Abstract：
:Hirschsprung's disease is characterized by the absence of ganglion cells in the distal bowel and extends proximally for varying distances. In recent years, the purinergic P2Y receptors have begun to receive much attention as they have been recognised as major ATP receptors in many regions of the body, including the intestine. ATP has long been established as an inhibitory neurotransmitter in the enteric nervous system. The aim of our study was to analyse the expression of P2Y1 and P2Y2 receptors in the intestine of patients with Hirschsprung's disease. Frozen sections were cut from rectal tissue segments taken from both the aganglionic and ganglionic regions of patients with Hirschsprung's disease, as well as tissue from normal rectal biopsies, which were used as controls. Sections were incubated overnight with P2Y1 and P2Y2 receptor antibodies and results were analysed by light microscopy. Both P2Y1 and P2Y2 immunoreactivity was absent from the submucosal and myenteric plexuses of aganglionic tissue compared to ganglionic tissue and normal controls, in which large number of immunoreactive neurons were evident, arranged in ganglia in both plexuses and positive nerve fibres, in both the smooth and circular muscles. Our results show a lack of expression of P2Y1 and P2Y2 receptors in the aganglionic gut in Hirschsprung's disease. The absence of these receptors suggests the absence of the inhibitory neurotransmitter ATP, which may help to explain the contracted state of the aganglionic gut in Hirschsprung's disease.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
O' Donnell AM,Puri Pdoi
10.1007/s00383-007-2044-1subject
Has Abstractpub_date
2008-01-01 00:00:00pages
77-80issue
1eissn
0179-0358issn
1437-9813journal_volume
24pub_type
杂志文章abstract：PURPOSE:Lgr5+ intestinal epithelial stem cells (ISCs) crucial for intestinal epithelial regeneration are impaired during necrotizing enterocolitis. This study aims to investigate the influence of different stressors on intestinal epithelial injury and regeneration in vitro. METHODS:Intestinal epithelial cells (IEC-18)...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-017-4183-3
更新日期：2018-02-01 00:00:00
abstract：:Foreign-body (FB) aspirations account for a reasonable percentage of respiratory emergencies in young children. Whereas 67% of aspirated FBs are of organic origin, metallic-ferromagnetic FBs are observed less frequently. We report the use of a wire with a magnetic tip fitting into the working channel of a rigid bronch...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/BF01076970
更新日期：1997-07-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate the applicability of the National Institute for Health and Clinical Excellence (NICE) guidelines for imaging studies in children under the age of three with first urinary tract infection (UTI). METHODS:In our cohort of 112 patients, we gathered data regarding the occur...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-012-3257-5
更新日期：2013-03-01 00:00:00
abstract：:To evaluate the efficacy of ultrasound (US) guided percutaneous sclerotherapy in the pediatric population, 14 hydatid liver cysts (HLC) in eight male patients whose ages ranged between 6 and 16 years (mean 9.9 years) were treated. The maximum diameter was 110 mm. Albendazole was administered orally to all patients for...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830000356
更新日期：2000-01-01 00:00:00
abstract：:An unrecognised right aortic arch (RAA) found at thoracotomy may complicate the repair of oesophageal atresia (OA) and tracheo-oesophageal fistula (TOF). This paper analyses the patient characteristics, peri-operative management, and outcome of 16 infants with a RAA, and proposes management guidelines. Between 1948 an...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050505
更新日期：1999-01-01 00:00:00
abstract：:A thoracic mass was identified on a routine ultrasound (US) examination in the 30th week of gestation. A postnatal chest radiograph, US, and computed tomography demonstrated a well-delineated, solid, cystic tumor in the left lower lobe. The mass was excised and a diagnosis of pulmonary blastoma (PB) was made on pathol...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/BF00497817
更新日期：1996-06-01 00:00:00
abstract：:To determine which laser can be used for a vascular lesion, the different working mechanisms of the individual lasers must first be understood. The localization and diameter of the vessels to be treated define whether a certain laser is appropriate for that lesion. For teleangiectasias and port-wine stains the pulsed-...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/BF00497797
更新日期：1996-06-01 00:00:00
abstract：:Allgrove syndrome is a rare genetic disorder typically manifested by alacrima, achalasia, and adrenal insufficiency, and is one of the rare causes of achalasia in infants. While the gold standard for achalasia treatment in adults is an esophageal myotomy with fundoplication, a standard treatment for infantile achalasi...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-014-3493-y
更新日期：2014-06-01 00:00:00
abstract：:The most common cystic lesion recognized antenatally is multicystic dysplastic kidney (MCDK). Recently, conservative management without nephrectomy has been advocated. The purpose of this study was to report our experience in the conservative management of unilateral MCDK. Between 1989 and 1997, 20 children with MCDK ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830000449
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:We evaluated the effectiveness of prophylactic mesenteric lymphatic ligation in preventing iatrogenic chylous fistulae in abdominal neuroblastoma surgery. METHOD:All patients who underwent resection of abdominal neuroblastoma between years 2002 and 2013 were included. A change in surgical strategy commenced fr...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-014-3581-z
更新日期：2014-10-01 00:00:00
abstract：PURPOSE:To study the effects of frenulotomy on nipple pain, latch and the success in exclusive breastfeeding (EBF) at 3-month follow-up. METHODS:A prospective cross-sectional study of 328 mother-infant pairs with both tongue-tie and breastfeeding problems was performed. Nipple pain and latch were evaluated using numer...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-016-3952-8
更新日期：2016-10-01 00:00:00
abstract：:The goal of this article is to review the status of cultured epithelial autografts in clinical practice with particular focus on the pediatric subset of patients. The current indications include massive deep burns (>60 - 70% total body surface area), resurfacing-type postburn scar revisions, and skin defect coverage f...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/BF01258705
更新日期：1997-09-01 00:00:00
abstract：PURPOSE:The aim of this study is to identify the risk factors for esophageal anastomotic stricture (EAS) and/or anastomotic leakage (EAL) after primary repair of esophageal atresia with tracheoesophageal fistula (EA/TEF) in infants. METHODS:A retrospective chart review of 52 patients with congenital EA/TEF between Jan...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-016-3931-0
更新日期：2016-09-01 00:00:00
abstract：:Traditionally, open repair of esophageal atresia (EA) with tracheoesophageal fistula (TEF) required thoracotomy. Innovations in minimal access surgery have created a thoracoscopic technique resulting in violation of the pleural space. Most pediatric surgeons favor an extrapleural approach for open repair. We present a...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-005-1366-0
更新日期：2005-04-01 00:00:00
abstract：:A neonate with gastroschisis had silo placement and near-infrared spectroscopy (NIRS) monitoring of intestinal haemoglobin oxygen saturation (RSO2). An ischemic loop of bowel demonstrated decreased RSO2, with reversal of clinical ischemia and RSO2 postoperatively. This demonstrates possible advantage of monitoring int...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-014-3591-x
更新日期：2014-11-01 00:00:00
abstract：INTRODUCTION:Hepatoportoenterostomy (HPE) with the Kasai procedure is the treatment of choice for biliary atresia (BA) as the initial surgery. However, the appropriate level of dissection level of the fibrous cone (FC) of the porta hepatis (PH) is frequently unclear, and the procedure sometimes results in unsuccessful ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-015-3799-4
更新日期：2015-12-01 00:00:00
abstract：PURPOSE:To date, no detailed study of the changes in the image-defined risk factors (IDRFs) after neoadjuvant chemotherapy has been performed. The aim of this study was to investigate the effect of chemotherapy on IDRFs for stage L2 neuroblastomas. METHODS:Fifteen stage L2 patients treated by neoadjuvant chemotherapy ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-016-3858-5
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:The Mac-2-binding protein glycosylation-modified isomer (M2BPGi) is a new marker for progression of hepatic fibrosis. We examined the relationship between serum M2BPGi levels and liver histological findings in patients with biliary atresia (BA) who were not transplant candidates. METHODS:Patients with BA who w...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-018-4317-2
更新日期：2018-10-01 00:00:00
abstract：PURPOSE:Fetal extraperitoneal rectal perforation (FERP) is an extremely rare entity. The objective of this report is to review the available literature on this condition and to add our experience with four additional cases managed at our institution. METHODS:A literature search was performed for journal articles addre...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-019-04514-0
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:To compare the safety and efficacy between laparoscopic and open cyst excision with hepaticojejunostomy for children with choledochal cysts using meta-analysis. METHODS:Studies comparing the laparoscopic and the open choledochal cyst excision that met the inclusion criteria for data extraction were identifie...
journal_title：Pediatric surgery international
pub_type： 杂志文章,meta分析
doi：10.1007/s00383-015-3705-0
更新日期：2015-06-01 00:00:00
abstract：:Confirmation of the clinical diagnosis of Hirschsprung's disease on standard rectal suction biopsy requires demonstration of aganglionosis in 60 adequate serial sections of submucosa. Positive staining for acetylcholinesterase (AChE), demonstrating an increase in nerve fibres within the lamina propria, muscularis muco...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050237
更新日期：1998-01-01 00:00:00
abstract：:Recent evidence suggests that neutrophil recruitment may initiate germ cell apoptosis in the ischemic testis. The purpose of the present study was to examine the relationship between germ cell apoptosis and neutrophil recruitment in the contralateral testis following testicular ischemia-reperfusion (IR) injury in a ra...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-006-1854-x
更新日期：2007-05-01 00:00:00
abstract：:We present three cases of abdominal and abdomino-thoracic extra-lobar pulmonary sequestrations detected by antenatal ultrasound (US) over a 12-month period. In addition, we briefly refer to a case previously described. A cystic mass was demonstrated in the left upper abdomen of the fetus in all four cases, with two ex...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/BF00183739
更新日期：1996-03-01 00:00:00
abstract：:Gastroschisis closure is performed either primarily or after staged reduction of the prolapsed bowel. A technique for surgical closure of the abdominal wall defect is described which allows for an almost scarless appearance of the abdominal surface with preservation of a midline umbilicus. ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-008-2215-8
更新日期：2008-09-01 00:00:00
abstract：:Port-site recurrence (PSR) following laparoscopic procedures has been an unpredictable complication in adult cancer patients; however, no data exist about this phenomenon in the pediatric field. The aim of this study was to determine whether PSR, following endosurgical procedure for malignancies, is a typical complica...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-002-0918-9
更新日期：2003-05-01 00:00:00
abstract：INTRODUCTION:The Morgagni hernia (MH) accounts for 3-4% of congenital diaphragmatic hernias. There is a paucity of data regarding this rare defect. The purpose of this study is to describe the characteristics of children with MH, surgical approaches for repair, and patient outcomes. METHODS:Pediatric patients (ages 0-...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-017-4078-3
更新日期：2017-07-01 00:00:00
abstract：:Cryptorchidism is a common paediatric complaint and is traditionally managed surgically by orchidopexy. We aimed to prospectively review the success of the Bianchi high scrotal incision technique for performing orchidopexy in boys with palpable non-descended testis. A total of 206 orchidopexies were performed in 85 pa...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-008-2149-1
更新日期：2008-06-01 00:00:00
abstract：:Peri-catheter calcification is an unusual and previously unreported complication of central venous (CV) catheterization in infants. A 1. 9 Fr Silastic CV catheter was placed in a term infant for administration of total parenteral nutrition and antibiotics following intra-abdominal sepsis. The catheter was removed, wit...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050418
更新日期：1998-10-01 00:00:00
abstract：PURPOSE:This study evaluates the capacity of ultrasonography as a diagnostic method to confirm the proper positioning of central venous catheter (CVC) when compared to the current gold standard, chest radiography (CR). METHODS:A prospective study was performed including children from 0 to 14 incomplete years, who unde...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-020-04642-y
更新日期：2020-05-01 00:00:00
abstract：PURPOSE:To evaluate the role of bone marrow-derived mononuclear cells (BMC) in rat bile duct ligation (BDL) model. METHODS:Wistar rats were categorized into four Groups A-D. Normal liver biopsy was taken from Group A. BDL model was created in Groups B and C (15 each). Normal saline and BMC were injected through portal...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-013-3314-8
更新日期：2013-06-01 00:00:00
