Abstract：
:A single polypeptide catalysing the two step conversion of orotate to UMP in cultured tomato cells was purified to near homogeneity as judged by analytical disc gel electrophoresis. After electrophoresis of the dodecyl sulphate denaturated enzyme one single protein band appeared from which both enzyme activities could be renaturated by addition of Triton X-100. As introduced by McClard et al. (Biochemistry 19, 4699-4706, 1980) for the mammalian system, this plant enzyme should be termed UMP synthase, consequently. The enzyme consists of a single polypeptide chain of a molecular weight of about 51,000. Molecular weight determination by gel filtration under non-denaturating conditions gave a value of about 100,000, suggesting dimer formation in vivo. The enzyme contains thiol groups essential for enzyme activity. Kinetic characteristics of the orotate phosphoribosyltransferase activity are: pH optimum 8.0, Km values for orotate and PRPP of 4.5 and 5.4µM, respectively. Orotidine-5'-phosphate decarboxylase activity is optimal between pH 7.2 and 8.5, the Km value for orotidine-5'-phosphate 2 µM.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Walther R,Wald K,Glund K,Tewes Adoi
10.1016/S0176-1617(84)80109-1subject
Has Abstractpub_date
1984-01-01 00:00:00pages
301-11issue
4eissn
0176-1617issn
1618-1328pii
S0176-1617(84)80109-1journal_volume
116pub_type
杂志文章abstract：:Plastidial degradation of transitory starch yields mainly maltose and glucose. Following the export into the cytosol, maltose acts as donor for a glucosyl transfer to cytosolic heteroglycans as mediated by a cytosolic transglucosidase (DPE2; EC 2.4.1.25) and the second glucosyl residue is liberated as glucose. The cyt...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.09.008
更新日期：2011-08-15 00:00:00
abstract：:Common bean, one of the most important legumes for human consumption, may have drastic reduction in yield due to anthracnose, a disease caused by the fungus Colletotrichum lindemuthianum. Rapid induction of the plant defense mechanisms is essential to establish an incompatible interaction with this pathogenic fungus. ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.04.003
更新日期：2012-08-15 00:00:00
abstract：:Low temperature is one of the primary causes of economic loss in agricultural production, and in this regard, expansin proteins are known to play important roles in plant growth and responses to various abiotic stresses and plant hormones. In order to elucidate the roles of expansin genes in the response of Dongnongdo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153004
更新日期：2019-09-01 00:00:00
abstract：:Hyacinth (Hyacinthus orientalis) bulbs infected by Fusarium oxysporum showed the symptoms of gummosis. The purpose of this study was to clarify the hormonal regulation of gummosis and composition of gums from hyacinth bulbs. The application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2014.10.007
更新日期：2015-02-01 00:00:00
abstract：:Replacement of N(2) by argon in the air around nodules directs nitrogenase electron flow in its total onto H(+) resulting in increased nodule H(2) evolution (total nitrogenase activity (TNA)). However, argon application induces a so-called argon-induced decline in nitrogenase activity (Ar-ID) connected with decreased ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.03.014
更新日期：2010-09-01 00:00:00
abstract：:Silicon (Si) may be involved in metabolic, physiological, and/or structural activity in higher plants exposed to abiotic and biotic stresses. This has not yet been determined due to the absence of direct evidence that it is part of the molecule of an essential plant constituent or metabolite. The aim of this study was...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.04.011
更新日期：2010-10-15 00:00:00
abstract：:The toxicity and effects on protein synthesis of the phthalate esters diethyl phthalate (DEP) and di(2-ethylhexyl) phthalate (DEHP) was studied in radish seedings (Raphanus sativus cv. Kööpenhaminan tori). Phthalate esters are a class of commercially important compounds used mainly as plasticizers in high molecular-we...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-00525
更新日期：2003-09-01 00:00:00
abstract：:Gibberellins (GAs) are the plant hormones that control many aspects of plant growth and development, including stem elongation. Genes encoding enzymes related to the GA biosynthetic and metabolic pathway have been isolated and characterized in many plant species. Gibberellin 2-oxidase (GA2ox) catalyzes bioactive GAs o...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.05.002
更新日期：2013-11-01 00:00:00
abstract：:Most of the studies carried out on Fe deficiency condition in arboreous plants have been performed, with the exception of those carried out on plants grown in the field, in hydroponic culture utilizing a total iron depletion growth condition. This can cause great stress to plants. By introducing Fe deficiency induced ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.01.007
更新日期：2009-07-15 00:00:00
abstract：:Germination is a complex process, highly dependent on various environmental factors, including temperature and water availability. Germinating soybean seeds are especially vulnerable to unfavorable environmental conditions and exposure to long-term abiotic stresses may result in diminishing much of the yield and most ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.11.020
更新日期：2013-03-15 00:00:00
abstract：:Microbial consortia may provide protection against pathogenic ingress via enhancing plant defense responses. Pseudomonas aeruginosa PJHU15, Trichoderma harzianum TNHU27 and Bacillus subtilis BHHU100 were used either singly or in consortia in the pea rhizosphere to observe proteome level changes upon Sclerotinia sclero...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.05.004
更新日期：2015-06-15 00:00:00
abstract：:Hydroxyproline-rich glycoproteins (HRGPs) are plant cell wall proteins related to plant growth and development, and extensins (EXTs) are a subfamily of HRGPs. In this study, the function of HRGPs, especially EXTs, was investigated in the pollen tube and style cell growth of Nicotiana tabacum L. By using the techniques...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2014.02.010
更新日期：2014-07-15 00:00:00
abstract：:Leaf discs of bean, maize, and wheat were incubated in the presence of either 2-(14)C mevalonic acid or methyl-(14)C choline chloride and varying concentrations of abscisic acid. Abscisic acid inhibited, dependent on concentration, the incorporation of mevalonate into free sterols and stimulated the incorporation of c...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/S0176-1617(85)80197-8
更新日期：1985-03-01 00:00:00
abstract：:The endophytic Bacillus amyloliquefaciens YTB1407 was previously reported to promote the growth of sweet potato (Ipomoea batatas cv. Yanshu 25). Here, we demonstrate in both in vitro and pot trial assays that pre-treatment with YTB1407 suspension could enhance resistance against root rot disease and black rot disease,...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2020.153260
更新日期：2020-10-01 00:00:00
abstract：:The leaf is considered the most important vegetative organ of tank epiphytic bromeliads due to its ability to absorb and assimilate nutrients. However, little is known about the physiological characteristics of nutrient uptake and assimilation. In order to better understand the mechanisms utilized by some tank epiphyt...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.01.008
更新日期：2011-07-15 00:00:00
abstract：:A sand culture experiment was conducted to answer the question whether or not exogenous KNO(3) can alleviate adverse effects of salt stress in winter wheat by monitoring plant growth, K(+)/Na(+) accumulation and the activity of some antioxidant enzymes. Seeds of two wheat cultivars (CVs), DK961 (salt-tolerant) and JN1...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.01.001
更新日期：2008-09-29 00:00:00
abstract：:Hormones are included in the essential elements that control the induction of flowering. Ethylene is thought to be a strong inhibitor of flowering in short day plants (SDPs), whereas the involvement of abscisic acid (ABA) in the regulation of flowering of plants is not well understood. The dual role of ABA in the phot...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.04.009
更新日期：2008-12-01 00:00:00
abstract：:The expression of two genes, namely Mir1 and Mir3 and the abundance of their encoded proteins, the putative miraculin-like proteins, MLP1 and MLP3, showing similarity to the Kunitz family of protease inhibitors, were monitored in the leaves of the citrus variety, 'Clementine' after Tetranychus urticae infestation and ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.08.001
更新日期：2014-01-01 00:00:00
abstract：:Reports on the effect of nitric oxide (NO) or reactive oxygen species (ROS) on photosynthesis and respiration in leaf tissues are intriguing; therefore, the effects of exogenous addition of sodium nitroprusside (SNP, releases NO) or H2O2 on the photosynthetic O2 evolution and respiratory O2 uptake by mesophyll protopl...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2020.153133
更新日期：2020-01-01 00:00:00
abstract：:Since sweetness is one of the most important qualities of many fruits, and since sugars are translocated from leaves to fruits, the present study investigates photosynthetic activity, activity of sugar metabolizing enzymes, sugar content in leaves and fruits and endogenous levels of hydrogen peroxide in leaves of melo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.08.007
更新日期：2009-04-01 00:00:00
abstract：:Blueberry (Vaccinium sp.) is thought to display a preference for the ammonium (NH4+) form over the nitrate (NO3-) form of inorganic nitrogen (N). This N-source preference has been associated with a generally low capacity to assimilate the NO3- form of N, especially within the shoot tissues. Nitrate assimilation is med...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.05.014
更新日期：2017-09-01 00:00:00
abstract：:Syringyl lignins result from the oxidative polymerization of sinapyl alcohol in a reaction mediated by syringyl (basic) peroxidases. Several peroxidases have been identified in the genome of Arabidopsis thaliana as close homologues to ZePrx, the best characterized basic peroxidase so far, but none of these has been di...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2014.11.006
更新日期：2015-03-01 00:00:00
abstract：:Large molecules require a nuclear localization signal (NLS) for translocation into the nucleus. Classical NLSs are rich in basic amino acids and they represent three groups, based on their structural features: SV40 T-antigen-type, yeast mating factor Matalpha-2-type, and bipartite NLSs. DNA-binding-with-one-finger (DO...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.11.016
更新日期：2010-05-01 00:00:00
abstract：:Fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (Cucumis melo L.). In this work the presence of a class III chitinase was examined by immunological techniques in the root and stem base of a susceptible (cv. Galia) and a resistant (cv. Bredor) melon during the infe...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.02.004
更新日期：2006-01-01 00:00:00
abstract：:Some intracellular organelles found in eukaryotes such as plants have arisen through the endocytotic engulfment of prokaryotic cells. This accounts for the presence of plant membrane intrinsic proteins that have homologs in prokaryotic cells. Other organelles, such as those of the endomembrane system, are thought to h...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.09.022
更新日期：2014-05-15 00:00:00
abstract：:pea-MADS4 (PEAM4) regulates floral morphology in Pisum sativum L., however, its molecular mechanisms still remain unclear. Virus-induced gene silencing (VIGS) is a recently developed reverse genetic approach that facilities an easier and more rapid study of gene functions. In this study, the PEAM4 gene was effectively...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.08.010
更新日期：2014-01-15 00:00:00
abstract：:A vacuolar proton pyrophosphatase cDNA clone was isolated from Sorghum bicolor (SbVPPase) using end-to-end gene-specific primer amplification. It showed 80-90% homology at the nucleotide and 85-95% homology at the amino acid level with other VPPases. The gene was introduced into expression vector pCAMBIA1301 under the...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2014.02.001
更新日期：2014-06-15 00:00:00
abstract：:The microinjection of fluorescent probes into live cells is an essential component in the toolbox of modern cell biology. Microinjection techniques include the penetration of the plasma membrane and, if present, the cell wall with micropipettes, and the application of pressure or electrical currents to drive the micro...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153060
更新日期：2020-01-01 00:00:00
abstract：:The epiphytic fern Pleopeltis polypodioides can tolerate repeated drying and rehydration events without conspicuous damage. To understand the biochemical principles of drought-tolerance, we analyzed the effect of dehydration and rehydration at 25 °C on hydroperoxide and lipid hydroperoxide, the activities of antioxida...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.05.006
更新日期：2018-09-01 00:00:00
abstract：:Durian (Durio zibethinus) fruit was harvested at the commercially mature stage and stored at 25°C. Durian fruit have 3-5 longitudinal dehiscence zones (DZs) in the peel, which are up to 40cm long and 2cm thick in large fruit. Dehiscence started a week after harvest, was hastened by exogenous ethylene, and delayed by 1...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.04.005
更新日期：2015-06-15 00:00:00
