Abstract:
:Over the last years, the discovery of various natural and the development of a row of engineered CRISPR/Cas nucleases have made almost every site of plant genomes accessible for the induction of specific changes. Newly developed tools open up a wide range of possibilities for the induction of genetic variability, from changing a single bp to Mbps, and thus to fine-tune plant performance. Whereas early approaches focused on targeted mutagenesis, recently developed tools enable the induction of precise and predefined genomic modifications. The use of base editors allows the substitution of single nucleotides, whereas the use of prime editors and gene targeting methods enables the induction of larger sequence modifications from a few bases to several kbp. Recently, through CRISPR/Cas-mediated chromosome engineering, it became possible to induce heritable inversions and translocations in the Mbp range. Thus, a novel way of breaking and fixing genetic linkages has come into reach for breeders. In addition, sequence-specific recruitment of various factors involved in transcriptional and post-transcriptional regulation has been shown to provide an additional class of methods for the fine tuning of plant performance. In this review, we provide an overview of the most recent progress in the field of CRISPR/Cas-based tool development for plant genome engineering and try to evaluate the importance of these developments for breeding and biotechnological applications.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Capdeville N,Merker L,Schindele P,Puchta Hdoi
10.1016/j.jplph.2020.153332subject
Has Abstractpub_date
2020-12-15 00:00:00pages
153332eissn
0176-1617issn
1618-1328pii
S0176-1617(20)30222-4journal_volume
257pub_type
杂志文章,评审abstract::Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a "dry-down" experiment under c...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.12.014
更新日期:2014-04-15 00:00:00
abstract::The uptake of liposomal contents into Catharanthus roseus protoplasts has been quantitated. Hi.phest uptake was obtained from positively charged vesicles (1.09% of vesicle contents per 10(7) protoplasts) in the presence of 10% w/v polyethylene glycol 4000. Significant uptake was also observed from negatively charged v...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80014-0
更新日期:1984-11-01 00:00:00
abstract::Triacylglycerols (TAGs) are the most important energy storage form in oilseed crops. Diacylglycerol acyltransferase (DGAT) catalyzes the rate-limiting step of the Kennedy pathway of TAG biosynthesis. To date, little is known about the regulation of DGAT activity in peanut (Arachis hypogaea), an agronomically important...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.009
更新日期:2017-12-01 00:00:00
abstract::PAM fluorometry showed that the orchid Vanda sp (Gaud ex Pfitzers, Vandeae) had photosynthetic electron transport yields in leaves reaching ≈ 0.617 ± 0.262 at midday. Yield decayed exponentially as irradiance increased (Y½ = 128 ± 12.4 μmol photon m-2 s-1). Optimum irradiance (Eopt) for ETR (Photosynthetic Electron Tr...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153187
更新日期:2020-08-01 00:00:00
abstract::Flowering time control is important for fruit production in Fragaria × ananassa. The flowering inhibition pathway has been extensively elucidated in the woodland strawberry, Fragaria vesca, whereas the factors involved in its promotion remain unclear. In this study, we investigated the environmental responses of F. × ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.01.007
更新日期:2015-04-01 00:00:00
abstract::Reflectance spectra of leaves of winter wheat, maize, sugar beet and wild vine have been measured. Chlorophyll variations induce changes in the first derivatives of the reflectance spectra. A correlation of various derivative indices such as D(725)/D(702), D(lw)/D(sw), D(lw)/D(702), D(725)/D(sw,)D(715)/D(702), D(m)/D(...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.11.007
更新日期:2007-12-01 00:00:00
abstract::Ethylene responsive transcription factors (ERFs) can be grouped into different classes with either gene activator or repressor activity. We have isolated a tomato ERF cDNA clone (LeERF3b) with sequence similarity to class II (repressor class) of the ERF family, which is regulated differently from Pti4 (a tomato ERF do...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.03.006
更新日期:2008-04-18 00:00:00
abstract::Environmental temperature change may induce modifications in membrane lipid properties and composition, which account for different physiological responses among plant species. Coffee plants, as many tropical species, are particularly sensitive to cold, but genotypes can present differences that can be exploited to im...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.07.007
更新日期:2014-02-15 00:00:00
abstract::Nodule conductance to O2 diffusion has been involved as a major factor of the inhibition of N2 fixation by soil salinity that severely reduces the production of grain legumes. In order to determine the effect of this constraint on the nodule conductance, oxygen uptake by the nodulated roots of Cicer arietinum was meas...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.05.016
更新日期:2007-08-01 00:00:00
abstract::The microinjection of fluorescent probes into live cells is an essential component in the toolbox of modern cell biology. Microinjection techniques include the penetration of the plasma membrane and, if present, the cell wall with micropipettes, and the application of pressure or electrical currents to drive the micro...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153060
更新日期:2020-01-01 00:00:00
abstract::Most of the studies carried out on Fe deficiency condition in arboreous plants have been performed, with the exception of those carried out on plants grown in the field, in hydroponic culture utilizing a total iron depletion growth condition. This can cause great stress to plants. By introducing Fe deficiency induced ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.01.007
更新日期:2009-07-15 00:00:00
abstract::Previous studies have shown that plants developed under high relative air humidity (RH>85%) develop malfunctioning stomata and therefor have increased transpiration and reduced desiccation tolerance when transferred to lower RH conditions and darkness. In this study, plants developed at high RH were exposed to daily V...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.12.011
更新日期:2017-04-01 00:00:00
abstract::Stomata formed at high relative air humidity (RH) close less as leaf dries; an effect that varies depending on the genotype. We here quantified the contribution of each stomatal response characteristic to the higher water loss of high RH-grown plants, and assessed the relationship between response characteristics and ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.04.007
更新日期:2013-10-15 00:00:00
abstract::Changes in phenolics (PhC - phenolic compounds) measured as UV-absorbing compounds (UVAC) and their localization as well as growth, lipid peroxidation (TBARS level) and proline (Pro) level in three-day-old roots of seedlings (T(o) stage) obtained from hydroprimed (H) and hydroprimed with melatonin (H-MEL) seeds after ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.08.011
更新日期:2012-01-01 00:00:00
abstract::In tightly closed environments used for human life support in space, carbon dioxide (CO2) partial pressures can reach 500 to 1000 Pa, which may be supraoptimal or toxic to plants used for life support. To study this, soybeans [Glycine max (L.) Merr. cvs. McCall and Pixie] were grown for 90 days at 50, 100, 200, and 5...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/s0176-1617(11)80959-4
更新日期:1993-01-01 00:00:00
abstract::After several years of close contacts and extensive discussion between various plant physiologists of different European countries, the Federation of European Societies of Plant Physiology (FESPP) was established in 1978 in Edinburgh. The aim of the FESPP was and remains to promote up-to-date plant physiology research...
journal_title:Journal of plant physiology
pub_type: 历史文章,杂志文章
doi:10.1078/0176-1617-01371
更新日期:2004-06-01 00:00:00
abstract::Plants counteract Cd toxicity by activating cellular stress responses. The simultaneous exogenous application of methyl jasmonate (MeJA) and methyl-β-cyclodextrin (CD) before Cd exposure improved the response of Arachis hypogaea hairy root culture to the unfavourable effects of Cd toxicity. At 24 h after elicitation, ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153278
更新日期:2020-11-01 00:00:00
abstract::Exogenously applied H2O2 (50, 100 and 200mM) to Mesembryanthemum crystallinum root medium induced a transition from C3 to Crassulacean Acid Metabolism (CAM), as evaluated by diurnal malate (Δmal) fluctuations. A very high concentration of H2O2 (400mM) reduced Δmal below the value measured in control plants. An increas...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.05.021
更新日期:2016-08-01 00:00:00
abstract::A vacuolar proton pyrophosphatase cDNA clone was isolated from Sorghum bicolor (SbVPPase) using end-to-end gene-specific primer amplification. It showed 80-90% homology at the nucleotide and 85-95% homology at the amino acid level with other VPPases. The gene was introduced into expression vector pCAMBIA1301 under the...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2014.02.001
更新日期:2014-06-15 00:00:00
abstract::We present here the comparative protective potentiality of exogenously applied polyamines (PAs), namely spermidine (Spd) and spermine (Spm), in mitigating NaCl toxicity and inducing short-term salinity tolerance in three indica rice varieties, namely M-1-48 (salt-sensitive), Nonabokra (salt-tolerant) and Gobindobhog (...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.07.009
更新日期:2011-03-01 00:00:00
abstract::The flavor and taste of fruits are often determined by terpenes. We identified three cDNAs encoding putative terpene synthases from olive fruits of cv. Frantoio and Grignano. Heterologous expression in a bacterial system demonstrated that one of the terpene synthases, OeGES1, was an active monoterpene synthase that co...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.01.021
更新日期:2012-06-15 00:00:00
abstract::The differences between two maize (Zea mays L.) inbred lines and their F1 hybrids in their response to chilling periods of various duration (1, 2, 3 or 4 weeks) and subsequent return to optimum temperatures were analysed by the measurement of the photosystem (PS) 1 and 2 activity, the photosynthetic pigments' content ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.04.016
更新日期:2007-07-01 00:00:00
abstract::Using a direct somatic embryogenesis system in carrot, we examined the role of DNA methylation in the change of cellular differentiation state, from somatic to embryogenic. 5-Azacytidine (aza-C), an inhibitor of DNA methylation suppressed the formation of embryogenic cell clumps from epidermal carrot cells. Aza-C also...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.05.013
更新日期:2005-01-01 00:00:00
abstract::Common sage (Salvia officinalis L., Lamiaceae) is one of the most important medicinal and aromatic plants, with antioxidant, antimicrobial, spasmolytic, astringent, antihidrotic and specific sensorial properties. The essential oil of the plant, composed mainly of the monoterpenes 1,8-cineole, alpha-thujone, beta-thujo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.009
更新日期:2010-07-01 00:00:00
abstract::Plants regulate a number of primary metabolites, including carbohydrates, organic acids, and amino acids, in response to UV-B radiation. Therefore, it is essential to understand the time-dependent response of rice plants to UV-B stress. This study focused on the response of plants to UV-B at different leaf development...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.02.007
更新日期:2018-09-01 00:00:00
abstract::An essential step in the biosynthesis of bioactive brassinosteroids (BRs) in plants is the hydroxylation at C-22, a reaction catalyzed by P450 enzymes of the CYP90B and CYP724B subfamilies. Genes for both types of enzymes are present in many species, and in rice (Oryza sativa) and tomato (Solanum lycopersicum) both CY...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.10.013
更新日期:2012-03-01 00:00:00
abstract::The plant vacuole constitutes a large transient storage compartment for nutrients, proteins and metabolites, and is a major cellular sink for toxic waste compounds. Amino acids can cross the vacuolar membrane via specific transport proteins, which are molecularly not well characterized. Two members of a small subfamil...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.11.012
更新日期:2014-05-01 00:00:00
abstract::Seeds of Arabidopsis thaliana were sent to space and germinated in orbit. Seedlings grew for 4d and were then fixed in-flight with paraformaldehyde. The experiment was replicated on the ground in a Random Positioning Machine, an effective simulator of microgravity. In addition, samples from a different space experimen...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.08.012
更新日期:2010-02-15 00:00:00
abstract::Chlorophyll-oxidase in barley thylakoids was activated by linolenic acid. The reaction products from endogenous chlorophylls were investigated by thin layer and high performance liquid chromatography. Within 2-10 min a new pigment was formed which migrated identically with authentic 13(2)-hydroxychlorophyll a (1a). Af...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80054-1
更新日期:1984-06-01 00:00:00
abstract::Alterations in temperature adaptation processes and changes in the content of stress-related compounds, polyamines and salicylic acid were evaluated in Atnoa1 (NO-associated 1) Arabidopsis mutant. The F(v)/F(m) chlorophyll-a fluorescence induction parameter and the actual quantum yield were significantly lower in the ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.01.021
更新日期:2011-07-15 00:00:00