Abstract:
:Cysteine synthesis in plants represents the final step of assimilatory sulfate reduction and the almost exclusive entry reaction of reduced sulfur into metabolism not only of plants, but also the human food chain in general. It is accomplished by the sequential reaction of two enzymes, serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL). Together they form the hetero-oligomeric cysteine synthase complex (CSC). Recent evidence is reviewed that identifies the dual function of the CSC as a sensor and as part of a regulatory circuit that controls cellular sulfur homeostasis. Computational modeling of three-dimensional structures of plant SAT and OAS-TL based on the crystal structure of the corresponding bacterial enzymes supports quaternary conformations of SAT as a dimer of trimers and OAS-TL as a homodimer. These findings suggest an overall alpha6beta4 structure of the subunits of the plant CSC. Kinetic measurements of CSC dissociation triggered by the reaction intermediate O-acetylserine as well as CSC stabilization by sulfide indicate quantitative reactions that are suited to fine-tune the equilibrium between free and associated CSC subunits. In addition, in vitro data show that SAT requires binding to OAS-TL for full activity, while at the same time bound OAS-TL becomes inactivated. Since OAS concentrations inside cells increase upon sulfate deficiency, whereas sulfide concentrations most likely decrease, these data suggest the dissociation of the CSC in vivo, accompanied by inactivation of SAT and activation of OAS-TL function in their free homo-oligomer states. Biochemical evidence describes this protein-interaction based mechanism as reversible, thus closing the regulatory circuit. The properties of the CSC and its subunits are therefore consistent with models of positive regulation of sulfate uptake and reduction in plants by OAS as well as a demand-driven repression/de-repression by a sulfur intermediate, such as sulfide.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Wirtz M,Hell Rdoi
10.1016/j.jplph.2005.11.013subject
Has Abstractpub_date
2006-02-01 00:00:00pages
273-86issue
3eissn
0176-1617issn
1618-1328pii
S0176-1617(05)00461-Xjournal_volume
163pub_type
杂志文章,评审abstract::The cherry rootstock 'Colt' line was transformed with a phytochrome A rice gene with the aim of altering light perception. Three transgenic events were chosen because of a modified developmental behavior. When red enriched light was supplied horizontally to stems, the PD3 transgenic line showed an increased rate of ph...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.01.009
更新日期:2008-07-07 00:00:00
abstract::2,4-Epibrassinolide (EBL) is a plant hormone that plays a pivotal role in regulation of plants growth and development processes under abiotic stress. The investigation was carried out to study the effect of EBL on mineral nutrients uptake and distribution with ion element analysis and X-ray microanalysis in cucumber s...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.06.010
更新日期:2015-09-01 00:00:00
abstract::Autophagy, a highly conserved intracellular degradation system, is regarded to be responsible for self-defense and protect cells from abiotic stress. Extensive studies have demonstrated that autophagy plays a crucial role in regulating plant growth and development as well as in response to diverse stresses. However, l...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.06.012
更新日期:2018-10-01 00:00:00
abstract::A sand culture experiment was conducted to answer the question whether or not exogenous KNO(3) can alleviate adverse effects of salt stress in winter wheat by monitoring plant growth, K(+)/Na(+) accumulation and the activity of some antioxidant enzymes. Seeds of two wheat cultivars (CVs), DK961 (salt-tolerant) and JN1...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.01.001
更新日期:2008-09-29 00:00:00
abstract::Recent data indicate the existence of at least three L-ascorbic acid (AsA) biosynthetic pathways in plant cells. Studying their occurrence in different plant organs and species may help to decipher the precise role(s) of AsA in plant cell physiology. In grape berries, AsA is of particular importance since it is known ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.017
更新日期:2010-06-15 00:00:00
abstract::The phytohormone auxin is involved in the regulation of a variety of developmental processes. In this report, we describe how the processes of lateral root and root hair formations and root gravity response in rice are controlled by auxin. We use a rice mutant aem1 (auxin efflux mutant) because the mutant is defective...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.09.012
更新日期:2005-06-01 00:00:00
abstract::The uptake of liposomal contents into Catharanthus roseus protoplasts has been quantitated. Hi.phest uptake was obtained from positively charged vesicles (1.09% of vesicle contents per 10(7) protoplasts) in the presence of 10% w/v polyethylene glycol 4000. Significant uptake was also observed from negatively charged v...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80014-0
更新日期:1984-11-01 00:00:00
abstract::Cell suspension cultures of Silybum marianum are able to excrete silymarin compounds into the medium upon elicitation with methyl jasmonate or cyclodextrins. Knowledge of transport mechanism is important to understand Sm metabolism and to develop strategies aimed at increasing production by means of cell cultures. For...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.10.005
更新日期:2014-01-15 00:00:00
abstract::Arbuscular mycorrhizal fungal (AMF) colonization can induce both the local and the systemic increase in phenolic accumulation in hosts. However, the signaling molecules responsible for the systemic induction is still unclear. In this study, a split-root rhizobox system was designed to explore these molecules, with one...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.01.016
更新日期:2015-04-15 00:00:00
abstract::The effects of different doses of NaCl on the expression profiles of genes involved in the mitochondrial electron transport chain (miETC), H2O2 and O2- levels, and antioxidant enzymes and amino acid metabolism were investigated in the leaves of poplar (Populus deltoides x Populus euramericana 'Nanlin 895'). In the miE...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153017
更新日期:2019-09-01 00:00:00
abstract::Molecular chaperones perform folding assistance in newly synthesized polypeptides preventing aggregation processes, recovering proteins from aggregates, among other important cellular functions. Thus their study presents great biotechnological importance. The present work discusses the mining for chaperone-related seq...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.03.013
更新日期:2007-04-01 00:00:00
abstract::Some intracellular organelles found in eukaryotes such as plants have arisen through the endocytotic engulfment of prokaryotic cells. This accounts for the presence of plant membrane intrinsic proteins that have homologs in prokaryotic cells. Other organelles, such as those of the endomembrane system, are thought to h...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.09.022
更新日期:2014-05-15 00:00:00
abstract::A typical symptom of postharvest chilling injury (PCI) in pineapple fruit (Ananas comosus (L.) Merr.) is internal browning (IB) near the fruit core. Since vascular bundles (VBs) are localized to this region, it was hypothesized that the VBs might be the site of IB. To test this, the anatomy and histochemistry of VBs d...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.07.011
更新日期:2016-09-01 00:00:00
abstract::Using a direct somatic embryogenesis system in carrot, we examined the role of DNA methylation in the change of cellular differentiation state, from somatic to embryogenic. 5-Azacytidine (aza-C), an inhibitor of DNA methylation suppressed the formation of embryogenic cell clumps from epidermal carrot cells. Aza-C also...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.05.013
更新日期:2005-01-01 00:00:00
abstract::In order to test an alternative selectable marker system for the production of transgenic peanut plants (Arachis hypogaea), the bacterial mercuric ion reductase gene, merA, was introduced into embryogenic cultures via microprojectile bombardment. MerA reduces toxic Hg(II) to the volatile and less toxic metallic mercur...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01087
更新日期:2003-08-01 00:00:00
abstract::The effect of excess copper on the expression of soluble proteins in 10-day old Phaseolus vulgaris seedlings was studied with two-dimensional electrophoresis and mass spectrometry, to find sensitive biochemical markers of exposure. Despite major differences in root Cu contents, both 15 and 50 microM Cu treatments resu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.07.018
更新日期:2005-04-01 00:00:00
abstract::In prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. Here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.08.005
更新日期:2007-10-01 00:00:00
abstract::Calcium plays a crucial role as a secondary messenger in all aspects of plant growth, development and survival. Calcium dependent protein kinases (CDPKs) are the major calcium decoders, which couple the changes in calcium level to an appropriate physiological response. The mechanism by which calcium regulates CDPK pro...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.03.005
更新日期:2015-05-01 00:00:00
abstract::A fragment encoding a partial sequence of a prohibitin (Phb) gene was isolated. The expression of Phb mRNA and protein in seeds of wild type and mutant Arabidopsis thaliana is presented. Phb mRNA is abundant in wild-type seeds; thus, it may have sequence or structural characteristics responsible for this stability. Th...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.05.002
更新日期:2007-03-01 00:00:00
abstract::Researchers have proven that nanomaterials have a significant effect on plant growth and development. To better understand the effects of nanomaterials on plants, Zhongshuang 11 was treated with different concentrations of graphene oxide. The results indicated that 25-100mg/l graphene oxide treatment resulted in short...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.02.011
更新日期:2016-04-01 00:00:00
abstract::The ultrastructure of mesophyll cells was studied in leaves of the Triticum aestivum L. cv. "Trizo" seedlings after two weeks of growth on soil contaminated by Pb and/or Se. The soil treatments: control; (Pb1) 50mgkg-1; (Pb2) 100mgkg-1; (Se1) 0.4mgkg-1; (Se2) 0.8mgkg-1; (Pb1+Se1); (Pb1+Se2); (P2+Se1); and (Pb2+Se2) we...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.008
更新日期:2017-12-01 00:00:00
abstract::Chlorophyll b (Chlb) is an antenna chlorophyll. The binding of Chlb by antenna proteins is crucial for the correct assembly of the antenna complexes in thylakoid membranes. Since the levels of the proteins of major and minor antenna are affected to different extents by Chlb binding, the availability of Chlb influences...
journal_title:Journal of plant physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jplph.2015.09.013
更新日期:2015-09-15 00:00:00
abstract::Rhizoctonia solani is a fungal pathogen that causes sheath blight disease in rice plants. In this study, metabolomic analysis using CE/TOF-MS in negative ion mode was used to investigate the resistance response of resistant and susceptible rice lines (32R and 29S, respectively) due to R. solani infection. Two rice lin...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.09.004
更新日期:2016-11-01 00:00:00
abstract::Low temperature is one of the primary causes of economic loss in agricultural production, and in this regard, expansin proteins are known to play important roles in plant growth and responses to various abiotic stresses and plant hormones. In order to elucidate the roles of expansin genes in the response of Dongnongdo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153004
更新日期:2019-09-01 00:00:00
abstract::Plant viral infections alter gene expression and metabolism in infected host. To study the molecular responses of Mexican lime to CTV infection, an analysis of plant metabolome in response to infection with severe (T318) or mild (T385) isolates of CTV was performed. Healthy plants and those infected with any of the tw...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.05.001
更新日期:2019-07-01 00:00:00
abstract::Ascorbate is a major antioxidant and radical scavenger in plants. Monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR) are two enzymes of the ascorbate-glutathione cycle that maintain ascorbate in its reduced state. MDAR2 (At3g09940) and DHAR5 (At1g19570) expression was upregulated in the roots ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.12.016
更新日期:2009-08-15 00:00:00
abstract::Oxidative stress and ultrastructural changes under hexavalent chromium stress were investigated in developing rice seedlings. Chromium treatment for 24 or 48h resulted in inhibition of root length and dry biomass. Atomic absorption spectrometry analysis of roots showed that chromium accumulation increased with increas...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.01.012
更新日期:2007-11-01 00:00:00
abstract::Hyacinth (Hyacinthus orientalis) bulbs infected by Fusarium oxysporum showed the symptoms of gummosis. The purpose of this study was to clarify the hormonal regulation of gummosis and composition of gums from hyacinth bulbs. The application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2014.10.007
更新日期:2015-02-01 00:00:00
abstract::Cinnamoyl CoA reductase catalyzes the reduction of cinnamic acid CoA esters into their corresponding aldehydes, the first step of the phenylpropanoid pathway specifically dedicated to monolignol biosynthesis. Two cDNA clones encoding cinnamoyl CoA reductase (CCR) have been isolated from Hordeum vulgare (HvCCR) and Sol...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01074
更新日期:2004-01-01 00:00:00
abstract::Plants developed receptors for solar UV-A/B radiation, which regulate a complex network of functions through the plant's life cycle. However, greenhouse grown crops, like tomato, are exposed to strongly reduced UV radiation, contrarily to their open-field counterparts. A new paradigm of modern horticulture is to suppl...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.11.013
更新日期:2018-02-01 00:00:00