ,Irvine DS,Wu FC

    更新日期:1991-02-01 00:00:00

  • Methadone trough levels in pregnancy.

    abstract:OBJECTIVE:This study was undertaken to determine (1) the methadone serum trough level adequate to prevent withdrawal symptoms in heroin-addicted pregnant women and (2) whether the methadone serum trough level in symptomatic women with withdrawal symptoms differs from that of asymptomatic women. STUDY DESIGN:Pregnant w...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1067/mob.2002.127132

    authors: Drozdick J 3rd,Berghella V,Hill M,Kaltenbach K

    更新日期:2002-11-01 00:00:00

  • Effects of hormone replacement therapy on the mammary gland of surgically postmenopausal cynomolgus macaques.

    abstract:OBJECTIVE:Our purpose was to define the proliferative response and receptor status in the mammary glands of surgically postmenopausal macaques given hormone replacement therapy, equivalent for monkeys to that given women. STUDY DESIGN:Surgically postmenopausal adult female cynomolgus macaques (Macaca fascicularis) wer...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/s0002-9378(96)70379-4

    authors: Cline JM,Soderqvist G,von Schoultz E,Skoog L,von Schoultz B

    更新日期:1996-01-01 00:00:00

  • Burnout in chairs of obstetrics and gynecology: diagnosis, treatment, and prevention.

    abstract:OBJECTIVE:The purpose of this study was to determine the prevalence of burnout in chairs of academic departments of obstetrics and gynecology, identify important stressors, and develop strategies to treat and prevent burnout. STUDY DESIGN:We performed a cross-sectional study of 131 chairs in the United States and Puer...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1067/mob.2002.122391

    authors: Gabbe SG,Melville J,Mandel L,Walker E

    更新日期:2002-04-01 00:00:00

  • Dystocia increases with advancing maternal age.

    abstract:OBJECTIVE:The purpose of this study was to assess the influence of maternal age on obstetric indices of uterine efficiency in spontaneous nulliparous labor managed according to a standardized protocol in order to determine whether increasing maternal age is more commonly associated with dystocia. STUDY DESIGN:Informat...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/j.ajog.2006.05.052

    authors: Treacy A,Robson M,O'Herlihy C

    更新日期:2006-09-01 00:00:00

  • Disposition of meperidine and normeperidine following multiple doses during labor. II. Fetus and neonate.

    abstract::It has been suggested that continued diffusion gradients from mother to fetus would exist and that both meperidine and normeperidine would accumulate in the fetus following multiple doses of meperidine to the mother during labor. However, no pharmacokinetic data are available. Therefore, the purpose of this study was ...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0002-9378(85)90314-x

    authors: Kuhnert BR,Kuhnert PM,Philipson EH,Syracuse CD

    更新日期:1985-02-01 00:00:00

  • Defining the role of fluorescence in situ hybridization on uncultured amniocytes for prenatal diagnosis of aneuploidies.

    abstract:OBJECTIVE:This study examines the role of fluorescence in situ hybridization on uncultured amniocytes for prenatal diagnosis in a population at high risk for aneuploidies. STUDY DESIGN:All patients undergoing amniocentesis for fetal structural abnormality on ultrasonographic examination (performed from 13 to 39 weeks)...

    journal_title:American journal of obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/s0002-9378(97)70600-8

    authors: D'Alton ME,Malone FD,Chelmow D,Ward BE,Bianchi DW

    更新日期:1997-04-01 00:00:00