Abstract：
:We analyzed the cell wall proteome of lignifying suspension cell cultures (SCCs) from four gymnosperms that differ in evolution degree. This analysis showed the presence of "peptide sequence tags" (PSTs) corresponding to glucan endo-1,3-beta-D-glucosidase, xyloglucan-endotrans-glucosylase/hydrolase, chitinases, thaumatin-like proteins and proteins involved in lignin/lignan biosynthesis, such as dirigent-like proteins and peroxidases. Surprisingly, and given the abundance of peroxidases in the cell wall proteome of these gymnosperms, PSTs corresponding to peroxidases were only detected in tryptic fragments of the cell wall proteome of Cycas revoluta. The current lack of knowledge regarding C. revoluta peroxidases led us to purify, characterize and partially sequence the peroxidases responsible for lignin biosynthesis in this species. This yielded three peroxidase-enriched fractions: CrPrx 1, CrPrx 2 and CrPrx 3. Analyses of tryptic peptides of CrPrx 2 (32kDa) and CrPrx 3 (26kDa) suggest that CrPrx 3 arises from CrPrx 2 by protein truncation, and that CrPrx 3 apparently constitutes a post-translational modification of CrPrx 2. That CrPrx 2 and CrPrx 3 are apparently the same enzyme was also deduced from the similarity between the k(cat) shown by both peroxidases for the three monolignols. These results emphasize the analogies between the cell wall proteome of gymnosperms and angiosperms, the complexity of the peroxidase proteome, and the difficulties involved in establishing fine structure-function relationships.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Uzal EN,Gómez-Ros LV,Hernández JA,Pedreño MA,Cuello J,Ros Barceló Adoi
10.1016/j.jplph.2008.11.009subject
Has Abstractpub_date
2009-05-15 00:00:00pages
831-43issue
8eissn
0176-1617issn
1618-1328pii
S0176-1617(08)00358-1journal_volume
166pub_type
杂志文章abstract：:A sand culture experiment was conducted to answer the question whether or not exogenous KNO(3) can alleviate adverse effects of salt stress in winter wheat by monitoring plant growth, K(+)/Na(+) accumulation and the activity of some antioxidant enzymes. Seeds of two wheat cultivars (CVs), DK961 (salt-tolerant) and JN1...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.01.001
更新日期：2008-09-29 00:00:00
abstract：:The Basidiomycete fungus Ustilago maydis is the common agent of corn smut and is capable of inducing gall growth on infected tissue of the C4 plant maize (Zea mays). While U. maydis is very well characterized on the genetic level, the physiological changes in the host plant in response to U. maydis infection have not ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.05.008
更新日期：2008-01-01 00:00:00
abstract：:The effects of placing solid implants containing Fe sulfate in branches of Fe-deficient pear and peach trees on the composition of the xylem sap have been studied. Iron sulfate implants are commercially used in northeastern Spain to control iron chlorosis in fruit trees. Implants increased Fe concentrations and decrea...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01010
更新日期：2003-12-01 00:00:00
abstract：:We report in this study, the successful deployment of a double mutant acetolactate synthase gene (ALSdm, containing Pro 197 to Ser and Ser 653 to Asn substitutions) as an efficient in vitro selection marker for the development of transgenic plants in Brassica juncea (oilseed mustard). The ALS enzyme is inhibited by tw...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.02.001
更新日期：2004-09-01 00:00:00
abstract：:This study has been carried out to investigate the photosynthetic activities in developing embryos of Sesbania sesban under a highly osmotic environment. In S. sesban, the embryo turns green/chlorophyllous at the early heart shape stage. Interestingly, despite being deeply embedded within the supporting tissues (sever...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.03.002
更新日期：2004-10-01 00:00:00
abstract：:Grazing is accompanied by a multitude of processes including wounding, saliva deposition, and defoliation. Previous studies have focused on the effects of the grazing or clipping intensity on plant regrowth, survival, and composition in the grassland. However, the impact of saliva deposition on plants is poorly unders...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.08.012
更新日期：2011-03-15 00:00:00
abstract：:The functional activities of the photosynthetic apparatus of two tomato cultivars of different thermotolerance were investigated after a short period of high temperature treatment. Seedlings of two tomato genotypes, Lycopersicon esculentum var. Campbell-28 and the wild thermotolerant Nagcarlang, were grown under a pho...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.07.014
更新日期：2005-03-01 00:00:00
abstract：:Calcineurin B-like (CBL) interacting protein kinase 15 (CIPK15) is a newly identified positive regulator which is critical to directing the O(2) deficiency signal to the sugar signaling cascade as part of Amy3D (representative Amy3 gene) regulation in rice. It is located upstream and probably contributes to reserve mo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.06.003
更新日期：2012-10-15 00:00:00
abstract：:Since the presence of glyphosate in maize tissue cultures of proprietary line L2 was very detrimental to plant regeneration, we determined metabolic changes associated with the glyphosate effects on plant regeneration in maize cultures. The polar fraction composition and soluble and cell-wall-bound phenolics were anal...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2008.11.004
更新日期：2009-06-01 00:00:00
abstract：:We isolated a homolog of the potential anti-apoptotic gene, defender against apoptotic death (DAD1) from gladiolus petals as full-length cDNA (GlDAD1), and investigated the relationship between its expression and the execution processes of programmed cell death (PCD) in senescing petals. RNA gel blotting showed that G...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.06.005
更新日期：2004-11-01 00:00:00
abstract：:Fig fruit is well-known for its attractive flavor, color, and nutritional and medicinal value. Anthocyanin contributes to the fruit's color and constitutes a high percentage of the total antioxidant content of the fig fruit. We quantified the major anthocyanins and characterized the expression levels of anthocyanin-bi...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2020.153192
更新日期：2020-08-01 00:00:00
abstract：:Linoleic acid (LA, C18:2) and α-linolenic acid (ALA, C18:3) are polyunsaturated fatty acids (PUFAs) and major storage compounds in plant seed oils. Microsomal ω-6 and ω-3 fatty acid (FA) desaturases catalyze the synthesis of seed oil LA and ALA, respectively. Jatropha curcas L. seed oils contain large proportions of L...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.04.019
更新日期：2013-10-15 00:00:00
abstract：:To elucidate influences on the tocopherol biosynthesis in cyanobacteria, wild type and mutant cells of a putative methyltransferase in tocopherol and plastoquinone biosynthesis of Synechocystis sp. PCC 6803 were grown under different conditions. The vitamin E content of cells grown under different light regimes, photo...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.04.006
更新日期：2005-07-01 00:00:00
abstract：:Characterization of the photosynthetic electron transport in a mutant of Spirulina platensis, generated by chemical mutagenesis, demonstrated that the electron transfer from the plastoquinone (PQ) to cytochrome b6/f was slowed. Thermoluminescence (TL) measurements suggested the presence of reversed energy flow via PQ,...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.11.012
更新日期：2005-10-01 00:00:00
abstract：:Two 9-cis-epoxycarotenoid dioxygenase (NCED) cDNAs have been cloned from a petal library of Gentiana lutea. Both cDNAs carry a putative transit sequence for chloroplast import and differ mainly in their length and the 5'-flanking regions. GlNCED1 was evolutionary closely related to Arabidopsis thaliana NCED6 whereas G...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.02.010
更新日期：2007-02-01 00:00:00
abstract：:CBFs (C-repeat binding factors) are transcription factors that are rapidly induced by low temperature and that recognize the CRT/DRE element in the promoter of a set of cold regulated genes, the CBF regulon. Dehydrins are proteins that accumulate in plants under stress conditions, such as low temperature, and some for...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.12.018
更新日期：2012-05-01 00:00:00
abstract：:Some intracellular organelles found in eukaryotes such as plants have arisen through the endocytotic engulfment of prokaryotic cells. This accounts for the presence of plant membrane intrinsic proteins that have homologs in prokaryotic cells. Other organelles, such as those of the endomembrane system, are thought to h...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.09.022
更新日期：2014-05-15 00:00:00
abstract：:Cell wall polysaccharides, pectin composition, as well as apoplastic superoxide dismutase and peroxidase activities were investigated in strawberry (Fragaria x ananassa) cultivars (cvs) Korona and Elsanta differing in their ozone sensitivity. Plants were exposed to 140-170 microg m(-3) ozone either short-term for 7 da...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.06.012
更新日期：2008-05-26 00:00:00
abstract：:Plant tissue growth can be regulated and controlled via culture media composition. A number of different laborious and time-consuming approaches have been used to attempt development of optimized media for a wide range of species and applications. However, plant tissue culture is a very complex task, and the identific...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.04.008
更新日期：2011-10-15 00:00:00
abstract：:Changes in phenolics (PhC - phenolic compounds) measured as UV-absorbing compounds (UVAC) and their localization as well as growth, lipid peroxidation (TBARS level) and proline (Pro) level in three-day-old roots of seedlings (T(o) stage) obtained from hydroprimed (H) and hydroprimed with melatonin (H-MEL) seeds after ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.08.011
更新日期：2012-01-01 00:00:00
abstract：:Retrograde signaling, defined as the signaling events leading from the plastids to the nucleus, coordinates the expression of plastid and nuclear genes and is crucial for metabolic as well as developmental processes of the plastids. In the recent past, the identification of various components that are involved in the ...
journal_title：Journal of plant physiology
pub_type： 杂志文章,评审
doi：10.1016/j.jplph.2015.04.001
更新日期：2015-06-01 00:00:00
abstract：:Flowering time control is important for fruit production in Fragaria × ananassa. The flowering inhibition pathway has been extensively elucidated in the woodland strawberry, Fragaria vesca, whereas the factors involved in its promotion remain unclear. In this study, we investigated the environmental responses of F. × ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.01.007
更新日期：2015-04-01 00:00:00
abstract：:Leaf water content is an important variable for understanding plant physiological properties. This study evaluates a spectral analysis approach, continuous wavelet analysis (CWA), for the spectroscopic estimation of leaf gravimetric water content (GWC, %) and determines robust spectral indicators of GWC across a wide ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2012.04.006
更新日期：2012-08-15 00:00:00
abstract：:Saliva miltiorrhiza ethylene response factor (SmERF), predicted to be expressed genome-wide, is the potential regulator of tanshinone biosynthesis. However, few studies have investigated its transcriptional regulation pathways in tanshinone biosynthesis. Here, we report an ethylene response factor (SmERF8), which was ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153006
更新日期：2020-01-01 00:00:00
abstract：:Melatonin (MEL; N-acetyl-5-methoxytryptamine) plays an important role in plant stress defense. Various plant species rich in this indoleamine have shown a higher capacity for stress tolerance. Moreover, it has great potential for plant biostimulation, is biodegradable and non-toxic for the environment. All this indica...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.01.012
更新日期：2016-04-01 00:00:00
abstract：:Ethephon, an ethylene releasing compound, promoted leaf senescence, H2O2 elevation, and senescence-associated gene expression in sweet potato. It also affected the glutathione and ascorbate levels, which in turn perturbed H2O2 homeostasis. The decrease of reduced glutathione and the accumulation of dehydroascorbate co...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.05.015
更新日期：2013-11-15 00:00:00
abstract：:Suppression of photorespiration by low O2 concentrations (Method 1) and simultaneous measurements of gas exchange and chlorophyll fluorescence (Method 2) are often used to estimate leaf photorespiration rate (Rp) of C3 plants. However, it is largely unknown whether Method 1 and Method 2 can be used equivalently in est...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.153002
更新日期：2019-09-01 00:00:00
abstract：:Protein metabolism and expression play important role in plant adaptation to water stress. The objectives of this study were to examine proteomic responses to water stress induced by polyethylene glycol (PEG) in creeping bentgrass (Agrostis stolonifera L.) leaves and to identify proteins associated with stress toleran...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.05.006
更新日期：2010-11-15 00:00:00
abstract：:Acidification of the starchy endosperm by the aleurone layer following germination has been established; however, the physiological and metabolic responses of this tissue to external pH have been incompletely investigated. In this investigation, isolated wheat (Triticum aestivum) aleurone layers were incubated in diff...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2004.05.010
更新日期：2004-12-01 00:00:00
abstract：:Arbuscular mycorrhizal fungal (AMF) colonization can induce both the local and the systemic increase in phenolic accumulation in hosts. However, the signaling molecules responsible for the systemic induction is still unclear. In this study, a split-root rhizobox system was designed to explore these molecules, with one...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.01.016
更新日期：2015-04-15 00:00:00
