Abstract：
:In order to test an alternative selectable marker system for the production of transgenic peanut plants (Arachis hypogaea), the bacterial mercuric ion reductase gene, merA, was introduced into embryogenic cultures via microprojectile bombardment. MerA reduces toxic Hg(II) to the volatile and less toxic metallic mercury molecule, Hg(0), and renders its source Gram-negative bacterium mercury resistant. A codon-modified version of the merA gene, MerApe9, was cloned into a plant expression cassette containing the ACT2 promoter from Arabidopsis thaliana and the NOS terminator. The expression cassette also was inserted into a second vector containing the hygromycin resistance gene driven by the UBI3 promoter from potato. Stable transgenic plants were recovered through hygromycin-based selection from somatic embryo tissues bombarded with the plasmid containing both genes. However, no transgenic somatic embryos were recovered from selection on 50-100 micromol/L HgCl2. Expression of merA as mRNA was detected by Northern blot analysis in leaf tissues of transgenic peanut, but not in somatic embryos. Western blot analysis showed the production of the mercuric ion reductase protein in leaf tissues. Differential responses to HgCl2 of embryo-derived explants from segregating R1 seeds of one transgenic line also were observed.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Yang H,Nairn J,Ozias-Akins Pdoi
10.1078/0176-1617-01087subject
Has Abstractpub_date
2003-08-01 00:00:00pages
945-52issue
8eissn
0176-1617issn
1618-1328journal_volume
160pub_type
杂志文章abstract：:Plants counteract Cd toxicity by activating cellular stress responses. The simultaneous exogenous application of methyl jasmonate (MeJA) and methyl-β-cyclodextrin (CD) before Cd exposure improved the response of Arachis hypogaea hairy root culture to the unfavourable effects of Cd toxicity. At 24 h after elicitation, ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2020.153278
更新日期：2020-11-01 00:00:00
abstract：:Oxidative stress and ultrastructural changes under hexavalent chromium stress were investigated in developing rice seedlings. Chromium treatment for 24 or 48h resulted in inhibition of root length and dry biomass. Atomic absorption spectrometry analysis of roots showed that chromium accumulation increased with increas...
journal_title：Journal of plant physiology
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更新日期：2007-11-01 00:00:00
abstract：:Suppression of photorespiration by low O2 concentrations (Method 1) and simultaneous measurements of gas exchange and chlorophyll fluorescence (Method 2) are often used to estimate leaf photorespiration rate (Rp) of C3 plants. However, it is largely unknown whether Method 1 and Method 2 can be used equivalently in est...
journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
abstract：:Aquaporins mediate the movement of water across cell membranes. Plasma membrane intrinsic protein 2;5 from Populus trichocarpa×deltoides (PtdPIP2;5) was previously demonstrated to be a functionally important water conducting aquaporin. To study the relevance of aquaporin-mediated root water transport at low temperatur...
journal_title：Journal of plant physiology
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更新日期：2016-03-15 00:00:00
abstract：:Gibberellin 20-oxidases, enzymes of gibberellin (GA) biosynthesis, play an important role in (GA) homeostasis. To investigate the regulation of tomato SlGA20ox1 expression, a genomic clone was isolated, its promoter transcriptionally fused to the GUS reporter gene, and the construct used to transform Arabidopsis. Expr...
journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2010-09-15 00:00:00
abstract：:The molybdenum cofactor (Moco) is ubiquitously present in all kingdoms of life and vitally important for survival. Among animals, loss of the Moco-containing enzyme (Mo-enzyme) sulphite oxidase is lethal, while for plants the loss of nitrate reductase prohibits nitrogen assimilation. Moco is highly oxygen-sensitive, w...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.10.002
更新日期：2016-12-01 00:00:00
abstract：:Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To identify these proteins in more detail, the bands with RNase activity...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.10.002
更新日期：2011-05-01 00:00:00
abstract：:Xanthomonas albilineans is the causal organism of leaf scald, a bacterial vascular disease of sugarcane. Xanthomonas may invade the parenchyma between the bundles and cause reddened pockets of gum, identified as a xanthan-like polysaccharide. Since xanthan contains glucuronic acid, the ability of Xanthomonas to produc...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.03.008
更新日期：2008-03-13 00:00:00
abstract：:An essential step in the biosynthesis of bioactive brassinosteroids (BRs) in plants is the hydroxylation at C-22, a reaction catalyzed by P450 enzymes of the CYP90B and CYP724B subfamilies. Genes for both types of enzymes are present in many species, and in rice (Oryza sativa) and tomato (Solanum lycopersicum) both CY...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.10.013
更新日期：2012-03-01 00:00:00
abstract：:The purpose of this paper is to demonstrate the complementary utility of chlorophyll fast fluorescence OJIP transient (from 50 microns to 1s) measurements in the aerial study of rows of trees. We identify limitations in photochemical events induced by urban injuries on Platanus acerfolia L. using the JIP-test procedur...
journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
abstract：:Strigolactones (SLs) or closely related molecules were recently identified as phytohormones, acting as long-distance branching factors that suppress growth of pre-formed axillary buds in the shoot. The SL signaling pathways and light appear to be connected, as SLs were shown to induce light-regulated pathways and to m...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.05.022
更新日期：2011-11-01 00:00:00
abstract：:The phytohormone auxin is involved in the regulation of a variety of developmental processes. In this report, we describe how the processes of lateral root and root hair formations and root gravity response in rice are controlled by auxin. We use a rice mutant aem1 (auxin efflux mutant) because the mutant is defective...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.09.012
更新日期：2005-06-01 00:00:00
abstract：:The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-spe...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.04.018
更新日期：2005-11-01 00:00:00
abstract：:Cryptochromes are blue-light photoreceptors that control many aspects of plant development. In this study, cryptochrome mutants of Arabidopsis were examined to assess the role of cryptchrome-1 (CRY1) in lateral roots growth. When grown in blue light for 12d, mutant seedlings (cry1) showed increased growth of lateral r...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.12.003
更新日期：2010-05-15 00:00:00
abstract：:Euglena gracilis, a unicellular photosynthetic flagellate, uses light and gravity as environmental hints to reach and stay in regions optimal for growth and reproduction. The current model of gravitaxis (the orientation with respect to the earth's gravitational field) is based on the specific density difference betwee...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-00194
更新日期：2001-01-01 00:00:00
abstract：:It has been shown that oxidative damage contributes to the wide range of toxic effects of the mycotoxin ochratoxin A (OTA). Therefore, we examined the effects of alpha-tocopherol (alpha-TOC) and different polyphenols--catechin (CAT), daidzein (DAI), epicatechin (EC), epigallocatechin gallate (EGCG), genistein (GEN), a...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.04.021
更新日期：2005-07-01 00:00:00
abstract：:The Southern Ocean (SO) is a major sink for anthropogenic atmospheric carbon dioxide (CO2), potentially harbouring even greater potential for additional sequestration of CO2 through enhanced phytoplankton productivity. In the SO, primary productivity is primarily driven by bottom up processes (physical and chemical co...
journal_title：Journal of plant physiology
pub_type： 杂志文章,评审
doi：10.1016/j.jplph.2016.05.004
更新日期：2016-09-20 00:00:00
abstract：:Potassium channels are important for many physiological functions in plants, one of which is to regulate plant adaption to stress conditions. In this study, KCT2, the gene encoding a membrane-bound protein potassium channel (GenBank accession number: ), was isolated from Chinese cabbage (Brassica rapa ssp. Pekinensis)...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.09.002
更新日期：2006-09-01 00:00:00
abstract：:Apocarotenoids modulate vital physiological and developmental processes in plants. These molecules are formed by the cleavage of carotenoids, a reaction catalyzed by a family of enzymes called carotenoid cleavage dioxygenases (CCDs). Apocarotenoids like β-ionone and β-cyclocitral have been reported to act as stress si...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.11.001
更新日期：2015-09-15 00:00:00
abstract：:Some intracellular organelles found in eukaryotes such as plants have arisen through the endocytotic engulfment of prokaryotic cells. This accounts for the presence of plant membrane intrinsic proteins that have homologs in prokaryotic cells. Other organelles, such as those of the endomembrane system, are thought to h...
journal_title：Journal of plant physiology
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doi：10.1016/j.jplph.2013.09.022
更新日期：2014-05-15 00:00:00
abstract：:Previous studies have shown that plants developed under high relative air humidity (RH>85%) develop malfunctioning stomata and therefor have increased transpiration and reduced desiccation tolerance when transferred to lower RH conditions and darkness. In this study, plants developed at high RH were exposed to daily V...
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doi：10.1016/j.jplph.2016.12.011
更新日期：2017-04-01 00:00:00
abstract：:Ascorbic acid oxidase (AAO) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. In spite of extensive molecular characterization the physiological role of AAO is still uncertain. Abundant mRNA, protein and activity of AAO were observed in illuminated leaves ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.09.016
更新日期：2007-01-01 00:00:00
abstract：:Alterations in temperature adaptation processes and changes in the content of stress-related compounds, polyamines and salicylic acid were evaluated in Atnoa1 (NO-associated 1) Arabidopsis mutant. The F(v)/F(m) chlorophyll-a fluorescence induction parameter and the actual quantum yield were significantly lower in the ...
journal_title：Journal of plant physiology
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doi：10.1016/j.jplph.2011.01.021
更新日期：2011-07-15 00:00:00
abstract：:Fruit development is a process involving various signals and gene expression. Protein phosphorylation catalyzed by protein kinases is known to play a key role in eukaryotic cell signalling and so may be involved in the regulation of fruit development. Using the method of exogenous substrate phosphorylation, we charact...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01038
更新日期：2004-01-01 00:00:00
abstract：:The flavor and taste of fruits are often determined by terpenes. We identified three cDNAs encoding putative terpene synthases from olive fruits of cv. Frantoio and Grignano. Heterologous expression in a bacterial system demonstrated that one of the terpene synthases, OeGES1, was an active monoterpene synthase that co...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.01.021
更新日期：2012-06-15 00:00:00
abstract：:Agronomic performance of transgenic tomato overexpressing functional genes has rarely been investigated in the field. In an attempt to improve low-phosphate (P) stress tolerance of tomato (Solanum lycopersicum) plants and promote tomato fruit production in the field, an expression vector containing cDNA to an Arabidop...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.04.005
更新日期：2018-07-01 00:00:00
abstract：:Bolting of lettuce is promoted by high temperatures. Gibberellins (GAs) play an important role in the bolting of several plant species, and it has been reported that exogenous GAs induce bolting and early flowering in lettuce. To clarify the role of GAs in this process, we examined the expression of genes involved in ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.06.003
更新日期：2009-12-15 00:00:00
abstract：:Paeonia ostii var. lishizhenii, a well-known medicinal and horticultural plant, is indigenous to China. Recent studies have shown that its seed has a high oil content, and it was approved as a novel resource of edible oil with a high level of α-linolenic acid by the Chinese Government. This study measured the seed oil...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.05.011
更新日期：2018-09-01 00:00:00
abstract：:Plant WRKY transcription factors play a vital role in abiotic stress tolerance and regulation of plant defense responses. This study examined AtWRKY11 and AtWRKY17 expression under ABA, salt, and osmotic stress at different developmental stages in Arabidopsis. We used reverse transcriptase PCR, quantitative real-time ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.04.007
更新日期：2018-07-01 00:00:00
abstract：:Common bean, one of the most important legumes for human consumption, may have drastic reduction in yield due to anthracnose, a disease caused by the fungus Colletotrichum lindemuthianum. Rapid induction of the plant defense mechanisms is essential to establish an incompatible interaction with this pathogenic fungus. ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.04.003
更新日期：2012-08-15 00:00:00
