Partial Purification and Characterization of Copper-binding Protein from Roots of Agrostis gigantea Roth.

Abstract:

:A Cu-binding protein was isolated from roots of the grass Agrostis gigantea Roth. Heat stable proteins were chromatographed on the strongly basic anion exchanger QAE-Sephadex A-25. Dark brown pigments were retained by the ion exchanger during elution of Cu-binding protein. Further purification was achieved by gel filtration on Bio-Gel P-6 in buffer containing 1 kmol m(-1) KCl. The resulting protein was a light blue powder; had an apparent molecular weight of 1700; contained 11% cys, 14% asp, 27% glu and 0.25g atoms Cu per mol; the Cu : cysteine ratio was 1:6. No Cu(I) typical of Cu(I)-thiolate bonds of Cu-thionein was found despite taking precautions to prevent Cu oxidation.

journal_name

J Plant Physiol

authors

Rauser WE

doi

10.1016/S0176-1617(84)80061-9

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

143-52

issue

2

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(84)80061-9

journal_volume

115

pub_type

杂志文章
  • Molecular cloning, characteristics and low temperature response of raffinose synthase gene in Cucumis sativus L.

    abstract::Raffinose synthase (RS, EC2.4.1.82) is one of the key enzymes that channels sucrose into the raffinose family oligosaccharides (RFOs) biosynthetic pathway. However, the gene encoding RS is poorly characterized in cucumber (Cucumis sativus L.), which is a typical RFOs-translocating plant species. Here we isolated the g...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2012.07.019

    authors: Sui XL,Meng FZ,Wang HY,Wei YX,Li RF,Wang ZY,Hu LP,Wang SH,Zhang ZX

    更新日期:2012-12-15 00:00:00

  • Cadmium and selenium modulate slow vacuolar channels in rape (Brassica napus) vacuoles.

    abstract::Currents flowing through slow vacuolar SV channels of rape (Brassica napus) growing on media supplemented with Cd²+ (400 μM), and/or SeO₄(²⁻) (2μM) were examined. The aim of the study was to investigate the role of Cd²+ in modulation of SV channel activity and to determine whether Se reverses the effect of cadmium. Va...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2010.06.016

    authors: Dziubinska H,Filek M,Krol E,Trebacz K

    更新日期:2010-12-15 00:00:00

  • Photosynthesis in a Vanda sp orchid with Photosynthetic Roots.

    abstract::PAM fluorometry showed that the orchid Vanda sp (Gaud ex Pfitzers, Vandeae) had photosynthetic electron transport yields in leaves reaching ≈ 0.617 ± 0.262 at midday. Yield decayed exponentially as irradiance increased (Y½ = 128 ± 12.4 μmol photon m-2 s-1). Optimum irradiance (Eopt) for ETR (Photosynthetic Electron Tr...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2020.153187

    authors: Sma-Air S,Ritchie RJ

    更新日期:2020-08-01 00:00:00

  • Genome-wide sequence and expressional analysis of autophagy Gene family in bread wheat (Triticum aestivum L.).

    abstract::Autophagy, a highly conserved intracellular degradation system, is regarded to be responsible for self-defense and protect cells from abiotic stress. Extensive studies have demonstrated that autophagy plays a crucial role in regulating plant growth and development as well as in response to diverse stresses. However, l...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2018.06.012

    authors: Yue W,Nie X,Cui L,Zhi Y,Zhang T,Du X,Song W

    更新日期:2018-10-01 00:00:00

  • Characterization of expressed sequence tags from Lilium longiflorum in vernalized and non-vernalized bulbs.

    abstract::In Lilium longiflorum, vernalization is both an obligatory requirement and the major factor affecting flowering time, however, little is known about the molecular regulation of this mechanism in Lilium and other flowering bulbs. Exposure of L. longiflorum bulbs to 9 weeks at 4°C greatly promoted stem elongation within...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2014.09.015

    authors: Lugassi-Ben Hamo M,Martin CV,Zaccai M

    更新日期:2015-01-15 00:00:00

  • Cloning of two individual cDNAS encoding 9-cis-epoxycarotenoid dioxygenase from Gentiana lutea, their tissue-specific expression and physiological effect in transgenic tobacco.

    abstract::Two 9-cis-epoxycarotenoid dioxygenase (NCED) cDNAs have been cloned from a petal library of Gentiana lutea. Both cDNAs carry a putative transit sequence for chloroplast import and differ mainly in their length and the 5'-flanking regions. GlNCED1 was evolutionary closely related to Arabidopsis thaliana NCED6 whereas G...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2006.02.010

    authors: Zhu C,Kauder F,Römer S,Sandmann G

    更新日期:2007-02-01 00:00:00

  • Evidence that a Single Polypeptide Catalyses the Two Step Conversion of Orotate to UMP in Cells from a Tomato Suspension Culture.

    abstract::A single polypeptide catalysing the two step conversion of orotate to UMP in cultured tomato cells was purified to near homogeneity as judged by analytical disc gel electrophoresis. After electrophoresis of the dodecyl sulphate denaturated enzyme one single protein band appeared from which both enzyme activities could...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(84)80109-1

    authors: Walther R,Wald K,Glund K,Tewes A

    更新日期:1984-01-01 00:00:00

  • CIP elicitors on the defense response of A. macrocephala and its related gene expression analysis.

    abstract::Plant-derived elicitor is a new type of plant vaccine developed in the contemporary era, and it has safe and broad application prospects in organic agriculture. Research on defense mechanisms triggered by elicitor has become a hot topic in recent years. The Chrysanthemum indicum polysaccharide (CIP) obtained by separa...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2019.153107

    authors: Zhang P,Zheng F,Chen L,Lu X,Tian W

    更新日期:2020-02-01 00:00:00

  • Changes in the ascorbate system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress.

    abstract::The involvement of the ascorbate (AsA) system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress was studied. The treatment of 5-day-old pumpkin seedlings with 50 microM aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumul...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2004.06.008

    authors: Dipierro N,Mondelli D,Paciolla C,Brunetti G,Dipierro S

    更新日期:2005-05-01 00:00:00

  • Influence of arbuscular mycorrhizal fungi and treated wastewater on water relations and leaf structure alterations of Viburnum tinus L. plants during both saline and recovery periods.

    abstract::Nowadays, irrigation with low quality water is becoming an alternative to satisfy the needs of crops. However, some plant species have to deal with high salinity of reclaimed water, by adapting their physiological behaviour during both saline and recovery periods and developing morphological changes in their leaves. T...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2015.09.007

    authors: Gómez-Bellot MJ,Nortes PA,Ortuño MF,Romero C,Fernández-García N,Sánchez-Blanco MJ

    更新日期:2015-09-01 00:00:00

  • The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated 'Williams' bananas.

    abstract::The effect of five topolins (meta-Topolin=mT; meta-Topolin riboside=mTR; meta-Methoxy topolin=MemT; meta-Methoxy topolin riboside=MemTR and 6-(meta-methoxy)-9-(tetrahydropyran-2-yl)-topolin=MemTTHP) on the photosynthetic pigments and leaf structures of micropropagated 'Williams' bananas was compared with the commonly ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2012.06.006

    authors: Aremu AO,Bairu MW,Szüčová L,Finnie JF,Van Staden J

    更新日期:2012-10-15 00:00:00

  • The Arabidopsis thaliana natriuretic peptide AtPNP-A is a systemic regulator of leaf dark respiration and signals via the phloem.

    abstract::Plant natriuretic peptides (PNPs) belong to a novel class of peptidic signaling molecules that share some structural similarity to the N-terminal domain of expansins and affect physiological processes such as water and ion homeostasis at nano-molar concentrations. Here we show that a recombinant Arabidopsis thaliana P...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2011.03.011

    authors: Ruzvidzo O,Donaldson L,Valentine A,Gehring C

    更新日期:2011-09-15 00:00:00

  • Higher concentration of Q(B)-nonreducing photosystem II centers in triazine-resistant Chenopodium album plants as revealed by analysis of chlorophyll fluorescence kinetics.

    abstract::Plants resistant to triazine-type herbicides are known to be altered in their photosystem II reaction center. Serine at site 264 in D1 protein is replaced by glycine. The measurements of chlorophyll a fluorescence excitations with a variable number of saturating flashes in Chenopodium album plants show characteristic ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2009.04.011

    authors: Van Rensen JJ,Vredenberg WJ

    更新日期:2009-10-15 00:00:00

  • Broader leaves result in better performance of indica rice under drought stress.

    abstract::Leaf growth is one of the first physiological processes affected by changes in plant water status under drought. A decrease in leaf expansion rate usually precedes any reduction in stomatal conductance or photosynthesis. Changes in leaf size and stomatal opening are potential adaptive mechanisms, which may help avoid ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2010.03.003

    authors: Farooq M,Kobayashi N,Ito O,Wahid A,Serraj R

    更新日期:2010-09-01 00:00:00

  • Growth performance and carbon partitioning of grapevine Tempranillo clones under simulated climate change scenarios: Elevated CO2 and temperature.

    abstract::Atmospheric CO2 levels and global temperatures are expected to rise in the next decades, and viticulture must face these changes. Within this context, exploiting the intra-varietal diversity of grapevine (Vitis vinifera L.) can be a useful tool for the adaptation of this crop to climate change. The aim of the present ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2020.153226

    authors: Arrizabalaga-Arriazu M,Morales F,Irigoyen JJ,Hilbert G,Pascual I

    更新日期:2020-09-01 00:00:00

  • Characterization the role of a UFC homolog, AtAuxRP3, in the regulation of Arabidopsis seedling growth and stress response.

    abstract::Auxin is a well-known, crucial regulator of the entire plant lifecycle, not only orchestrating many aspects of plant growth and development, but also playing various roles in biotic and abiotic stress. This study reports the isolation and functional characterization of a DUF-966 domain-containing gene, At3g46110, re-n...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2019.152990

    authors: Shen L,Zhong T,Wang L,Zhang Q,Jin H,Xu M,Ye J

    更新日期:2019-09-01 00:00:00

  • Enhancement of adaptive response in peanut hairy root by exogenous signalling molecules under cadmium stress.

    abstract::Plants counteract Cd toxicity by activating cellular stress responses. The simultaneous exogenous application of methyl jasmonate (MeJA) and methyl-β-cyclodextrin (CD) before Cd exposure improved the response of Arachis hypogaea hairy root culture to the unfavourable effects of Cd toxicity. At 24 h after elicitation, ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2020.153278

    authors: Pilaisangsuree V,Anuwan P,Supdensong K,Lumpa P,Kongbangkerd A,Limmongkon A

    更新日期:2020-11-01 00:00:00

  • Mechanism-based irreversible inhibitors of cytokinin dehydrogenase.

    abstract::The effects of three N(6)-substituted aminopurine derivatives containing either allenic or acetylenic side-chains on in vitro and in vivo cytokinin dehydrogenase (CKX; EC 1.5.99.12) activities were determined. At concentrations < or = 100 microM, the acetylenic derivative (HA-2) had no effect on in vitro CKX activity....

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.03.012

    authors: Suttle JC,Mornet R

    更新日期:2005-11-01 00:00:00

  • Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus.

    abstract::We have proposed that the polyamine spermine (Spm) functions as a signaling molecule to evoke defense reactions/cell death in avirulent pathogen-attacked tobacco plants. To understand its molecular basis in depth, Spm-responsive genes in Arabidopsis thaliana were identified by SuperSAGE analysis. Close to 90% of the S...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2008.08.006

    authors: Mitsuya Y,Takahashi Y,Berberich T,Miyazaki A,Matsumura H,Takahashi H,Terauchi R,Kusano T

    更新日期:2009-04-01 00:00:00

  • Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to paraquat.

    abstract::Our previous study suggests that salicylic acid mediates tolerance in barley plants to paraquat (Ananieva et al. 2002). To further define the role of SA in paraquat induced responses, we analysed the capacity of the antioxidative defence system by measuring the activities of several antioxidative enzymes: superoxide d...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1078/0176-1617-01022

    authors: Ananieva EA,Christov KN,Popova LP

    更新日期:2004-03-01 00:00:00

  • Accurate estimation of nitrogen concentration in deciduous tree leaves in a field study using a portable non-destructive nitrogen detector.

    abstract::A non-destructive nitrogen (N) detector [Agriexpert PPW-3000 (PPW-3000)] is a useful device for rapid and non-destructive measurement of leaf N content. However, some studies find a poor correlation between the PPW-3000 reading and the actual leaf N content; the R2 value of the approximate equation was low. To improve...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2005.09.004

    authors: Eguchi N,Ichie T,Ji D,Karatsu K,Koike T

    更新日期:2006-04-01 00:00:00

  • A cocktail of volatile compounds emitted from Alcaligenes faecalis JBCS1294 induces salt tolerance in Arabidopsis thaliana by modulating hormonal pathways and ion transporters.

    abstract::In our previous study we showed that volatile organic compounds (VOCs) from Alcaligenes faecalis JBCS1294 (JBCS1294) induced tolerance to salt stress in Arabidopsis thaliana by influencing the auxin and gibberellin pathways and upregulating the expression of key ion transporters. The aim of this study was to evaluate ...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.04.002

    authors: Bhattacharyya D,Lee YH

    更新日期:2017-07-01 00:00:00

  • Mutual stimulation of temperature and light effects on C(4) phosphoenolpyruvate carboxylase in leaf discs and leaves of Amaranthus hypochondriacus.

    abstract::This article reports marked modulation of the activity and regulatory properties of phosphoenolpyruvate carboxylase (PEPC) by temperature and light in leaf discs as well as leaves of Amaranthus hypochondriacus. The activity of PEPC increased by 1.7-fold at 45 degrees C over 25 degrees C. Warm temperature also stimulat...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2007.09.003

    authors: Avasthi UK,Raghavendra AS

    更新日期:2008-07-07 00:00:00

  • Variations in the level of enzyme activity and immunolocalization of calcium-dependent protein kinases in the phloem of different cucumber organs.

    abstract::Calcium-dependent protein kinases (CDPKs) constitute a unique family of enzymes in plants that are characterized by a C-terminal calmodulin (CaM)-like domain. Through protein kinase assays, we have examined the levels of cucumber calcium-dependent kinase (CsCDPK) activity in various organs of cucumber seedlings and pl...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2003.11.010

    authors: Kumar KG,Jayabaskaran C

    更新日期:2004-08-01 00:00:00

  • Narciclasine modulates polar auxin transport in Arabidopsis roots.

    abstract::Plant development displays an exceptional plasticity and adaptability that involves the dynamic, asymmetric distribution of the phytohormone auxin. Polar auxin flow, which requires transport facilitators of the PIN family, largely contributes to the establishment and maintenance of auxin gradients and mediates multipl...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2011.01.025

    authors: Na X,Hu Y,Yue K,Lu H,Jia P,Wang H,Wang X,Bi Y

    更新日期:2011-07-15 00:00:00

  • Alleviation of Cr(VI)-induced oxidative stress in maize (Zea mays L.) seedlings by NO and H2S donors through differential organ-dependent regulation of ROS and NADPH-recycling metabolisms.

    abstract::Chromium (Cr) contamination in soil is a growing concern in relation to sustainable agricultural production and food safety. Nitric oxide (NO) and, more recently, hydrogen sulfide (H2S) are considered to be new signalling molecules with biotechnological applications in the agronomical sector. Using 9-day-old maize (Ze...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2017.09.010

    authors: Kharbech O,Houmani H,Chaoui A,Corpas FJ

    更新日期:2017-12-01 00:00:00

  • Developing embryos of Sesbania sesban have unique potential to photosynthesize under high osmotic environment.

    abstract::This study has been carried out to investigate the photosynthetic activities in developing embryos of Sesbania sesban under a highly osmotic environment. In S. sesban, the embryo turns green/chlorophyllous at the early heart shape stage. Interestingly, despite being deeply embedded within the supporting tissues (sever...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2004.03.002

    authors: Puthur JT,Saradhi PP

    更新日期:2004-10-01 00:00:00

  • Abscisic Acid-related changes in composition and physical state of membranes in bean leaves.

    abstract::Leaf discs of bean, maize, and wheat were incubated in the presence of either 2-(14)C mevalonic acid or methyl-(14)C choline chloride and varying concentrations of abscisic acid. Abscisic acid inhibited, dependent on concentration, the incorporation of mevalonate into free sterols and stimulated the incorporation of c...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(85)80197-8

    authors: Farkas T,Singh B,Nemecz G

    更新日期:1985-03-01 00:00:00

  • Optimization of Plant Regeneration from Long Term Embryogenic Callus Cultures of Pennisetum purpureum Schum. (Napier grass).

    abstract::The effect of subculture interval and total culture age on plant regeneration from serially subcultured, leaf derived embryogenic callus cultures of Pennisetum purpureum (Napier grass) was determined. During 38 weeks of culture both embryogenic callus proliferation and the yield of plants was maximal for callus from f...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/S0176-1617(84)80028-0

    authors: Chandler SF,Vasil IK

    更新日期:1984-12-01 00:00:00

  • Polyamine anabolic/catabolic regulation along the woody grapevine plant axis.

    abstract::The distribution of the endogenous PA fractions throughout the entire perennial woody grapevine (Vitis vinifera L.) plant was studied, along with the expression profiles of the PA anabolic and catabolic genes and their substrates and secondary metabolites. Putrescine fractions increased with increasing leaf age, altho...

    journal_title:Journal of plant physiology

    pub_type: 杂志文章

    doi:10.1016/j.jplph.2009.03.013

    authors: Paschalidis KA,Moschou PN,Toumi I,Roubelakis-Angelakis KA

    更新日期:2009-09-15 00:00:00