Abstract:
:A Cu-binding protein was isolated from roots of the grass Agrostis gigantea Roth. Heat stable proteins were chromatographed on the strongly basic anion exchanger QAE-Sephadex A-25. Dark brown pigments were retained by the ion exchanger during elution of Cu-binding protein. Further purification was achieved by gel filtration on Bio-Gel P-6 in buffer containing 1 kmol m(-1) KCl. The resulting protein was a light blue powder; had an apparent molecular weight of 1700; contained 11% cys, 14% asp, 27% glu and 0.25g atoms Cu per mol; the Cu : cysteine ratio was 1:6. No Cu(I) typical of Cu(I)-thiolate bonds of Cu-thionein was found despite taking precautions to prevent Cu oxidation.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Rauser WEdoi
10.1016/S0176-1617(84)80061-9subject
Has Abstractpub_date
1984-05-01 00:00:00pages
143-52issue
2eissn
0176-1617issn
1618-1328pii
S0176-1617(84)80061-9journal_volume
115pub_type
杂志文章abstract::Raffinose synthase (RS, EC2.4.1.82) is one of the key enzymes that channels sucrose into the raffinose family oligosaccharides (RFOs) biosynthetic pathway. However, the gene encoding RS is poorly characterized in cucumber (Cucumis sativus L.), which is a typical RFOs-translocating plant species. Here we isolated the g...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.019
更新日期:2012-12-15 00:00:00
abstract::Currents flowing through slow vacuolar SV channels of rape (Brassica napus) growing on media supplemented with Cd²+ (400 μM), and/or SeO₄(²⁻) (2μM) were examined. The aim of the study was to investigate the role of Cd²+ in modulation of SV channel activity and to determine whether Se reverses the effect of cadmium. Va...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.06.016
更新日期:2010-12-15 00:00:00
abstract::PAM fluorometry showed that the orchid Vanda sp (Gaud ex Pfitzers, Vandeae) had photosynthetic electron transport yields in leaves reaching ≈ 0.617 ± 0.262 at midday. Yield decayed exponentially as irradiance increased (Y½ = 128 ± 12.4 μmol photon m-2 s-1). Optimum irradiance (Eopt) for ETR (Photosynthetic Electron Tr...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153187
更新日期:2020-08-01 00:00:00
abstract::Autophagy, a highly conserved intracellular degradation system, is regarded to be responsible for self-defense and protect cells from abiotic stress. Extensive studies have demonstrated that autophagy plays a crucial role in regulating plant growth and development as well as in response to diverse stresses. However, l...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.06.012
更新日期:2018-10-01 00:00:00
abstract::In Lilium longiflorum, vernalization is both an obligatory requirement and the major factor affecting flowering time, however, little is known about the molecular regulation of this mechanism in Lilium and other flowering bulbs. Exposure of L. longiflorum bulbs to 9 weeks at 4°C greatly promoted stem elongation within...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2014.09.015
更新日期:2015-01-15 00:00:00
abstract::Two 9-cis-epoxycarotenoid dioxygenase (NCED) cDNAs have been cloned from a petal library of Gentiana lutea. Both cDNAs carry a putative transit sequence for chloroplast import and differ mainly in their length and the 5'-flanking regions. GlNCED1 was evolutionary closely related to Arabidopsis thaliana NCED6 whereas G...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.02.010
更新日期:2007-02-01 00:00:00
abstract::A single polypeptide catalysing the two step conversion of orotate to UMP in cultured tomato cells was purified to near homogeneity as judged by analytical disc gel electrophoresis. After electrophoresis of the dodecyl sulphate denaturated enzyme one single protein band appeared from which both enzyme activities could...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80109-1
更新日期:1984-01-01 00:00:00
abstract::Plant-derived elicitor is a new type of plant vaccine developed in the contemporary era, and it has safe and broad application prospects in organic agriculture. Research on defense mechanisms triggered by elicitor has become a hot topic in recent years. The Chrysanthemum indicum polysaccharide (CIP) obtained by separa...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.153107
更新日期:2020-02-01 00:00:00
abstract::The involvement of the ascorbate (AsA) system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress was studied. The treatment of 5-day-old pumpkin seedlings with 50 microM aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumul...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.06.008
更新日期:2005-05-01 00:00:00
abstract::Nowadays, irrigation with low quality water is becoming an alternative to satisfy the needs of crops. However, some plant species have to deal with high salinity of reclaimed water, by adapting their physiological behaviour during both saline and recovery periods and developing morphological changes in their leaves. T...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2015.09.007
更新日期:2015-09-01 00:00:00
abstract::The effect of five topolins (meta-Topolin=mT; meta-Topolin riboside=mTR; meta-Methoxy topolin=MemT; meta-Methoxy topolin riboside=MemTR and 6-(meta-methoxy)-9-(tetrahydropyran-2-yl)-topolin=MemTTHP) on the photosynthetic pigments and leaf structures of micropropagated 'Williams' bananas was compared with the commonly ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.06.006
更新日期:2012-10-15 00:00:00
abstract::Plant natriuretic peptides (PNPs) belong to a novel class of peptidic signaling molecules that share some structural similarity to the N-terminal domain of expansins and affect physiological processes such as water and ion homeostasis at nano-molar concentrations. Here we show that a recombinant Arabidopsis thaliana P...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.03.011
更新日期:2011-09-15 00:00:00
abstract::Plants resistant to triazine-type herbicides are known to be altered in their photosystem II reaction center. Serine at site 264 in D1 protein is replaced by glycine. The measurements of chlorophyll a fluorescence excitations with a variable number of saturating flashes in Chenopodium album plants show characteristic ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.04.011
更新日期:2009-10-15 00:00:00
abstract::Leaf growth is one of the first physiological processes affected by changes in plant water status under drought. A decrease in leaf expansion rate usually precedes any reduction in stomatal conductance or photosynthesis. Changes in leaf size and stomatal opening are potential adaptive mechanisms, which may help avoid ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.03.003
更新日期:2010-09-01 00:00:00
abstract::Atmospheric CO2 levels and global temperatures are expected to rise in the next decades, and viticulture must face these changes. Within this context, exploiting the intra-varietal diversity of grapevine (Vitis vinifera L.) can be a useful tool for the adaptation of this crop to climate change. The aim of the present ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153226
更新日期:2020-09-01 00:00:00
abstract::Auxin is a well-known, crucial regulator of the entire plant lifecycle, not only orchestrating many aspects of plant growth and development, but also playing various roles in biotic and abiotic stress. This study reports the isolation and functional characterization of a DUF-966 domain-containing gene, At3g46110, re-n...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2019.152990
更新日期:2019-09-01 00:00:00
abstract::Plants counteract Cd toxicity by activating cellular stress responses. The simultaneous exogenous application of methyl jasmonate (MeJA) and methyl-β-cyclodextrin (CD) before Cd exposure improved the response of Arachis hypogaea hairy root culture to the unfavourable effects of Cd toxicity. At 24 h after elicitation, ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153278
更新日期:2020-11-01 00:00:00
abstract::The effects of three N(6)-substituted aminopurine derivatives containing either allenic or acetylenic side-chains on in vitro and in vivo cytokinin dehydrogenase (CKX; EC 1.5.99.12) activities were determined. At concentrations < or = 100 microM, the acetylenic derivative (HA-2) had no effect on in vitro CKX activity....
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.03.012
更新日期:2005-11-01 00:00:00
abstract::We have proposed that the polyamine spermine (Spm) functions as a signaling molecule to evoke defense reactions/cell death in avirulent pathogen-attacked tobacco plants. To understand its molecular basis in depth, Spm-responsive genes in Arabidopsis thaliana were identified by SuperSAGE analysis. Close to 90% of the S...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.08.006
更新日期:2009-04-01 00:00:00
abstract::Our previous study suggests that salicylic acid mediates tolerance in barley plants to paraquat (Ananieva et al. 2002). To further define the role of SA in paraquat induced responses, we analysed the capacity of the antioxidative defence system by measuring the activities of several antioxidative enzymes: superoxide d...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-01022
更新日期:2004-03-01 00:00:00
abstract::A non-destructive nitrogen (N) detector [Agriexpert PPW-3000 (PPW-3000)] is a useful device for rapid and non-destructive measurement of leaf N content. However, some studies find a poor correlation between the PPW-3000 reading and the actual leaf N content; the R2 value of the approximate equation was low. To improve...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.09.004
更新日期:2006-04-01 00:00:00
abstract::In our previous study we showed that volatile organic compounds (VOCs) from Alcaligenes faecalis JBCS1294 (JBCS1294) induced tolerance to salt stress in Arabidopsis thaliana by influencing the auxin and gibberellin pathways and upregulating the expression of key ion transporters. The aim of this study was to evaluate ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.04.002
更新日期:2017-07-01 00:00:00
abstract::This article reports marked modulation of the activity and regulatory properties of phosphoenolpyruvate carboxylase (PEPC) by temperature and light in leaf discs as well as leaves of Amaranthus hypochondriacus. The activity of PEPC increased by 1.7-fold at 45 degrees C over 25 degrees C. Warm temperature also stimulat...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.09.003
更新日期:2008-07-07 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) constitute a unique family of enzymes in plants that are characterized by a C-terminal calmodulin (CaM)-like domain. Through protein kinase assays, we have examined the levels of cucumber calcium-dependent kinase (CsCDPK) activity in various organs of cucumber seedlings and pl...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2003.11.010
更新日期:2004-08-01 00:00:00
abstract::Plant development displays an exceptional plasticity and adaptability that involves the dynamic, asymmetric distribution of the phytohormone auxin. Polar auxin flow, which requires transport facilitators of the PIN family, largely contributes to the establishment and maintenance of auxin gradients and mediates multipl...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.01.025
更新日期:2011-07-15 00:00:00
abstract::Chromium (Cr) contamination in soil is a growing concern in relation to sustainable agricultural production and food safety. Nitric oxide (NO) and, more recently, hydrogen sulfide (H2S) are considered to be new signalling molecules with biotechnological applications in the agronomical sector. Using 9-day-old maize (Ze...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.010
更新日期:2017-12-01 00:00:00
abstract::This study has been carried out to investigate the photosynthetic activities in developing embryos of Sesbania sesban under a highly osmotic environment. In S. sesban, the embryo turns green/chlorophyllous at the early heart shape stage. Interestingly, despite being deeply embedded within the supporting tissues (sever...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2004.03.002
更新日期:2004-10-01 00:00:00
abstract::Leaf discs of bean, maize, and wheat were incubated in the presence of either 2-(14)C mevalonic acid or methyl-(14)C choline chloride and varying concentrations of abscisic acid. Abscisic acid inhibited, dependent on concentration, the incorporation of mevalonate into free sterols and stimulated the incorporation of c...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(85)80197-8
更新日期:1985-03-01 00:00:00
abstract::The effect of subculture interval and total culture age on plant regeneration from serially subcultured, leaf derived embryogenic callus cultures of Pennisetum purpureum (Napier grass) was determined. During 38 weeks of culture both embryogenic callus proliferation and the yield of plants was maximal for callus from f...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80028-0
更新日期:1984-12-01 00:00:00
abstract::The distribution of the endogenous PA fractions throughout the entire perennial woody grapevine (Vitis vinifera L.) plant was studied, along with the expression profiles of the PA anabolic and catabolic genes and their substrates and secondary metabolites. Putrescine fractions increased with increasing leaf age, altho...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.03.013
更新日期:2009-09-15 00:00:00