Growth and survival of Fusarium solani-F. oxysporum complex on stressed multipurpose contact lens care solution films on plastic surfaces in situ and in vitro.


PURPOSE:To analyze factors implicating the association of ReNu with MoistureLoc (ReNu ML) multipurpose contact lens solution (MPS) with the increased incidence of Fusarium keratitis. METHODS:Used contact lens cases with and without contact lenses and MPS containers were collected from patients with confirmed or possible Fusarium keratitis. Direct microscopy including transparent adhesive tape preparations and swab cultures were used to determine fungal colonization. Survival and growth of selected isolates of Fusarium spp. in drying MPS on plastic surfaces were determined by microscopy and recoverable colony counts on enriched agar. RESULTS:Discrete regions of fungal colonization, including occasional microcycle conidiation and chlamydospore formation, were observed on the surfaces of contact lens cases and, less often, on solution containers that had been used by patients with Fusarium keratitis associated with the use of ReNu ML. Isolates provisionally grouped with the F. solani-F. oxysporum complex were inhibited by fresh MPS in original solution containers and contact lens cases, but survived in stressed (drying) films of MPS, particularly ReNu ML. These in vitro test results were similar to the direct in situ observations of the materials from patients. CONCLUSIONS:Selective, rapid growth and survival of cells of the F. solani-F. oxysporum complex on plastic surfaces, particularly of contact lens cases with stressed ReNu ML films, may explain, in part, the recent Fusarium keratitis outbreak.






Zhang S,Ahearn DG,Noble-Wang JA,Stulting RD,Schwam BL,Simmons RB,Pierce GE,Crow SA Jr




Has Abstract


2006-12-01 00:00:00
















  • The Topcon SP 1000 and Image-NET systems. A comparison of four methods for evaluating corneal endothelial cell density.

    abstract::The objective of this study was to compare four methods of counting corneal endothelial cell density using the Topcon SP 1000 microscope and Image-NET digital graphic software. Two independent observers quantified cell density from 63 endothelial photomicrographs using the various standardized techniques. The first me...


    pub_type: 杂志文章


    authors: Vecchi M,Braccio L,Orsoni JG

    更新日期:1996-05-01 00:00:00

  • Recovery of Corneal Clarity After DMEK Followed by Rebubbling Versus Uneventful DMEK.

    abstract:PURPOSE:To evaluate the effect of a single rebubbling procedure after Descemet membrane endothelial keratoplasty (DMEK) on corneal backscatter and endothelial cell loss (ECL). METHODS:Medical records of 130 consecutive DMEK cases (118 patients) were reviewed and 3 groups were formed: group 1 (n = 32), consisting of DM...


    pub_type: 杂志文章


    authors: Lazaridis A,Fydanaki O,Giallouros E,Georgalas I,Kymionis G,Sekundo W,Droutsas K

    更新日期:2018-07-01 00:00:00

  • Corneal Neurotization With a Great Auricular Nerve Graft: Effective Reinnervation Demonstrated by In Vivo Confocal Microscopy.

    abstract:PURPOSE:We describe the first case of minimally invasive corneal neurotization with the great auricular nerve (GAN) to treat unilateral neurotrophic keratopathy. We assessed corneal sensation and reinnervation by esthesiometry and confocal microscopy over 12 months of follow-up, and we provide a detailed description of...


    pub_type: 杂志文章


    authors: Benkhatar H,Levy O,Goemaere I,Borderie V,Laroche L,Bouheraoua N

    更新日期:2018-05-01 00:00:00

  • Descemet-stripping automated endothelial keratoplasty by using suture for donor insertion.

    abstract:PURPOSE:To describe a new technique for donor insertion in cases of Descemet-stripping automated endothelial keratoplasty (DSAEK). METHODS:This is a retrospective case series study of a novel method to introduce the donor tissue in DSAEK. This is a report of 16 cases that underwent the DSAEK procedure. The surgical te...


    pub_type: 杂志文章


    authors: Sarnicola V,Toro P

    更新日期:2008-08-01 00:00:00

  • Dissection plane in deep anterior lamellar keratoplasty using the big-bubble technique.

    abstract:PURPOSE:To find the level of dissection in keratoconic corneas undergoing deep anterior lamellar keratoplasty (DALK) using the big-bubble technique. METHODS:In 3 keratoconic eyes that underwent DALK, the operation was converted into penetrating keratoplasty after complete big-bubble formation because of extensive tear...


    pub_type: 杂志文章


    authors: Jafarinasab MR,Rahmati-Kamel M,Kanavi MR,Feizi S

    更新日期:2010-04-01 00:00:00

  • Evaluation of Meibomian Glands in Obstructive Sleep Apnea-Hypopnea Syndrome.

    abstract:PURPOSE:To evaluate the effect of obstructive sleep apnea-hypopnea syndrome (OSAHS) on the meibomian glands, ocular surface, and tear parameters. METHODS:The study included 59 individuals (32 subjects with OSAHS, 27 control subjects) who underwent polysomnography in the Chest Diseases Clinic. The right eyes of all ind...


    pub_type: 杂志文章


    authors: Muhafiz E,Ölçen M,Erten R,Bozkurt E

    更新日期:2020-06-01 00:00:00

  • Combined use of subconjunctival and intracorneal bevacizumab injection for corneal neovascularization.

    abstract:PURPOSE:To report on the safety and clinical use of combined subconjunctival and intracorneal bevacizumab for corneal neovascularization. METHODS:The charts of 12 consecutive patients with corneal neovascularization who received combined subconjunctival and intracorneal injections of bevacizumab (2.5 mg/0.1 mL) were r...


    pub_type: 杂志文章


    authors: Yeung SN,Lichtinger A,Kim P,Amiran MD,Slomovic AR

    更新日期:2011-10-01 00:00:00

  • Immunohistochemical Study of SARS-CoV-2 Viral Entry Factors in the Cornea and Ocular Surface.

    abstract:PURPOSE:To confirm the ocular tropism of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by evaluating the expression of viral entry factors in human ocular tissues using immunohistochemistry. METHODS:Fresh donor corneas and primary explant cultures of corneal, limbal, and conjunctival epithelial cells we...


    pub_type: 杂志文章


    authors: Roehrich H,Yuan C,Hou JH

    更新日期:2020-12-01 00:00:00

  • Refractive Surgery: Malpractice Litigation Outcomes.

    abstract:PURPOSE:To review data on malpractice claims related to refractive surgery to identify common allegations and injuries and financial outcomes. METHODS:The WestlawNext database was reviewed for all malpractice lawsuits/settlements related to refractive eye surgery. Data evaluated included patient demographics, type of ...


    pub_type: 杂志文章


    authors: Custer BL,Ballard SR,Carroll RB,Barnes SD,Justin GA

    更新日期:2017-10-01 00:00:00

  • Comparison of the Center and Flex-Center Methods of Corneal Endothelial Cell Analysis in the Presence of Guttae.

    abstract:PURPOSE:To compare endothelial cell analysis by the center and flex-center methods in corneas with guttae of differing severity and to determine the minimum countable cell number for using only the flex-center method. METHODS:Forty-seven eyes with corneal guttae and 50 age-matched normal controls were enrolled in the ...


    pub_type: 杂志文章


    authors: Huang J,Liu X,Tepelus TC,Nazikyan T,Chopra V,Sadda SR,Lee OL

    更新日期:2017-12-01 00:00:00

  • Cluster of Post-Operative Endophthalmitis Caused by Acanthamoeba T10 Genotype - A First Report.

    abstract:PURPOSE:To report a cluster of postoperative Acanthamoeba endophthalmitis after routine cataract surgeries. METHODS:A brief summary of sentinel events leading to the referral of 4 patients of postoperative endophthalmitis to our hospital is followed by clinical descriptions and the various diagnostic approaches and in...


    pub_type: 杂志文章


    authors: Rammohan R,Hajib Naraharirao M,Veerappan S,Vijayaraghavan P,Rajaraman R,Manayath GJ,Dsouza P,Radhakrishnan S,Venkatapathy N,Lakshmipathi D,Madhuravasal Krishnan J,Raghavan A

    更新日期:2021-02-01 00:00:00

  • Posterior amorphous corneal dystrophy. An ultrastructural study of a variant with histopathological features of an endothelial dystrophy.

    abstract::Posterior amorphous corneal dystrophy (PACD) is a rare autosomal-dominant disease, generally classified with the pre-Descemet's dystrophies. It is characterized by deep stromal corneal opacification, flat corneas with low keratometry values, and central thinning. To our knowledge, only one previous ultrastructural stu...


    pub_type: 杂志文章


    authors: Roth SI,Mittelman D,Stock EL

    更新日期:1992-03-01 00:00:00

  • New findings for an old disease: morphological studies on pseudoexfoliation syndrome-related keratopathy and binocular asymmetry.

    abstract:PURPOSE:To review our previous studies regarding the morphology of the cornea in pseudoexfoliation syndrome (PEX) and anatomical discrepancies between PEX and contralateral eyes in unilateral PEX. METHODS:We used in vivo confocal microscopy to observe the corneas of PEX, contralateral, and normal control eyes. Morphol...


    pub_type: 杂志文章


    authors: Zheng X

    更新日期:2013-11-01 00:00:00

  • Epithelial innervation of human cornea: a three-dimensional study using confocal laser scanning fluorescence microscopy.

    abstract:PURPOSE:Evaluation of a new method to visualize distribution and morphology of human corneal nerves (Adelta- and C-fibers) by means of fluorescence staining, confocal laser scanning microscopy, and 3-dimensional (3D) reconstruction. METHODS:Trephinates of corneas with a diagnosis of Fuchs corneal dystrophy were sliced...


    pub_type: 杂志文章


    authors: Guthoff RF,Wienss H,Hahnel C,Wree A

    更新日期:2005-07-01 00:00:00

  • Quantification and Patterns of Endothelial Cell Loss Due to Eye Bank Preparation and Injector Method in Descemet Membrane Endothelial Keratoplasty Tissues.

    abstract:PURPOSE:To evaluate endothelial cell damage after eye bank preparation and passage through 1 of 2 different injectors for Descemet membrane endothelial keratoplasty grafts. METHODS:Eighteen Descemet membrane endothelial keratoplasty grafts were prepared by Lions VisionGift with the standard partial prepeel technique a...


    pub_type: 杂志文章


    authors: Schallhorn JM,Holiman JD,Stoeger CG,Chamberlain W

    更新日期:2016-03-01 00:00:00

  • Heterozygous Avellino corneal dystrophy 9 years after photorefractive keratectomy: natural or laser-induced accelerated course?

    abstract:PURPOSE:To report a photorefractive keratectomy (PRK)-treated patient who first developed stromal opacities with a typical granular pattern 9 years after surgery, which was confirmed as heterozygous Avellino dystrophy by DNA analysis. METHODS:A 37-year-old woman, who had undergone PRK 17 years ago, has been followed f...


    pub_type: 杂志文章


    authors: Park SH,Mok J,Joo CK,Kim MS

    更新日期:2009-05-01 00:00:00

  • Femtosecond laser-assisted superficial lamellar keratectomy for the treatment of superficial corneal leukomas.

    abstract:PURPOSE:To determine if superficial lamellar corneal excision with femtosecond laser followed by phototherapeutic keratectomy (PTK) using a masking substance improves visual acuity and optical quality of patients with severe corneal opacities. METHODS:Twelve eyes with corneal haze or scarring underwent confocal micros...


    pub_type: 杂志文章


    authors: Alió JL,Agdeppa MC,Uceda-Montanes A

    更新日期:2011-03-01 00:00:00

  • A safe Nd:YAG retroprosthetic membrane removal technique for keratoprosthesis.

    abstract:PURPOSE:To describe a technique for Nd:YAG laser photodiscission of retroprosthetic membrane (RPM) in keratoprosthesis patients that minimizes damage to the optic. METHODS:Prospective review of Boston type I keratoprosthesis patients at 1 site who received Nd:YAG treatment for RPM from 2005 to 2009. Outcomes were base...


    pub_type: 杂志文章


    authors: Chak G,Aquavella JV

    更新日期:2010-10-01 00:00:00

  • Anterior Segment Findings in Patients With Osteogenesis Imperfecta: A Case-Control Study.

    abstract:PURPOSE:To evaluate the anterior segment parameters in patients with osteogenesis imperfecta (OI) compared with healthy control subjects. METHODS:Seventeen patients with OI and 19 age-matched healthy controls were included into this cross-sectional case-control study. Corneal topographic, topometric and Belin-Ambrósio...


    pub_type: 杂志文章


    authors: Keleş A,Doğuizi S,Şahin NM,Koç M,Aycan Z

    更新日期:2020-08-01 00:00:00

  • Antimicrobial activity of antibiotic-treated amniotic membrane: An in vitro study.

    abstract:PURPOSE:To investigate if amniotic membrane incubated with antibiotics could inhibit bacterial growth in vitro. METHODS:Amniotic membrane fragments were incubated with the antibiotics (netilmicin) solution; the washed and drained fragments were either tested after treatment or further incubated in antibiotic-free medi...


    pub_type: 杂志文章


    authors: Mencucci R,Menchini U,Dei R

    更新日期:2006-05-01 00:00:00

  • Yin and yang in cytokine regulation of corneal wound healing: roles of TNF-alpha.

    abstract::During wound healing, corneal tissue has to restore its transparency for proper vision. Various cytokines and growth factors are believed to orchestrate cellular behavior in a healing cornea. This review summarizes the roles of 1 such factor, the proinflammatory cytokine tumor necrosis factor (TNF)-alpha, in the proce...


    pub_type: 杂志文章,评审


    authors: Saika S

    更新日期:2007-10-01 00:00:00

  • Measurement of Corneal Biomechanical Properties in Diabetes Mellitus Using the Ocular Response Analyzer and the Corvis ST.

    abstract:PURPOSE:Hyperglycemia in diabetes mellitus (DM) might induce changes in corneal biomechanics. Therefore, biomechanical properties of the cornea were measured using the ocular response analyzer and the Corvis ST. METHODS:In the study, 35 eyes of 35 diabetic patients were included. After an ophthalmological examination,...


    pub_type: 杂志文章


    authors: Ramm L,Herber R,Spoerl E,Pillunat LE,Terai N

    更新日期:2019-05-01 00:00:00

  • Multimodal Assessment of Corneal Thinning Using Optical Coherence Tomography, Scheimpflug Imaging, Pachymetry, and Slit-Lamp Examination.

    abstract:PURPOSE:To assess the relationship between corneal thinning measured by clinician-graded slit-lamp examination compared with ultrasound pachymetry (USP), anterior segment optical coherence tomography (AS-OCT), and the Pentacam. METHODS:Patients with corneal thinning underwent USP, AS-OCT, Pentacam measurements and sta...


    pub_type: 杂志文章


    authors: Oatts JT,Keenan JD,Mannis T,Lietman TM,Rose-Nussbaumer J

    更新日期:2017-04-01 00:00:00

  • Novel Technique for Descemet Membrane Remnant Stripping in Hazy Cornea During DSAEK.

    abstract:PURPOSE:To describe a novel technique for removal of Descemet membrane (DM) tags or remnants in hazy corneas during Descemet stripping automated endothelial keratoplasty. METHODS:The DM tags or remnants may be difficult to visualize in the presence of hazy corneas. To identify and remove the residual DM remnants and t...


    pub_type: 杂志文章


    authors: Sharma N,Sharma VK,Arora T,Singh KR,Agarwal T,Vajpayee RB

    更新日期:2016-01-01 00:00:00

  • Effect of intravitreal ranibizumab on corneal endothelium in age-related macular degeneration.

    abstract:PURPOSE:To determine the effect of intravitreal injection of ranibizumab on the corneal endothelium in patients with choroidal neovascularization in age-related macular degeneration. METHODS:Observational prospective case series study. Fifty-two eyes of 52 consecutive patients (29 men, 23 women; age range, 61-80 years...


    pub_type: 杂志文章


    authors: Pérez-Rico C,Benítez-Herreros J,Castro-Rebollo M,Gómez-Sangil Y,Germain F,Montes-Mollón MA,Teus MA

    更新日期:2010-08-01 00:00:00

  • Quantitation of ultraviolet light-absorbing fractions of the cornea.

    abstract::This study attempts to measure the quantitative contribution of major chemical fractions of the whole bovine cornea to ultraviolet (UV) absorption between 240 and 300 nm, with special attention to the biologically significant range of 290-300 nm. The cornea was divided into water-insoluble, nonprotein small water-solu...


    pub_type: 杂志文章


    authors: Mitchell J,Cenedella RJ

    更新日期:1995-05-01 00:00:00

  • Corneal Transplantation in Auckland, New Zealand, 1999-2009: Indications, Patient Characteristics, Ethnicity, Social Deprivation, and Access to Services.

    abstract:PURPOSE:To analyze characteristics and indications for corneal transplantation in patients undergoing penetrating, lamellar, and endothelial keratoplasty in Auckland, New Zealand (NZ). METHODS:Corneal transplantation data from the NZ National Eye Bank and hospital records of corneal transplant recipients in the Auckla...


    pub_type: 杂志文章


    authors: Crawford AZ,McKelvie J,Craig JP,McGhee CN,Patel DV

    更新日期:2017-05-01 00:00:00

  • Clinical Characteristics and Outcomes of Ocular Cicatricial Pemphigoid: A Cohort Study and Literature Review.

    abstract:PURPOSE:Ocular cicatricial pemphigoid (OCP) is a rare systemic autoimmune disease and a potentially blinding subepithelial blistering disorder. The purpose of this study was to describe the clinical spectrum of the disease and to assess the efficacy and safety of immunosuppressive agents in a cohort of patients with OC...


    pub_type: 杂志文章,评审


    authors: Fremont F,Pelissier-Suarez C,Fournié P,Porterie M,Thevenin A,Astudillo L,Paricaud K,Gualino V,Soler V,Pugnet G

    更新日期:2019-11-01 00:00:00

  • The effect of aqualase and phacoemulsification on the corneal endothelium.

    abstract:PURPOSE:To compare corneal endothelial cell loss after cataract surgery by phacoemulsification and by Aqualase. METHODS:This was a prospective, randomized study of 75 eyes of 75 patients undergoing cataract surgery. Patients were randomly assigned to receive either phacoemulsification or Aqualase for cataract removal....


    pub_type: 杂志文章,随机对照试验


    authors: Hu V,Hughes EH,Patel N,Whitefield LA

    更新日期:2010-03-01 00:00:00

  • The prevalence of positive hepatitis B, hepatitis C, and HIV serology in cornea donors prescreened by medical and social history in Ontario, Canada.

    abstract:PURPOSE:To determine the prevalence of hepatitis B (HBV), hepatitis C (HCV), and human immunodeficiency virus (HIV) in the serum of cornea donors who had been previously screened by social and medical history by the Eye Bank of Canada, Ontario Division. METHOD:A retrospective examination of the donor records of the Ey...


    pub_type: 杂志文章


    authors: Armstrong SA,Gangam N,Chipman ML,Rootman DS

    更新日期:1997-09-01 00:00:00