Abstract：
:The plant secondary metabolism generates numerous compounds harbouring pharmaceutical activity. In plants, these compounds are typically formed by different and specialised cell types that have to interact constituting a metabolic process chain. This interactivity impedes biotechnological production of secondary compounds, because cell differentiation is suppressed under the conditions of a batch bio-fermenter. We present a novel strategy to address this limitation using a biomimetic approach, where we simulate the situation in a real tissue by a microfluidic chamber system, where plant cells can be integrated into a process flow. We show that walled cells of the plant model tobacco BY-2 can be successfully cultivated in this system and that physiological parameters (such as cell viability, mitotic index and division synchrony) can be preserved over several days. The microfluidic design allows to resolve dynamic changes of specific metabolites over different stages of culture development. These results serve as proof-of-principle that a microfluidic organisation of cultivated plant cells can mimic the metabolic flows in a real plant tissue.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Maisch J,Kreppenhofer K,Büchler S,Merle C,Sobich S,Görling B,Luy B,Ahrens R,Guber AE,Nick Pdoi
10.1016/j.jplph.2016.06.004subject
Has Abstractpub_date
2016-08-01 00:00:00pages
28-34eissn
0176-1617issn
1618-1328pii
S0176-1617(16)30097-9journal_volume
200pub_type
杂志文章abstract：:In rose flowers, 2-phenylethanol (2PE) is biosynthesized from l-phenylalanine (l-Phe) via phenylacetaldehyde (PAld) by the actions of two enzymes, pyridoxal-5'-phosphate (PLP)-dependent aromatic amino acid decarboxylase (AADC) and phenylacetaldehyde reductase (PAR). We here report that Rosa 'Yves Piaget' aromatic amin...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.12.005
更新日期：2012-03-15 00:00:00
abstract：:This report demonstrates that mitochondria isolated from thermogenic Arum spadices possess an ATP-sensitive potassium channel--responsible for electrical potential (DeltaPsi) collapse and mitochondrial swelling--whose characteristics are similar to those previously described in pea and wheat mitochondria. In order to ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.10.012
更新日期：2008-09-08 00:00:00
abstract：:The purification of 6-O(4-O)-indole-3-ylacetyl-beta-D-glucose (IAGlc) hydrolase from immature kernels of maize (Zea mays) was undertaken to separate this enzyme from 1-O-IAGlc hydrolase and beta-glucosidase. Partially purified 6-O(4-O)-IAGlc hydrolase was found to be the specific enzyme catalyzing hydrolysis of stable...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.05.015
更新日期：2005-02-01 00:00:00
abstract：:The effect of subculture interval and total culture age on plant regeneration from serially subcultured, leaf derived embryogenic callus cultures of Pennisetum purpureum (Napier grass) was determined. During 38 weeks of culture both embryogenic callus proliferation and the yield of plants was maximal for callus from f...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/S0176-1617(84)80028-0
更新日期：1984-12-01 00:00:00
abstract：:Ion-selective microelectrodes are a powerful tool in studying adaptive responses of plant cells and tissues to various abiotic stresses. However, application of this technique in Na(+) flux measurements was limited due to poor selectivity for Na(+) ions of commercially available Na(+) cocktails. Often, these cocktails...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.12.006
更新日期：2011-07-01 00:00:00
abstract：:Smut infection alters the transcription of dirigent proteins (DIR) by sugarcane plants. Here, we show that these alterations are associated to an elevated production of cytotoxic lignans. Smut-resistant sugarcane varieties display a fivefold increase in pinoresinol and also produce elevated amounts of secoisolaricires...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153087
更新日期：2020-01-01 00:00:00
abstract：:Ascorbate is a major antioxidant and radical scavenger in plants. Monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR) are two enzymes of the ascorbate-glutathione cycle that maintain ascorbate in its reduced state. MDAR2 (At3g09940) and DHAR5 (At1g19570) expression was upregulated in the roots ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.12.016
更新日期：2009-08-15 00:00:00
abstract：:This study has been carried out to investigate the photosynthetic activities in developing embryos of Sesbania sesban under a highly osmotic environment. In S. sesban, the embryo turns green/chlorophyllous at the early heart shape stage. Interestingly, despite being deeply embedded within the supporting tissues (sever...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.03.002
更新日期：2004-10-01 00:00:00
abstract：:Plant growth promoting rhizobacteria (PGPR) are extensively used as biofertilizers to improve the soil nutrition for a variety of crop plants. The plant-PGPR interaction, with special reference to chemical signalling molecules is not understood clearly, unlike other beneficial plant-microbe interactions. Chemo-attract...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153057
更新日期：2019-12-01 00:00:00
abstract：:The distribution of the endogenous PA fractions throughout the entire perennial woody grapevine (Vitis vinifera L.) plant was studied, along with the expression profiles of the PA anabolic and catabolic genes and their substrates and secondary metabolites. Putrescine fractions increased with increasing leaf age, altho...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.03.013
更新日期：2009-09-15 00:00:00
abstract：:The relationship between coleoptile elongation and ethanolic fermentation was investigated in rice (Oryza sativa L.) coleoptiles of four cultivars subjected to a 48-h anoxic stress. The coleoptile elongation of all cultivars was suppressed by anoxic stress; however, the elongation of cvs Yukihikari and Nipponbare was ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.09.017
更新日期：2007-02-01 00:00:00
abstract：:Fur proteins are global regulators present in all prokaryotes. In Anabaena sp. PCC 7120 FurA controls iron uptake and modulates an important set of genes related primarily to photosynthesis, nitrogen metabolism and oxidative stress defense. Expression of furA is tuned by the cis-acting antisense alpha-furA RNA that is...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.10.009
更新日期：2010-04-15 00:00:00
abstract：:Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a "dry-down" experiment under c...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.12.014
更新日期：2014-04-15 00:00:00
abstract：:Environmental constraints disturb plant metabolism and are often associated with photosynthetic impairments and yield reductions. Among them, low positive temperatures are of up most importance in tropical plant species, namely in Coffea spp. in which some acclimation ability has been reported. To further explain cold...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.11.013
更新日期：2011-05-15 00:00:00
abstract：:Although activation of A-type cyclin-dependent kinase (CDKA) is required for plant cell division, little is known about how CDKA is activated before commitment to cell division. Here, we show that auxin is required for the formation of active CDKA-associated complexes, promoting assembly of the complex in tobacco susp...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.01.005
更新日期：2007-09-01 00:00:00
abstract：:Drought is one of the stresses that limit the yield of barley. Despite extensive studies focused on the issue, the molecular mechanism of the response to drought is still not fully understood. In our previous study, we proposed drought-induced signal perception controlled by actin filaments (AFs). To test this hypothe...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.02.006
更新日期：2016-04-01 00:00:00
abstract：:Oxidative stress and ultrastructural changes under hexavalent chromium stress were investigated in developing rice seedlings. Chromium treatment for 24 or 48h resulted in inhibition of root length and dry biomass. Atomic absorption spectrometry analysis of roots showed that chromium accumulation increased with increas...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.01.012
更新日期：2007-11-01 00:00:00
abstract：:The effects of high salinity (0-400 mmol/L NaCl) on photosystem II (PSII) photochemistry and photosynthetic pigment composition were investigated in the halophyte Artimisia anethifolia grown under outdoor conditions and exposed to full sunlight. High salinity resulted in an inhibition in plant growth and a significant...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-00839
更新日期：2003-04-01 00:00:00
abstract：:Measurements of chlorophyll a fluorescence, nutrient and trace elements, total protein content and malonyldialdehyde in leaves of Arabidopsis thaliana between 1 and 192 h after treatment with 0, 1 or 3 mM 2-3H-benzoxazolinone (BOA), together with imaging of chlorophyll a fluorescence and of the distributions of hydrog...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.11.011
更新日期：2011-06-15 00:00:00
abstract：:Soil flooding has been widely reported to affect large areas of the world. In this work, we investigated the effect of waterlogging on citrus carbon and nitrogen pools and partitioning. Influence on their uptake and translocation was also studied through ¹⁵N and ¹³C labeling to provide insight into the physiological m...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.03.016
更新日期：2012-08-15 00:00:00
abstract：:The expression of foreign phytoalexins in a new host is thought to increase fungal resistance, since host-specific pathogens have not experienced selection for detoxifying or metabolising the novel antifungal compounds. Two resveratrol synthase genes vst1 and vst2 from grapevine (Vitis vinifera L.) and the pinosylvin ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.11.005
更新日期：2005-09-01 00:00:00
abstract：:A balanced nutrient supply is a critical factor affecting accumulation of terpenoids in plants, yet data related to the interactive effects of two essential nutrients for the biosynthesis of sesquiterpenes are scarce. Here, the interactional effects between magnesium (Mg) and manganese (Mn) on plant growth, oxidative ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.06.017
更新日期：2016-09-01 00:00:00
abstract：:Ascorbic acid oxidase (AAO) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. In spite of extensive molecular characterization the physiological role of AAO is still uncertain. Abundant mRNA, protein and activity of AAO were observed in illuminated leaves ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.09.016
更新日期：2007-01-01 00:00:00
abstract：:A cold-tolerant line (NY-1) and a cold-sensitive cultivar (Jinyan 4) of cucumber (Cucumis sativus) were treated with temperatures of 28 degrees C/22 degrees C or 28 degrees C/12 degrees C (day/night) in a 10-h photoperiod. Carbohydrates and related enzymes were assayed from 0 to 4 h after the start of the dark period....
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.02.005
更新日期：2007-05-01 00:00:00
abstract：:Common sage (Salvia officinalis L., Lamiaceae) is one of the most important medicinal and aromatic plants, with antioxidant, antimicrobial, spasmolytic, astringent, antihidrotic and specific sensorial properties. The essential oil of the plant, composed mainly of the monoterpenes 1,8-cineole, alpha-thujone, beta-thujo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.12.009
更新日期：2010-07-01 00:00:00
abstract：:The effects of oxygen supply within the range 20.8-50% (using pure oxygen and air), on cell cultures of Panax ginseng were investigated in a balloon-type bubble bioreactor (5L capacity, containing 4 L Murashige and Skoog medium, supplemented with 7.0 mgL(-1) indolebutyric acid, 0.5 mgL(-1) kinetin and 30 gL(-1) sucros...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.08.014
更新日期：2006-12-01 00:00:00
abstract：:1-Deoxy-d-xylulose 5-phosphate reductoisomerase (DXR, EC: 1.1.1.267) is the second enzyme in the 2C-methyl-d-erythritol 4-phosphate (MEP) pathway, one of the two pathways in plants that can produce isoprenoids. The MEP pathway is the source of isoprene emitted from leaves, but rubber production is believed to result p...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.06.014
更新日期：2008-06-16 00:00:00
abstract：:The aim of the present work was to analyze the impact of salinity on the plant response to Cd toxicity in the Mediterranean halophyte species Inula crithmoides. For this purpose, cuttings were cultivated hydroponically during 21d in the presence of 0, 25 or 50μM CdCl2 combined or not with 0, 100, 200 and 400mM NaCl. T...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.05.018
更新日期：2017-09-01 00:00:00
abstract：:Climate change is submitting countries of the Mediterranean arc to periods of drought alternating with heavy rain and waterlogging. Eventual floods along with the rising CO2 in the atmosphere present an unpredictable scenario that affects crop survival. The effect of both stresses combined has been studied in sweet ch...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.03.011
更新日期：2017-06-01 00:00:00
abstract：:A non-destructive nitrogen (N) detector [Agriexpert PPW-3000 (PPW-3000)] is a useful device for rapid and non-destructive measurement of leaf N content. However, some studies find a poor correlation between the PPW-3000 reading and the actual leaf N content; the R2 value of the approximate equation was low. To improve...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.09.004
更新日期：2006-04-01 00:00:00
