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Bis-pyrene-labeled molecular beacon: a monomer-excimer switching probe for the detection of DNA base alteration.

Abstract：

:A new bis-pyrene-labeled oligonucleotide probe (BP-probe) has been designed for the detection of a single base mismatch in single strand (ss) DNA as a target. The sequence of BP-probe was chosen to form stem-loop structure similar to a molecular beacon (MB-probe), yielding bis-pyrene-labeled molecular beacon (BP-MB-probe). Partially double stranded (ds) BP-MB-probes were prepared by complexation with oligonucleotides whose sequences are complementary to the loop segment but not to the stem and exchangeable with the target DNA. The partially ds BP-MB-probes were shown to exhibit monomer fluorescence as major fluorescence, while the ss BP-MB-probe in the stem-loop form displays strong excimer fluorescence. The strand exchange reactions between partially ds BP-MB-probe and target ss DNA in the presence of cationic comb-type copolymer as a catalyst were monitored by the excimer fluorescence changes. The existence of a mismatched base can be determined by the slower PASE rates compared with fully matched DNA.

journal_name

Bioorg Med Chem

journal_title

Bioorganic & medicinal chemistry

authors

Yamana K,Ohshita Y,Fukunaga Y,Nakamura M,Maruyama A

doi

10.1016/j.bmc.2007.04.053

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

78-83

issue

1

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(07)00381-1

journal_volume

16

pub_type

杂志文章

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