Abstract:
:Confirmation of the clinical diagnosis of Hirschsprung's disease on standard rectal suction biopsy requires demonstration of aganglionosis in 60 adequate serial sections of submucosa. Positive staining for acetylcholinesterase (AChE), demonstrating an increase in nerve fibres within the lamina propria, muscularis mucosae, and subjacent submucosa, is a useful adjunctive test. In this study, sections of distal colonic muscularis propria and rectal mucosa were stained for AChE over a period of days following storage at 4 degrees C and at room temperature (15-20 degrees C). Positive staining of neural tissue was demonstrated in specimens stored at 4 degrees C for up to 14 days, at which time the experiment was discontinued due to tissue autolysis. Positive staining of the myenteric plexus in colonic specimens stored at room temperature also continued until tissue dissolution became marked at 5 days. This study has demonstrated stability of AChE staining of intestinal neural tissue in specimens stored at 4 degrees C for 14 days, which suggests that reliable staining for AChE should still be achievable if rectal biopsies are taken in clinics/hospitals without access to staining facilities, provided that tissues are transferred (fresh and moist, at 4 degrees C) to a reference laboratory for staining within several days of the biopsy procedure.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Byard RW,Carli Mdoi
10.1007/s003830050237subject
Has Abstractpub_date
1998-01-01 00:00:00pages
29-31issue
1eissn
0179-0358issn
1437-9813journal_volume
13pub_type
杂志文章abstract::With advances in neonatology, there is an increasing need for central vascular access in extremely small (<1,000 g) premature infants. Although the use of peripherally inserted central venous lines have become common practice, surgeons still frequently perform central venous line placements via cut-down in difficult a...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1474-x
更新日期:2005-09-01 00:00:00
abstract::There are few publications about urolithiasis of the new born baby and infant (UNI). The UNI represents 20% of the pediatric urolithiasis. The etiologies in this age group are chiefly dominated by the urinary-tract infections and metabolic abnormalities. The purpose of this paper was to investigate the epidemiological...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-007-1889-7
更新日期:2007-04-01 00:00:00
abstract:PURPOSE:Adhesive small bowel obstruction (ASBO) is one of the most important cause of postoperative morbidity in children who underwent abdominal surgery. Laparoscopic management for ASBO in pediatric patients has been reported. However, its safety and efficacy has not been evaluated in details. The aim of this study i...
journal_title:Pediatric surgery international
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00383-017-4186-0
更新日期:2018-02-01 00:00:00
abstract::We report a rare case of massive haematochezia due to hookworm infestation that required two laparotomies before the cause of bleeding could be diagnosed. The need to consider this parasite as a possible cause of gut bleeding in endemic countries is stressed, as early recognition may avoid unnecessary investigations a...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF00497827
更新日期:1996-06-01 00:00:00
abstract::Inflammatory pseudotumors (IPT) are uncommon mass lesions arising most typically in the lungs of young adults. These tumors are so named because of the difficulty in distinguishing them preoperatively from malignant lesions. IPT are characterized histologically by localized fibrous proliferations with infiltration by ...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s003830000518
更新日期:2001-09-01 00:00:00
abstract:INTRODUCTION:Bladder Bowel Dysfunction (BBD) has been described in patients with Down's Syndrome (DS). Our aim was to report the incidence, demographics, presentation, complications and management of the bladder in DS patients with BBD. METHODS:A systematic review was performed using PRISMA guidelines and search terms...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04682-4
更新日期:2020-07-01 00:00:00
abstract::The authors report two patients with extrahepatic portal hypertension and repeated massive bleeding from esophageal varices who underwent urgent distal splenorenal shunting (DSRS) after having proved refractory to medical treatment, endoscopic sclerotherapy, and ligation of esophageal varices. Their ages and weights w...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100014
更新日期:2001-11-01 00:00:00
abstract::To determine which laser can be used for a vascular lesion, the different working mechanisms of the individual lasers must first be understood. The localization and diameter of the vessels to be treated define whether a certain laser is appropriate for that lesion. For teleangiectasias and port-wine stains the pulsed-...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF00497797
更新日期:1996-06-01 00:00:00
abstract:OBJECTIVE:Several procedures have been described for repair of distal hypospadias. However, these are not without any complications. Corpus spongiosum advancement is a new technique employed in our patients for distal hypospadias repair with excellent clinical outcome and no post-operative complications. PATIENTS AND ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3292-x
更新日期:2013-06-01 00:00:00
abstract::Robotic assisted minimal invasive surgery (RMIS) is a new resource popular in some surgical specialties but not yet in pediatric surgery. There are numerous advantages of robotic surgical technology in adult patients well documented in the literature. The purpose of this study is to define the feasibility, safety, and...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2901-9
更新日期:2011-09-01 00:00:00
abstract:PURPOSE:Hirschsprung disease (HSCR) has previously been associated with increased mortality. The aim of this study was to assess mortality in patients with Hirschsprung disease in a population-based cohort. METHODS:This was a nationwide, population-based cohort study. The study exposure was HSCR and the study outcome ...
journal_title:Pediatric surgery international
pub_type: 杂志文章,多中心研究
doi:10.1007/s00383-017-4150-z
更新日期:2017-11-01 00:00:00
abstract::Our presentation of four cases demonstrates the essential features of limb-body wall complex (LBWC), representing a compound anomaly pattern in body-wall defects. The diagnosis of this entity is based on two of the three following characteristics: (1) exencephaly/encephalocele and facial clefts; (2) thoraco- and/or ab...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000497
更新日期:2001-07-01 00:00:00
abstract::A retrospective study of all cases (n = 21) of congenital lobar emphysema (CLE) treated at the Royal Hospital, Muscat, from September 1988 to August 1999 was performed. The presentation, diagnosis, treatment, and outcome are reviewed. All 21 patients were nonwhite. Thirteen had left-upper-lobe, 7 right-middle-lobe, an...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000506
更新日期:2001-03-01 00:00:00
abstract:OBJECTIVE:Postoperative anxiety symptoms are distressing for both family and child. The aim of this study was to examine the prevalence of postoperative anxiety symptoms in children. METHODS:60 children aged 6-12 undergoing surgery were included in the study group. The study group was assessed three times in terms of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4273-x
更新日期:2018-07-01 00:00:00
abstract::A case of an epigastric giant-cell fibroblastoma is reported in a 6-year-old girl who had undergone a bone-marrow transplant for severe combined immunodeficiency secondary to adenosine deaminase deficiency. A small subcutaneous nodule had been excised from the epigastrium at age 12 months. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0827-y
更新日期:2003-08-01 00:00:00
abstract::Hypospadias surgery is one of the most difficult areas in pediatric urology and has been characterized by constant evolution. Some of the surgical techniques proposed in the past are now considered inadequate because of an unacceptable complication rate or poor functional and aesthetic results. The key for assessing a...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1147-1
更新日期:2004-03-01 00:00:00
abstract:INTRODUCTION:No protocol exists for prophylaxis or therapeutic management of peripherally inserted central catheter (PICC) related deep venous thrombosis (DVT) in children. Currently, very few patients are provided prophylaxis for DVT. In this study, we analyzed our current practice of PICC placement to identify the fr...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3259-y
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:Although the precise pathogenesis of ventral body wall (VBW) defects is not clearly understood, it has recently postulated that disruption of somite development during early embryogenesis may cause failure of proper VBW formation. The administration of cadmium (Cd) after 60 h of incubation induces omphalocele s...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2994-1
更新日期:2012-02-01 00:00:00
abstract::A 31-year-old man who died of metastatic adenocarcinoma of the sigmoid colon had undergone bilateral ureterosigmoidostomy for exstrophy of the bladder 20 years earlier. The patient never underwent a colonoscopy. Neoplastic transformation at the ureterosigmoid anastomosis must be considered as a possible severe complic...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050458
更新日期:1998-11-01 00:00:00
abstract:PURPOSE:In 2006, The Japanese Society of Pediatric Endoscopic Surgeons devised a plan to develop a pediatric endoscopic surgical skill qualification (ESSQ) system. This system is controlled by The Japan Society for Endoscopic Surgery. The standard requirement for skills qualification is the ability of each applicant to...
journal_title:Pediatric surgery international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00383-011-2871-y
更新日期:2011-07-01 00:00:00
abstract:AIM:To share our experience in ovary-sparing surgery for teratomas in children. PATIENTS AND METHODS:The medical records of nine patients (mean age of 11.2 years, r 6-15 years) who had undergone ovary-sparing surgery for teratoma were analyzed retrospectively. Mean duration for follow-up was 29.5 months (r 15-75 month...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3228-x
更新日期:2013-03-01 00:00:00
abstract::Necrotizing fasciitis (NF) is a potentially life-threatening infection of soft tissues. It is characterized by rapid spread of inflammation and infection with widespread necrosis of fascia, subcutaneous tissues, and overlying skin. NF is usually reported in adults with preexisting medical conditions or compromised imm...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1481-y
更新日期:2005-08-01 00:00:00
abstract::Interposing vascularized flaps between urethra and skin sutures is recommended in hypospadias surgery to decrease the rate of urethrocutaneous fistula. For this purpose nine patients with failed results after at least one hypospadias surgery and with insufficient foreskin were included in the study. Tubularized incise...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-0989-2
更新日期:2003-11-01 00:00:00
abstract:PURPOSE:Intestinal perforation (IP) is a fatal complication in extremely low birth weight infants (ELBWI). We started administrating enteral miconazole (MCZ) to ELBWI in 2002. Since then, the incidence of IP has significantly decreased. The aim of this study was to elucidate the prophylactic effect of MCZ for the treat...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3946-6
更新日期:2016-10-01 00:00:00
abstract:AIM OF THE STUDY:Liver retraction during laparoscopic fundoplication is important for obtaining an optimal space. Several methods have been developed, but the risks and benefits are unclear. We compared three different approaches and evaluated their safety and utility. METHODS:Forty-three neurologically impaired patie...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04646-8
更新日期:2020-05-01 00:00:00
abstract::The aim of this study is to evaluate the safety and efficacy of the redo transanal endorectal pull-through (TEPT) for patients with persistent symptoms after pull-through for Hirschsprung's disease (HD). Seven children were included in the study, their ages ranged from 2.5 to 6 years (four males and three females). Th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1837-y
更新日期:2007-02-01 00:00:00
abstract:PURPOSE:The aim of this study was to determine the incidence of gastroschisis-related complications (GRCs) after closure of the abdominal wall defect, with a focus on frequency, type and timing of required surgical interventions, and to identify the impact of these on further outcome. METHODS:All gastroschisis patient...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3500-3
更新日期:2014-06-01 00:00:00
abstract:AIM:Prepubertal testicular tumors are rare in children. We aim to present clinical and histological features of prepubertal testicular tumors through the analysis of the long-term experiences of a single medical center of China. MATERIALS AND METHODS:A total of 67 children (≤ 14 years) treated for testicular tumor at ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4366-6
更新日期:2018-12-01 00:00:00
abstract::A 5-month-old female was referred to the paediatric surgery clinic with a neck swelling in the right supra-clavicular region. This was thought clinically to be cystic hygroma. Pathology showed an ectopic salivary gland. This should be added to the list of differential diagnoses of neck swelling in childhood. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2366-2
更新日期:2009-06-01 00:00:00
abstract::Minimally invasive surgery (MIS) for cancer patients has become widely accepted in general surgery, however, it has not completely replaced the standard open operative procedures in pediatric oncology. The aim of this study was to evaluate the host relationship following MIS in a murine model of retroperitoneal neurob...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050271
更新日期:1998-03-01 00:00:00