In situ analysis of breast cancer progression in murine models using a macroscopic fluorescence imaging system.

Abstract:

BACKGROUND AND OBJECTIVE:The goal of this study was to use an inexpensive macroscopic imaging system to monitor tumor progression in mouse models in real-time with minimal intervention. STUDY DESIGN/MATERIALS AND METHODS:Illumination is provided via a xenon arc lamp and a fiber optic probe which delivers white light or quasi-monochromatic excitation via specific bandpass filters. Fluorescence emission from SCID and nude mice following mammary fat pad injection of red fluorescence protein (RFP)-expressing human breast cancer cell lines was recorded and quantified using a single lens reflex (SLR) digital camera. RESULTS:This simple system enabled the verification of successful tumor take and temporal quantification of tumor progression in mouse models. CONCLUSION:The macroscopic fluorescence imaging system represents an inexpensive and portable tool to facilitate non-invasive in situ cancer detection with the potential to monitor fluorescent tumor formation and investigation of the efficacy of potential cancer therapeutics.

journal_name

Lasers Surg Med

authors

Carlson AL,Hoffmeyer MR,Wall KM,Baugher PJ,Richards-Kortum R,Dharmawardhane SF

doi

10.1002/lsm.20409

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

928-38

issue

10

eissn

0196-8092

issn

1096-9101

journal_volume

38

pub_type

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