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Effect of oxygen supply on cell growth and saponin production in bioreactor cultures of Panax ginseng.

Abstract：

:The effects of oxygen supply within the range 20.8-50% (using pure oxygen and air), on cell cultures of Panax ginseng were investigated in a balloon-type bubble bioreactor (5L capacity, containing 4 L Murashige and Skoog medium, supplemented with 7.0 mgL(-1) indolebutyric acid, 0.5 mgL(-1) kinetin and 30 gL(-1) sucrose). A 40% oxygen supply was found to be optimal for the production of both cell mass and saponin yielding values of 12.8 g(DW)L(-1), 4.5mg(gDW)(-1) on day 25, respectively. Low (20.8%, 30%) and high (50%) oxygen concentration supplies were unfavorable to cell growth and saponin accumulation. The results indicate that oxygen supplementation to bioreactor-based ginseng cultures was beneficial for biomass accumulation and saponin production.

journal_name

J Plant Physiol

journal_title

Journal of plant physiology

authors

Thanh NT,Murthy HN,Yu KW,Seung Jeong C,Hahn EJ,Paek KY

doi

10.1016/j.jplph.2005.08.014

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

1337-41

issue

12

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(05)00345-7

journal_volume

163

pub_type

杂志文章

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