Abstract：
:The plant vacuole constitutes a large transient storage compartment for nutrients, proteins and metabolites, and is a major cellular sink for toxic waste compounds. Amino acids can cross the vacuolar membrane via specific transport proteins, which are molecularly not well characterized. Two members of a small subfamily of the cationic amino acid transporters, AtCAT2 and AtCAT4, were primarily localized at the tonoplast when tagged with GFP. The closely related AtCAT3, by contrast, was detected in the endoplasmic reticulum membrane. The exchange of a di-acidic motif at the carboxy-tail affected their sub-cellular localization, with larger effects visible in transiently transformed protoplasts compared to stably expressing plant lines. The genes have broad, partially overlapping tissue expression, with CAT2 dominating in most tissues. Loss-of-function mutants of individual CATs showed no visible phenotype under various conditions, but the overall tissue concentration of amino acids was increased in soil-grown cat2 mutants. The data suggest that CAT2 is a critical target of leaf amino acid concentrations and manipulation of this tonoplast transporter can significantly alter total tissue amino acid concentrations.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Yang H,Krebs M,Stierhof YD,Ludewig Udoi
10.1016/j.jplph.2013.11.012subject
Has Abstractpub_date
2014-05-01 00:00:00pages
594-601issue
8eissn
0176-1617issn
1618-1328pii
S0176-1617(13)00461-6journal_volume
171pub_type
杂志文章abstract：:CBFs (C-repeat binding factors) are transcription factors that are rapidly induced by low temperature and that recognize the CRT/DRE element in the promoter of a set of cold regulated genes, the CBF regulon. Dehydrins are proteins that accumulate in plants under stress conditions, such as low temperature, and some for...
journal_title：Journal of plant physiology
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01045
更新日期：2003-06-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.08.012
更新日期：2010-02-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2010-04-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.02.003
更新日期：2005-12-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2009-07-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.03.012
更新日期：2007-05-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.02.011
更新日期：2009-01-30 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01022
更新日期：2004-03-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.01.021
更新日期：2012-06-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.07.008
更新日期：2011-12-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2003-08-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.02.008
更新日期：2017-06-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01038
更新日期：2004-01-01 00:00:00
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：1984-09-01 00:00:00
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journal_title：Journal of plant physiology
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更新日期：1985-04-01 00:00:00
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of plant physiology
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更新日期：2003-04-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2016-03-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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journal_title：Journal of plant physiology
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更新日期：2008-07-07 00:00:00
