Abstract：
:The microinjection of fluorescent probes into live cells is an essential component in the toolbox of modern cell biology. Microinjection techniques include the penetration of the plasma membrane and, if present, the cell wall with micropipettes, and the application of pressure or electrical currents to drive the micropipette contents into the cell. These procedures interfere with cellular functions and therefore may induce artifacts. We designed the diffusive injection micropipette (DIMP) that avoids most of the possible artifacts due to the drastically reduced volume of its fluid contents and the utilization of diffusion for cargo delivery into the target cell. DIMPs were successfully tested in plant, fungal, and animal cells. Using the continuity of cytoplasmic dynamics over ten minutes after impalement of Nicotiana trichome cells as a criterion for non-invasiveness, we found DIMPs significantly less disruptive than conventional pressure microinjection. The design of DIMPs abolishes major sources of artifacts that cannot be avoided by other microinjection techniques. Moreover, DIMPs are inexpensive, easy to produce, and can be applied without specific equipment other than a micromanipulator. With these features, DIMPs may become the tool of choice for studies that require the least invasive delivery possible of materials into live cells.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Howell AH,Peters WS,Knoblauch Mdoi
10.1016/j.jplph.2019.153060subject
Has Abstractpub_date
2020-01-01 00:00:00pages
153060eissn
0176-1617issn
1618-1328pii
S0176-1617(19)30188-9journal_volume
244pub_type
杂志文章abstract：:We previously isolated Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) from tobacco leaves. The NMMP1 gene encodes a highly conserved, Zn-containing catalytic protease domain that functions as a factor in the plant's defense against bacterial pathogens. Expression of NMMP1 was strongly induced during interactio...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.08.010
更新日期：2017-11-01 00:00:00
abstract：:Low temperature is one of the primary causes of economic loss in agricultural production, and in this regard, expansin proteins are known to play important roles in plant growth and responses to various abiotic stresses and plant hormones. In order to elucidate the roles of expansin genes in the response of Dongnongdo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153004
更新日期：2019-09-01 00:00:00
abstract：:Rice (Oryza sativa L.) ssp. indica is the most cultivated species in the South of Brazil. However, these plants face low temperature stress from September to November, which is the period of early sowing, affecting plant development during the initial stages of growth, and reducing rice productivity. This study aimed ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2020.153307
更新日期：2020-12-01 00:00:00
abstract：:Measurements of chlorophyll a fluorescence, nutrient and trace elements, total protein content and malonyldialdehyde in leaves of Arabidopsis thaliana between 1 and 192 h after treatment with 0, 1 or 3 mM 2-3H-benzoxazolinone (BOA), together with imaging of chlorophyll a fluorescence and of the distributions of hydrog...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.11.011
更新日期：2011-06-15 00:00:00
abstract：:Plants respond to different environmental cues in a complex way, entailing changes at the cellular and physiological levels. An important step to understand the molecular foundation of stress response in plants is the analysis of stress-responsive proteins. In this work we attempted to investigate and compare changes ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.10.020
更新日期：2014-03-15 00:00:00
abstract：:We investigated the effects of short-term salinity stress and spermidine application to salinized nutrient solution on polyamine metabolism and various stress defense reactions in the roots of two cucumber (Cucumis sativus L.) cultivars, Changchun mici and Jinchun No. 2. Seedlings grown in nutrient solution salinized ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.11.006
更新日期：2008-10-09 00:00:00
abstract：:After several years of close contacts and extensive discussion between various plant physiologists of different European countries, the Federation of European Societies of Plant Physiology (FESPP) was established in 1978 in Edinburgh. The aim of the FESPP was and remains to promote up-to-date plant physiology research...
journal_title：Journal of plant physiology
pub_type： 历史文章,杂志文章
doi：10.1078/0176-1617-01371
更新日期：2004-06-01 00:00:00
abstract：:Aquaporins mediate the movement of water across cell membranes. Plasma membrane intrinsic protein 2;5 from Populus trichocarpa×deltoides (PtdPIP2;5) was previously demonstrated to be a functionally important water conducting aquaporin. To study the relevance of aquaporin-mediated root water transport at low temperatur...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.02.001
更新日期：2016-03-15 00:00:00
abstract：:Tungsten is a heavy metal with increasing concern over its environmental impact. In plants it is extensively used to deplete nitric oxide by inhibiting nitrate reductase, but its presumed toxicity as a heavy metal has been less explored. Accordingly, its effects on Arabidopsis thaliana primary root were assessed. The ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2014.04.010
更新日期：2014-08-15 00:00:00
abstract：:In our previous study we showed that volatile organic compounds (VOCs) from Alcaligenes faecalis JBCS1294 (JBCS1294) induced tolerance to salt stress in Arabidopsis thaliana by influencing the auxin and gibberellin pathways and upregulating the expression of key ion transporters. The aim of this study was to evaluate ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.04.002
更新日期：2017-07-01 00:00:00
abstract：:Cinnamoyl CoA reductase catalyzes the reduction of cinnamic acid CoA esters into their corresponding aldehydes, the first step of the phenylpropanoid pathway specifically dedicated to monolignol biosynthesis. Two cDNA clones encoding cinnamoyl CoA reductase (CCR) have been isolated from Hordeum vulgare (HvCCR) and Sol...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01074
更新日期：2004-01-01 00:00:00
abstract：:This study investigates the influence of various rootstocks for pear on the phytochemical composition in the phloem above and below the graft union and the role of phenols in pear graft incompatibility. Assays of phloem with cambium from 4-year-old 'Conference', 'Abate Fetel' and 'Williams' pear trees grafted on diffe...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.10.022
更新日期：2014-03-01 00:00:00
abstract：:The plant secondary metabolism generates numerous compounds harbouring pharmaceutical activity. In plants, these compounds are typically formed by different and specialised cell types that have to interact constituting a metabolic process chain. This interactivity impedes biotechnological production of secondary compo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.06.004
更新日期：2016-08-01 00:00:00
abstract：:No significant changes were detected in the proportions of the various monosaccharides present in the cell wall polysaccharides of the peripheral cell layers on the upper and lower sides of sunflower hypocotyls which had undergone gravicurvature. The incorporation of radioactive glucose into the major classes of polys...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/S0176-1617(85)80164-4
更新日期：1985-02-01 00:00:00
abstract：:The regulation of gene expression is a key factor in plant acclimation to stress, and it is thought that manipulation of the expression of critical stress-responsive genes should ultimately provide increased protection against abiotic stress. The aim of this study was to test the hypothesis that the ectopic expression...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.04.007
更新日期：2012-09-01 00:00:00
abstract：:A metabolomics approach using (1)H NMR and GC-MS profiling of primary metabolites and quantification of adenine nucleotides with luciferin bioluminescence was employed to investigate the spatial changes of metabolism in melon fruit. Direct (1)H NMR profiling of juice collected from different locations in the fruit fle...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.08.010
更新日期：2010-02-15 00:00:00
abstract：:Researchers have proven that nanomaterials have a significant effect on plant growth and development. To better understand the effects of nanomaterials on plants, Zhongshuang 11 was treated with different concentrations of graphene oxide. The results indicated that 25-100mg/l graphene oxide treatment resulted in short...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.02.011
更新日期：2016-04-01 00:00:00
abstract：:Most of our knowledge on the regulation of photosynthesis originates from studies performed in highly controlled laboratory conditions. However, in their natural habitats plants are simultaneously subjected to a broad range of abiotic and biotic stimuli which influence photosynthetic efficiency; hence there is an emer...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.11.016
更新日期：2013-04-15 00:00:00
abstract：:The leaf is considered the most important vegetative organ of tank epiphytic bromeliads due to its ability to absorb and assimilate nutrients. However, little is known about the physiological characteristics of nutrient uptake and assimilation. In order to better understand the mechanisms utilized by some tank epiphyt...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.01.008
更新日期：2011-07-15 00:00:00
abstract：:Type-A response regulators play an important role in cytokinin-induced adventitious shoot formation, acting as negative regulators of cytokinin signal transduction. In this work, we obtained the full-length cDNA clone of a type-A response regulator from the conifer Pinus pinea, designated PipiRR1. The derived peptide ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.02.012
更新日期：2010-08-15 00:00:00
abstract：:Changes in phenolics (PhC - phenolic compounds) measured as UV-absorbing compounds (UVAC) and their localization as well as growth, lipid peroxidation (TBARS level) and proline (Pro) level in three-day-old roots of seedlings (T(o) stage) obtained from hydroprimed (H) and hydroprimed with melatonin (H-MEL) seeds after ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.08.011
更新日期：2012-01-01 00:00:00
abstract：:pea-MADS4 (PEAM4) regulates floral morphology in Pisum sativum L., however, its molecular mechanisms still remain unclear. Virus-induced gene silencing (VIGS) is a recently developed reverse genetic approach that facilities an easier and more rapid study of gene functions. In this study, the PEAM4 gene was effectively...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.08.010
更新日期：2014-01-15 00:00:00
abstract：:We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomat...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.04.006
更新日期：2008-06-16 00:00:00
abstract：:In the current study, we investigated the influences of theobroxide on stem elongation in spinach (Spinacia oleracea). Our results showed that stem elongation and flower formation were inhibited by spraying spinach plants with theobroxide under inductive, long day conditions (16 h light/8 h dark), while application of...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.05.005
更新日期：2006-03-01 00:00:00
abstract：:The limiting steps of ethylene-dependent (methylsulfanyl)alkanoate ester biosynthesis have been investigated in this study, using closely related Actinidia chinensis genotypes and the commercial cultivar 'Hort16A'. Quantification of methylsulfanyl-compounds from the headspace of ethylene-producing kiwifruits revealed ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.10.001
更新日期：2011-05-01 00:00:00
abstract：:Pleiotropic drug resistant (PDR/ABCG) genes are involved in plant response to biotic and abiotic stresses. In this work, we cloned, from Solanum tuberosum, four PDR/ABCG transporter genes named StPDR1, StPDR2, StPDR3 and StPDR4, which were differentially expressed in plant tissues and cell cultures. A number of differ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.07.008
更新日期：2011-12-15 00:00:00
abstract：:MicroRNAs (miRNAs) regulate target gene expression by mediating target gene cleavage or inhibition of translation at transcriptional and post-transcriptional levels in higher plants. Until now, many grapevine microRNAs (Vv-miRNAs) have been identified and quite a number of miRNA target genes were also verified by vari...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.02.005
更新日期：2013-07-01 00:00:00
abstract：:Characterization of the photosynthetic electron transport in a mutant of Spirulina platensis, generated by chemical mutagenesis, demonstrated that the electron transfer from the plastoquinone (PQ) to cytochrome b6/f was slowed. Thermoluminescence (TL) measurements suggested the presence of reversed energy flow via PQ,...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.11.012
更新日期：2005-10-01 00:00:00
abstract：:Currents flowing through slow vacuolar SV channels of rape (Brassica napus) growing on media supplemented with Cd²+ (400 μM), and/or SeO₄(²⁻) (2μM) were examined. The aim of the study was to investigate the role of Cd²+ in modulation of SV channel activity and to determine whether Se reverses the effect of cadmium. Va...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.06.016
更新日期：2010-12-15 00:00:00
abstract：:Purine and pyrimidine nucleotides participate in many biochemical processes in plants. They are building blocks for nucleic acid synthesis, an energy source, precursors for the synthesis of primary products, such as sucrose, polysaccharides, phospholipids, as well as secondary products. Therefore, biosynthesis and met...
journal_title：Journal of plant physiology
pub_type： 杂志文章,评审
doi：10.1078/0176-1617-01169
更新日期：2003-11-01 00:00:00
