当前位置： SCI文献检索 > JOURNAL OF PLANT PHYSIOLOGY期刊下所有文献 > Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.

Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.

Abstract：

:We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomatal conductance and a slight retardation of leaf growth, but the osmotic potential remained constant. Using the differential display technique, we searched for genes differentially expressed in response to drought at this date. Thirty-six differentially expressed leaf cDNAs were detected between stressed and control conditions. Thirty-four cDNAs clones were successfully cloned and 23 were found to share high identity with Arabidopsis thaliana and Populus trichocarpa genes. The transcriptional regulation of 21 genes was examined by reverse RNA dot blot, confirming an increase in expression for 16 of them after 10 days of treatment. Among these 16 genes, most of them are involved in a different cellular metabolic pathway. These differentially expressed genes are also involved and/or regulated by other treatments such as salt, withholding water or auxin application. The maintenance of growth observed during the first 10 days of the stress period could be due to the regulation of these genes and can be a common response between herbaceous plants and trees.

journal_name

J Plant Physiol

journal_title

Journal of plant physiology

authors

Caruso A,Chefdor F,Carpin S,Depierreux C,Delmotte FM,Kahlem G,Morabito D

doi

10.1016/j.jplph.2007.04.006

subject

Has Abstract

pub_date

2008-06-16 00:00:00

pages

932-41

issue

9

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(07)00219-2

journal_volume

165

pub_type

杂志文章

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