Abstract:
BACKGROUND AND OBJECTIVE:The goal of this study was to use an inexpensive macroscopic imaging system to monitor tumor progression in mouse models in real-time with minimal intervention. STUDY DESIGN/MATERIALS AND METHODS:Illumination is provided via a xenon arc lamp and a fiber optic probe which delivers white light or quasi-monochromatic excitation via specific bandpass filters. Fluorescence emission from SCID and nude mice following mammary fat pad injection of red fluorescence protein (RFP)-expressing human breast cancer cell lines was recorded and quantified using a single lens reflex (SLR) digital camera. RESULTS:This simple system enabled the verification of successful tumor take and temporal quantification of tumor progression in mouse models. CONCLUSION:The macroscopic fluorescence imaging system represents an inexpensive and portable tool to facilitate non-invasive in situ cancer detection with the potential to monitor fluorescent tumor formation and investigation of the efficacy of potential cancer therapeutics.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Carlson AL,Hoffmeyer MR,Wall KM,Baugher PJ,Richards-Kortum R,Dharmawardhane SFdoi
10.1002/lsm.20409subject
Has Abstractpub_date
2006-12-01 00:00:00pages
928-38issue
10eissn
0196-8092issn
1096-9101journal_volume
38pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:The aim was to investigate the bactericidal effect of the 1,064 nm Nd:YAG laser on Staphylococcus epidermidis. MATERIAL AND METHODS:S. epidermidis was inoculated on agar plates and then exposed to pulsed laser light in three different modes: with an uninterrupted train of pulses, or with two d...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)27:4<336::aid-lsm6>3.0.co;2
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Fiber-assisted laser surgery has been employed as a minimally invasive method in various medical fields. In spite of multiple sweeping on tissue during laser treatments, the rate of tissue removal gradually decreases and eventually leads to longer irradiation times as well as deeper thermal inj...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22505
更新日期:2016-08-01 00:00:00
abstract::The use of pulsed laser radiation to recanalize occluded biliary endoprostheses was examined. In vitro measurements demonstrated that a repetitively pulsed organic dye laser transmitted through a small diameter quartz fiber can recanalize an occluded stent in less than 10 sec without causing visible damage to the sten...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070503
更新日期:1987-01-01 00:00:00
abstract::A laser endoscope was devised using optic fibers in order to provide a means of visualization and removal of atheromatous plaques from arteries in cadavers and experimental animals. Atheromatous plaques were satisfactorily lased off in a few seconds, although contraction of lased vessels was observed. A self-focusing ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900040408
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Light dosimetry for endobronchial photodynamic therapy is not very advanced to date. This study investigates the dependency of the fluence rate distribution in the bronchial wall on several parameters. STUDY DESIGN/MATERIALS AND METHODS:A Monte Carlo model is employed for the illumination of a...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)22:4<193::aid-lsm3>3.
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Nonmelanoma skin cancers, including basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), are the most common skin cancers, presenting nearly as many cases as all other cancers combined. The current gold-standard for clinical diagnosis of these lesions is histopathologic examination, a...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20653
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of the in vitro study was to examine the clinical efficacy of semiconductor laser periodontal pocket irradiation as an adjunct to conventional scaling and root planing. MATERIALS AND METHODS:Twenty-two healthy patients with a need of periodontal treatment (15 women, 7 men, mean age 45...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20252
更新日期:2005-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Chlorophyllin-M is a new chlorophyll-based derivative photosensitive compound developed by our research group with easy laboratorial synthesis and ideal properties for photodynamic therapy (PDT). It is intended for clinical treatments with simple and low cost techniques and reagents. The object...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22362
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Little information is available regarding the effectiveness of laser prostatectomy in patients with urinary retention from benign prostatic hyperplasia since there is no paper specifically dealing with laser prostatectomy in patients in urinary retention. STUDY DESIGN/MATERIALS AND METHODS:Twe...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)19:4<480::AID-LSM15>3
更新日期:1996-01-01 00:00:00
abstract::A 1-J/cm2 dose of single laser-irradiation applied on primary human embryo fibroblast culture was not followed either by functional or micromorphological alterations of cell surfaces. This dose, however, applied four times with 24-hour intervals changed the functional conditions as well as surface charges of cell memb...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900040411
更新日期:1984-01-01 00:00:00
abstract::The author's experience in the use of the argon laser in the treatment of superficial vascular lesions in children is reviewed. Deteriorating portwine stains, strawberry hemangiomas, other hemangiomas, telangiectasias, and other lesions are discussed. Their natural histories are discussed. Details are given regarding ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060106
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Objectively measure and compare postoperative collagen thermal damage and subsequent new collagen synthesis after cutaneous laser resurfacing using two carbon dioxide laser systems. STUDY DESIGN/MATERIALS AND METHODS:We created 240 resurfacing wounds on eight piglets with scanned and short-pul...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)23:2<66::aid-lsm3>3.0
更新日期:1998-01-01 00:00:00
abstract::The feasibility of using a pulsed dye laser in angioplasty for detection and disintegration of calcified plaques was studied in vitro. A flashlamp-pumped dye laser (495 nm, 2 microseconds) was used as the exciting source for laser-induced-fluorescence (LIF) signals. Spectral data in the 520 nm to 800 nm region of norm...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110207
更新日期:1991-01-01 00:00:00
abstract::A synthetic contact sapphire endoprobe attached to the quartz fiber of a neodymium:yttrium aluminum garnet (Nd:YAG) laser had been used in the transurethral treatment of bladder tumors. A total of 68 operations have been carried out in 48 patients. The contact probe was placed directly into the tumor under direct visi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070616
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The relationship between tissue ablation volume and the formation of insoluble gas of the currently available excimer laser systems is unknown. This aspect was evaluated in two excimer laser systems. STUDY DESIGN/MATERIALS AND METHODS:We measured tissue ablation volume and gas production of tw...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:2<197::AID-LSM11>3
更新日期:1996-01-01 00:00:00
abstract::Fluorescence spectroscopy is a promising new technique for discrimination of normal and atherosclerotic arterial tissues. It has been suggested that this technique be used as a guidance system for laser angiosurgery catheters; however, irradiation by 476-nm light can change the spectroscopic properties of arterial tis...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090606
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Holmium laser lithotripsy is the gold standard for intracorporeal fragmentation of urinary calculi. Usually, a visible beam is superimposed on the IR treatment laser as an aiming beam to guide the surgeon. In vitro tests showed that this aiming beam (532 nm, power <1 mW) excites strong fluores...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22611
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Short pulsed and scanned CO(2) lasers that target water molecules are currently used for cutaneous resurfacing. These CO(2) resurfacing lasers produce acute cutaneous contraction, which can be quantitated as a measure of the laser's effect. We postulated that targeting the vibrational and rotat...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1999)25:1<1::aid-lsm1>3.0.
更新日期:1999-01-01 00:00:00
abstract::Photodynamic therapy (PDT) is a promising approach to the treatment of cancer. Preferential retention of the photosensitizer by malignant tissue has been considered a hallmark of this treatment modality. However, photosensitivity can be observed in normal, non-neoplastic tissues, and the present study investigated the...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110110
更新日期:1991-01-01 00:00:00
abstract::The use of lasers as surgical tools may open up new possibilities for the treatment of atherosclerotic occlusive vascular disease. However, basic information regarding the effect of laser radiation to the lumen of normal blood vessels is needed prior to clinical application. This work investigates the nature of the CO...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900030308
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The sanitation of the root canal system and the adjacent dentin has always been a key requirement for successful endodontics. In recent years, various laser systems have provided a major contribution to this aim, namely the Nd:YAG-, the 810 nm Diode-, the Er:YAG-, and the Er,Cr:YSGG laser. Num...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20331
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To demonstrate that high color fidelity light-emitting diode (LED) sources are preferred by dermatologists for the evaluation of patients during standard-of-care, outpatient visits when compared to low color fidelity LED sources similar to fluorescent lighting. STUDY DESIGN/MATERIALS AND METH...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23352
更新日期:2020-11-08 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Infrared neural stimulation (INS) has recently evoked interest as an alternative to electrical stimulation. The mechanism of activation is the heating of water, which induces changes in cell membrane potential but may also trigger heat sensitive receptors. To further elucidate the mechanism, wh...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22158
更新日期:2013-09-01 00:00:00
abstract::Seven cases of cerebral tumors (four deep-seated sovratentorial gliomas located in motor area, occipital region, parietal region and frontal region; one deep-seated cystic cerebellar spongioblastoma; one sphenoidal wing meningioma, and one spinal cord intradural tumor) were simultaneously irradiated in the same area w...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900020205
更新日期:1982-01-01 00:00:00
abstract::We used a Q-switched Nd:YAG laser with non-linear optical crystals to produce the 5th (213 nm) and the 4th (266 nm) harmonic frequencies. Using these two wavelengths, we ablated fresh porcine corneas and type I collagen synthetic epikeratoplasty lenticules. For the 213-nm ablation, radiant exposure was 1.3 J/cm2. The ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110610
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:An ideal treatment for pilonidal disease should feature a low recurrence rate, minimal incision, and rapid return to daily activities. We compared the outcomes of the well-defined Karydakis technique (KT) and a combination of pit excision (PE) and sinus tract ablation using 1,470-nm diode lase...
journal_title:Lasers in surgery and medicine
pub_type:
doi:10.1002/lsm.23224
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Platelet-rich plasma (PRP) which contains large amounts of growth factors has been tried to enhance therapeutic efficacy of laser treatment for acne scar with unknown underlying mechanism. OBJECTIVES:The present study was conducted to investigate the molecular mechanism of increased clinical efficacy of PRP...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22776
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Acne scarring is a common complication of acne but no effective single treatment modality has been developed. To compare the efficacy of 1,550 nm Er:Glass fractional laser and chemical reconstruction of skin scar (CROSS) method in the treatment of acne scars. STUDY DESIGN/MATERIALS AND METHODS...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.20796
更新日期:2009-10-01 00:00:00
abstract::The transport of biologically active material during laser cutting with CO2 and Er lasers is demonstrated. This transport mechanism removes particles from the surface of gelatin, agar, and liver samples into the depth of the laser-formed craters. The transport phenomenon is explained by a contraction and condensation ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900080604
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Currently there is no safe, effective, and rapid means to eliminate the pain associated with a needle insertion through the skin. It is hypothesized that ablation of the stratum corneum layer using a low energy Erbium(Er):YAG laser would allow rapid local anesthesia from a lidocaine product. ...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.10101
更新日期:2002-01-01 00:00:00