HPLC screening of natural vitamin E from mediterranean plant biofactories--a basic tool for pilot-scale bioreactors production of alpha-tocopherol.


:The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-speed milling with Waring blender for 40 s. alpha-Tocopherol was extracted from the nut tissue powder using dehydrated hexane fortified with 0.01% butylated hydroxytoluene (BHT, co-antioxidant). The rate of alpha-tocopherol accumulation showed differences among nut samples collected in different areas of Italy. Sarda Piccola nut biofactory contained higher amount (81.17 microg/g d.w) of alpha-tocopherol than other-local eleven Italian cultivar nuts. These results provide insight into the biofactory basis for alpha-tocopherol accumulation in hazelnut and give the suitable cultivar tissues to establish pilot-scale bioreactors production of natural bioactive vitamin E.


J Plant Physiol


Sivakumar G,Bacchetta L,Gatti R,Zappa G




Has Abstract


2005-11-01 00:00:00












  • Developing embryos of Sesbania sesban have unique potential to photosynthesize under high osmotic environment.

    abstract::This study has been carried out to investigate the photosynthetic activities in developing embryos of Sesbania sesban under a highly osmotic environment. In S. sesban, the embryo turns green/chlorophyllous at the early heart shape stage. Interestingly, despite being deeply embedded within the supporting tissues (sever...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Puthur JT,Saradhi PP

    更新日期:2004-10-01 00:00:00

  • AtFtsH heterocomplex-mediated degradation of apoproteins of the major light harvesting complex of photosystem II (LHCII) in response to stresses.

    abstract::Chloroplastic heterocomplex consisting of AtFtsH1, 2, 5 and 8 proteases, integrally bound to thylakoid membrane was shown to play a critical role in degradation of photodamaged PsbA molecules, inherent to photosystem II (PSII) repair cycle and in plastid development. As no one thylakoid bound apoproteins besides PsbA ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Luciński R,Jackowski G

    更新日期:2013-08-15 00:00:00

  • Light is a positive regulator of strigolactone levels in tomato roots.

    abstract::Strigolactones (SLs) or closely related molecules were recently identified as phytohormones, acting as long-distance branching factors that suppress growth of pre-formed axillary buds in the shoot. The SL signaling pathways and light appear to be connected, as SLs were shown to induce light-regulated pathways and to m...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Koltai H,Cohen M,Chesin O,Mayzlish-Gati E,Bécard G,Puech V,Ben Dor B,Resnick N,Wininger S,Kapulnik Y

    更新日期:2011-11-01 00:00:00

  • Chlorophyll b in angiosperms: Functions in photosynthesis, signaling and ontogenetic regulation.

    abstract::Chlorophyll b (Chlb) is an antenna chlorophyll. The binding of Chlb by antenna proteins is crucial for the correct assembly of the antenna complexes in thylakoid membranes. Since the levels of the proteins of major and minor antenna are affected to different extents by Chlb binding, the availability of Chlb influences...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章,评审


    authors: Voitsekhovskaja OV,Tyutereva EV

    更新日期:2015-09-15 00:00:00

  • Expression profiling of two stress-inducible genes encoding for miraculin-like proteins in citrus plants under insect infestation or salinity stress.

    abstract::The expression of two genes, namely Mir1 and Mir3 and the abundance of their encoded proteins, the putative miraculin-like proteins, MLP1 and MLP3, showing similarity to the Kunitz family of protease inhibitors, were monitored in the leaves of the citrus variety, 'Clementine' after Tetranychus urticae infestation and ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Podda A,Simili M,Del Carratore R,Mouhaya W,Morillon R,Maserti BE

    更新日期:2014-01-01 00:00:00

  • Half-life of tRNA and the Initial Rate of tRNA Accumulation in Plant Cells from Cell Suspension Cultures of Lupinus angustifolius cv. Turkus and Petroselinum hortense cv. Berlińska Growing at Different Rates.

    abstract::Half-lives of tRNA and the initial rates of tRNA accumulation are correlated with the growth rates of cell suspension cultures of Lupinus angustifolius cv. Turkus and Petroselinum hortense cv. Berlinska. Half-lives of tRNA are approximately proportional to the doubling times of cells in these cultures. The initial rat...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Sroga GE

    更新日期:1984-08-01 00:00:00

  • Actin microfilaments are involved in the regulation of HVA1 transcript accumulation in drought-treated barley leaves.

    abstract::Drought is one of the stresses that limit the yield of barley. Despite extensive studies focused on the issue, the molecular mechanism of the response to drought is still not fully understood. In our previous study, we proposed drought-induced signal perception controlled by actin filaments (AFs). To test this hypothe...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Śniegowska-Świerk K,Dubas E,Rapacz M

    更新日期:2016-04-01 00:00:00

  • Anthocyanin accumulation is initiated by abscisic acid to enhance fruit color during fig (Ficus carica L.) ripening.

    abstract::Fig fruit is well-known for its attractive flavor, color, and nutritional and medicinal value. Anthocyanin contributes to the fruit's color and constitutes a high percentage of the total antioxidant content of the fig fruit. We quantified the major anthocyanins and characterized the expression levels of anthocyanin-bi...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Lama K,Harlev G,Shafran H,Peer R,Flaishman MA

    更新日期:2020-08-01 00:00:00

  • Photochemical processes, carbon assimilation and RNA accumulation of sucrose transporter genes in tomato arbuscular mycorrhiza.

    abstract::Arbuscular mycorrhizal fungi enhance CO₂ assimilation of their hosts which ensure the demand for carbohydrates of these obligate biotrophic microorganisms. Photosynthetic parameters were measured in tomato colonised or not by the arbuscular mycorrhizal fungus Glomus mosseae. In addition, carbohydrate contents and mRNA...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Boldt K,Pörs Y,Haupt B,Bitterlich M,Kühn C,Grimm B,Franken P

    更新日期:2011-07-15 00:00:00

  • Changes in the ascorbate system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress.

    abstract::The involvement of the ascorbate (AsA) system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress was studied. The treatment of 5-day-old pumpkin seedlings with 50 microM aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumul...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Dipierro N,Mondelli D,Paciolla C,Brunetti G,Dipierro S

    更新日期:2005-05-01 00:00:00

  • Comparing contributions of soil versus root colonization to variations in stomatal behavior and soil drying in mycorrhizal Sorghum bicolor and Cucurbita pepo.

    abstract::In prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. Here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Augé RM,Toler HD,Moore JL,Cho K,Saxton AM

    更新日期:2007-10-01 00:00:00

  • Glucose-induced inhibition of seed germination in Lotus japonicus is alleviated by nitric oxide and spermine.

    abstract::Seed germination is sensitive to glucose (Glc), nitric oxide (NO) and polyamine (PA). To elucidate whether cross-talk among Glc, NO and PAs occurs in mediation of seed germination, effects of Glc, NO and spermine on seed germination of Lotus japonicus were studied. Glc retarded seed germination in a concentration-depe...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Zhao MG,Liu RJ,Chen L,Tian QY,Zhang WH

    更新日期:2009-01-30 00:00:00

  • Comparing two measures of leaf photorespiration rate across a wide range of light intensities.

    abstract::Suppression of photorespiration by low O2 concentrations (Method 1) and simultaneous measurements of gas exchange and chlorophyll fluorescence (Method 2) are often used to estimate leaf photorespiration rate (Rp) of C3 plants. However, it is largely unknown whether Method 1 and Method 2 can be used equivalently in est...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Ye ZP,Liu YG,Kang HJ,Duan HL,Chen XM,Zhou SX

    更新日期:2019-09-01 00:00:00

  • The phenolic content and its involvement in the graft incompatibility process of various pear rootstocks (Pyrus communis L.).

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    pub_type: 杂志文章


    authors: Hudina M,Orazem P,Jakopic J,Stampar F

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Cakmak T,Cakmak ZE,Dumlupinar R,Tekinay T

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  • Recovery of uptake and assimilation of nitrate in Scenedesmus sp. previously exposed to elevated levels of Cu2+ and Zn2+.

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Tripathi BN,Mehta SK,Gaur JP

    更新日期:2004-05-01 00:00:00

  • Pectins esterification in the apoplast of aluminum-treated pea root nodules.

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    pub_type: 杂志文章


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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Zhang L,Yang M,Gao J,Jin S,Wu Z,Wu L,Zhang X

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  • Postharvest internal browning of pineapple fruit originates at the phloem.

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    pub_type: 杂志文章


    authors: Luengwilai K,Beckles DM,Siriphanich J

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    authors: Sievers A,Behrens HM,Buckhout TJ,Gradmann D

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  • The prohibitin genes in Arabidopsis thaliana: expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination.

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    journal_title:Journal of plant physiology

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    authors: De Diego JG,David Rodríguez F,Rodríguez Lorenzo JL,Cervantes E

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  • CO2 effects on the waterlogging response of 'Gisela 5' and 'Gisela 6' (Prunus cerasusxPrunus canescens) sweet cherry (Prunus avium) rootstocks.

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Pérez-Jiménez M,Hernández-Munuera M,Piñero MC,López-Ortega G,Del Amor FM

    更新日期:2017-06-01 00:00:00

  • Involvement of reactive oxygen species and auxin in serotonin-induced inhibition of primary root elongation.

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    journal_title:Journal of plant physiology

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    authors: Wan J,Zhang P,Sun L,Li S,Wang R,Zhou H,Wang W,Xu J

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Yue W,Nie X,Cui L,Zhi Y,Zhang T,Du X,Song W

    更新日期:2018-10-01 00:00:00

  • Hexokinase-mediated sugar signaling controls expression of the calcineurin B-like interacting protein kinase 15 gene and is perturbed by oxidative phosphorylation inhibition.

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Yim HK,Lim MN,Lee SE,Lim J,Lee Y,Hwang YS

    更新日期:2012-10-15 00:00:00

  • Functional analysis of a beta-1,3-glucanase gene (Tag1) with anther-specific RNA and protein accumulation using antisense RNA inhibition.

    abstract::A critical stage in pollen development is the dissolution of tetrads into free microspores. Tetrads are surrounded by a wall composed primarily of beta-1,3-glucan. At the completion of meiosis, tetrads are released into the anther locule after hydrolysis of the callose by a beta-1,3-glucanase complex. The cDNA corresp...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Bucciaglia PA,Zimmermann E,Smith AG

    更新日期:2003-11-01 00:00:00

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Plavcová L,Hronková M,Šimková M,Květoň J,Vráblová M,Kubásek J,Šantrůček J

    更新日期:2018-08-01 00:00:00

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Ruzvidzo O,Donaldson L,Valentine A,Gehring C

    更新日期:2011-09-15 00:00:00

  • MdMYB114 regulates anthocyanin biosynthesis and functions downstream of MdbZIP4-like in apple fruit.

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Jiang S,Sun Q,Zhang T,Liu W,Wang N,Chen X

    更新日期:2020-12-19 00:00:00

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    journal_title:Journal of plant physiology

    pub_type: 杂志文章


    authors: Wu P,Zhang S,Zhang L,Chen Y,Li M,Jiang H,Wu G

    更新日期:2013-10-15 00:00:00