Abstract:
:Protein metabolism and expression play important role in plant adaptation to water stress. The objectives of this study were to examine proteomic responses to water stress induced by polyethylene glycol (PEG) in creeping bentgrass (Agrostis stolonifera L.) leaves and to identify proteins associated with stress tolerance. Plants of two cultivars ('Penncross' and 'Penn-A4') differing in water stress tolerance were grown in sand irrigated daily with water (control) or PEG solution (osmotic potential of -0.66MPa) to induce water stress, for 28d in growth chambers. Shoot extension rate, relative water content and cell membrane stability were measured to compare drought tolerance between the two cultivars. All parameters maintained at a significantly higher level in 'Penn-A4' than in 'Penncross' under PEG treatment. After 28d of water stress, proteins were extracted from leaves and separated by difference gel electrophoresis. Among 56 stress-responsive protein spots, 46 were identified using mass spectrometry. Some proteins involved in primary nitrogen and carbon metabolism were down-regulated by PEG-induced water stress in both cultivars. The abundance of antioxidant enzyme proteins (ascorbate peroxidase, catalase and glutathione-S-transferase) increased under water stress, particularly ascorbate peroxidase in 'Penn-A4'. The abundance levels of actins, UDP-sulfoquinovose synthase and glucan exohydrolase were greater in 'Penn-A4' than in 'Penncross' under PEG treatment. Our results suggest that proteins involved in membrane synthesis, cell wall loosening, cell turgor maintenance, and antioxidant defense may play roles in perennial grass adaptation to PEG-induced water stress.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Xu C,Huang Bdoi
10.1016/j.jplph.2010.05.006subject
Has Abstractpub_date
2010-11-15 00:00:00pages
1477-85issue
17eissn
0176-1617issn
1618-1328pii
S0176-1617(10)00231-2journal_volume
167pub_type
杂志文章abstract::Aquaporins mediate the movement of water across cell membranes. Plasma membrane intrinsic protein 2;5 from Populus trichocarpa×deltoides (PtdPIP2;5) was previously demonstrated to be a functionally important water conducting aquaporin. To study the relevance of aquaporin-mediated root water transport at low temperatur...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.02.001
更新日期:2016-03-15 00:00:00
abstract::Using glass microelectrodes we studied (in spinach, a long-day plant) the characteristics of the intracellular potential of mesophyll cells as well as its response towards light, in particular during the transfer of the plant to an inductive photoperiod (transfer from short day to continuous photoperiod). When 20 minu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(85)80207-8
更新日期:1985-04-01 00:00:00
abstract::Tomato (Solanum lycopersicum L.) plants were transformed with an antisense construct of a cDNA encoding tomato telomere-binding protein (LeTBP1) to describe the role of a telomere-binding protein at the whole plant level. Fruit size decreased corresponding to the degree of suppression of LeTBP1 expression. This inhibi...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.05.009
更新日期:2011-11-01 00:00:00
abstract::Drought stress is considered the most adverse factor restricting plant survival, growth, and productivity. The identification of the key adaptive mechanisms to drought stress is essential to enhance the drought resistance of plants. In this study, differential responses of three alfalfa varieties to drought, including...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.10.023
更新日期:2019-01-01 00:00:00
abstract::The present study was undertaken to test the influence of exogenously applied 10nM brassinolide (BL) on the abscisic acid (ABA) content in Chlorella vulgaris cultures subjected to short-term (3h) heat stress (30-40 degrees C). We found that BL enhanced the ABA content with increase in temperature. In parallel, under t...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.10.004
更新日期:2009-05-15 00:00:00
abstract::A sand culture experiment was conducted to answer the question whether or not exogenous KNO(3) can alleviate adverse effects of salt stress in winter wheat by monitoring plant growth, K(+)/Na(+) accumulation and the activity of some antioxidant enzymes. Seeds of two wheat cultivars (CVs), DK961 (salt-tolerant) and JN1...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.01.001
更新日期:2008-09-29 00:00:00
abstract::Acute ozone is a model abiotic elicitor of oxidative stress in plants. In order to identify genes that are important for conferring ozone resistance or sensitivity we used two accessions of Medicago truncatula with contrasting responses to this oxidant. We used suppression subtraction hybridization (SSH) to identify g...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.01.009
更新日期:2009-08-15 00:00:00
abstract::Seed germination is sensitive to glucose (Glc), nitric oxide (NO) and polyamine (PA). To elucidate whether cross-talk among Glc, NO and PAs occurs in mediation of seed germination, effects of Glc, NO and spermine on seed germination of Lotus japonicus were studied. Glc retarded seed germination in a concentration-depe...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.03.001
更新日期:2009-01-30 00:00:00
abstract::The toxicity and effects on protein synthesis of the phthalate esters diethyl phthalate (DEP) and di(2-ethylhexyl) phthalate (DEHP) was studied in radish seedings (Raphanus sativus cv. Kööpenhaminan tori). Phthalate esters are a class of commercially important compounds used mainly as plasticizers in high molecular-we...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-00525
更新日期:2003-09-01 00:00:00
abstract::Previous studies have shown that plants developed under high relative air humidity (RH>85%) develop malfunctioning stomata and therefor have increased transpiration and reduced desiccation tolerance when transferred to lower RH conditions and darkness. In this study, plants developed at high RH were exposed to daily V...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.12.011
更新日期:2017-04-01 00:00:00
abstract::We report in this study, the successful deployment of a double mutant acetolactate synthase gene (ALSdm, containing Pro 197 to Ser and Ser 653 to Asn substitutions) as an efficient in vitro selection marker for the development of transgenic plants in Brassica juncea (oilseed mustard). The ALS enzyme is inhibited by tw...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.02.001
更新日期:2004-09-01 00:00:00
abstract::Sweet potato (Ipomoea batatas L.) is an important crop in the world, cultivated in temperate climates under low inputs. Drought changes the plant biomass allocation, together with the carbon and nitrogen isotopic composition (δ13C and δ15N), whose changes are faintly known in sweet potato crops. Here, we show the biom...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153052
更新日期:2019-12-01 00:00:00
abstract::The predicted accentuation of drought events highlights the importance of optimize plants capacity to tolerate drought, but also the capacity to recovery from it, especially in species, as olive tree (Olea europaea L.), that grows in particularly susceptible regions. Three different concentrations (10, 100 and 1000 μM...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.08.004
更新日期:2018-11-01 00:00:00
abstract::Germination is a complex process, highly dependent on various environmental factors, including temperature and water availability. Germinating soybean seeds are especially vulnerable to unfavorable environmental conditions and exposure to long-term abiotic stresses may result in diminishing much of the yield and most ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.11.020
更新日期:2013-03-15 00:00:00
abstract::The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, s...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.06.017
更新日期:2010-01-01 00:00:00
abstract::Nilaparvata lugens Stål, the brown planthopper (BPH), is one of the most destructive phloem-feeding insects of rice (Oryza sativa L.) throughout Asia. Here, we show that BPH feeding increases the level of endogenous nitric oxide (NO) in the leaf and sheath tissue of both resistant and susceptible rice cultivars. Howev...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.09.018
更新日期:2011-05-15 00:00:00
abstract::The epiphytic fern Pleopeltis polypodioides can tolerate repeated drying and rehydration events without conspicuous damage. To understand the biochemical principles of drought-tolerance, we analyzed the effect of dehydration and rehydration at 25 °C on hydroperoxide and lipid hydroperoxide, the activities of antioxida...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.05.006
更新日期:2018-09-01 00:00:00
abstract::Auxin is a well-known, crucial regulator of the entire plant lifecycle, not only orchestrating many aspects of plant growth and development, but also playing various roles in biotic and abiotic stress. This study reports the isolation and functional characterization of a DUF-966 domain-containing gene, At3g46110, re-n...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.152990
更新日期:2019-09-01 00:00:00
abstract::Sun (Chrysanthemum morifolium) and shade (Spathiphyllum wallisii) plants were used to study the effects of drought, heat and high illumination. The stress conditions caused a greater accumulation of hydrogen peroxide in Chrysanthemum morifolium than in Spathiphyllum wallisii leaves. They also resulted in down-regulati...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.013
更新日期:2010-06-15 00:00:00
abstract::A critical stage in pollen development is the dissolution of tetrads into free microspores. Tetrads are surrounded by a wall composed primarily of beta-1,3-glucan. At the completion of meiosis, tetrads are released into the anther locule after hydrolysis of the callose by a beta-1,3-glucanase complex. The cDNA corresp...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01207
更新日期:2003-11-01 00:00:00
abstract::The leaf is considered the most important vegetative organ of tank epiphytic bromeliads due to its ability to absorb and assimilate nutrients. However, little is known about the physiological characteristics of nutrient uptake and assimilation. In order to better understand the mechanisms utilized by some tank epiphyt...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.01.008
更新日期:2011-07-15 00:00:00
abstract::Raffinose synthase (RS, EC2.4.1.82) is one of the key enzymes that channels sucrose into the raffinose family oligosaccharides (RFOs) biosynthetic pathway. However, the gene encoding RS is poorly characterized in cucumber (Cucumis sativus L.), which is a typical RFOs-translocating plant species. Here we isolated the g...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.019
更新日期:2012-12-15 00:00:00
abstract::The expression of foreign phytoalexins in a new host is thought to increase fungal resistance, since host-specific pathogens have not experienced selection for detoxifying or metabolising the novel antifungal compounds. Two resveratrol synthase genes vst1 and vst2 from grapevine (Vitis vinifera L.) and the pinosylvin ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.11.005
更新日期:2005-09-01 00:00:00
abstract::Calcium oxalate crystals are a major biomineralization product in higher plants. Their biological function and use are not well understood. In this work, we focus on the isolation and crystallochemical characterization of calcium oxalate crystals from seed coats of Phaseolus vulgaris (prisms) and leaves of Vitis vinif...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-00947
更新日期:2003-03-01 00:00:00
abstract::The effect of excess copper on the expression of soluble proteins in 10-day old Phaseolus vulgaris seedlings was studied with two-dimensional electrophoresis and mass spectrometry, to find sensitive biochemical markers of exposure. Despite major differences in root Cu contents, both 15 and 50 microM Cu treatments resu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.07.018
更新日期:2005-04-01 00:00:00
abstract::Rhizoctonia solani is a fungal pathogen that causes sheath blight disease in rice plants. In this study, metabolomic analysis using CE/TOF-MS in negative ion mode was used to investigate the resistance response of resistant and susceptible rice lines (32R and 29S, respectively) due to R. solani infection. Two rice lin...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.09.004
更新日期:2016-11-01 00:00:00
abstract::The miR172 gene is involved in the regulation of flowering time and floral organ identity in Arabidopsis thaliana through regulation of APETALA2 (AP2)-like genes' activity. AP2 plays critical roles in establishing meristem and organ identity during floral development. Additionally, the AP2-like genes including TARGET ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.05.011
更新日期:2009-11-01 00:00:00
abstract::Leaf discs of bean, maize, and wheat were incubated in the presence of either 2-(14)C mevalonic acid or methyl-(14)C choline chloride and varying concentrations of abscisic acid. Abscisic acid inhibited, dependent on concentration, the incorporation of mevalonate into free sterols and stimulated the incorporation of c...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(85)80197-8
更新日期:1985-03-01 00:00:00
abstract::Suppression of photorespiration by low O2 concentrations (Method 1) and simultaneous measurements of gas exchange and chlorophyll fluorescence (Method 2) are often used to estimate leaf photorespiration rate (Rp) of C3 plants. However, it is largely unknown whether Method 1 and Method 2 can be used equivalently in est...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153002
更新日期:2019-09-01 00:00:00
abstract::In open-field soilless culture there can be great deal of leaching, particularly in rainy springs. Ligneous plants have the capacity to store large quantities of nutrients in perennial organs. Knowledge of the plant's internal nutrient mobilisation during spring to supply growing organs could lead to reduction of fert...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.03.012
更新日期:2007-05-01 00:00:00