Abstract:
BACKGROUND:Hyperlipidemia associated with the protease inhibitor (PI) component of highly active antiretrovial treatment can lead to accelerated atherosclerosis. The apolipoprotein A-V (APOA5) gene, which affects VLDL production and lipolysis, may play a role in PI-induced hyperlipidemia, particularly in individuals with the APOA5-1131T-->C genotype. METHODS:We measured lipoprotein changes in HIV-positive patients (n = 229) who had been followed for 5 years. For statistical analyses, we segregated the patients with respect to PI treatment and APOA5-1131T-->C genotype. RESULTS:The frequency of the C allele was 0.08, similar to that in the general population. We found a strong effect of the APOA5-1131T-->C genotype among patients receiving PIs. Carriers of the C allele had consistently increased mean (SD) triglyceride concentrations compared with noncarriers after 1 year [2.11 (1.62) vs 3.71 (4.27) mmol/L; P = 0.009], 2 years [2.48 (2.09) vs 4.02 (4.05) mmol/L, P = 0.050], 3 years [2.32 (1.71) vs 4.13 (4.26) mmol/L; P = 0.013], 4 years [2.90 (2.95) vs 5.35 (7.12) mmol/L; P was not significant], and 5 years [4.25 (5.58) vs 9.23 (9.63) mmol/L; P was not significant]. We observed the same effect on total cholesterol concentrations: after 1 year [4.93 (1.31) vs 5.87 (1.66) mmol/L; P = 0.006], 2 years [5.03 (1.12) vs 6.42 (2.48) mmol/L; P = 0.001], 3 years [5.11 (1.17) vs 6.38 (2.43) mmol/L; P = 0.009], 4 years [5.49 (1.71) vs 6.78 (3.03) mmol/L; P was not significant], and 5 years [5.56 (1.75) vs 7.90 (3.60) mmol/L; P was not significant]. HDL cholesterol showed a progressive reduction, leading to a considerably higher cholesterol/HDL cholesterol ratio after 3 years. CONCLUSION:Variability in the APOA5 gene predisposes patients with HIV, particularly those treated with PI, to severe hyperlipidemia.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Guardiola M,Ferré R,Salazar J,Alonso-Villaverde C,Coll B,Parra S,Masana L,Ribalta Jdoi
10.1373/clinchem.2006.069583subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1914-9issue
10eissn
0009-9147issn
1530-8561pii
clinchem.2006.069583journal_volume
52pub_type
杂志文章abstract::We describe the performance of an RIA for the measurement of prostate-specific antigen (PA). Between-assay precision (CV) for control sera with various analyte concentrations was as follows: mean = 1.67 micrograms/L, CV = 7.1%; mean = 4.47 micrograms/L, CV = 5.6%; mean = 7.15 micrograms/L, CV = 5.5% (n = 19 each). Ana...
journal_title:Clinical chemistry
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doi:
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abstract:BACKGROUND:We evaluated assays to measure both total tissue plasminogen activator (tPA) and the three principle forms of tPA in plasma: active tPA, tPA complexed with plasminogen activator inhibitor type 1 (PAI-1), and tPA complexed with C1-inhibitor. METHODS:Active tPA was measured by use of an indirect amidolytic as...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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abstract:BACKGROUND:Mitochondrial diseases are clinically and genetically heterogeneous, with variable penetrance, expressivity, and differing age of onset. Disease-causing point mutations and large deletions in the mitochondrial genome often exist in a heteroplasmic state. Current molecular analyses require multiple different ...
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pub_type: 杂志文章
doi:10.1373/clinchem.2011.181438
更新日期:2012-09-01 00:00:00
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更新日期:1986-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1975-09-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:2003-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1975-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1997-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1979-08-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2008-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1980-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-07-01 00:00:00
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doi:
更新日期:1998-08-01 00:00:00
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doi:
更新日期:1995-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1979-10-01 00:00:00
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doi:
更新日期:1993-03-01 00:00:00
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pub_type: 临床试验,杂志文章
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journal_title:Clinical chemistry
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doi:
更新日期:1978-10-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1976-10-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:2014-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1998-01-01 00:00:00
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更新日期:1994-10-01 00:00:00
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更新日期:2011-01-01 00:00:00
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