The oxidation of apocynin catalyzed by myeloperoxidase: proposal for NADPH oxidase inhibition.

abstract：:Properties of phosphatidylinositol kinase activities in rabbit erythrocyte membranes were studied by measuring 32P incorporation into di- and triphosphoinositide from Mg-[gamma-32P]ATP. The Km's for 32P incorporation into di- and triphosphoinositide were 110 and 48 microM ATP, respectively. The optimal temperature for...

journal_title：Archives of biochemistry and biophysics

authors： Quist EE,Barker RC

更新日期：1983-04-01 00:00:00

abstract：:Mitogens increase prostaglandin synthesis in fibroblasts. In the present studies, transcriptional activation of the prostaglandin G/H synthase isoenzyme 2 (PGHS-2) gene is shown to be responsible for this induction. Transcription of the PGHS-2 gene maximally increased within 15 min of stimulation, and elevated cellula...

journal_title：Archives of biochemistry and biophysics

authors： Evett GE,Xie W,Chipman JG,Robertson DL,Simmons DL

更新日期：1993-10-01 00:00:00

abstract：:Rats were fed toxic levels of methionine with or without simultaneous dietary supplements of glycine and serine. Feed intake, growth rate, and metabolite concentrations in intestine, plasma, liver, skeletal muscle, and kidneys were monitored. Both toxic amounts of methionine and supplemental glycine and serine affecte...

journal_title：Archives of biochemistry and biophysics

authors： Regina M,Korhonen VP,Smith TK,Alakuijala L,Eloranta TO

更新日期：1993-02-01 00:00:00

abstract：:A catalase-related allene oxide synthase (cAOS) and true catalases that metabolize hydrogen peroxide have similar structure around the heme. One of the distal heme residues considered to help control catalysis is a highly conserved asparagine. Here we addressed the role of this residue in metabolism of the natural sub...

journal_title：Archives of biochemistry and biophysics

authors： Gao B,Boeglin WE,Brash AR

更新日期：2008-09-15 00:00:00

abstract：:Horseradish peroxidase (EC 1.11.1.7) is shown to catalyze the aerobic oxidation of lysozyme, bovine serum albumin, and protamine adducts with glycolaldehyde at physiological pH. This reaction is accompanied by light emission, which is attributed to the generation of triplet species. The intensity of chemiluminescence ...

journal_title：Archives of biochemistry and biophysics

authors： de Medeiros MH,Bechara EJ

更新日期：1986-07-01 00:00:00

abstract：:Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

journal_title：Archives of biochemistry and biophysics

authors： Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

更新日期：1998-06-15 00:00:00

abstract：:2-Isopropenyl-2-methyladamantane (2-PMADA) and 3-isopropenyl-3-methyldiamantane (3-PMDIA) showed potent and selective inhibition of cytochrome P450 (CYP) 2B6-mediated reactions with K(i) values of 5.27 and 2.17 microM, respectively. No effect on activities of other human CYP was found even at concentrations 100-fold h...

journal_title：Archives of biochemistry and biophysics

authors： Stiborová M,Borek-Dohalská L,Hodek P,Mráz J,Frei E

更新日期：2002-07-01 00:00:00

abstract：:Cultured arterial smooth muscle cells have been found to contain an activatable neutral cholesteryl esterase (EC 3.1.1.13). This enzyme is similar to that previously described in adipose tissue, adrenal cortex, and aortic homogenates. Although both the lysosomal (acid) and cytoplasmic (neutral) cholesteryl esterases w...

journal_title：Archives of biochemistry and biophysics

authors： Hajjar DP

更新日期：1986-05-15 00:00:00

abstract：:Substrate specificity of human mitochondrial low Km aldehyde dehydrogenase (EC 1.2.1.3) E2 isozyme has been investigated employing p-nitrophenyl esters of acyl groups of two to six carbon atoms and comparing with that of aldehydes of one to eight carbon atoms. The esterase reaction was studied under three conditions: ...

journal_title：Archives of biochemistry and biophysics

authors： Mukerjee N,Pietruszko R

更新日期：1992-11-15 00:00:00

abstract：:Previously, we reported that several charged amino acids located in the alpha1-beta4 loop of the iron-sulfur protein are required to maintain the stability of the protein and its assembly into the cytochrome bc1 complex. The current study extends this analysis to several amino acids localized in the single alpha-helix...

journal_title：Archives of biochemistry and biophysics

authors： Obungu VH,Beattie DS

更新日期：1998-11-01 00:00:00

abstract：:The Ku autoantigen is a heterodimeric protein of 70- and 83-kDa subunits, endowed with duplex DNA end-binding capacity and DNA helicase activity (Human DNA Helicase II, HDH II). HDH II/Ku is well established as the DNA binding component, the regulatory subunit as well as a substrate for the DNA-dependent protein kinas...

journal_title：Archives of biochemistry and biophysics

authors： Ochem AE,Rechreche H,Skopac D,Falaschi A

更新日期：2008-02-01 00:00:00

abstract：:In contrast to other P450 enzymes purified from rat liver microsomes, purified P450 IIIA1 (P450p) is catalytically inactive when reconstituted with NADPH-cytochrome P450 reductase and the synthetic lipid, dilauroylphosphatidylcholine. However, purified P450 IIIA1 catalyzes the oxidation of testosterone when reconstitu...

journal_title：Archives of biochemistry and biophysics

authors： Eberhart DC,Parkinson A

更新日期：1991-12-01 00:00:00

abstract：:Three major pathways mediate amino acid transport into mammalian cells: the A-system and the ASC-system, which require a sodium gradient across the plasma membrane, and the L-system, which has no requirement for a sodium gradient. We have found that the lymphocytes from patients with B-cell chronic lymphocytic leukemi...

journal_title：Archives of biochemistry and biophysics

authors： Segel GB,Tometsko AM,Lichtman MA

更新日期：1985-11-01 00:00:00

abstract：:B4-2-1 cells (Lec15 cells) are Chinese hamster ovary cells deficient in mannosylphosphoryldolichol synthase activity. They synthesize the truncated lipid intermediate Man5GlcNAc2-P-P-dolichol rather than the Glc3Man9GlcNAc2-P-P-dolichol synthesized by wild-type cells. In this report we present evidence that these cell...

journal_title：Archives of biochemistry and biophysics

authors： Stoll J,Cacan R,Verbert A,Krag SS

更新日期：1992-12-01 00:00:00

abstract：:Histidinol dehydrogenase (EC 1.1.1.23) activity was determined in several plant species and in cultured plant cell lines. The enzyme was purified from cabbage (Brassica oleracea) to apparent homogeneity. To render complete purification, a new, specific histidinol-Sepharose 4B affinity chromatography was developed. The...

journal_title：Archives of biochemistry and biophysics

authors： Nagai A,Scheidegger A

更新日期：1991-01-01 00:00:00

abstract：:Uptake of L-[3H]glutamate by monolayers of fibroblasts cultured from human embryonic skin has been studied in the presence of several nonradioactive structural analogs of glutamate and aspartate. Results have suggested that the structural specificites of glutamate transporters in cultured human fibroblasts are similar...

journal_title：Archives of biochemistry and biophysics

authors： Cooper B,Chebib M,Shen J,King NJ,Darvey IG,Kuchel PW,Rothstein JD,Balcar VJ

更新日期：1998-05-15 00:00:00

abstract：:Previous studies demonstrated that liver microsomes from untreated rats catalyze the omega, omega-1, and omega-2 hydroxylation of prostaglandins [K. A. Holm, R. J. Engell, and D. Kupfer (1985) Arch. Biochem. Biophys. 237, 477-489]. The current study examined the regioselectivity of hydroxylation of PGE1 and PGE2 by pu...

journal_title：Archives of biochemistry and biophysics

authors： Kupfer D,Jansson I,Favreau LV,Theoharides AD,Schenkman JB

更新日期：1988-02-15 00:00:00

abstract：:Chlorophyllase catalyzes the initial step in the degradation of chlorophyll and plays a key role in leaf senescence and fruit ripening. Here, we report the cloning of chlorophyllase from Triticum aestivum (wheat) and provide a detailed mechanistic analysis of the enzyme. Purification of recombinant chlorophyllase from...

journal_title：Archives of biochemistry and biophysics

authors： Arkus KA,Cahoon EB,Jez JM

更新日期：2005-06-15 00:00:00

abstract：:A glucosyltransferase, which catalyzed the transfer of glucose from UDP-glucose (UDPG) to p-hydroxybenzoate (PHB) in cell cultures of Lithospermum erythrorhizon Sieb. et Zucc., Boraginaceae, was purified 219-fold by ammonium sulfate fractionation and chromatography on DEAE-Sephacel, Sephadex G-150, and phenyl-Sepharos...

journal_title：Archives of biochemistry and biophysics

authors： Bechthold A,Berger U,Heide L

更新日期：1991-07-01 00:00:00

abstract：:A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane prote...

journal_title：Archives of biochemistry and biophysics

authors： Perryman RA,Mooney B,Harmey MA

更新日期：1995-02-01 00:00:00

abstract：:Site-directed mutagenesis was used to probe the role of glycine residue 336 in the regulatory properties of Escherichia coli ADP-glucose pyrophosphorylase. This residue was previously found to be changed from glycine to aspartate in the gene of an Escherichia coli mutant strain. The mutant enzyme had altered kinetic p...

journal_title：Archives of biochemistry and biophysics

authors： Meyer CR,Bork JA,Nadler S,Yirsa J,Preiss J

更新日期：1998-05-01 00:00:00

abstract：:Peptides are not only useful models for the structural understanding of protein folding and stability but also provide promising therapeutic avenues for the treatment of numerous diseases, and as biomaterials. The field has been very active over the last decades, but the complex conformational behaviour of peptides st...

journal_title：Archives of biochemistry and biophysics

authors： Morales P,Jiménez MA

更新日期：2019-01-01 00:00:00

abstract：:Sequence alignment of pig mitochondrial NADP-dependent isocitrate dehydrogenase with eukaryotic (human, rat, and yeast) and Escherichia coli isocitrate dehydrogenases reveals that Tyr316 is completely conserved and is equivalent to the E. coli Tyr345, which interacts with the 2'-phosphate of NADP in the crystal struct...

journal_title：Archives of biochemistry and biophysics

authors： Lee P,Colman RF

更新日期：2002-05-01 00:00:00

abstract：:Isolated adrenocortical carcinoma cells of rat contain alpha 2- and beta-adrenergic receptors. When these cells are incubated with alpha 2-adrenergic agonists, there is a concentration-dependent increase of cyclic GMP that is blocked by the alpha 2-adrenergic antagonist yohimbine but not by the beta-antagonist propran...

journal_title：Archives of biochemistry and biophysics

authors： Jaiswal N,Sharma RK

更新日期：1986-09-01 00:00:00

abstract：:Bovine heart muscle microsomes rapidly convert lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) in the presence of oleoyl-CoA. Both substrates are incorporated into the product, although the rate of incorporation of radiolabel into PC from 1-[14C]palmitoyl-LPC was approximately threefold higher than the rat...

journal_title：Archives of biochemistry and biophysics

authors： Sanjanwala M,Sun GY,Cutrera MA,MacQuarrie RA

更新日期：1988-09-01 00:00:00

abstract：:The treatment of male rats with Hg2+ resulted in significant alterations in heme and hemoprotein metabolism in the adrenal gland which, in turn, were reflected in abnormal steroidogenic activities and steroid output. Twenty-four hours after the administration of 30 mumol of HgCl2/kg (sc) the mitochondrial heme and cyt...

journal_title：Archives of biochemistry and biophysics

authors： Veltman JC,Maines MD

更新日期：1986-08-01 00:00:00

abstract：:Human tumor necrosis factor (TNF), first isolated by our group as an anticancer agent, has been now shown to be a primary mediator of inflammation. Till today 19 different members of the TNF superfamily which interact with 29 different receptors, have been identified. Most members of this family exhibit pro-inflammato...

journal_title：Archives of biochemistry and biophysics

authors： Gupta SC,Tyagi AK,Deshmukh-Taskar P,Hinojosa M,Prasad S,Aggarwal BB

更新日期：2014-10-01 00:00:00

abstract：:Chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. Chlorite dismutase-like proteins are incapable of performing this reaction and their biologic...

journal_title：Archives of biochemistry and biophysics

authors： Hofbauer S,Hagmüller A,Schaffner I,Mlynek G,Krutzler M,Stadlmayr G,Pirker KF,Obinger C,Daims H,Djinović-Carugo K,Furtmüller PG

更新日期：2015-05-15 00:00:00

abstract：:Mutations in MYBPC3, the gene encoding cardiac myosin binding protein C (cMyBP-C), are a major cause of hypertrophic cardiomyopathy (HCM). While most mutations encode premature stop codons, missense mutations causing single amino acid substitutions are also common. Here we investigated effects of a single proline for ...

journal_title：Archives of biochemistry and biophysics

authors： van Dijk SJ,Bezold Kooiker K,Mazzalupo S,Yang Y,Kostyukova AS,Mustacich DJ,Hoye ER,Stern JA,Kittleson MD,Harris SP

更新日期：2016-07-01 00:00:00

abstract：:G protein-coupled receptors can be directly modulated by changes in transmembrane voltage in a variety of cell types. Here we show that, while changes in the membrane voltage itself do not induce detectable modifications in the cytosolic Ca(2+) concentration, platelet stimulation with thrombin or the PAR-1 and PAR-4 a...

journal_title：Archives of biochemistry and biophysics

authors： Albarrán L,Dionisio N,López E,Salido GM,Rosado JA

更新日期：2013-10-15 00:00:00