Abstract：
:Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To identify these proteins in more detail, the bands with RNase activity were excised from gel and subjected to trypsin digestion followed by LC-MS/MS analysis. This analysis revealed that S-RNases accumulate in nectar from Petunia hybrida, where they should carry out a biological function different from self-pollen rejection. In addition, other proteins were identified by the LC-MS/MS analysis. These proteins include a peroxidase, an endochitinase, and a putative fructokinase. Each of these proteins contained a secretory signal sequence that marked them as potential nectar proteins. We developed RT-PCR assays for each of these five proteins and demonstrated that each of these proteins was expressed in the petunia floral nectary. A discussion of the role of these proteins in antimicrobial activity in nectar is presented.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Hillwig MS,Kanobe C,Thornburg RW,Macintosh GCdoi
10.1016/j.jplph.2010.10.002subject
Has Abstractpub_date
2011-05-01 00:00:00pages
734-8issue
7eissn
0176-1617issn
1618-1328pii
S0176-1617(10)00479-7journal_volume
168pub_type
杂志文章abstract：:A sand culture experiment was conducted to answer the question whether or not exogenous KNO(3) can alleviate adverse effects of salt stress in winter wheat by monitoring plant growth, K(+)/Na(+) accumulation and the activity of some antioxidant enzymes. Seeds of two wheat cultivars (CVs), DK961 (salt-tolerant) and JN1...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.01.001
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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abstract：:In prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. Here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
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abstract：:Fruit ripening is a complex process involving many physiological and biochemical changes, including those for ethylene, carotenoid, and cell wall metabolism. Tomato (Solanum lycopersicum) serves as a research model for fruit development and ripening because it possesses numerous favorable genetic features. In this stu...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.01.014
更新日期：2016-04-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153107
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.04.007
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journal_title：Journal of plant physiology
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.04.005
更新日期：2018-07-01 00:00:00
abstract：:EDDHA added in an optimal concentration (20.5 mumol.L-1) to a modified Pirson-Seidel nutrient solution induces flowering in some clones of the species Lemna minor, Lemna gibba and Spirodela polyrrhiza, which in the absence of EDDHA in the same nutrient solution do not flower. By adding EDDHA (20.5 mumol.L-1), floral i...
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pub_type： 杂志文章
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.10.014
更新日期：2018-11-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.07.004
更新日期：2018-10-01 00:00:00
abstract：:Plants regulate a number of primary metabolites, including carbohydrates, organic acids, and amino acids, in response to UV-B radiation. Therefore, it is essential to understand the time-dependent response of rice plants to UV-B stress. This study focused on the response of plants to UV-B at different leaf development...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.02.007
更新日期：2018-09-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.11.013
更新日期：2007-12-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.03.016
更新日期：2012-08-15 00:00:00
abstract：:A single polypeptide catalysing the two step conversion of orotate to UMP in cultured tomato cells was purified to near homogeneity as judged by analytical disc gel electrophoresis. After electrophoresis of the dodecyl sulphate denaturated enzyme one single protein band appeared from which both enzyme activities could...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/S0176-1617(84)80109-1
更新日期：1984-01-01 00:00:00
abstract：:The effect of excess copper on the expression of soluble proteins in 10-day old Phaseolus vulgaris seedlings was studied with two-dimensional electrophoresis and mass spectrometry, to find sensitive biochemical markers of exposure. Despite major differences in root Cu contents, both 15 and 50 microM Cu treatments resu...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.07.018
更新日期：2005-04-01 00:00:00
abstract：:In order to examine whether polyamines (PAs) modify the functioning of the scavenging system and oxidative stress levels in water-stressed plants, cucumber (Cucumis sativus L.) seedlings were treated with spermidine (Spd) prior to dehydration, and stress-evoked changes in superoxide dismutase (SOD) (EC 1.15.1.1), cata...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.02.005
更新日期：2008-03-13 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.07.011
更新日期：2016-09-01 00:00:00
abstract：:High salinity is a major abiotic stress that affects the growth and development of plants. This type of stress can influence flowering, the production of crops, defense mechanisms and other physiological processes. Previous studies have attempted to elucidate salt-tolerance mechanisms to improve plant growth and produ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-00525
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journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2011-03-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.04.005
更新日期：2006-11-01 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.04.005
更新日期：2012-08-15 00:00:00
abstract：:Fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (Cucumis melo L.). In this work the presence of a class III chitinase was examined by immunological techniques in the root and stem base of a susceptible (cv. Galia) and a resistant (cv. Bredor) melon during the infe...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.02.004
更新日期：2006-01-01 00:00:00
abstract：:Durian (Durio zibethinus) fruit was harvested at the commercially mature stage and stored at 25°C. Durian fruit have 3-5 longitudinal dehiscence zones (DZs) in the peel, which are up to 40cm long and 2cm thick in large fruit. Dehiscence started a week after harvest, was hastened by exogenous ethylene, and delayed by 1...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.04.005
更新日期：2015-06-15 00:00:00
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-00194
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journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.06.013
更新日期：2015-07-20 00:00:00
abstract：:Seedlings of Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and Alemow (Citrus macrophylla Wester) were inoculated with a mixture of AM fungi (Rhizophagus irregularis and Funneliformis mosseae) (+AM), or left non-inoculated (-AM). From forty-five days after fungal inoculation onwards, half of +AM or -AM plants were ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.06.006
更新日期：2014-01-01 00:00:00
abstract：:The ultrastructure of mesophyll cells was studied in leaves of the Triticum aestivum L. cv. "Trizo" seedlings after two weeks of growth on soil contaminated by Pb and/or Se. The soil treatments: control; (Pb1) 50mgkg-1; (Pb2) 100mgkg-1; (Se1) 0.4mgkg-1; (Se2) 0.8mgkg-1; (Pb1+Se1); (Pb1+Se2); (P2+Se1); and (Pb2+Se2) we...
journal_title：Journal of plant physiology
pub_type： 杂志文章
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更新日期：2017-12-01 00:00:00
abstract：:Our previous study suggests that salicylic acid mediates tolerance in barley plants to paraquat (Ananieva et al. 2002). To further define the role of SA in paraquat induced responses, we analysed the capacity of the antioxidative defence system by measuring the activities of several antioxidative enzymes: superoxide d...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01022
更新日期：2004-03-01 00:00:00
