Abstract:
:The uptake of liposomal contents into Catharanthus roseus protoplasts has been quantitated. Hi.phest uptake was obtained from positively charged vesicles (1.09% of vesicle contents per 10(7) protoplasts) in the presence of 10% w/v polyethylene glycol 4000. Significant uptake was also observed from negatively charged vesicles (0.1% of vesicle contents per 10(7) protoplasts) in the presence of 25% polyethylene glycol 1540. The uptake of vesicle contents from negatively charged liposomes was confirmed by light microscopy after treatment of the protoplasts with liposomes loaded with the fluorescent dye 6-carboxyfluorescein. About 10% of the treated protoplasts exhibited intracellular fluorescence. No uptake from neutral liposomes was detected.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Cutler AJ,Shargool PD,Kurz WG,Constabel Fdoi
10.1016/S0176-1617(84)80014-0subject
Has Abstractpub_date
1984-11-01 00:00:00pages
29-40issue
1eissn
0176-1617issn
1618-1328pii
S0176-1617(84)80014-0journal_volume
117pub_type
杂志文章abstract::We investigated the response of extracellular phosphatase to heat shock in heterotrophic Chenopodium rubrum L. cell cultures. Surprisingly, in contrast to the generally used acid phosphatase, an extracellular alkaline phosphatase showed the most sensitive response. This phosphatase was characterized as a marker for ce...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.12.012
更新日期:2008-11-01 00:00:00
abstract::Rice is the staple food for more than fifty percent of the world's population, and is therefore an important crop. However, its production is hindered by several biotic and abiotic stresses. Although rice is the only crop that can germinate even in the complete absence of oxygen (i.e. anoxia), flooding (low oxygen) is...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.07.009
更新日期:2011-12-15 00:00:00
abstract::Fruit trees are likely to suffer from the effects of severe drought in the future; however, sound criteria for evaluating the species' ability to survive these extreme conditions are largely missing. Here, we evaluated the feasibility of using xylem cavitation resistance as a tool for screening Prunus species for drou...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.07.020
更新日期:2008-06-16 00:00:00
abstract::Seedlings of Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and Alemow (Citrus macrophylla Wester) were inoculated with a mixture of AM fungi (Rhizophagus irregularis and Funneliformis mosseae) (+AM), or left non-inoculated (-AM). From forty-five days after fungal inoculation onwards, half of +AM or -AM plants were ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.06.006
更新日期:2014-01-01 00:00:00
abstract::Arbuscular mycorrhizal fungi enhance CO₂ assimilation of their hosts which ensure the demand for carbohydrates of these obligate biotrophic microorganisms. Photosynthetic parameters were measured in tomato colonised or not by the arbuscular mycorrhizal fungus Glomus mosseae. In addition, carbohydrate contents and mRNA...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.01.026
更新日期:2011-07-15 00:00:00
abstract::Plant antioxidants ascorbate and glutathione play an important role in regulating potentially harmful reactive oxygen species produced in response to virus infection. Barley yellow dwarf virus is a widespread viral pathogen that systemically infects cereal crops including wheat, barley and oats. In addition, rising at...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.05.007
更新日期:2016-07-20 00:00:00
abstract::Using glass microelectrodes we studied (in spinach, a long-day plant) the characteristics of the intracellular potential of mesophyll cells as well as its response towards light, in particular during the transfer of the plant to an inductive photoperiod (transfer from short day to continuous photoperiod). When 20 minu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(85)80207-8
更新日期:1985-04-01 00:00:00
abstract::Plant tissue growth can be regulated and controlled via culture media composition. A number of different laborious and time-consuming approaches have been used to attempt development of optimized media for a wide range of species and applications. However, plant tissue culture is a very complex task, and the identific...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.04.008
更新日期:2011-10-15 00:00:00
abstract::Impacts of electric and magnetic fields (EFs and MFs) on a biological organism vary depending on their application style, time, and intensities. High intensity MF and EF have destructive effects on plants. However, at low intensities, these phenomena are of special interest because of the complexity of plant responses...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.03.011
更新日期:2012-07-15 00:00:00
abstract::Hyacinth (Hyacinthus orientalis) bulbs infected by Fusarium oxysporum showed the symptoms of gummosis. The purpose of this study was to clarify the hormonal regulation of gummosis and composition of gums from hyacinth bulbs. The application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2014.10.007
更新日期:2015-02-01 00:00:00
abstract::Sun (Chrysanthemum morifolium) and shade (Spathiphyllum wallisii) plants were used to study the effects of drought, heat and high illumination. The stress conditions caused a greater accumulation of hydrogen peroxide in Chrysanthemum morifolium than in Spathiphyllum wallisii leaves. They also resulted in down-regulati...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.013
更新日期:2010-06-15 00:00:00
abstract::ALA is a key precursor in the biosynthesis of porphyrins such as chlorophyll and heme, and was found to induce temporary elevations in the photosynthesis rate, APX, and CAT; furthermore, treatment with ALA at a low concentration might be correlated to the increase of NaCl tolerance of spinach plants. The photosyntheti...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-00991
更新日期:2003-09-01 00:00:00
abstract::Protein metabolism and expression play important role in plant adaptation to water stress. The objectives of this study were to examine proteomic responses to water stress induced by polyethylene glycol (PEG) in creeping bentgrass (Agrostis stolonifera L.) leaves and to identify proteins associated with stress toleran...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.05.006
更新日期:2010-11-15 00:00:00
abstract::It has been shown that oxidative damage contributes to the wide range of toxic effects of the mycotoxin ochratoxin A (OTA). Therefore, we examined the effects of alpha-tocopherol (alpha-TOC) and different polyphenols--catechin (CAT), daidzein (DAI), epicatechin (EC), epigallocatechin gallate (EGCG), genistein (GEN), a...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.04.021
更新日期:2005-07-01 00:00:00
abstract::We present here the comparative protective potentiality of exogenously applied polyamines (PAs), namely spermidine (Spd) and spermine (Spm), in mitigating NaCl toxicity and inducing short-term salinity tolerance in three indica rice varieties, namely M-1-48 (salt-sensitive), Nonabokra (salt-tolerant) and Gobindobhog (...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.07.009
更新日期:2011-03-01 00:00:00
abstract::The purification of 6-O(4-O)-indole-3-ylacetyl-beta-D-glucose (IAGlc) hydrolase from immature kernels of maize (Zea mays) was undertaken to separate this enzyme from 1-O-IAGlc hydrolase and beta-glucosidase. Partially purified 6-O(4-O)-IAGlc hydrolase was found to be the specific enzyme catalyzing hydrolysis of stable...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.05.015
更新日期:2005-02-01 00:00:00
abstract::Some intracellular organelles found in eukaryotes such as plants have arisen through the endocytotic engulfment of prokaryotic cells. This accounts for the presence of plant membrane intrinsic proteins that have homologs in prokaryotic cells. Other organelles, such as those of the endomembrane system, are thought to h...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.09.022
更新日期:2014-05-15 00:00:00
abstract::Raffinose synthase (RS, EC2.4.1.82) is one of the key enzymes that channels sucrose into the raffinose family oligosaccharides (RFOs) biosynthetic pathway. However, the gene encoding RS is poorly characterized in cucumber (Cucumis sativus L.), which is a typical RFOs-translocating plant species. Here we isolated the g...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.019
更新日期:2012-12-15 00:00:00
abstract::Cell wall polysaccharides, pectin composition, as well as apoplastic superoxide dismutase and peroxidase activities were investigated in strawberry (Fragaria x ananassa) cultivars (cvs) Korona and Elsanta differing in their ozone sensitivity. Plants were exposed to 140-170 microg m(-3) ozone either short-term for 7 da...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.06.012
更新日期:2008-05-26 00:00:00
abstract::Eight cultivars of rice (Oryza sativa L.) inhibited shoot and root growth of Echinochloa crus-galli when co-cultured with rice seedlings in a bioassay medium. Momilactone A and B were found in the bioassay medium of all rice cultivars, and concentrations of momilactone A and B in the medium were 0.21-1.5 and 0.66-3.8 ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.01.014
更新日期:2010-07-01 00:00:00
abstract::The microinjection of fluorescent probes into live cells is an essential component in the toolbox of modern cell biology. Microinjection techniques include the penetration of the plasma membrane and, if present, the cell wall with micropipettes, and the application of pressure or electrical currents to drive the micro...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153060
更新日期:2020-01-01 00:00:00
abstract::Replacement of N(2) by argon in the air around nodules directs nitrogenase electron flow in its total onto H(+) resulting in increased nodule H(2) evolution (total nitrogenase activity (TNA)). However, argon application induces a so-called argon-induced decline in nitrogenase activity (Ar-ID) connected with decreased ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.03.014
更新日期:2010-09-01 00:00:00
abstract::A non-destructive nitrogen (N) detector [Agriexpert PPW-3000 (PPW-3000)] is a useful device for rapid and non-destructive measurement of leaf N content. However, some studies find a poor correlation between the PPW-3000 reading and the actual leaf N content; the R2 value of the approximate equation was low. To improve...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.09.004
更新日期:2006-04-01 00:00:00
abstract::Estimates of mesophyll conductance (gm), when calculated from chlorophyll fluorescence, are uncertain, especially when the photosystem II (PSII) operating efficiency is measured from the traditional single saturation pulse methodology. The multiphase flash method has recently been recommended to replace the single sat...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.11.009
更新日期:2018-01-01 00:00:00
abstract::Nod factors (Lipo-chitooligosaccharides, or LCOs) act as bacteria-to-plant signal molecules that modulate early events of the Bradyrhizobium-soybean symbiosis. It is known that low root zone temperature inhibits the early stages of this symbiosis; however, the effect of low soil temperature on bacteria-to-plant signal...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.04.029
更新日期:2006-01-01 00:00:00
abstract::Cell suspension cultures of Silybum marianum are able to excrete silymarin compounds into the medium upon elicitation with methyl jasmonate or cyclodextrins. Knowledge of transport mechanism is important to understand Sm metabolism and to develop strategies aimed at increasing production by means of cell cultures. For...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.10.005
更新日期:2014-01-15 00:00:00
abstract::The efficacy of calcium sprays for improving fleshy fruit resistance to abiotic/biotic stress and enhancement of fruit shelf life has increasingly been explored. However, because calcium is a powerful secondary messenger in many signaling pathways, including those driven by abscisic acid (ABA) and jasmonates, it may i...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.08.011
更新日期:2018-12-01 00:00:00
abstract::The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-spe...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.04.018
更新日期:2005-11-01 00:00:00
abstract::Ammonium is a paradoxical nutrient ion. Despite being a common intermediate in plant metabolism whose oxidation state eliminates the need for its reduction in the plant cell, as occurs with nitrate, it can also result in toxicity symptoms. Several authors have reported that carbon enrichment in the root zone enhances ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.12.015
更新日期:2013-05-15 00:00:00
abstract::Common sage (Salvia officinalis L., Lamiaceae) is one of the most important medicinal and aromatic plants, with antioxidant, antimicrobial, spasmolytic, astringent, antihidrotic and specific sensorial properties. The essential oil of the plant, composed mainly of the monoterpenes 1,8-cineole, alpha-thujone, beta-thujo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.009
更新日期:2010-07-01 00:00:00