Abstract:
:The expression of foreign phytoalexins in a new host is thought to increase fungal resistance, since host-specific pathogens have not experienced selection for detoxifying or metabolising the novel antifungal compounds. Two resveratrol synthase genes vst1 and vst2 from grapevine (Vitis vinifera L.) and the pinosylvin synthase gene pss from pine (Pinus sylvestris L.) were stably transformed into bread wheat. The expression of the target genes is regulated by stress-inducible grapevine promoters. The vst1 and vst2 promoters were functional in wheat and retained their expression profiles described for grapevine. ALL vst and pss transgenic lines accumulated stilbene derivatives upon induction by UV light. The detected stilbenes showed a remarkable similarity to resveratrol and pinosylvin, however were found to be more hydrophilic than resveratrol and pinosylvin. Upon inoculation with the biotrophic pathogen Puccinia recondita f.sp. tritici several vst expressing wheat lines showed a significant reduction of disease symptoms (19 +/- 9% to 27 +/- 8%) compared to wild-type plants. The reduction of disease symptoms was even more obvious after inoculation with the facultative biotrophic pathogen Septoria nodorum Berk. and ranged from 42 +/- 13% to 71 +/- 4%. None of the four tested pss expressing lines showed a reduction in disease incidence.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Serazetdinova L,Oldach KH,Lörz Hdoi
10.1016/j.jplph.2004.11.005subject
Has Abstractpub_date
2005-09-01 00:00:00pages
985-1002issue
9eissn
0176-1617issn
1618-1328pii
S0176-1617(05)00011-8journal_volume
162pub_type
杂志文章abstract::MicroRNAs (miRNAs) regulate target gene expression by mediating target gene cleavage or inhibition of translation at transcriptional and post-transcriptional levels in higher plants. Until now, many grapevine microRNAs (Vv-miRNAs) have been identified and quite a number of miRNA target genes were also verified by vari...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.02.005
更新日期:2013-07-01 00:00:00
abstract::Plant WRKY transcription factors play a vital role in abiotic stress tolerance and regulation of plant defense responses. This study examined AtWRKY11 and AtWRKY17 expression under ABA, salt, and osmotic stress at different developmental stages in Arabidopsis. We used reverse transcriptase PCR, quantitative real-time ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.04.007
更新日期:2018-07-01 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) constitute a unique family of enzymes in plants that are characterized by a C-terminal calmodulin (CaM)-like domain. Through protein kinase assays, we have examined the levels of cucumber calcium-dependent kinase (CsCDPK) activity in various organs of cucumber seedlings and pl...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2003.11.010
更新日期:2004-08-01 00:00:00
abstract::Environmental temperature change may induce modifications in membrane lipid properties and composition, which account for different physiological responses among plant species. Coffee plants, as many tropical species, are particularly sensitive to cold, but genotypes can present differences that can be exploited to im...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.07.007
更新日期:2014-02-15 00:00:00
abstract::We present here the comparative protective potentiality of exogenously applied polyamines (PAs), namely spermidine (Spd) and spermine (Spm), in mitigating NaCl toxicity and inducing short-term salinity tolerance in three indica rice varieties, namely M-1-48 (salt-sensitive), Nonabokra (salt-tolerant) and Gobindobhog (...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.07.009
更新日期:2011-03-01 00:00:00
abstract::Xanthomonas albilineans is the causal organism of leaf scald, a bacterial vascular disease of sugarcane. Xanthomonas may invade the parenchyma between the bundles and cause reddened pockets of gum, identified as a xanthan-like polysaccharide. Since xanthan contains glucuronic acid, the ability of Xanthomonas to produc...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.03.008
更新日期:2008-03-13 00:00:00
abstract::Rice (Oryza sativa L.) ssp. indica is the most cultivated species in the South of Brazil. However, these plants face low temperature stress from September to November, which is the period of early sowing, affecting plant development during the initial stages of growth, and reducing rice productivity. This study aimed ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153307
更新日期:2020-12-01 00:00:00
abstract::Fruit ripening is a complex process involving many physiological and biochemical changes, including those for ethylene, carotenoid, and cell wall metabolism. Tomato (Solanum lycopersicum) serves as a research model for fruit development and ripening because it possesses numerous favorable genetic features. In this stu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.01.014
更新日期:2016-04-01 00:00:00
abstract::Ascorbic acid oxidase (AAO) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. In spite of extensive molecular characterization the physiological role of AAO is still uncertain. Abundant mRNA, protein and activity of AAO were observed in illuminated leaves ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.09.016
更新日期:2007-01-01 00:00:00
abstract::Plants are able to acclimate to highly fluctuating light environment and evolved a short- and long-term light acclimatory responses, that are dependent on chloroplasts retrograde signalling. In this review we summarise recent evidences suggesting that the chloroplasts act as key sensors of light intensity changes in a...
journal_title:Journal of plant physiology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.jplph.2013.06.005
更新日期:2013-11-15 00:00:00
abstract::We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomat...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.04.006
更新日期:2008-06-16 00:00:00
abstract::The effect of five topolins (meta-Topolin=mT; meta-Topolin riboside=mTR; meta-Methoxy topolin=MemT; meta-Methoxy topolin riboside=MemTR and 6-(meta-methoxy)-9-(tetrahydropyran-2-yl)-topolin=MemTTHP) on the photosynthetic pigments and leaf structures of micropropagated 'Williams' bananas was compared with the commonly ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.06.006
更新日期:2012-10-15 00:00:00
abstract::In intact vegetative plants of N. tabacum grown with progressively rising supplies of potassium, the increase in concentration of the cation is greater in the basal region, and growth is promoted in leaves approaching their final weight and subtending basal nodes while it is restricted in the apical leaves and interno...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(85)80046-8
更新日期:1985-01-01 00:00:00
abstract::Seeds of Arabidopsis thaliana were sent to space and germinated in orbit. Seedlings grew for 4d and were then fixed in-flight with paraformaldehyde. The experiment was replicated on the ground in a Random Positioning Machine, an effective simulator of microgravity. In addition, samples from a different space experimen...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.08.012
更新日期:2010-02-15 00:00:00
abstract::Ascorbate is a major antioxidant and radical scavenger in plants. Monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR) are two enzymes of the ascorbate-glutathione cycle that maintain ascorbate in its reduced state. MDAR2 (At3g09940) and DHAR5 (At1g19570) expression was upregulated in the roots ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.12.016
更新日期:2009-08-15 00:00:00
abstract::The ultrastructure of mesophyll cells was studied in leaves of the Triticum aestivum L. cv. "Trizo" seedlings after two weeks of growth on soil contaminated by Pb and/or Se. The soil treatments: control; (Pb1) 50mgkg-1; (Pb2) 100mgkg-1; (Se1) 0.4mgkg-1; (Se2) 0.8mgkg-1; (Pb1+Se1); (Pb1+Se2); (P2+Se1); and (Pb2+Se2) we...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.008
更新日期:2017-12-01 00:00:00
abstract::Nitric oxide (NO) is a highly reactive, membrane-permeable free radical, which has recently emerged as an important antioxidant. Here we investigated the protective effect of NO against the toxicity and NH4+ accumulation in rice leaves caused by excess CuSO4 (10mmol L(-1)). It was found that free radical scavengers (s...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.02.003
更新日期:2005-12-01 00:00:00
abstract::Chromium (Cr) contamination in soil is a growing concern in relation to sustainable agricultural production and food safety. Nitric oxide (NO) and, more recently, hydrogen sulfide (H2S) are considered to be new signalling molecules with biotechnological applications in the agronomical sector. Using 9-day-old maize (Ze...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.010
更新日期:2017-12-01 00:00:00
abstract::Isozymes of UGPase with unique catalytic properties were purified from the cold-induced-sweetening (CIS) resistant cultivar Snowden (Solanum tuberosum). Two distinct peaks of UGPase activity were obtained when protein extracts were subjected to anion-exchange chromatography on DEAE-Sephacel. Polypeptides in the first ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01045
更新日期:2003-06-01 00:00:00
abstract::The flavor and taste of fruits are often determined by terpenes. We identified three cDNAs encoding putative terpene synthases from olive fruits of cv. Frantoio and Grignano. Heterologous expression in a bacterial system demonstrated that one of the terpene synthases, OeGES1, was an active monoterpene synthase that co...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.01.021
更新日期:2012-06-15 00:00:00
abstract::The application of nitrification inhibitors (NIs) together with nitrogen fertilizers in grasslands is an effective alternative to reduce nitrate leaching and nitrogenous gases emissions to the atmosphere. Nevertheless, the use of NIs increases the amount of ammonium available for the plant that, due to its reported to...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.11.013
更新日期:2007-12-01 00:00:00
abstract::The interactive effects of salinity and potassium deficiency on the growth, mineral elements and photosynthetic performance were investigated in wild (Hordeum maritimum L.) and cultivated barley (Hordeum vulgare L. var. Manel). At 28 d of growth, plants were treated with 3 mM K and 0 mM NaCl (3-0); 3 mM K and 100 mM N...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.06.013
更新日期:2009-12-15 00:00:00
abstract::Super-growing roots (superroots; SR), which have been established in the legume species Lotus corniculatus, are a fast-growing root culture that allows continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely growth regulator-free culture conditions. These features are uniqu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.03.003
更新日期:2008-08-25 00:00:00
abstract::Glycolate oxidase (EC 1.1.3.15) activity was detected both in the bundle sheath (79%) and mesophyll (21%) tissues of maize leaves. Three peaks of glycolate oxidase activity were separated from maize leaves by the linear KCl gradient elution from the DEAE-Toyopearl column. The first peak corresponded to the glycolate o...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01105
更新日期:2003-08-01 00:00:00
abstract::The effects of different doses of NaCl on the expression profiles of genes involved in the mitochondrial electron transport chain (miETC), H2O2 and O2- levels, and antioxidant enzymes and amino acid metabolism were investigated in the leaves of poplar (Populus deltoides x Populus euramericana 'Nanlin 895'). In the miE...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153017
更新日期:2019-09-01 00:00:00
abstract::Cysteine synthesis in plants represents the final step of assimilatory sulfate reduction and the almost exclusive entry reaction of reduced sulfur into metabolism not only of plants, but also the human food chain in general. It is accomplished by the sequential reaction of two enzymes, serine acetyltransferase (SAT) a...
journal_title:Journal of plant physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jplph.2005.11.013
更新日期:2006-02-01 00:00:00
abstract::The plant secondary metabolism generates numerous compounds harbouring pharmaceutical activity. In plants, these compounds are typically formed by different and specialised cell types that have to interact constituting a metabolic process chain. This interactivity impedes biotechnological production of secondary compo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.06.004
更新日期:2016-08-01 00:00:00
abstract::Over the last years, the discovery of various natural and the development of a row of engineered CRISPR/Cas nucleases have made almost every site of plant genomes accessible for the induction of specific changes. Newly developed tools open up a wide range of possibilities for the induction of genetic variability, from...
journal_title:Journal of plant physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jplph.2020.153332
更新日期:2020-12-15 00:00:00
abstract::Plants release volatiles to communicate with each other and to attract or repel insects. The methods used to collect volatiles are varied. Here, we describe a simple solvent-less, solid phase microextraction-based method to collect the volatiles released from intact citrus leaves. We were able to collect up to 39 vola...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.11.001
更新日期:2017-01-01 00:00:00
abstract::The regulation of gene expression is a key factor in plant acclimation to stress, and it is thought that manipulation of the expression of critical stress-responsive genes should ultimately provide increased protection against abiotic stress. The aim of this study was to test the hypothesis that the ectopic expression...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.04.007
更新日期:2012-09-01 00:00:00