Abstract：
PURPOSE:Lgr5+ intestinal epithelial stem cells (ISCs) crucial for intestinal epithelial regeneration are impaired during necrotizing enterocolitis. This study aims to investigate the influence of different stressors on intestinal epithelial injury and regeneration in vitro. METHODS:Intestinal epithelial cells (IEC-18) were exposed to stressors such as lipopolysaccharide, hydrogen peroxide, and serum. Cell viability was assessed using MTT assay at 18 and 24 h. IL-6 and Lgr5 gene expressions were measured using qPCR. RESULTS:IEC-18 cell viability decreased 18 h following administration of lipopolysaccharide, hydrogen peroxide, and low serum concentration. However, after 24 h, the decrease in cell viability was observed only in higher, but not in lower concentrations of lipopolysaccharide and hydrogen peroxide. IL-6 expression increased in all groups compared to control. Lgr5 expression was up-regulated in cells exposed to a single stressor, but down-regulated when multiple stressors were administered. CONCLUSION:Lipopolysaccharide, hydrogen peroxide, or low serum induced IEC-18 injury. The upregulation of Lgr5 expression after exposure to a single stressor suggests that minor injury to IEC-18 induces Lgr5+ ISCs to stimulate repair. Conversely, when IEC-18 cells were exposed to multiple stressors, Lgr5 expression was reduced. We speculate that this finding is similar to what happens in NEC when multiple stressors cause impairment of intestinal epithelium regeneration.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Lee C,Minich A,Li B,Miyake H,Seo S,Pierro Adoi
10.1007/s00383-017-4183-3subject
Has Abstractpub_date
2018-02-01 00:00:00pages
155-160issue
2eissn
0179-0358issn
1437-9813pii
10.1007/s00383-017-4183-3journal_volume
34pub_type
杂志文章abstract：:For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830100602
更新日期：2001-05-01 00:00:00
abstract：:This study describes a subset of patients with posterior urethral valves (PUV) who presented late in childhood. The objective was to identify factors that lead to back-pressure effects on the upper tracts, which persist in spite of adequate valve ablation in some patients, and seek factors that may preserve the upper ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050384
更新日期：1998-09-01 00:00:00
abstract：:We present the case of a 14-year-old male with a history of small bowel transplantation for long segment Hirschsprung's disease who underwent Duhamel ileorectal pull-through procedure. In post-transplant, the patient had no restrictions and was not TPN-dependent. To improve his quality of life, he and his family were ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-017-4216-y
更新日期：2018-01-01 00:00:00
abstract：:The factors that affect survival in patients with stage 4 neuroblastoma vary. Several prospective and retrospective studies have provided conflicting conclusions regarding the benefit of combining aggressive chemotherapy with complete surgical resection. We analyzed our experience to evaluate the effect of complete su...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-004-1353-x
更新日期：2005-02-01 00:00:00
abstract：PURPOSE:Pediatric surgeons at our institution are often asked by families about a theoretical increased risk of severe common upper respiratory infections in children status post lung resection. No data exist on this topic. We, therefore, aimed to examine the risk of severe respiratory infection in children after pulmo...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-020-04851-5
更新日期：2021-01-10 00:00:00
abstract：:Hirschsprung disease (HSCR) is a fairly well understood congenital, genetically based functional obstruction due to the congenital absence of ganglion cells in the distal bowel. However, although over 90% of Hirschsprung cases conform to the normally accepted histological diagnostic criteria, it has become increasingl...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-016-4038-3
更新日期：2017-03-01 00:00:00
abstract：:Surgical extirpation is the treatment of choice for symptomatic mullerian duct remnants (prostatic utricle, PU), and several surgical approaches have been described for the treatment of this pathology. A group of 11 patients with symptomatic PU were observed and treated. Associated anomalies included proximal or penos...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：
更新日期：1997-03-21 00:00:00
abstract：:The intrinsic innervation of the developing gut has long been a subject of investigation, but little is known regarding that of the embryonic cloaca. The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make up a large part of ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-005-1590-7
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:Objectively evaluating the lack of bowel control (fecal incontinence) continues to be a challenge. Many have attempted to measure the severity of fecal incontinence and to evaluate its impact on the quality of life by developing standardized scoring systems. Some of these systems have been validated but no...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-016-3909-y
更新日期：2016-08-01 00:00:00
abstract：PURPOSE:Rapid assessment of volume status in children is often difficult. The purpose of this study was to evaluate the feasibility of surgeon-performed ultrasound to assess volume status in patients with hypertrophic pyloric stenosis. METHODS:Ultrasounds were performed on admission and before operation. The diameters...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-015-3798-5
更新日期：2015-12-01 00:00:00
abstract：PURPOSE:The purpose of this study was to review the clinical characteristics and prognosis of children with adrenocortical tumors (ACT). METHODS:We retrospectively reviewed the medical records of 28 patients with ACT at our hospital between March 2010 and March 2017. RESULTS:The main clinical presentations were sexua...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-018-4409-z
更新日期：2019-03-01 00:00:00
abstract：:70 cases of gastroschisis (GS) were surgically treated at the Pediatric Surgical University Clinic, Münster, from 1984 through 1998. The defect occurred more frequently in males (44) than females (26). The average birth weight was 2,383 g and mean gestational age 36.8 weeks. 9 infants (12.9%) were delivered vaginally ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-002-0799-y
更新日期：2002-09-01 00:00:00
abstract：:The management of a critical airway in infants and toddlers with congenital tracheal stenosis (CTS) continues to be an enormous challenge to the surgeon. Until recently, this condition often proved fatal. Improvements in surgical techniques, anesthetic management, and postoperative critical care have resulted in succe...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s003830050028
更新日期：2000-01-01 00:00:00
abstract：PURPOSE:Hirschsprung disease (HSCR) has previously been associated with increased mortality. The aim of this study was to assess mortality in patients with Hirschsprung disease in a population-based cohort. METHODS:This was a nationwide, population-based cohort study. The study exposure was HSCR and the study outcome ...
journal_title：Pediatric surgery international
pub_type： 杂志文章,多中心研究
doi：10.1007/s00383-017-4150-z
更新日期：2017-11-01 00:00:00
abstract：:Incarcerated inguinal hernias in infants are commonly encountered. Reduction with intravenous sedation using morphine and related drugs carries risks of apnoea and/or respiratory arrest, especially in small, ex-premature babies who have an increased incidence of incarceration and thus opiate use is best avoided. Cauda...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-005-1534-2
更新日期：2005-09-01 00:00:00
abstract：AIM:Although liver abscess (LA) is prevalent worldwide, especially in developing countries, there is a paucity of data regarding the protocol for management of LA in children. The aim of this study was to analyze the outcome of a protocol-based management of pediatric LA from a single institution. MATERIALS AND METHOD...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-016-4009-8
更新日期：2017-02-01 00:00:00
abstract：:Numerous case reports in the literature have suggested a causal link between beta-haemolytic streptococcal infection and delayed-onset right-sided diaphragmatic hernia/eventration in the perinatal age group. We report a child with antenatally documented right diaphragmatic eventration 4 weeks before birth who subseque...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：
更新日期：1997-03-21 00:00:00
abstract：:The transverse colon is an exceptional location of intestinal duplication. Perforated duplications are rarely described in neonates. Meconium peritonitis (MP) can originate from prenatal perforated intestinal duplication. The authors report a case of a baby girl with prenatal diagnosis of MP. Rapid worsening of clinic...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-004-1349-6
更新日期：2005-02-01 00:00:00
abstract：:Abdominal actinomycosis (AMC) is a rare infection in children. The appendix is the most common intra-abdominal organ involved. It presents as an undifferentiated mass, forming abscesses and fistulas in the right lower quadrant. The case of a 15-year-old girl with a AMC of the appendix detected by the pathologist after...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050515
更新日期：1999-01-01 00:00:00
abstract：:Non-accidental trauma (NAT) represents a significant cause of morbidity and mortality in the pediatric population. The management of these patients often involves many care providers including the surgeon. Victims of NAT often present with multiple injuries and as such should be treated as trauma patients with complet...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-015-3688-x
更新日期：2015-07-01 00:00:00
abstract：:To assess the important factors for successful primary closure in staged reconstruction of bladder exstrophy, 25 patients (18 males, 7 females) underwent primary bladder closure during the years 1993-1997. Twenty-one were more than 72 h old; all of these underwent bilateral posterior iliac osteotomies followed by prim...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050721
更新日期：2000-01-01 00:00:00
abstract：:Our presentation of four cases demonstrates the essential features of limb-body wall complex (LBWC), representing a compound anomaly pattern in body-wall defects. The diagnosis of this entity is based on two of the three following characteristics: (1) exencephaly/encephalocele and facial clefts; (2) thoraco- and/or ab...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830000497
更新日期：2001-07-01 00:00:00
abstract：:We report a rare case of massive haematochezia due to hookworm infestation that required two laparotomies before the cause of bleeding could be diagnosed. The need to consider this parasite as a possible cause of gut bleeding in endemic countries is stressed, as early recognition may avoid unnecessary investigations a...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/BF00497827
更新日期：1996-06-01 00:00:00
abstract：:The most common cystic lesion recognized antenatally is multicystic dysplastic kidney (MCDK). Recently, conservative management without nephrectomy has been advocated. The purpose of this study was to report our experience in the conservative management of unilateral MCDK. Between 1989 and 1997, 20 children with MCDK ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830000449
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:Controversy exists as regards the best non-invasive diagnostic tool for pediatric cervical lymphadenopathy. The current work aimed to evaluate the reliability, sensitivity, specificity, and accuracy of sonoelastography in diagnosing benign and/or malignant pediatric cervical lymphadenopathy. METHODS:Prospectiv...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-018-4301-x
更新日期：2018-08-01 00:00:00
abstract：UNLABELLED:Anorectal malformation (ARM) is a group of significant birth defects with geographic variation in incidence, individual phenotypes and regional geographic subtypes occurring in approximately 1:5,000 live births. Anorectal atresia with gross terminal colonic distension in the presence of an absent anal canal ...
journal_title：Pediatric surgery international
pub_type： 杂志文章,多中心研究
doi：10.1007/s00383-013-3346-0
更新日期：2013-12-01 00:00:00
abstract：BACKGROUND/PURPOSE:To determine the frequency and characteristics of suspected cow's milk protein allergy (CMPA) in infants with gastroschisis and response to change in milk. METHODS:A retrospective cohort study of 111 consecutive infants with gastroschisis. RESULTS:64 episodes suggesting non-IgE-mediated CMPA occurr...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-015-3664-5
更新日期：2015-03-01 00:00:00
abstract：:Our case report details the clinical course and management of a neonate with acute life-threatening respiratory distress presenting soon after birth. She needed intubation and ventilation. A pericardial cyst was diagnosed after a CT scan and 2D echo investigation. As a temporizing procedure the fluid was tapped and th...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-003-0970-0
更新日期：2003-11-01 00:00:00
abstract：PURPOSE:The use of general anesthesia in young children has come under increasing scrutiny due to its potential long-term neurotoxic effects. Meanwhile, regional anesthesia for surgical procedures in neonates has many advantages, including preservation of respiratory status and faster return to feeding. We describe the...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-016-4027-6
更新日期：2017-03-01 00:00:00
abstract：:The diagnosis of right-sided diaphragmatic rupture (RDR) due to blunt abdominal trauma is often missed in the acute setting, especially in the absence of other thoracoabdominal injuries. We describe two such children. The problems associated with the diagnosis and management of RDR are discussed to emphasize the need ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830100686
更新日期：2002-03-01 00:00:00
