Stimulation of the DNA unwinding activity of human DNA helicase II/Ku by phosphorylation.

abstract：:We previously showed that iron chelated to the Pseudomonas aeruginosa siderophore pyochelin enhances oxidant-mediated injury to pulmonary artery endothelial cells by catalyzing hydroxyl radical (HO(*)) formation. Therefore, we examined pyochelin structural/chemical features that may be important in this process. Five ...

journal_title：Archives of biochemistry and biophysics

authors： DeWitte JJ,Cox CD,Rasmussen GT,Britigan BE

更新日期：2001-09-15 00:00:00

abstract：:Calmodulin is phosphorylated by the purified insulin receptor on tyrosine residues with a maximum stoichiometry of 1 mol phosphate/mol of calmodulin. Isolated tryptic phosphopeptides were sequenced by manual Edman degradation and demonstrated that calmodulin is equally phosphorylated on tyrosine 99 and tyrosine 138. P...

journal_title：Archives of biochemistry and biophysics

authors： Williams JP,Jo H,Sacks DB,Crimmins DL,Thoma RS,Hunnicutt RE,Radding W,Sharma RK,McDonald JM

更新日期：1994-11-15 00:00:00

abstract：:A human, 85-kDa arachidonoyl-specific, cytosolic phospholipase A2 was expressed using the baculovirus-insect cell expression system. Expression resulted in the production of an active protein which consisted of approximately 3% of the total protein in the host Spodoptera frugiperda (Sf9) cells at 67 h after infection....

journal_title：Archives of biochemistry and biophysics

authors： de Carvalho MS,McCormack FX,Leslie CC

更新日期：1993-11-01 00:00:00

abstract：:An N-acetyl-D-galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide alpha-D-GalNAc-(1,3)-[alpha-L-Fuc-(1,2)]-beta-D-Gal (Synsorb A). The affinity-purified lectin appears to b...

journal_title：Archives of biochemistry and biophysics

authors： Maliarik MJ,Roberts DD,Goldstein IJ

更新日期：1987-05-15 00:00:00

abstract：:The steady-state kinetic mechanism of vitamin K-dependent carboxylase from calf liver has been investigated by initial-velocity measurements with varying concentrations of two carboxylase substrates and constant, nonsaturating concentrations of the other two substrates. With all combinations of the varied substrates t...

journal_title：Archives of biochemistry and biophysics

authors： Uotila L

更新日期：1988-07-01 00:00:00

abstract：:Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

journal_title：Archives of biochemistry and biophysics

authors： Korotchkina LG,Ciszak EM,Patel MS

更新日期：2004-09-15 00:00:00

abstract：:Alcohol oxidase (AO) is a peroxisomal, homo-octameric flavoenzyme, which catalyzes methanol oxidation in methylotrophic yeast. Here, we report on the generation of soluble, FAD-lacking AO monomers. Using steady-state fluorescence, fluorescence correlation spectroscopy, circular dichroism and static light scattering ap...

journal_title：Archives of biochemistry and biophysics

authors： Visser NV,Wang D,Stanley WA,Groves MR,Wilmanns M,Veenhuis M,van der Klei IJ

更新日期：2007-03-15 00:00:00

abstract：:Nitroxides stable radicals are unreactive toward most diamagnetic molecules, but readily undergo one-electron redox reactions with paramagnetic species such as free radicals and transition metals, thus serving as cell-permeable antioxidants. The cytotoxicity of juglone (5-hydroxy-1,4-naphthoquinone), like that of othe...

journal_title：Archives of biochemistry and biophysics

authors： Zhang R,Hirsch O,Mohsen M,Samuni A

更新日期：1994-08-01 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:The antioxidant, butylated hydroxytoluene (BHT), causes lung toxicity in mice followed by regenerative repair, and can also modulate the development of carcinogen-induced lung adenomas. We are investigating changes in pulmonary biochemistry following BHT treatment in order to understand the mechanisms of BHT-induced p...

journal_title：Archives of biochemistry and biophysics

authors： Blumenthal EJ,Malkinson AM

更新日期：1987-07-01 00:00:00

abstract：:We investigated the role of free radicals in hemoglobin (Hb) oxidation and denaturation. To generate free radicals, we used two azocompounds, the hydrophilic 2,2'-azobis(2-amidinopropane hydrochloride and the hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) and a drug of the quinone family, phenazine methosulfate. T...

journal_title：Archives of biochemistry and biophysics

authors： Minetti M,Mallozzi C,Scorza G,Scott MD,Kuypers FA,Lubin BH

更新日期：1993-04-01 00:00:00

abstract：:Uteroglobin has been purified from hare lung by gel filtration and chromatography on carboxymethyl-cellulose. Hare uteroglobin appears homogeneous by electrophoresis under both denaturing and nondenaturing conditions. Its chemical and immunological properties as well as its ability to bind progesterone are compared to...

journal_title：Archives of biochemistry and biophysics

authors： Soledad López de Haro M,Nieto A

更新日期：1983-10-15 00:00:00

abstract：:Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： LaMarco KL,Glew RH

更新日期：1985-02-01 00:00:00

abstract：:The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

journal_title：Archives of biochemistry and biophysics

authors： Holden HM,Rayment I

更新日期：1991-11-15 00:00:00

abstract：:We evaluated the expression and activity of rat 12-lipoxygenase (LO) in rat intestinal epithelial (RIE) cells during apoptosis and cell differentiation. Sodium butyrate (NaBT) treatment induced wild-type RIE (W-RIE) cells to undergo differentiation and apoptosis. Alkaline phosphatase (ALP) activity, a marker of cell d...

journal_title：Archives of biochemistry and biophysics

authors： Kamitani H,Ikawa H,Hsi LC,Watanabe T,DuBois RN,Eling TE

更新日期：1999-08-01 00:00:00

abstract：:Differential scanning calorimetry measures the heat capacity of states and the excess heat associated with transitions that can be induced by temperature change. The integral of the excess heat capacity is the enthalpy for this process. Despite this potentially intimidating sounding physical chemistry background, DSC ...

journal_title：Archives of biochemistry and biophysics

authors： Johnson CM

更新日期：2013-03-01 00:00:00

abstract：:As part of an attempt to understand the specific function and role of each subunit in multisubunit protein synthesis factors, we have attempted to identify the nucleotide binding peptides of eukaryotic initiation factor 2 (eIF-2). To ensure that the interactions were of a specific nature, two general controls were use...

journal_title：Archives of biochemistry and biophysics

authors： Anthony DD Jr,Kinzy TG,Merrick WC

更新日期：1990-08-15 00:00:00

abstract：:Caspase-12, mainly detected in endoplasmic reticulum (ER), has been suggested to play a role in ER-mediated apoptosis and inflammatory caspase activation pathway. Cleavage of the prodomain by caspase-3/-7 at the carboxyl terminus of Asp94 or m-calpain at the carboxyl terminus of Lys158 was reported to be a part of cas...

journal_title：Archives of biochemistry and biophysics

authors： Lee HJ,Lee SH,Park SH,Sharoar MG,Shin SY,Lee JS,Cho B,Park IS

更新日期：2010-10-01 00:00:00

abstract：:Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI-/+ mating pairs were used to generate global KO offspring. Of 323 ...

journal_title：Archives of biochemistry and biophysics

authors： Avery JC,Yamazaki Y,Hoffmann FW,Folgelgren B,Hoffmann PR

更新日期：2020-08-15 00:00:00

abstract：:The taxa-4(20),11(12)-dien-5alpha-ol-O-acetyl transferase which catalyzes the third step of Taxol biosynthesis has been isolated from methyl jasmonate-induced Taxus cells, and partially purified and characterized (K. Walker, R. E. B. Ketchum, M. Hezari, D. Gatfield, M. Golenowski, A. Barthol, and R. Croteau, Arch. Bio...

journal_title：Archives of biochemistry and biophysics

authors： Walker K,Schoendorf A,Croteau R

更新日期：2000-02-15 00:00:00

abstract：:Utilizing RNA blot hybridization and immunoblotting techniques, the changes of the hepatic contents of acetyl-CoA carboxylase mRNA and of the enzyme protein in growing chicks have been investigated. In the post-hatching period, the hepatic mRNA level markedly increased at least 70-fold when compared to that before hat...

journal_title：Archives of biochemistry and biophysics

authors： Takai T,Saito Y,Yamamoto K,Tanabe T

更新日期：1988-11-01 00:00:00

abstract：:2,2'-Anhydro-3'-deoxy-5-ethyluridine, a new pyrimidine nucleoside analog, has been examined in terms of its binding potency to uridine phosphorylase, and its conformation in solution (NMR) was studied. 2,2'-Anhydro-3'-deoxy-5-ethyluridine has a Ki value of 3.4 microM for uridine phosphorylase from rat intestinal mucos...

journal_title：Archives of biochemistry and biophysics

authors： Veres Z,Neszmélyi A,Szabolcs A,Kiss AI,Dénes G

更新日期：1991-04-01 00:00:00

abstract：:Calcium-induced changes in protein solubility play a role in a variety of important biological processes including the deposition of bone and dentin and the secretion of milk. The phenomena of salt-induced (calcium) precipitation of proteins (salting-out), and the resolubilization of these proteins at higher salt conc...

journal_title：Archives of biochemistry and biophysics

authors： Farrell HM Jr,Kumosinski TF,Pulaski P,Thompson MP

更新日期：1988-08-15 00:00:00

abstract：:Linamarase (EC 3.2.1.21) was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

journal_title：Archives of biochemistry and biophysics

authors： Eksittikul T,Chulavatnatol M

更新日期：1988-10-01 00:00:00

abstract：:Catalases are biotechnologically relevant enzymes because of their applications in food technology, bioremediation, and biomedicine. The dismutation of hydrogen peroxide occurs in two steps; in the first one, the enzyme forms an oxidized compound I (Cpd I) and in the second one, the enzyme is reduced to the ferric sta...

journal_title：Archives of biochemistry and biophysics

authors： Zárate-Romero A,Stojanoff V,Cohen AE,Hansberg W,Rudiño-Piñera E

更新日期：2019-05-15 00:00:00

abstract：:Speed of processing is a particularly important characteristic of the visual system. Often a behavioral reaction to a visual stimulus must be faster than the conscious perception of that stimulus, as is the case with many sports (e.g., baseball). Visual psychophysics provides a relatively simple and precise means of m...

journal_title：Archives of biochemistry and biophysics

authors： Bovier ER,Hammond BR

更新日期：2015-04-15 00:00:00

abstract：:The native conformation of bovine retinal arrestin has been characterized by a variety of spectroscopic methods. The purified protein gives rise to a near uv absorption band centered at 279 nm which results from the absorbance of its 14 tyrosine and one tryptophan residue. The extinction coefficient for this absorptio...

journal_title：Archives of biochemistry and biophysics

authors： Kotake S,Hey P,Mirmira RG,Copeland RA

更新日期：1991-02-15 00:00:00

abstract：:Arachidonate 12-lipoxygenase (12-LO) from porcine leukocytes was expressed in insect cells using a baculovirus expression vector. The recombinant 12-LO was expressed as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site. Maximal intracellular en...

journal_title：Archives of biochemistry and biophysics

authors： Reddy RG,Yoshimoto T,Yamamoto S,Funk CD,Marnett LJ

更新日期：1994-07-01 00:00:00

abstract：:The intracellular location of pyruvate carboxylase (EC 6.4.1.1) in rat liver and Saccharomyces cerevisiae was investigated using the antibody-gold and protein A-gold techniques carried out as a postembedding immunoelectron microscopic procedure. The vast majority of gold particles (greater than 98%), indicative of the...

journal_title：Archives of biochemistry and biophysics

authors： Rohde M,Lim F,Wallace JC

更新日期：1991-10-01 00:00:00

abstract：:The relative rates of transcription of the rat liver glutathione S-transferase Ya-Yc and Yb genes were determined in purified liver nuclei isolated at different times after phenobarbital or 3-methylcholanthrene administration. The transcriptional rates of the Ya-Yc and Yb genes were elevated approximately fivefold 8 a...

journal_title：Archives of biochemistry and biophysics

authors： Ding VD,Pickett CB

更新日期：1985-08-01 00:00:00