Optically mediated nerve stimulation: Identification of injury thresholds.

Abstract:

BACKGROUND AND OBJECTIVE:Transient optical nerve stimulation is a promising new non-contact, spatially precise, artifact-free neural excitation technique useful in research and clinical settings. This study evaluates safety of this pulsed infrared laser technique by histopathologic examination of stimulated peripheral nerves. STUDY DESIGN/MATERIALS AND METHODS:Exposed rat sciatic nerves were functionally stimulated with the pulsed Holmium:YAG laser, previously validated as an effective tool for optical stimulation. Nerves were removed immediately and up to 2 weeks after stimulation and assessed histologically for thermal damage. Laser parameters studied include upper limits for radiant exposure, repetition rate, and duration of stimulation. RESULTS:Radiant exposures with <1% probability of thermal tissue damage (0.66-0.70 J/cm(2)) are significantly greater than radiant exposures required for reliable stimulation (0.34-0.48 J/cm(2)). The upper limit for safe laser stimulation repetition rate occurs near 5 Hz. Maximum duration for constant low repetition rate stimulation (2 Hz) is approximately 4 minutes with adequate tissue hydration. CONCLUSION:Results confirm that optical stimulation has the potential to become a powerful non-contact clinical and research tool for brief nerve stimulation with low risk of nerve thermal damage.

journal_name

Lasers Surg Med

authors

Wells JD,Thomsen S,Whitaker P,Jansen ED,Kao CC,Konrad PE,Mahadevan-Jansen A

doi

10.1002/lsm.20522

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

513-26

issue

6

eissn

0196-8092

issn

1096-9101

journal_volume

39

pub_type

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