Abstract:
:Acute ozone is a model abiotic elicitor of oxidative stress in plants. In order to identify genes that are important for conferring ozone resistance or sensitivity we used two accessions of Medicago truncatula with contrasting responses to this oxidant. We used suppression subtraction hybridization (SSH) to identify genes differentially expressed in ozone-sensitive Jemalong and ozone-resistant JE154 following exposure to 300 nLL(-1) of ozone for 6h. Following differential screening of more than 2500 clones from four subtraction libraries, more than 800 clones were selected for sequencing. Sequence analysis of these clones identified 239 unique contigs. Fifteen novel genes of unknown functions were identified. A majority of the ozone responsive genes identified in this study were present in the Medicago truncatula EST collections. Genes induced in JE154 were associated with adaptive responses to stress, while in Jemalong, the gene ontologies for oxidative stress, cell growth, and translation were enriched. A meta-analysis of ozone responsive genes using the Genvestigator program indicated enrichment of ABA and auxin responsive genes in JE154, while cytokinin response genes were induced in Jemalong. In resistant JE154, down regulation of photosynthesis-related genes and up regulation of genes responding to low nitrate leads us to speculate that lowering carbon-nitrogen balance may be an important resource allocation strategy for overcoming oxidative stress. Temporal profiles of select genes using real-time PCR analysis showed that most of the genes in Jemalong were induced at the later time points and is consistent with our earlier microarray studies. Inability to mount an early active transcriptional reprogramming in Jemalong may be the cause for an inefficient defense response that in turn leads to severe oxidative stress and culminates in cell death.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Puckette M,Peal L,Steele J,Tang Y,Mahalingam Rdoi
10.1016/j.jplph.2009.01.009subject
Has Abstractpub_date
2009-08-15 00:00:00pages
1284-1295issue
12eissn
0176-1617issn
1618-1328pii
S0176-1617(09)00048-0journal_volume
166pub_type
杂志文章abstract::Recent data indicate the existence of at least three L-ascorbic acid (AsA) biosynthetic pathways in plant cells. Studying their occurrence in different plant organs and species may help to decipher the precise role(s) of AsA in plant cell physiology. In grape berries, AsA is of particular importance since it is known ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.12.017
更新日期:2010-06-15 00:00:00
abstract::Ammonium is a paradoxical nutrient ion. Despite being a common intermediate in plant metabolism whose oxidation state eliminates the need for its reduction in the plant cell, as occurs with nitrate, it can also result in toxicity symptoms. Several authors have reported that carbon enrichment in the root zone enhances ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.12.015
更新日期:2013-05-15 00:00:00
abstract::The application of urease inhibitors in conjunction with urea fertilizers as a means of reducing N loss due to ammonia volatilization requires an in-depth study of the physiological effects of these inhibitors on plants. The aim of this study was to determine how the urease inhibitor N-(n-butyl) thiophosphoric triamid...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.07.024
更新日期:2011-03-01 00:00:00
abstract::Drought is one of the stresses that limit the yield of barley. Despite extensive studies focused on the issue, the molecular mechanism of the response to drought is still not fully understood. In our previous study, we proposed drought-induced signal perception controlled by actin filaments (AFs). To test this hypothe...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.02.006
更新日期:2016-04-01 00:00:00
abstract::Syringyl lignins result from the oxidative polymerization of sinapyl alcohol in a reaction mediated by syringyl (basic) peroxidases. Several peroxidases have been identified in the genome of Arabidopsis thaliana as close homologues to ZePrx, the best characterized basic peroxidase so far, but none of these has been di...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2014.11.006
更新日期:2015-03-01 00:00:00
abstract::The Normalized Difference Vegetation Index (NDVI) is widely used for monitoring, analyzing, and mapping temporal and spatial distributions of physiological and biophysical characteristics of vegetation. It is well documented that the NDVI approaches saturation asymptotically under conditions of moderate-to-high aboveg...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01176
更新日期:2004-02-01 00:00:00
abstract::Isoprene (2-methyl-1,3-butadiene) is a hemiterpene molecule. It has been estimated that the plant kingdom emits 500-750 million tons of isoprene in the environment, half of which results from tropical broadleaf trees and the remainder from shrubs. Camelina (Camelina sativa (L.) Crantz) is an emerging bioenergy plant f...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.06.005
更新日期:2017-08-01 00:00:00
abstract::Calcineurin B-like (CBL) interacting protein kinase 15 (CIPK15) is a newly identified positive regulator which is critical to directing the O(2) deficiency signal to the sugar signaling cascade as part of Amy3D (representative Amy3 gene) regulation in rice. It is located upstream and probably contributes to reserve mo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.06.003
更新日期:2012-10-15 00:00:00
abstract::Increasing atmospheric CO₂ concentrations are expected to enhance plant photosynthesis and yield. Nevertheless, after long-term exposure, plants acclimate and show a reduction in photosynthetic activity (called down-regulation), which may cause a reduction in potential yield. Some authors suggest that down-regulation ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.06.015
更新日期:2010-12-15 00:00:00
abstract::The effect of five topolins (meta-Topolin=mT; meta-Topolin riboside=mTR; meta-Methoxy topolin=MemT; meta-Methoxy topolin riboside=MemTR and 6-(meta-methoxy)-9-(tetrahydropyran-2-yl)-topolin=MemTTHP) on the photosynthetic pigments and leaf structures of micropropagated 'Williams' bananas was compared with the commonly ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.06.006
更新日期:2012-10-15 00:00:00
abstract::Fruit trees are likely to suffer from the effects of severe drought in the future; however, sound criteria for evaluating the species' ability to survive these extreme conditions are largely missing. Here, we evaluated the feasibility of using xylem cavitation resistance as a tool for screening Prunus species for drou...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.07.020
更新日期:2008-06-16 00:00:00
abstract::Nitric oxide (NO) is a highly reactive, membrane-permeable free radical, which has recently emerged as an important antioxidant. Here we investigated the protective effect of NO against the toxicity and NH4+ accumulation in rice leaves caused by excess CuSO4 (10mmol L(-1)). It was found that free radical scavengers (s...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.02.003
更新日期:2005-12-01 00:00:00
abstract::In Lilium longiflorum, vernalization is both an obligatory requirement and the major factor affecting flowering time, however, little is known about the molecular regulation of this mechanism in Lilium and other flowering bulbs. Exposure of L. longiflorum bulbs to 9 weeks at 4°C greatly promoted stem elongation within...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2014.09.015
更新日期:2015-01-15 00:00:00
abstract::Previous results have shown that hydrogen sulfide (H2S), mainly catalyzed by l-cysteine desulfhydrase (DES) in plants, triggers adventitious rooting. The objective of this study was to test whether H2S is involved in methane (CH4)-induced adventitious root development in cucumber explants. First, we observed that the ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.05.010
更新日期:2018-09-01 00:00:00
abstract::The vegetative propagation in vitro of Gardenia jasminoides Ellis, an ornamental shrub producing secondary metabolites useful in perfumery and pharmacy, has been investigated. In the presence of benzylaminopurine (0.3 to 1 mg l(-1)) and indoleacetic acid (1 mg l(-1)), axillary shoots arose from nodes of greenhause pla...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80131-5
更新日期:1984-11-01 00:00:00
abstract::Plant antioxidants ascorbate and glutathione play an important role in regulating potentially harmful reactive oxygen species produced in response to virus infection. Barley yellow dwarf virus is a widespread viral pathogen that systemically infects cereal crops including wheat, barley and oats. In addition, rising at...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.05.007
更新日期:2016-07-20 00:00:00
abstract::The SaCLCa1 gene, a putative orthologue of AtCLCa, the Arabidopsis thaliana gene encoding a NO3-/H+ antiporter, was cloned from the halophyte Suaeda altissima. It belonged to the CLC family, comprising anionic channels and anion/H+ antiporters. SaCLCa1 ion specificity was studied by heterologous expression of this gen...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.152995
更新日期:2019-09-01 00:00:00
abstract::Since the presence of glyphosate in maize tissue cultures of proprietary line L2 was very detrimental to plant regeneration, we determined metabolic changes associated with the glyphosate effects on plant regeneration in maize cultures. The polar fraction composition and soluble and cell-wall-bound phenolics were anal...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.11.004
更新日期:2009-06-01 00:00:00
abstract::Chlorophyll-oxidase in barley thylakoids was activated by linolenic acid. The reaction products from endogenous chlorophylls were investigated by thin layer and high performance liquid chromatography. Within 2-10 min a new pigment was formed which migrated identically with authentic 13(2)-hydroxychlorophyll a (1a). Af...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80054-1
更新日期:1984-06-01 00:00:00
abstract::Needle differentiation is a very complex process associated with the formation of a mature photosynthetic organ. From meristem differentiation to leaf maturation, gene control must play an important role switching required genes on and off to define tissue functions, with the epigenetic code being one of the main regu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.09.018
更新日期:2010-03-15 00:00:00
abstract::The interactive effects of salinity and potassium deficiency on the growth, mineral elements and photosynthetic performance were investigated in wild (Hordeum maritimum L.) and cultivated barley (Hordeum vulgare L. var. Manel). At 28 d of growth, plants were treated with 3 mM K and 0 mM NaCl (3-0); 3 mM K and 100 mM N...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.06.013
更新日期:2009-12-15 00:00:00
abstract::In order to test an alternative selectable marker system for the production of transgenic peanut plants (Arachis hypogaea), the bacterial mercuric ion reductase gene, merA, was introduced into embryogenic cultures via microprojectile bombardment. MerA reduces toxic Hg(II) to the volatile and less toxic metallic mercur...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01087
更新日期:2003-08-01 00:00:00
abstract::Researchers have proven that nanomaterials have a significant effect on plant growth and development. To better understand the effects of nanomaterials on plants, Zhongshuang 11 was treated with different concentrations of graphene oxide. The results indicated that 25-100mg/l graphene oxide treatment resulted in short...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.02.011
更新日期:2016-04-01 00:00:00
abstract::Changes in phenolics (PhC - phenolic compounds) measured as UV-absorbing compounds (UVAC) and their localization as well as growth, lipid peroxidation (TBARS level) and proline (Pro) level in three-day-old roots of seedlings (T(o) stage) obtained from hydroprimed (H) and hydroprimed with melatonin (H-MEL) seeds after ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.08.011
更新日期:2012-01-01 00:00:00
abstract::Plant growth promoting rhizobacteria (PGPR) are extensively used as biofertilizers to improve the soil nutrition for a variety of crop plants. The plant-PGPR interaction, with special reference to chemical signalling molecules is not understood clearly, unlike other beneficial plant-microbe interactions. Chemo-attract...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153057
更新日期:2019-12-01 00:00:00
abstract::Nilaparvata lugens Stål, the brown planthopper (BPH), is one of the most destructive phloem-feeding insects of rice (Oryza sativa L.) throughout Asia. Here, we show that BPH feeding increases the level of endogenous nitric oxide (NO) in the leaf and sheath tissue of both resistant and susceptible rice cultivars. Howev...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.09.018
更新日期:2011-05-15 00:00:00
abstract::The plant vacuole constitutes a large transient storage compartment for nutrients, proteins and metabolites, and is a major cellular sink for toxic waste compounds. Amino acids can cross the vacuolar membrane via specific transport proteins, which are molecularly not well characterized. Two members of a small subfamil...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.11.012
更新日期:2014-05-01 00:00:00
abstract::This study demonstrates the impact of lead at hormetic (0.075 mM Pb(NO3)2) and sublethal (0.5 mM Pb(NO3)2) doses on the intensity of oxidative stress in pea seedlings (Pisum sativum L. cv. 'Cysterski'). Our first objective was to determine how exposure of pea seedlings to Pb alters the plant defence responses to pea a...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.152996
更新日期:2019-09-01 00:00:00
abstract::Blueberry (Vaccinium sp.) is thought to display a preference for the ammonium (NH4+) form over the nitrate (NO3-) form of inorganic nitrogen (N). This N-source preference has been associated with a generally low capacity to assimilate the NO3- form of N, especially within the shoot tissues. Nitrate assimilation is med...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.05.014
更新日期:2017-09-01 00:00:00
abstract::Citrus canker caused by Xanthomonas axonopodis pv. citri (Xac) is a devastating bacterial disease threatening the citrus industry. Salicylic acid (SA) plays a key role in plant defense response to biotic stress, but information is scarce concerning the application of SA to enhancing Xac resistance. In the present rese...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.03.018
更新日期:2012-08-15 00:00:00