Abstract：
:Over the last years, the discovery of various natural and the development of a row of engineered CRISPR/Cas nucleases have made almost every site of plant genomes accessible for the induction of specific changes. Newly developed tools open up a wide range of possibilities for the induction of genetic variability, from changing a single bp to Mbps, and thus to fine-tune plant performance. Whereas early approaches focused on targeted mutagenesis, recently developed tools enable the induction of precise and predefined genomic modifications. The use of base editors allows the substitution of single nucleotides, whereas the use of prime editors and gene targeting methods enables the induction of larger sequence modifications from a few bases to several kbp. Recently, through CRISPR/Cas-mediated chromosome engineering, it became possible to induce heritable inversions and translocations in the Mbp range. Thus, a novel way of breaking and fixing genetic linkages has come into reach for breeders. In addition, sequence-specific recruitment of various factors involved in transcriptional and post-transcriptional regulation has been shown to provide an additional class of methods for the fine tuning of plant performance. In this review, we provide an overview of the most recent progress in the field of CRISPR/Cas-based tool development for plant genome engineering and try to evaluate the importance of these developments for breeding and biotechnological applications.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Capdeville N,Merker L,Schindele P,Puchta Hdoi
10.1016/j.jplph.2020.153332subject
Has Abstractpub_date
2020-12-15 00:00:00pages
153332eissn
0176-1617issn
1618-1328pii
S0176-1617(20)30222-4journal_volume
257pub_type
杂志文章,评审abstract：:Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a "dry-down" experiment under c...
journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.01.007
更新日期：2015-04-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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abstract：:Ethylene responsive transcription factors (ERFs) can be grouped into different classes with either gene activator or repressor activity. We have isolated a tomato ERF cDNA clone (LeERF3b) with sequence similarity to class II (repressor class) of the ERF family, which is regulated differently from Pti4 (a tomato ERF do...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.03.006
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.07.007
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.01.007
更新日期：2009-07-15 00:00:00
abstract：:Previous studies have shown that plants developed under high relative air humidity (RH>85%) develop malfunctioning stomata and therefor have increased transpiration and reduced desiccation tolerance when transferred to lower RH conditions and darkness. In this study, plants developed at high RH were exposed to daily V...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.12.011
更新日期：2017-04-01 00:00:00
abstract：:Stomata formed at high relative air humidity (RH) close less as leaf dries; an effect that varies depending on the genotype. We here quantified the contribution of each stomatal response characteristic to the higher water loss of high RH-grown plants, and assessed the relationship between response characteristics and ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.04.007
更新日期：2013-10-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.08.011
更新日期：2012-01-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/s0176-1617(11)80959-4
更新日期：1993-01-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 历史文章,杂志文章
doi：10.1078/0176-1617-01371
更新日期：2004-06-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2020.153278
更新日期：2020-11-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.05.021
更新日期：2016-08-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.04.016
更新日期：2007-07-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.05.013
更新日期：2005-01-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.12.009
更新日期：2010-07-01 00:00:00
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journal_title：Journal of plant physiology
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更新日期：2018-09-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2012-03-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2014-05-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.08.012
更新日期：2010-02-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/S0176-1617(84)80054-1
更新日期：1984-06-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.01.021
更新日期：2011-07-15 00:00:00
