Abstract:
:An important component of nephrology research is the discovery of novel proteins that control cellular and molecular events that contribute to normal kidney cell biology and disease. Identifying perturbation of normal cellular protein expression and interactions within signaling networks is critical for understanding these regulatory events. Methods that couple 2-dimensional capillary liquid chromatography and tandem mass spectrometry (2D-LC-MS/MS) analysis have greatly facilitated this discovery science. Coupling 2D-LC-MS/MS analysis with automated genome-assisted spectra interpretation allows a direct, high-throughput, and high-sensitivity identification of hundreds to thousands of individual proteins from targeted complex biological samples. The systematic qualitative and quantitative comparison of experimental/disease conditions and appropriate controls allow protein function or disease states to be modeled. This review discusses the different purification and quantitative strategies that have been developed and used in combination with 2D-LC-MS/MS and computational analysis to define regulatory events in kidney biology and disease.
journal_name
Semin Nephroljournal_title
Seminars in nephrologyauthors
Cummins TD,Powell DWdoi
10.1016/j.semnephrol.2007.09.008subject
Has Abstractpub_date
2007-11-01 00:00:00pages
574-83issue
6eissn
0270-9295issn
1558-4488pii
S0270-9295(07)00122-2journal_volume
27pub_type
杂志文章,评审abstract::In contrast to managing patients on hemodialysis in whom iron strategies are more focused on intravenous iron, nondialysis chronic kidney disease (CKD) patients may receive either oral or intravenous iron. There are advantages and disadvantages for both strategies. Oral iron is simple and cheap to administer and does ...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2016.02.003
更新日期:2016-03-01 00:00:00
abstract::The renal biopsy provides critical diagnostic and prognostic information to clinicians including cases of acute kidney injury, chronic kidney disease, and allograft dysfunction. Today, biopsy specimens are read using a combination of light microscopy, electron microscopy, and indirect immunofluorescence, with a limite...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2017.09.005
更新日期:2018-01-01 00:00:00
abstract::Compared with Caucasians, African Americans have lower circulating concentrations of 25-hydroxyvitamin D (25(OH)D), the major storage form of vitamin D, leading to the widespread assumption that African Americans are at higher risk of vitamin D deficiency. However, the finding that African Americans maintain better in...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2013.07.006
更新日期:2013-09-01 00:00:00
abstract::The search for an endogenous digitalis has led to the identification of the cardenolides ouabain and digoxin and the bufadienolide marinobufagenin in mammalian tissues and biological fluids. Ouabain's release from adrenal glands is under the control of epinephrine and angiotensin II; hence, its blood concentration cha...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2005.03.010
更新日期:2005-09-01 00:00:00
abstract::The incidence of progressive kidney disease associated with diabetes continues to increase worldwide. Only partial renoprotection is achieved by current standard therapy with angiotensin-converting enzyme inhibitors and/or angiotensin-receptor blockers, increasing the need for novel therapeutic approaches. Experimenta...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2015.03.003
更新日期:2015-03-01 00:00:00
abstract::Patients with cirrhosis have significant abnormalities in their fluid and electrolyte balance, this is manifested mainly by the development of ascites and edema. Ascites is the most common complication of patients with cirrhosis and its development constitutes the first and most important manifestation of the disease....
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1053/snep.2001.21666
更新日期:2001-05-01 00:00:00
abstract::Macrophages have long been regarded as classic mediators of innate immunity because of their production of proinflammatory cytokines and their ability to induce apoptotic cell death. As a result of such activities and the detrimental long-term effect of kidney inflammation, macrophages principally have been regarded a...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2010.03.011
更新日期:2010-05-01 00:00:00
abstract::An expanding proportion of people in the United States and worldwide are affected by kidney disease, leading to a growing concern over the public health implications. Despite the high prevalence and the considerable associated health risks of kidney disease, major gaps in our knowledge base hinder the delivery of opti...
journal_title:Seminars in nephrology
pub_type: 评论,杂志文章,评审
doi:10.1016/j.semnephrol.2009.06.002
更新日期:2009-09-01 00:00:00
abstract::Hotspots of chronic kidney disease of unknown etiology (CKDu) have been identified throughout the globe, of which the Mesoamerican nephropathy in Central America is the most conspicuous example. It affects mainly agricultural workers, heat exposure during extenuating shifts leading to sudden dehydration and subsequent...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2019.02.008
更新日期:2019-05-01 00:00:00
abstract::Hypertension is growing in prevalence and most of this growth represents isolated systolic hypertension in the elderly. Attempts at prevention are critical but likely are ineffectual. Therefore, the delivery and acceptance of current medical regimens must be improved. ...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2005.02.002
更新日期:2005-07-01 00:00:00
abstract::In humans, idiopathic hypercalciuria is associated with stone formation. To study the mechanisms that are responsible for the excess urine calcium excretion in ways that are difficult to impossible in humans, we have developed a rat model of idiopathic hypercalciuria. Hypercalciuric rats were successively inbred for m...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1996-09-01 00:00:00
abstract::Premature menopause defined as secondary amenorrhea is common in women with kidney disease. Moreover, the aging population has resulted in increasing numbers of postmenopausal women with kidney disease. Though the pathophysiology is poorly understood, kidney transplantation and more frequent hemodialysis can restore m...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2017.05.013
更新日期:2017-07-01 00:00:00
abstract::The vacuolar H(+)-ATPase is a multisubunit protein consisting of a peripheral catalytic domain (V(1)) that binds and hydrolyzes adenosine triphosphate (ATP) and provides energy to pump H(+) through the transmembrane domain (V(0)) against a large gradient. This proton-translocating vacuolar H(+)-ATPase is present in bo...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2006.07.004
更新日期:2006-09-01 00:00:00
abstract::Sphingolipids, including ceramides, glycosphingolipids, sphingomyelin, and sphingosine-1-phosphate, have been recognized as important molecules that regulate critical cellular functions. Although originally studied in the context of lysosomal storage diseases, the roles of these compounds in more common disorders invo...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2018.01.007
更新日期:2018-03-01 00:00:00
abstract::We related 24-hour sodium and potassium excretion cross-sectionally to blood pressure and longitudinally to coronary events and deaths during follow-up in a representative sample of the Scottish population. A random sampling of men and women aged 40-59 in 1984 to 1987 produced 11,629 men and women who provided questio...
journal_title:Seminars in nephrology
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::The epidemiology of infection-related glomerulonephritis is undergoing striking changes, particularly in developed countries. The incidence of acute poststreptococcal glomerulonephritis (PSAGN) is decreasing because of the successful treatment of streptococcal infections. In contrast, because of the emergence of antib...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2011.06.008
更新日期:2011-07-01 00:00:00
abstract::Chronic kidney disease-mineral and bone disorder (CKD-MBD) is characterized by bone abnormalities, vascular calcification, and an array of laboratory abnormalities. The latter classically include disturbances in the parathyroid hormone/vitamin D axis. More recently, fibroblast growth factor 23 (FGF23) and klotho also ...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2014.02.007
更新日期:2014-03-01 00:00:00
abstract::Despite intensive research over the past three decades, the events leading to pathogenic autoantibody production and immune deposit formation in individuals with systemic lupus erythematosus continues to be debated. The controversy is fueled by the clinical observations that individual patients with lupus have variabl...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::At the present time, knowledge of the structure of the proton-translocating ATPase responsible for urinary acidification is far from complete. Key issues awaiting resolution are full definition of subunit structure and an understanding of the interactions among functional domains of this complex hetero-oligomer. Such ...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1990-03-01 00:00:00
abstract::Although calcitriol has been shown to have an important role in the pathogenesis of hyperparathyroidism, its use as a therapeutic agent often has been limited by calcemic and phosphatemic toxicity. Vitamin D analogs and the synthetic prohormones, with the potential to have lesser effects on calcium and phosphorus, hav...
journal_title:Seminars in nephrology
pub_type: 杂志文章
doi:10.1016/j.semnephrol.2004.06.013
更新日期:2004-09-01 00:00:00
abstract::Initially described in 1984, human immunodeficiency virus-associated nephropathy (HIVAN) has now become a common disease within the HIV-seropositive population. It is a focal segmental glomerulosclerosis causing rapid deterioration of renal function. It is the most common cause of chronic renal disease in HIV patients...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1053/snep.2003.50018
更新日期:2003-03-01 00:00:00
abstract::The rapid understanding of the cellular and molecular basis of organ function and disease processes will be translated in the next millennium into new therapeutic approaches to a wide range of clinical disorders, including acute and chronic renal failure. Central to these new therapies are the developing fields of gen...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Acute ingestion of protein and intravenous infusion of amino acids are known to stimulate increases in renal blood flow and glomerular filtration rate (GFR) in animals and humans. Although some investigators believe that these changes are mediated by a circulating hormonal factor, there is considerable evidence that i...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1995-09-01 00:00:00
abstract::Substantial progress has been made in the application of computer-driven methods to provide erythropoietic dosing information for patients with anemia resulting from chronic kidney disease. Initial solutions were simply computerized versions of traditional paper-based anemia management protocols. True personalization ...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2018.05.010
更新日期:2018-07-01 00:00:00
abstract::The renal tubular epithelium maintains different sets of transport proteins in plasma membrane domains facing the lumenal and mucosal fluids and limits diffusion between these two compartments. This asymmetry is generated by directing transporters to the appropriate membrane domain, a process that requires both an int...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1998-03-01 00:00:00
abstract::The first part of this article focuses on the risk of functional deterioration in subjects with solitary kidneys; the long-term clinical outcome of various subgroups of patients is reviewed. Thereafter, the pathophysiology of the renal functional reserve in subjects with a 50% reduction in renal parenchyma and the res...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1995-09-01 00:00:00
abstract::Toxic nephropathies cause acute and chronic renal failure, primarily as a result of injury to renal tubular epithelium. There is a well-known capacity in the renal nephron for the synchronous occurrence of both apoptosis and necrosis in toxic nephropathies. This has engendered interest in the differing or complementar...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/s0270-9295(03)00085-8
更新日期:2003-09-01 00:00:00
abstract::In aggregate, the evidence reviewed here supports a very important role for PDGF expression and action at local glomerular and interstitial sites in human kidney development and disease. PDGF delivered by platelets, or produced by endogenous cells of the kidney is capable of stimulating responses including proliferati...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1993-01-01 00:00:00
abstract::Immune-mediated nephritis is a common complication of systemic lupus erythematosus (SLE). It is now clear that multiple and independent mechanisms contribute to disease onset and pathogenesis, which may explain the remarkable phenotypic and histopathological heterogeneity observed in human SLE. Identification and char...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1999-03-01 00:00:00
abstract::Several studies have shown an association between the hemodialysis session length (the t of Kt or Kt/V) and favorable outcomes for patients on maintenance hemodialysis. In a single randomized controlled trial that systematically varied hemodialysis session length, shorter session length was associated with an increase...
journal_title:Seminars in nephrology
pub_type: 杂志文章,评审
doi:10.1016/j.semnephrol.2004.09.015
更新日期:2005-03-01 00:00:00