Abstract:
:A vacuolar proton pyrophosphatase cDNA clone was isolated from Sorghum bicolor (SbVPPase) using end-to-end gene-specific primer amplification. It showed 80-90% homology at the nucleotide and 85-95% homology at the amino acid level with other VPPases. The gene was introduced into expression vector pCAMBIA1301 under the control of the cauliflower mosaic virus 35S (CaMV35S) promoter and transformed into Agrobacterium tumifaciens strain LBA4404 to infect embryogenic calli of finger millet (Eleusine coracana). Successful transfer of SbVPPase was confirmed by a GUS histochemical assay and PCR analysis. Both, controls and transgenic plants were subjected to 100 and 200mM NaCl and certain biochemical and physiological parameters were studied. Relative water content (RWC), plant height, leaf expansion, finger length and width and grain weight were severely reduced (50-70%), and the flowering period was delayed by 20% in control plants compared to transgenic plants under salinity stress. With increasing salt stress, the proline and chlorophyll contents as well as the enzyme activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and glutathione reductase (GR) increased by 25-100% in transgenics, while malondialdehyde (MDA) showed a 2-4-fold decrease. The increased activities of antioxidant enzymes and the reduction in the MDA content suggest efficient scavenging of reactive oxygen species (ROS) in transgenics and, as a consequence, probably alleviation of salt stress. Also, the leaf tissues of the transgenics accumulated 1.5-2.5-fold higher Na(+) and 0.4-0.8-fold higher K(+) levels. Together, these results clearly demonstrate that overexpression of SbVPPase in transgenic finger millet enhances the plant's performance under salt stress.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Anjaneyulu E,Reddy PS,Sunita MS,Kishor PB,Meriga Bdoi
10.1016/j.jplph.2014.02.001subject
Has Abstractpub_date
2014-06-15 00:00:00pages
789-98issue
10eissn
0176-1617issn
1618-1328pii
S0176-1617(14)00035-2journal_volume
171pub_type
杂志文章abstract::A ribosome undergoes significant conformational changes during elongation of a polypeptide chain, and these are correlated with structural changes of rRNAs. We tested 15 different oligonucleotides complementary to the selected, highly conserved seqences of rRNAs (L-rRNA, 5S rRNA and tRNA) important in protein biosynth...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.03.016
更新日期:2006-03-01 00:00:00
abstract::Sun (Chrysanthemum morifolium) and shade (Spathiphyllum wallisii) plants were used to study the effects of drought, heat and high illumination. The stress conditions caused a greater accumulation of hydrogen peroxide in Chrysanthemum morifolium than in Spathiphyllum wallisii leaves. They also resulted in down-regulati...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.013
更新日期:2010-06-15 00:00:00
abstract::Calcineurin B-like (CBL) interacting protein kinase 15 (CIPK15) is a newly identified positive regulator which is critical to directing the O(2) deficiency signal to the sugar signaling cascade as part of Amy3D (representative Amy3 gene) regulation in rice. It is located upstream and probably contributes to reserve mo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.06.003
更新日期:2012-10-15 00:00:00
abstract::A cold-tolerant line (NY-1) and a cold-sensitive cultivar (Jinyan 4) of cucumber (Cucumis sativus) were treated with temperatures of 28 degrees C/22 degrees C or 28 degrees C/12 degrees C (day/night) in a 10-h photoperiod. Carbohydrates and related enzymes were assayed from 0 to 4 h after the start of the dark period....
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.02.005
更新日期:2007-05-01 00:00:00
abstract::Leaf water content is an important variable for understanding plant physiological properties. This study evaluates a spectral analysis approach, continuous wavelet analysis (CWA), for the spectroscopic estimation of leaf gravimetric water content (GWC, %) and determines robust spectral indicators of GWC across a wide ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.04.006
更新日期:2012-08-15 00:00:00
abstract::Cell suspension cultures of Silybum marianum are able to excrete silymarin compounds into the medium upon elicitation with methyl jasmonate or cyclodextrins. Knowledge of transport mechanism is important to understand Sm metabolism and to develop strategies aimed at increasing production by means of cell cultures. For...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.10.005
更新日期:2014-01-15 00:00:00
abstract::Endogenous free polyamines (PAs), putrescine, spermidine and spermine, from developing fruitlets of Citrus species (Citrus unshiu Marc. and Citrus clementina Hort ex Tanaka) which differ in their parthenocarpic ability, and from uniflowered leafy and leafless inflorescences differing in their ability to set, have been...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.01.011
更新日期:2005-08-01 00:00:00
abstract::The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, s...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.06.017
更新日期:2010-01-01 00:00:00
abstract::Seeds of Arabidopsis thaliana were sent to space and germinated in orbit. Seedlings grew for 4d and were then fixed in-flight with paraformaldehyde. The experiment was replicated on the ground in a Random Positioning Machine, an effective simulator of microgravity. In addition, samples from a different space experimen...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.08.012
更新日期:2010-02-15 00:00:00
abstract::The ultrastructure of mesophyll cells was studied in leaves of the Triticum aestivum L. cv. "Trizo" seedlings after two weeks of growth on soil contaminated by Pb and/or Se. The soil treatments: control; (Pb1) 50mgkg-1; (Pb2) 100mgkg-1; (Se1) 0.4mgkg-1; (Se2) 0.8mgkg-1; (Pb1+Se1); (Pb1+Se2); (P2+Se1); and (Pb2+Se2) we...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.008
更新日期:2017-12-01 00:00:00
abstract::Arbuscular mycorrhizal fungi enhance CO₂ assimilation of their hosts which ensure the demand for carbohydrates of these obligate biotrophic microorganisms. Photosynthetic parameters were measured in tomato colonised or not by the arbuscular mycorrhizal fungus Glomus mosseae. In addition, carbohydrate contents and mRNA...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.01.026
更新日期:2011-07-15 00:00:00
abstract::Plant-derived elicitor is a new type of plant vaccine developed in the contemporary era, and it has safe and broad application prospects in organic agriculture. Research on defense mechanisms triggered by elicitor has become a hot topic in recent years. The Chrysanthemum indicum polysaccharide (CIP) obtained by separa...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153107
更新日期:2020-02-01 00:00:00
abstract::Calcium oxalate crystals are a major biomineralization product in higher plants. Their biological function and use are not well understood. In this work, we focus on the isolation and crystallochemical characterization of calcium oxalate crystals from seed coats of Phaseolus vulgaris (prisms) and leaves of Vitis vinif...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-00947
更新日期:2003-03-01 00:00:00
abstract::2,4-Epibrassinolide (EBL) is a plant hormone that plays a pivotal role in regulation of plants growth and development processes under abiotic stress. The investigation was carried out to study the effect of EBL on mineral nutrients uptake and distribution with ion element analysis and X-ray microanalysis in cucumber s...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.06.010
更新日期:2015-09-01 00:00:00
abstract::Raffinose synthase (RS, EC2.4.1.82) is one of the key enzymes that channels sucrose into the raffinose family oligosaccharides (RFOs) biosynthetic pathway. However, the gene encoding RS is poorly characterized in cucumber (Cucumis sativus L.), which is a typical RFOs-translocating plant species. Here we isolated the g...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.019
更新日期:2012-12-15 00:00:00
abstract::In plant cytokinin (CK) signaling, type-B response regulators (RRs) act as major players in orchestrating the transcriptome changes in response to CK. However, their direct targets are poorly known. The identification of putative type-ARR1 motifs located within the promoter of the CK-responsive hydroxyl methyl butenyl...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.008
更新日期:2012-10-15 00:00:00
abstract::To support the key role of glutathione (GSH) in the mechanisms of tolerance and accumulation of arsenic in plants, this work examines the impact of several effectors of GSH synthesis or action in the response of maize (Zea mays L.) to arsenic. Maize was exposed in hydroponics to iso-toxic rates of 150 μM arsenate or 7...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.01.016
更新日期:2012-05-01 00:00:00
abstract::Purine and pyrimidine nucleotides participate in many biochemical processes in plants. They are building blocks for nucleic acid synthesis, an energy source, precursors for the synthesis of primary products, such as sucrose, polysaccharides, phospholipids, as well as secondary products. Therefore, biosynthesis and met...
journal_title:Journal of plant physiology
pub_type: 杂志文章,评审
doi:10.1078/0176-1617-01169
更新日期:2003-11-01 00:00:00
abstract::The effects of methyl jasmonate and jasmonic acid on galanthamine production, phenolic acid content and growth of Leucojum aestivum L. shoot culture, cultivated in submerged conditions were investigated. The best time-point for addition of elicitors was during the exponential phase of the culture growth. The maximal c...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.03.017
更新日期:2013-08-15 00:00:00
abstract::An essential step in the biosynthesis of bioactive brassinosteroids (BRs) in plants is the hydroxylation at C-22, a reaction catalyzed by P450 enzymes of the CYP90B and CYP724B subfamilies. Genes for both types of enzymes are present in many species, and in rice (Oryza sativa) and tomato (Solanum lycopersicum) both CY...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.10.013
更新日期:2012-03-01 00:00:00
abstract::Isoprene (2-methyl-1,3-butadiene) is a hemiterpene molecule. It has been estimated that the plant kingdom emits 500-750 million tons of isoprene in the environment, half of which results from tropical broadleaf trees and the remainder from shrubs. Camelina (Camelina sativa (L.) Crantz) is an emerging bioenergy plant f...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.06.005
更新日期:2017-08-01 00:00:00
abstract::Durian (Durio zibethinus) fruit was harvested at the commercially mature stage and stored at 25°C. Durian fruit have 3-5 longitudinal dehiscence zones (DZs) in the peel, which are up to 40cm long and 2cm thick in large fruit. Dehiscence started a week after harvest, was hastened by exogenous ethylene, and delayed by 1...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.04.005
更新日期:2015-06-15 00:00:00
abstract::Acidification of the starchy endosperm by the aleurone layer following germination has been established; however, the physiological and metabolic responses of this tissue to external pH have been incompletely investigated. In this investigation, isolated wheat (Triticum aestivum) aleurone layers were incubated in diff...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.05.010
更新日期:2004-12-01 00:00:00
abstract::To investigate the involvement of cytochrome P450s in the metabolism of plants treated with xenobiotic agrochemicals, bean leaves were treated with 3,5-dichlorosalicylic acid (DC-SA), a priming agent of plant defense and 2,6-dichloroisonicotinic acid (DC-INA), a chemical inducer of systemic acquired resistance. Throug...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.02.006
更新日期:2007-04-01 00:00:00
abstract::Environmental temperature change may induce modifications in membrane lipid properties and composition, which account for different physiological responses among plant species. Coffee plants, as many tropical species, are particularly sensitive to cold, but genotypes can present differences that can be exploited to im...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.07.007
更新日期:2014-02-15 00:00:00
abstract::Fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (Cucumis melo L.). In this work the presence of a class III chitinase was examined by immunological techniques in the root and stem base of a susceptible (cv. Galia) and a resistant (cv. Bredor) melon during the infe...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.02.004
更新日期:2006-01-01 00:00:00
abstract::Salicylic acid (SA) is known to affect photosynthesis under normal conditions and induces tolerance in plants to biotic and abiotic stresses through influencing physiological processes. In this study, physiological processes were compared in salt-tolerant (Pusa Vishal) and salt-sensitive (T44) cultivars of mungbean an...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.11.001
更新日期:2011-05-15 00:00:00
abstract::The Normalized Difference Vegetation Index (NDVI) is widely used for monitoring, analyzing, and mapping temporal and spatial distributions of physiological and biophysical characteristics of vegetation. It is well documented that the NDVI approaches saturation asymptotically under conditions of moderate-to-high aboveg...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01176
更新日期:2004-02-01 00:00:00
abstract::Cadmium (Cd) is a non-essential trace element and its environmental concentrations are approaching toxic levels, especially in some agricultural soils. Understanding how and where Cd is stored in plants is important for ensuring food safety. In this study, we examined two plant species that differ in the distribution ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.011
更新日期:2012-12-15 00:00:00
abstract::Increasing atmospheric CO₂ concentrations are expected to enhance plant photosynthesis and yield. Nevertheless, after long-term exposure, plants acclimate and show a reduction in photosynthetic activity (called down-regulation), which may cause a reduction in potential yield. Some authors suggest that down-regulation ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.06.015
更新日期:2010-12-15 00:00:00