Abstract:
:The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-speed milling with Waring blender for 40 s. alpha-Tocopherol was extracted from the nut tissue powder using dehydrated hexane fortified with 0.01% butylated hydroxytoluene (BHT, co-antioxidant). The rate of alpha-tocopherol accumulation showed differences among nut samples collected in different areas of Italy. Sarda Piccola nut biofactory contained higher amount (81.17 microg/g d.w) of alpha-tocopherol than other-local eleven Italian cultivar nuts. These results provide insight into the biofactory basis for alpha-tocopherol accumulation in hazelnut and give the suitable cultivar tissues to establish pilot-scale bioreactors production of natural bioactive vitamin E.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Sivakumar G,Bacchetta L,Gatti R,Zappa Gdoi
10.1016/j.jplph.2005.04.018subject
Has Abstractpub_date
2005-11-01 00:00:00pages
1280-3issue
11eissn
0176-1617issn
1618-1328journal_volume
162pub_type
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