Bronchoalveolar lavage quality influences the T4/T8 ratio in sarcoidosis.

Abstract:

BACKGROUND:Sarcoidosis is characterised by a T-lymphocytic alveolitis with a typically increased T4/T8 ratio. The diagnostic value of this ratio is under debate. AIM OF THE WORK:We prospectively evaluated the influence of BAL pre-lavage and the impact of bronchial contamination on BAL differential cell count in 108 BAL specimens obtained from patients with histologically confirmed sarcoidosis. METHODS:BAL was performed by instilling 150-300 ml normal saline either in the middle lobe or the lingula. Fifty-one patients (47%) underwent additional pre-lavage with 50 ml normal saline. Bronchial contamination was assessed by semi-quantitative analysis of mucus, ciliated and squamous cells in the untreated BAL recovery. RESULTS:Pre-lavage did neither influence the lavage cellularity nor extend of contamination of the BAL. Content of mucus and ciliated cells, indicating bronchial contamination, showed a high correlation (Kendal's tau=0.61). Presence of either mucus or ciliated cells in the BAL recovery was associated with a significant lower T4/T8 ratio (mucus: 4.9 vs. 8.0, p=0.009; ciliated cells: 4.1 vs. 7.4, p=0.001). Squamous cells in the BAL recovery representing oropharyngeal contamination did not significantly influence the T4/T8 ratio (7.7 vs. 5.6, p=0.10). CONCLUSION:Bronchial contamination of BAL as determined by the presence of mucus and ciliated cells in the recovery decreases the T4/T8 ratio of BAL in sarcoidosis.

journal_name

Respir Med

journal_title

Respiratory medicine

authors

Reichenberger F,Kleiber B,Baschiera B,Bubendorf L,Brutsche M,Dalquen P,Tamm M

doi

10.1016/j.rmed.2006.11.027

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

2025-30

issue

9

eissn

0954-6111

issn

1532-3064

pii

S0954-6111(07)00188-6

journal_volume

101

pub_type

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