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HPLC screening of natural vitamin E from mediterranean plant biofactories--a basic tool for pilot-scale bioreactors production of alpha-tocopherol.

Abstract：

:The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-speed milling with Waring blender for 40 s. alpha-Tocopherol was extracted from the nut tissue powder using dehydrated hexane fortified with 0.01% butylated hydroxytoluene (BHT, co-antioxidant). The rate of alpha-tocopherol accumulation showed differences among nut samples collected in different areas of Italy. Sarda Piccola nut biofactory contained higher amount (81.17 microg/g d.w) of alpha-tocopherol than other-local eleven Italian cultivar nuts. These results provide insight into the biofactory basis for alpha-tocopherol accumulation in hazelnut and give the suitable cultivar tissues to establish pilot-scale bioreactors production of natural bioactive vitamin E.

journal_name

J Plant Physiol

journal_title

Journal of plant physiology

authors

Sivakumar G,Bacchetta L,Gatti R,Zappa G

doi

10.1016/j.jplph.2005.04.018

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

1280-3

issue

11

eissn

0176-1617

issn

1618-1328

journal_volume

162

pub_type

杂志文章

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