Heterologous expression and biochemical characterization of two calcium-dependent protein kinase isoforms CaCPK1 and CaCPK2 from chickpea.

Abstract:

:In plants, calcium-dependent protein kinases (CPKs) constitute a unique family of enzymes consisting of a protein kinase catalytic domain fused to carboxy-terminal autoregulatory and calmodulin-like domains. We isolated two cDNAs encoding calcium-dependent protein kinase isoforms (CaCPK1 and CaCPK2) from chickpea. Both isoforms were expressed as fusion proteins in Escherichia coli. Biochemical analyses have identified CaCPK1 and CaCPK2 as Ca(2+)-dependent protein kinases since both enzymes phosphorylated themselves and histone III-S as substrate only in the presence of Ca(2+). The kinase activity of the recombinant enzymes was calmodulin independent and sensitive to CaM antagonists W7 [N-(6-aminohexyl)-5-chloro-1-naphthalene sulphonamide] and calmidazoilum. Phosphoamino acid analysis revealed that the isoforms transferred the gamma-phosphate of ATP only to serine residues of histone III-S and their autophosphorylation occurred on serine and threonine residues. These two isoforms showed considerable variations with respect to their biochemical and kinetic properties including Ca(2+) sensitivities. The recombinant CaCPK1 has a pH and temperature optimum of pH 6.8-8.6 and 35-42 degrees C, respectively, whereas CaCPK2 has a pH and temperature optimum of pH 7.2-9 and 35-42 degrees C, respectively. Taken together, our results suggest that CaCPK1 and CaCPK2 are functional serine/threonine kinases and may play different roles in Ca(2+)-mediated signaling in chickpea plants.

journal_name

J Plant Physiol

authors

Syam Prakash SR,Jayabaskaran C

doi

10.1016/j.jplph.2006.04.005

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

1083-93

issue

11

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(06)00134-9

journal_volume

163

pub_type

杂志文章
  • Cysteine and methionine contribute differentially to regulate alternative oxidase in leaves of poplar (Populus deltoides x Populus euramericana 'Nanlin 895') seedlings exposed to different salinity.

    abstract::The effects of different doses of NaCl on the expression profiles of genes involved in the mitochondrial electron transport chain (miETC), H2O2 and O2- levels, and antioxidant enzymes and amino acid metabolism were investigated in the leaves of poplar (Populus deltoides x Populus euramericana 'Nanlin 895'). In the miE...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2019.153017

    authors: Liao Y,Cui R,Yuan T,Xie Y,Gao Y

    更新日期:2019-09-01 00:00:00

  • Photochemical processes, carbon assimilation and RNA accumulation of sucrose transporter genes in tomato arbuscular mycorrhiza.

    abstract::Arbuscular mycorrhizal fungi enhance CO₂ assimilation of their hosts which ensure the demand for carbohydrates of these obligate biotrophic microorganisms. Photosynthetic parameters were measured in tomato colonised or not by the arbuscular mycorrhizal fungus Glomus mosseae. In addition, carbohydrate contents and mRNA...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2011.01.026

    authors: Boldt K,Pörs Y,Haupt B,Bitterlich M,Kühn C,Grimm B,Franken P

    更新日期:2011-07-15 00:00:00

  • Blue light differentially represses mesophyll conductance in high vs low latitude genotypes of Populus trichocarpa Torr. & Gray.

    abstract::To explore what role chloroplast positioning might have in relation to latitudinal variation in mesophyll conductance (gm) of Populus trichocarpa Torr. & Gray (black cottonwood), we examined photosynthetic response to different blue light treatments in six representative genotypes (three northern and three southern). ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.03.006

    authors: Momayyezi M,Guy RD

    更新日期:2017-06-01 00:00:00

  • Seasonal variation of δ18O and δ2H in leaf water of Fagus sylvatica L. and related water compartments.

    abstract::The study aims to assess variability in leaf water isotopic enrichment occurring in the field under natural conditions. We focused on seasonal variation and difference between sun-exposed and shaded leaves. Isotopic composition (δ18O, δ2H) of leaf water was monitored in a beech tree (Fagus sylvatica L.) growing in the...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2018.03.009

    authors: Plavcová L,Hronková M,Šimková M,Květoň J,Vráblová M,Kubásek J,Šantrůček J

    更新日期:2018-08-01 00:00:00

  • Isolation of microprotoplasts from a partially synchronized suspension culture of Citrus unshiu.

    abstract::The effect of hydroxyurea (HU) and amiprophos-methyl (APM) on the synchronization of suspension cultures of Satsuma mandarin (Citrus unshiu) and micronucleation of the suspension cells sequentially treated with both, HU and APM, were investigated. When suspension cultures in early-log phase were treated with 4 or 10mM...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.09.003

    authors: Zhang Q,Liu J,Deng X

    更新日期:2006-11-01 00:00:00

  • Expression and variability of molecular chaperones in the sugarcane expressome.

    abstract::Molecular chaperones perform folding assistance in newly synthesized polypeptides preventing aggregation processes, recovering proteins from aggregates, among other important cellular functions. Thus their study presents great biotechnological importance. The present work discusses the mining for chaperone-related seq...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2006.03.013

    authors: Borges JC,Cagliari TC,Ramos CH

    更新日期:2007-04-01 00:00:00

  • TaEXPB7-B, a β-expansin gene involved in low-temperature stress and abscisic acid responses, promotes growth and cold resistance in Arabidopsis thaliana.

    abstract::Low temperature is one of the primary causes of economic loss in agricultural production, and in this regard, expansin proteins are known to play important roles in plant growth and responses to various abiotic stresses and plant hormones. In order to elucidate the roles of expansin genes in the response of Dongnongdo...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2019.153004

    authors: Feng X,Xu Y,Peng L,Yu X,Zhao Q,Feng S,Zhao Z,Li F,Hu B

    更新日期:2019-09-01 00:00:00

  • Characterization of starch phosphorylase from the marine green microalga (Chlorophyta) Tetraselmis subcordiformis reveals its potential role in starch biosynthesis.

    abstract::In a marine green starch-producing microalga Tetraselmis subcordiformis, the role of starch phosphorylase (SP) in the starch biosynthesis was disclosed by characterizing the enzyme properties and activity variations during the starch accumulation process. TsSP4, a SP isoform accounting for the major SP activity in T. ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.07.019

    authors: Jiang J,Yao C,Cao X,Liu Y,Xue S

    更新日期:2017-11-01 00:00:00

  • Bicarbonate in vivo Requirement of Photosystem II in the Green Alga Chlamydobotrys stellata.

    abstract::Flash induced 685 nm fluorescence emission of preilluminated and dark kept Chlamydobotrys stellata has been measured under conditions of CO(2)-deprivation. The difference in fluorescence intensity between dark kept and preilluminated cells is taken as a measure for the reduced state of the primary stable electron acce...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(85)80227-3

    authors: Mende D,Wiessner W

    更新日期:1985-03-01 00:00:00

  • Analysis of apoplastic and symplastic antioxidant system in shallot leaves: impacts of weak static electric and magnetic field.

    abstract::Impacts of electric and magnetic fields (EFs and MFs) on a biological organism vary depending on their application style, time, and intensities. High intensity MF and EF have destructive effects on plants. However, at low intensities, these phenomena are of special interest because of the complexity of plant responses...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2012.03.011

    authors: Cakmak T,Cakmak ZE,Dumlupinar R,Tekinay T

    更新日期:2012-07-15 00:00:00

  • Variations in the level of enzyme activity and immunolocalization of calcium-dependent protein kinases in the phloem of different cucumber organs.

    abstract::Calcium-dependent protein kinases (CDPKs) constitute a unique family of enzymes in plants that are characterized by a C-terminal calmodulin (CaM)-like domain. Through protein kinase assays, we have examined the levels of cucumber calcium-dependent kinase (CsCDPK) activity in various organs of cucumber seedlings and pl...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2003.11.010

    authors: Kumar KG,Jayabaskaran C

    更新日期:2004-08-01 00:00:00

  • Inhibition of stem elongation in spinach by theobroxide.

    abstract::In the current study, we investigated the influences of theobroxide on stem elongation in spinach (Spinacia oleracea). Our results showed that stem elongation and flower formation were inhibited by spraying spinach plants with theobroxide under inductive, long day conditions (16 h light/8 h dark), while application of...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.05.005

    authors: Kong F,Gao X,Nam KH,Takahashi K,Matsuura H,Yoshihara T

    更新日期:2006-03-01 00:00:00

  • Intrinsic stability of Brassicaceae plasma membrane in relation to changes in proteins and lipids as a response to salinity.

    abstract::Changes in plasma membrane lipids, such as sterols and fatty acids, have been observed as a result of salt stress. These alterations, together with modification of the plasma membrane protein profile, confer changes in the physical properties of the membrane to be taken into account for biotechnological uses. In our e...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2014.12.003

    authors: Chalbi N,Martínez-Ballesta MC,Youssef NB,Carvajal M

    更新日期:2015-03-01 00:00:00

  • Phylogenic and phosphorylation regulation difference of phosphoenolpyruvate carboxykinase of C3 and C4 plants.

    abstract::In C4 plants, phosphoenolpyruvate carboxykinase (PEPCK) plays a key role in the C4 cycle. PEPCK is also involved in gluconeogenesis and is conserved in both lower and higher organisms, including in animals and plants. A phylogenic tree constructed from PEPCK sequences from bacteria to higher plants indicates that the ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.02.008

    authors: Shen Z,Dong XM,Gao ZF,Chao Q,Wang BC

    更新日期:2017-06-01 00:00:00

  • Monodehydroascorbate reductase 2 and dehydroascorbate reductase 5 are crucial for a mutualistic interaction between Piriformospora indica and Arabidopsis.

    abstract::Ascorbate is a major antioxidant and radical scavenger in plants. Monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR) are two enzymes of the ascorbate-glutathione cycle that maintain ascorbate in its reduced state. MDAR2 (At3g09940) and DHAR5 (At1g19570) expression was upregulated in the roots ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2008.12.016

    authors: Vadassery J,Tripathi S,Prasad R,Varma A,Oelmüller R

    更新日期:2009-08-15 00:00:00

  • Half-life of tRNA and the Initial Rate of tRNA Accumulation in Plant Cells from Cell Suspension Cultures of Lupinus angustifolius cv. Turkus and Petroselinum hortense cv. Berlińska Growing at Different Rates.

    abstract::Half-lives of tRNA and the initial rates of tRNA accumulation are correlated with the growth rates of cell suspension cultures of Lupinus angustifolius cv. Turkus and Petroselinum hortense cv. Berlinska. Half-lives of tRNA are approximately proportional to the doubling times of cells in these cultures. The initial rat...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(84)80086-3

    authors: Sroga GE

    更新日期:1984-08-01 00:00:00

  • Nitrogen-source preference in blueberry (Vaccinium sp.): Enhanced shoot nitrogen assimilation in response to direct supply of nitrate.

    abstract::Blueberry (Vaccinium sp.) is thought to display a preference for the ammonium (NH4+) form over the nitrate (NO3-) form of inorganic nitrogen (N). This N-source preference has been associated with a generally low capacity to assimilate the NO3- form of N, especially within the shoot tissues. Nitrate assimilation is med...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.05.014

    authors: Alt DS,Doyle JW,Malladi A

    更新日期:2017-09-01 00:00:00

  • Responses of hybrid aspen over-expressing a PIP2;5 aquaporin to low root temperature.

    abstract::Aquaporins mediate the movement of water across cell membranes. Plasma membrane intrinsic protein 2;5 from Populus trichocarpa×deltoides (PtdPIP2;5) was previously demonstrated to be a functionally important water conducting aquaporin. To study the relevance of aquaporin-mediated root water transport at low temperatur...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2016.02.001

    authors: Ranganathan K,El Kayal W,Cooke JE,Zwiazek JJ

    更新日期:2016-03-15 00:00:00

  • Plasticity in stomatal size and density of potato leaves under different irrigation and phosphorus regimes.

    abstract::The morphological features of stomata including their size and density could be modulated by environmental cues; however, the underlying mechanisms remain largely elusive. Here, the effect of different irrigation and phosphorus (P) regimes on stomatal size (SS) and stomatal density (SD) of potato leaves was investigat...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2014.06.002

    authors: Sun Y,Yan F,Cui X,Liu F

    更新日期:2014-09-01 00:00:00

  • Characterization the role of a UFC homolog, AtAuxRP3, in the regulation of Arabidopsis seedling growth and stress response.

    abstract::Auxin is a well-known, crucial regulator of the entire plant lifecycle, not only orchestrating many aspects of plant growth and development, but also playing various roles in biotic and abiotic stress. This study reports the isolation and functional characterization of a DUF-966 domain-containing gene, At3g46110, re-n...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2019.152990

    authors: Shen L,Zhong T,Wang L,Zhang Q,Jin H,Xu M,Ye J

    更新日期:2019-09-01 00:00:00

  • Molecular cloning and characterization of a cDNA encoding a ryegrass (Lolium perenne) ENOD40 homologue.

    abstract::The ENOD40 gene, found in several leguminous and non-leguminous plant species is expressed in the pericycle of legume roots early in the nodulation process, adjacent to the protoxylem poles, but before the cortical cells divide to form the nodule itself. The ENOD40 transcript contains only short open reading frames wh...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1078/0176-1617-00962

    authors: Larsen K

    更新日期:2003-06-01 00:00:00

  • Effect of salinity on root-nodule conductance to the oxygen diffusion in the Cicer arietinum-Mesorhizobium ciceri symbiosis.

    abstract::Nodule conductance to O2 diffusion has been involved as a major factor of the inhibition of N2 fixation by soil salinity that severely reduces the production of grain legumes. In order to determine the effect of this constraint on the nodule conductance, oxygen uptake by the nodulated roots of Cicer arietinum was meas...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2006.05.016

    authors: L'taief B,Sifi B,Zaman-Allah M,Drevon JJ,Lachaâl M

    更新日期:2007-08-01 00:00:00

  • Abscisic Acid-related changes in composition and physical state of membranes in bean leaves.

    abstract::Leaf discs of bean, maize, and wheat were incubated in the presence of either 2-(14)C mevalonic acid or methyl-(14)C choline chloride and varying concentrations of abscisic acid. Abscisic acid inhibited, dependent on concentration, the incorporation of mevalonate into free sterols and stimulated the incorporation of c...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(85)80197-8

    authors: Farkas T,Singh B,Nemecz G

    更新日期:1985-03-01 00:00:00

  • Separation of abscission zone cells in detached Azolla roots depends on apoplastic pH.

    abstract::In studies on the mechanism of cell separation during abscission, little attention has been paid to the apoplastic environment. We found that the apoplastic pH surrounding abscission zone cells in detached roots of the water fern Azolla plays a major role in cell separation. Abscission zone cells of detached Azolla ro...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2012.08.008

    authors: Fukuda K,Yamada Y,Miyamoto K,Ueda J,Uheda E

    更新日期:2013-01-01 00:00:00

  • Overexpression of Crocus carotenoid cleavage dioxygenase, CsCCD4b, in Arabidopsis imparts tolerance to dehydration, salt and oxidative stresses by modulating ROS machinery.

    abstract::Apocarotenoids modulate vital physiological and developmental processes in plants. These molecules are formed by the cleavage of carotenoids, a reaction catalyzed by a family of enzymes called carotenoid cleavage dioxygenases (CCDs). Apocarotenoids like β-ionone and β-cyclocitral have been reported to act as stress si...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2015.11.001

    authors: Baba SA,Jain D,Abbas N,Ashraf N

    更新日期:2015-09-15 00:00:00

  • Clover and ryegrass are tolerant species to ammonium nutrition.

    abstract::The application of nitrification inhibitors (NIs) together with nitrogen fertilizers in grasslands is an effective alternative to reduce nitrate leaching and nitrogenous gases emissions to the atmosphere. Nevertheless, the use of NIs increases the amount of ammonium available for the plant that, due to its reported to...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2006.11.013

    authors: Belastegui-Macadam XM,Estavillo JM,García-Mina JM,González A,Bastias E,Gónzalez-Murua C

    更新日期:2007-12-01 00:00:00

  • Similarities and differences in the properties of multiple NDP-kinase isoforms of Alyssum murale, Ni2+-accumulator species.

    abstract::Two isoforms of NDPKs (diphosphonucleoside kinases: E.C. 2.7.4.6.) named S-NDPK-A and S-NDPK-B were separated and purified from shoots of Alyssum murale (19th day of growth), a nickel accumulator plant, by a four-step procedure involving ammonium sulphate precipitation and DEAE-sepharose and hydroxyapatite column chro...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2010.01.016

    authors: Siskos A,Yupsani A,Symeonidis L,Yupsanis T

    更新日期:2010-06-15 00:00:00

  • Nitrogen metabolism in leaves of a tank epiphytic bromeliad: characterization of a spatial and functional division.

    abstract::The leaf is considered the most important vegetative organ of tank epiphytic bromeliads due to its ability to absorb and assimilate nutrients. However, little is known about the physiological characteristics of nutrient uptake and assimilation. In order to better understand the mechanisms utilized by some tank epiphyt...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2011.01.008

    authors: Takahashi CA,Mercier H

    更新日期:2011-07-15 00:00:00

  • The spaceflight environment can induce transpositional activation of multiple endogenous transposable elements in a genotype-dependent manner in rice.

    abstract::Spaceflight represents a unique environmental condition whereby dysregulated gene expression and genomic instability can be provoked. However, detailed molecular characterization of the nature of genetic changes induced by spaceflight is yet to be documented in a higher eukaryote. Transposable elements (TEs) are ubiqu...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2009.06.007

    authors: Long L,Ou X,Liu J,Lin X,Sheng L,Liu B

    更新日期:2009-12-15 00:00:00

  • Multivariable environmental conditions promote photosynthetic adaptation potential in Arabidopsis thaliana.

    abstract::Most of our knowledge on the regulation of photosynthesis originates from studies performed in highly controlled laboratory conditions. However, in their natural habitats plants are simultaneously subjected to a broad range of abiotic and biotic stimuli which influence photosynthetic efficiency; hence there is an emer...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2012.11.016

    authors: Wituszyńska W,Gałązka K,Rusaczonek A,Vanderauwera S,Van Breusegem F,Karpiński S

    更新日期:2013-04-15 00:00:00