Characterization of starch phosphorylase from the marine green microalga (Chlorophyta) Tetraselmis subcordiformis reveals its potential role in starch biosynthesis.

Abstract:

:In a marine green starch-producing microalga Tetraselmis subcordiformis, the role of starch phosphorylase (SP) in the starch biosynthesis was disclosed by characterizing the enzyme properties and activity variations during the starch accumulation process. TsSP4, a SP isoform accounting for the major SP activity in T. subcordiformis, was unique to be active in a monomer form with a molecular weight of approximately 110kDa. It resembled one of the chloroplast-located SPs (PhoA) in Chlamydomonas reinhardtii with a similarity of 63.3% in sequence, though it possessed the typical L78/80 domain found in the plastidial SPs (Pho1) of higher plants that was absent in PhoA. TsSP4 exhibited moderate sensitivity to ADP-Glc inhibition and had a high activity for longer-chain linear maltooligosacchride (MOS) and amylopectin against highly branched glycogen as the substrates. TsSP4 had 2-fold higher affinity for Glc-1-P in the synthetic direction than for Pi in the phosphorolytic direction, and the catalytic constant kcat for Glc-1-P was 2-fold of that for Pi. Collectively, TsSP4 preferred synthetic rather than phosphorolytic direction. TsSP4 could elongate MOSs even initially with Pi alone in the absence of Glc-1-P, which further supported its synthetic role in the starch biosynthesis. TsSP4 displayed increased activities in the developing and mature stage of starch biosynthesis under nitrogen-starvation conditions, indicating its possible contribution to the amylopectin amplification.

journal_name

J Plant Physiol

authors

Jiang J,Yao C,Cao X,Liu Y,Xue S

doi

10.1016/j.jplph.2017.07.019

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

84-93

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(17)30201-8

journal_volume

218

pub_type

杂志文章
  • Wide Dynamic Range Vegetation Index for remote quantification of biophysical characteristics of vegetation.

    abstract::The Normalized Difference Vegetation Index (NDVI) is widely used for monitoring, analyzing, and mapping temporal and spatial distributions of physiological and biophysical characteristics of vegetation. It is well documented that the NDVI approaches saturation asymptotically under conditions of moderate-to-high aboveg...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1078/0176-1617-01176

    authors: Gitelson AA

    更新日期:2004-02-01 00:00:00

  • Quality assessment of urban trees: a comparative study of physiological characterisation, airborne imaging and on site fluorescence monitoring by the OJIP-test.

    abstract::The purpose of this paper is to demonstrate the complementary utility of chlorophyll fast fluorescence OJIP transient (from 50 microns to 1s) measurements in the aerial study of rows of trees. We identify limitations in photochemical events induced by urban injuries on Platanus acerfolia L. using the JIP-test procedur...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1078/0176-1617-00917

    authors: Hermans C,Smeyers M,Rodriguez RM,Eyletters M,Strasser RJ,Delhaye JP

    更新日期:2003-01-01 00:00:00

  • Nitric oxide reduces Cu toxicity and Cu-induced NH4+ accumulation in rice leaves.

    abstract::Nitric oxide (NO) is a highly reactive, membrane-permeable free radical, which has recently emerged as an important antioxidant. Here we investigated the protective effect of NO against the toxicity and NH4+ accumulation in rice leaves caused by excess CuSO4 (10mmol L(-1)). It was found that free radical scavengers (s...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.02.003

    authors: Yu CC,Hung KT,Kao CH

    更新日期:2005-12-01 00:00:00

  • Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress.

    abstract::The relationships among photosynthetic acclimation, proline (Pro), soluble sugar (SS), and anthocyanin (An) accumulation in Arabidopsis thaliana leaves to the onset of drought stress (OnDS), mild (MiDS) and moderate drought stress (MoDS), were evaluated. As leaf water content (LWC) decreased, metabolic concentrations ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2011.12.015

    authors: Sperdouli I,Moustakas M

    更新日期:2012-04-15 00:00:00

  • Contribution of momilactone A and B to rice allelopathy.

    abstract::Eight cultivars of rice (Oryza sativa L.) inhibited shoot and root growth of Echinochloa crus-galli when co-cultured with rice seedlings in a bioassay medium. Momilactone A and B were found in the bioassay medium of all rice cultivars, and concentrations of momilactone A and B in the medium were 0.21-1.5 and 0.66-3.8 ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2010.01.014

    authors: Kato-Noguchi H,Hasegawa M,Ino T,Ota K,Kujime H

    更新日期:2010-07-01 00:00:00

  • Elicitation of galanthamine biosynthesis by Leucojum aestivum liquid shoot cultures.

    abstract::The effects of methyl jasmonate and jasmonic acid on galanthamine production, phenolic acid content and growth of Leucojum aestivum L. shoot culture, cultivated in submerged conditions were investigated. The best time-point for addition of elicitors was during the exponential phase of the culture growth. The maximal c...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2013.03.017

    authors: Ivanov I,Georgiev V,Pavlov A

    更新日期:2013-08-15 00:00:00

  • The spaceflight environment can induce transpositional activation of multiple endogenous transposable elements in a genotype-dependent manner in rice.

    abstract::Spaceflight represents a unique environmental condition whereby dysregulated gene expression and genomic instability can be provoked. However, detailed molecular characterization of the nature of genetic changes induced by spaceflight is yet to be documented in a higher eukaryote. Transposable elements (TEs) are ubiqu...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2009.06.007

    authors: Long L,Ou X,Liu J,Lin X,Sheng L,Liu B

    更新日期:2009-12-15 00:00:00

  • Cucumber carbohydrate metabolism and translocation under chilling night temperature.

    abstract::A cold-tolerant line (NY-1) and a cold-sensitive cultivar (Jinyan 4) of cucumber (Cucumis sativus) were treated with temperatures of 28 degrees C/22 degrees C or 28 degrees C/12 degrees C (day/night) in a 10-h photoperiod. Carbohydrates and related enzymes were assayed from 0 to 4 h after the start of the dark period....

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2006.02.005

    authors: Miao M,Xu X,Chen X,Xue L,Cao B

    更新日期:2007-05-01 00:00:00

  • Changes in the nature of phenolic deposits after re-warming as a result of melatonin pre-sowing treatment of Vigna radiata seeds.

    abstract::Changes in phenolics (PhC - phenolic compounds) measured as UV-absorbing compounds (UVAC) and their localization as well as growth, lipid peroxidation (TBARS level) and proline (Pro) level in three-day-old roots of seedlings (T(o) stage) obtained from hydroprimed (H) and hydroprimed with melatonin (H-MEL) seeds after ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2011.08.011

    authors: Szafrańska K,Glińska S,Janas KM

    更新日期:2012-01-01 00:00:00

  • Differential regulation of tomato ethylene responsive factor LeERF3b, a putative repressor, and the activator Pti4 in ripening mutants and in response to environmental stresses.

    abstract::Ethylene responsive transcription factors (ERFs) can be grouped into different classes with either gene activator or repressor activity. We have isolated a tomato ERF cDNA clone (LeERF3b) with sequence similarity to class II (repressor class) of the ERF family, which is regulated differently from Pti4 (a tomato ERF do...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2007.03.006

    authors: Chen G,Hu Z,Grierson D

    更新日期:2008-04-18 00:00:00

  • Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat.

    abstract::The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, s...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2009.06.017

    authors: Timotijević GS,Milisavljević MDj,Radović SR,Konstantinović MM,Maksimović VR

    更新日期:2010-01-01 00:00:00

  • Slow anion channel GhSLAC1 is essential for stomatal closure in response to drought stress in cotton.

    abstract::Drought is one of the abiotic stresses which affects the growth and development of plants, including cotton. The role of stomatal anion channel SLAC1 has been well established in regulating stomatal closure in response to drought stress in several plant species. However, the gene encoding for the main S-type anion cha...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2020.153360

    authors: Ren H,Su Q,Hussain J,Tang S,Song W,Sun Y,Liu H,Qi G

    更新日期:2021-01-10 00:00:00

  • Separation of abscission zone cells in detached Azolla roots depends on apoplastic pH.

    abstract::In studies on the mechanism of cell separation during abscission, little attention has been paid to the apoplastic environment. We found that the apoplastic pH surrounding abscission zone cells in detached roots of the water fern Azolla plays a major role in cell separation. Abscission zone cells of detached Azolla ro...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2012.08.008

    authors: Fukuda K,Yamada Y,Miyamoto K,Ueda J,Uheda E

    更新日期:2013-01-01 00:00:00

  • HPLC screening of natural vitamin E from mediterranean plant biofactories--a basic tool for pilot-scale bioreactors production of alpha-tocopherol.

    abstract::The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-spe...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.04.018

    authors: Sivakumar G,Bacchetta L,Gatti R,Zappa G

    更新日期:2005-11-01 00:00:00

  • Variable expansin expression in Arabidopsis leads to different growth responses.

    abstract::Expansins have long been implicated in the control of cell wall extensibility. However, despite ample evidence supporting a role for these proteins in the endogenous mechanism of plant growth, there are also examples in the literature where the outcome of altered expansin gene expression is difficult to reconcile with...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2013.09.009

    authors: Goh HH,Sloan J,Malinowski R,Fleming A

    更新日期:2014-02-15 00:00:00

  • Expression profiling of two stress-inducible genes encoding for miraculin-like proteins in citrus plants under insect infestation or salinity stress.

    abstract::The expression of two genes, namely Mir1 and Mir3 and the abundance of their encoded proteins, the putative miraculin-like proteins, MLP1 and MLP3, showing similarity to the Kunitz family of protease inhibitors, were monitored in the leaves of the citrus variety, 'Clementine' after Tetranychus urticae infestation and ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2013.08.001

    authors: Podda A,Simili M,Del Carratore R,Mouhaya W,Morillon R,Maserti BE

    更新日期:2014-01-01 00:00:00

  • Activity of a KATP+ channel in Arum spadix mitochondria during thermogenesis.

    abstract::This report demonstrates that mitochondria isolated from thermogenic Arum spadices possess an ATP-sensitive potassium channel--responsible for electrical potential (DeltaPsi) collapse and mitochondrial swelling--whose characteristics are similar to those previously described in pea and wheat mitochondria. In order to ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2007.10.012

    authors: Petrussa E,Casolo V,Peresson C,Krajnáková J,Macrì F,Vianello A

    更新日期:2008-09-08 00:00:00

  • Roles of hydroxyproline-rich glycoproteins in the pollen tube and style cell growth of tobacco (Nicotiana tabacum L.).

    abstract::Hydroxyproline-rich glycoproteins (HRGPs) are plant cell wall proteins related to plant growth and development, and extensins (EXTs) are a subfamily of HRGPs. In this study, the function of HRGPs, especially EXTs, was investigated in the pollen tube and style cell growth of Nicotiana tabacum L. By using the techniques...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2014.02.010

    authors: Zhang X,Ma H,Qi H,Zhao J

    更新日期:2014-07-15 00:00:00

  • Endogenous free polyamines and their role in fruit set of low and high parthenocarpic ability citrus cultivars.

    abstract::Endogenous free polyamines (PAs), putrescine, spermidine and spermine, from developing fruitlets of Citrus species (Citrus unshiu Marc. and Citrus clementina Hort ex Tanaka) which differ in their parthenocarpic ability, and from uniflowered leafy and leafless inflorescences differing in their ability to set, have been...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.01.011

    authors: Arias M,Carbonell J,Agustí M

    更新日期:2005-08-01 00:00:00

  • Changes in redox regulation during transition from C3 to single cell C4 photosynthesis in Bienertia sinuspersici.

    abstract::Bienertia sinuspersici performs single cell C4 photosynthesis without Kranz anatomy. Peripheral and central cytoplasmic compartments in a single chlorenchyma cell act as mesophyll cells and bundle sheath cells. Development of this specialized mechanism is gradual during plant development. Young leaves perform C3 photo...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.10.006

    authors: Uzilday B,Ozgur R,Yalcinkaya T,Turkan I,Sekmen AH

    更新日期:2018-01-01 00:00:00

  • PDH45 transgenic rice maintain cell viability through lower accumulation of Na(+), ROS and calcium homeostasis in roots under salinity stress.

    abstract::Salinity severely affects the growth/productivity of rice, which is utilized as major staple food crop worldwide. PDH45 (pea DNA helicase 45), a member of the DEAD-box helicase family, actively provides salinity stress tolerance, but the mechanism behind this is not well known. Therefore, in order to understand the me...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2015.11.008

    authors: Nath M,Yadav S,Kumar Sahoo R,Passricha N,Tuteja R,Tuteja N

    更新日期:2016-02-01 00:00:00

  • Drought tolerance in alfalfa (Medicago sativa L.) varieties is associated with enhanced antioxidative protection and declined lipid peroxidation.

    abstract::Drought stress is considered the most adverse factor restricting plant survival, growth, and productivity. The identification of the key adaptive mechanisms to drought stress is essential to enhance the drought resistance of plants. In this study, differential responses of three alfalfa varieties to drought, including...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2018.10.023

    authors: Zhang C,Shi S,Liu Z,Yang F,Yin G

    更新日期:2019-01-01 00:00:00

  • Hydrogen peroxide mediates defence responses induced by chitosans of different molecular weights in rice.

    abstract::To investigate the mechanisms whereby treatment with chitosan (CHN) is observed to increase the capacity of plants to resist pathogens, CHNs of different molecular weights (MWs) prepared by enzyme hydrolysis were used to treat rice cells in suspension culture and also rice seedlings. The results obtained with cultured...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2004.10.003

    authors: Lin W,Hu X,Zhang W,Rogers WJ,Cai W

    更新日期:2005-08-01 00:00:00

  • Application of a genome walking method for the study of the spinach Lhcb1 multigene family.

    abstract::We describe the application of a novel genome walking (GW) strategy for the one-shot identification of members of multigene families. The method was used to study the spinach Lhcb1 family (encoding the light harvesting complex protein Lhcb1), for which three cDNAs were known. Two additional genes and regulatory region...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2009.06.020

    authors: Leoni C,Volpicella M,Placido A,Gallerani R,Ceci LR

    更新日期:2010-01-15 00:00:00

  • Phospholipids profile in chloroplasts of Coffea spp. genotypes differing in cold acclimation ability.

    abstract::Environmental temperature change may induce modifications in membrane lipid properties and composition, which account for different physiological responses among plant species. Coffee plants, as many tropical species, are particularly sensitive to cold, but genotypes can present differences that can be exploited to im...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2013.07.007

    authors: Scotti-Campos P,Pais IP,Partelli FL,Batista-Santos P,Ramalho JC

    更新日期:2014-02-15 00:00:00

  • Variation of carbon and isotope natural abundances (δ15N and δ13C) of whole-plant sweet potato (Ipomoea batatas L.) subjected to prolonged water stress.

    abstract::Sweet potato (Ipomoea batatas L.) is an important crop in the world, cultivated in temperate climates under low inputs. Drought changes the plant biomass allocation, together with the carbon and nitrogen isotopic composition (δ13C and δ15N), whose changes are faintly known in sweet potato crops. Here, we show the biom...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2019.153052

    authors: Gouveia CSS,Ganança JFT,Slaski J,Lebot V,Pinheiro de Carvalho MÂA

    更新日期:2019-12-01 00:00:00

  • Amino sugars: new inhibitors of zeaxanthin epoxidase, a violaxanthin cycle enzyme.

    abstract::The effect of three sugars and their amino derivatives on violaxanthin cycle enzymes activity was investigated in duckweed (Lemna trisulca), a model water-plant. No effect of sugars and amino sugars on violaxanthin de-epoxidase was observed independent of incubation time; however, epoxidation of zeaxanthin to violaxan...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2006.09.003

    authors: Latowski D,Banaś AK,Strzałka K,Gabryś H

    更新日期:2007-03-01 00:00:00

  • Potassium nitrate application alleviates sodium chloride stress in winter wheat cultivars differing in salt tolerance.

    abstract::A sand culture experiment was conducted to answer the question whether or not exogenous KNO(3) can alleviate adverse effects of salt stress in winter wheat by monitoring plant growth, K(+)/Na(+) accumulation and the activity of some antioxidant enzymes. Seeds of two wheat cultivars (CVs), DK961 (salt-tolerant) and JN1...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2008.01.001

    authors: Zheng Y,Jia A,Ning T,Xu J,Li Z,Jiang G

    更新日期:2008-09-29 00:00:00

  • Starch deposition and amylase accumulation during somatic embryogenesis in bamboo (Dendrocalamus hamiltonii).

    abstract::In monocots, the zygotic embryo is protected and nourished by an endosperm. In the present study starch deposition and amylase accumulation was noticed during somatic embryogenesis in stem callus of a bamboo, Dendrocalamus hamiltonii. SEM studies revealed that starch grains were clearly visible in the scutellum during...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1078/0176-1617-01219

    authors: Godbole S,Sood A,Sharma M,Nagar PK,Ahuja PS

    更新日期:2004-02-01 00:00:00

  • Abscisic Acid-related changes in composition and physical state of membranes in bean leaves.

    abstract::Leaf discs of bean, maize, and wheat were incubated in the presence of either 2-(14)C mevalonic acid or methyl-(14)C choline chloride and varying concentrations of abscisic acid. Abscisic acid inhibited, dependent on concentration, the incorporation of mevalonate into free sterols and stimulated the incorporation of c...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(85)80197-8

    authors: Farkas T,Singh B,Nemecz G

    更新日期:1985-03-01 00:00:00