Abstract:
BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), some ubiquitous environmental contaminants are capable to cause oxidative stress, during its metabolism. It is believed that many diseases that have a common origin in oxidative stress begin in childhood. Considering oxidative stress evolved during PAHs metabolism as one main mechanism responsible for health hazards related to PAHs exposure in children, we biomonitored blood PAHs levels in connection with redox status among children of Lucknow (India). METHODS:The study consisted of children (n = 50) who visited to the Pediatrics Department (KGMU) Lucknow for usual health check-up camp over the study period (August 2005-July 2006). Blood samples were drawn and levels of acenaphthylene, anthracene, phenanthrene, fluoranthene, naphthalene, pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene and benzo(a)pyrene were determined by HPLC-FD/UV. Malondialdehyde (MDA), glutathione (GSH) levels, catalase (CAT) and superoxide dismutase (SOD) activity were also determined to evaluate redox status. RESULTS:Significantly elevated carcinogenic blood PAHs levels (125.55 +/- 26.99ppb, p < 0.05) were found in rural children compared to children from urban region (23.96 +/- 13.46). Results revealed that remoteness between residence and highway/traffic, significantly influences the blood levels of carcinogenic PAHs. There were significant correlations between total PAHs and MDA (r = 0.82, p < 0.001), carcinogenic PAHs and SOD activity (r = 0.35, p < 0.01), Total PAHs and blood GSH level (r = - 0.49, p < 0.01) and carcinogenic PAHs and CAT activity (r = 0.42, p < 0.01). Blood MDA level was also found correlated with increasing body mass index (BMI) (r = 0.29, p < 0.05). CONCLUSION:Our results showed blood PAHs levels in children significantly correlated with oxidative stress and altered antioxidant status. It supports our hypothesis that the children exposed to high PAHs level will suffer more to oxidative stress that may lead to possible health risks. Additional studies with large sample size are considered necessary to strengthen the database and also to explore the PAHs associated health risks in children.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Singh VK,Patel DK,Jyoti,Ram S,Mathur N,Siddiqui MKdoi
10.1016/j.clinbiochem.2007.11.017subject
Has Abstractpub_date
2008-02-01 00:00:00pages
152-61issue
3eissn
0009-9120issn
1873-2933pii
S0009-9120(07)00476-6journal_volume
41pub_type
杂志文章abstract::Fourteen antirheumatoid drugs were tested for their effects on the in vitro hyaluronidase activity of normal human serum. Four drugs produced significant changes in enzyme activity. Different results were obtained with ovine testicular hyaluronidase when diluted with either saline or inactivated human serum. No increa...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(76)80053-7
更新日期:1976-08-01 00:00:00
abstract::The toxicity of carbon monoxide has been recognized for long throughout history and is unquestionably the leading cause of unintentional poisoning deaths in the Western countries. The severity of poisoning is dependent upon environmental and human factor. The leading pathophysiological mechanism resides in the ability...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
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更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:We evaluated the analytical performance of the Elecsys 2010 immunoanalyzer (Boehringer Mannheim Canada), which is based on a new detection technology, electrochemical luminescence. DESIGN AND METHODS:We used six representative assays from the initial launch menu of the instrument: thyroid stimulating hormon...
journal_title:Clinical biochemistry
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doi:10.1016/s0009-9120(98)00002-2
更新日期:1998-03-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of Matrix metalloproteinase 7 -181A/G polymorphism (rs11568818) on the risk of cancer, we performed a meta-analysis. DESIGN AND METHODS:24 case-control studies were identified by searching Pubmed, Embase, ISI, OVID MEDLINE and EBSCO databases. Odd ratios and corresponding 95% confiden...
journal_title:Clinical biochemistry
pub_type: 杂志文章,meta分析
doi:10.1016/j.clinbiochem.2013.07.015
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVES:End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.11.011
更新日期:2014-04-01 00:00:00
abstract::The paraoxonases (PON1, PON2 and PON3) are an enzyme family with a high structural homology. All of them have lactonase activity and degrade lipid peroxides in lipoproteins and cells. As such, they play a role in protection against oxidation and inflammation. Infectious diseases are often associated with oxidative str...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2017.04.016
更新日期:2017-09-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(81)90976-0
更新日期:1981-10-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...
journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2009.07.023
更新日期:2009-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(81)90211-3
更新日期:1981-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(02)00380-6
更新日期:2002-10-01 00:00:00
abstract::The synthesis and characterisation of a new colorimetric reagent for iron, 3-(2-pyridyl)-5,6-bis(2-furyl)-1,2,4-triazine are described. Peak assignments and chemical shifts of the high resolution 13C nmr spectrum are given. The reagent forms a tris iron(II) complex with epsilon max of 32000 Lcm-1 mol-1 at 577 nm. The ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(84)90613-1
更新日期:1984-10-01 00:00:00
abstract::This retrospective multicenter study in patients with chronic myeloid leukemia in chronic phase was undertaken to confirm the clinical relevance of imatinib plasma concentrations monitoring in daily practice. Forty-one patients, with 47 imatinib plasma measurements, were analyzed during treatment with imatinib given a...
journal_title:Clinical biochemistry
pub_type: 杂志文章,多中心研究
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更新日期:2017-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(02)00314-4
更新日期:2002-06-01 00:00:00
abstract::A highly specific enzyme-linked "sandwich" immunoassay was developed for determining cardiac myosin light chain II (MLC II) in serum by using an anticardiac MLC II monoclonal antibody and a solid phase consisting of glass rods coated with another monoclonal antibody. We can detect as little as 0.2 ng of cardiac MLC II...
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pub_type: 杂志文章
doi:10.1016/0009-9120(90)80042-h
更新日期:1990-12-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.07.015
更新日期:2008-10-01 00:00:00
abstract::A fluorescence polarization (FP) technique was applied to the assay of lysozyme activity in human urine. In this assay system, reaction between enzyme and fluorescein-labelled substrate produced a decrease in the fluorescein polarization (P) value. Enzyme activity and rate of decrease of P value exhibited a hyperbolic...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(85)80022-9
更新日期:1985-02-01 00:00:00
abstract::We compared results of urine drug analysis with clinical data and history to test the usefulness of peripartum drug screening and to establish guidelines for optimal testing. Urine from 28 mothers and 52 babies was analysed. Drugs not suspected by history were found in 10 mothers and six babies. Results assisted in th...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(88)80113-9
更新日期:1988-01-01 00:00:00
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journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2013.07.022
更新日期:2013-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.01.002
更新日期:2013-05-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.08.013
更新日期:2006-12-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2014.01.033
更新日期:2014-06-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.04.008
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVE:To quantify pepsinogen C (PEPC) and prostaglandin D synthase (PGDS) in breast cyst fluid and examine if these two parameters can be used for breast cyst type classification. DESIGN AND METHODS:We quantified PEPC and PGDS in 92 and 50 breast cyst fluids, respectively, using previously established immunofluoro...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(98)00079-4
更新日期:1999-02-01 00:00:00
abstract::The use of point-of-care (POC) immunoassays has increased significantly and the menu of analytes continues to expand. Most of the rapid immunoassays are currently based on simple manual assay devices such as the immunochromatographic, agglutination, and immunofiltration assays. Although automated readers have recently...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2005.03.008
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:To assess the relationship between lipoprotein (a) [Lp (a)] and lipoprotein oxidation in patients with coronary artery disease (CAD). DESIGN AND METHODS:Oxidation of apolipoprotein (apo)B-containing lipoproteins, vitamin E, carotenoids, lipid-lipoprotein levels were determined in 171 CAD and 70 non-CAD subj...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(00)00079-5
更新日期:2000-06-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2011.12.024
更新日期:2012-03-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/0009-9120(91)80014-t
更新日期:1991-06-01 00:00:00
abstract:OBJECTIVES:To study the usefulness of combined cardiac Troponin T (cTnT) and CK-MB mass determinations in risk stratification of acute coronary syndromes. DESIGN AND METHODS:Blood samples for cTnT and CK-MB mass were collected at arrival and 4, 8, and 12-24 later in 301 consecutive patients with recent acute chest pai...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2003.12.003
更新日期:2004-04-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1016/s0009-9120(05)80009-8
更新日期:1991-12-01 00:00:00