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The real factor for polypeptide elongation in Dictyostelium cells is EF-2B, not EF-2A.

Abstract：

:Polypeptide elongation factor 2 (EF-2) plays an essential role in protein synthesis and is believed to be indispensable for cell proliferation. Recently, it has been demonstrated that there are two kinds of EF-2 (EF-2A and EF-2B with 76.6% of sequence identity at the amino acid level) in Dictyostelium discoideum. Although the knockout of EF-2A slightly impaired cytokinesis, EF-2A null cells exhibited almost normal protein synthesis and cell growth, suggesting that there is another molecule capable of compensating for EF-2 function. Since EF-2B is the most likely candidate, we examined its function using ef-2b knockdown cells prepared by the RNAi method. Our results strongly suggest that EF-2B is required for protein synthesis and cell proliferation, functioning as the real EF-2. Interestingly, the expressions of ef-2a and ef-2b mRNAs during development are reversely regulated, and the ef-2b expression is greatly augmented in ef-2a null cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Yoshino T,Maeda Y,Amagai A

doi

10.1016/j.bbrc.2007.05.155

subject

Has Abstract

pub_date

2007-08-03 00:00:00

pages

586-91

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)01113-8

journal_volume

359

pub_type

杂志文章

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