Abstract:
:Plant lipid transfer proteins (LTPs) are low-molecular-mass proteins whose biological function still remains elusive. They are synthesized with a signal peptide that drives them to the secretory pathway. We have previously described the occurrence of an apoplastic LTP named Ha-AP10, present in sunflower seeds. Using a biochemical approach we now demonstrate that a fraction of Ha-AP10 is perispherically bound to membranes of germinating seeds. Purification of plasma membranes revealed the presence of Ha-AP10 in this fraction. Fluorimmunolocalization studies on germinating sunflower seeds demonstrated that in addition to the apoplastic and plasma membrane localization, Ha-AP10 is also present intracellularlly associated to unidentified structures. This varied distribution of Ha-AP10 in sunflower seeds may give novel clues to understand the role of LTPs in seed physiology.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Pagnussat LA,Lombardo C,Regente M,Pinedo M,Martín M,de la Canal Ldoi
10.1016/j.jplph.2008.11.005subject
Has Abstractpub_date
2009-05-15 00:00:00pages
797-806issue
8eissn
0176-1617issn
1618-1328pii
S0176-1617(08)00340-4journal_volume
166pub_type
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