Abstract：
:The EEN (extra eleven nineteen) gene, located on chromosome 19p13, was cloned as a fusion with MLL from a patient with acute myeloid leukemia (AML) with translocation t(11;19)(q23;p13). In this study, we characterized the genomic structure of the EEN gene, including its 5' regulatory region and transcription start site (TSS). We found that Sp1 could bind to the guanine-cytosine (GC)-stretch of the EEN promoter and was critical for the normal EEN expression, whereas the leukemia-associated fusion protein AML1-ETO could aberrantly transactivate the EEN gene through an AML1 binding site. Of note, overexpressed EEN showed oncogenic properties, such as transforming potential in NIH3T3 cells, stimulating cell proliferation, and increasing the activity of transcriptional factor AP-1. Retroviral transduction of EEN increased self-renewal and proliferation of murine hematopoietic progenitor cells. Moreover, Kasumi-1 and HL60-cell growth was inhibited with down-regulation of EEN by RNAi. These findings demonstrate that EEN might be a common target in 2 major types of AML associated with MLL or AML1 translocations, and overexpression of EEN may play an essential role in leukemogenesis.
journal_name
Bloodjournal_title
Bloodauthors
Ma LH,Liu H,Xiong H,Chen B,Zhang XW,Wang YY,Le HY,Huang QH,Zhang QH,Li BL,Chen Z,Chen SJdoi
10.1182/blood-2006-02-003517subject
Has Abstractpub_date
2007-01-15 00:00:00pages
769-77issue
2eissn
0006-4971issn
1528-0020pii
blood-2006-02-003517journal_volume
109pub_type
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