Origin and ontogeny of mammalian ovarian neurons.


:Mammalian ovaries contain sympathetic neurons expressing the low affinity neurotropin receptor (p75NTR). To date neither the role these neurons might play in ovarian physiology nor their embryological origin is known. Immunohistochemistry was used to detect postnatal changes in distribution and number of both p75NTR-positive and tyrosine hydroxylase-positive neurons in rhesus monkey ovaries. Pig fetuses were used to map the pathway of ovarian neuronal migration during embryonic development. Antiserum to p75NTR revealed the presence of isolated neurons and neurons clustered into ganglia in 2-month-old monkey ovaries. After 8 months, the neurons exhibited well-developed processes, and other than being more extensively interlaced, the localization and morphology did not change after 2 yr of age. Total number of p75NTR-positive neurons present decreased gradually between 2 months and 12 yr of age and declined markedly with reproductive aging. Conversely, the subpopulation of neurons immunoreactive to anti-tyrosine hydroxylase increased significantly at puberty and then declined with the loss of reproductive capacity. By d 21 of fetal life in the pig, p75NTR neurons had migrated medially from the neural crest to form the paraaortic autonomic ganglia. Some neurons migrated ventrally from the ganglia and then continued ventrolaterally to enter the genital ridge. By d 27, neurons had entered the developing ovary, and by d 35, the migration was complete with neurons demonstrating immunoreactivity to NeuN, a neuron-specific marker. Results demonstrate that p75NTR-expressing ovarian neurons originate from the neural crest and that a catecholaminergic subset is associated with pubertal maturation of the ovary and subsequent reproductive function.






Dees WL,Hiney JK,McArthur NH,Johnson GA,Dissen GA,Ojeda SR




Has Abstract


2006-08-01 00:00:00














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    pub_type: 杂志文章


    authors: Patel O,Dumesny C,Shulkes A,Baldwin GS

    更新日期:2007-03-01 00:00:00

  • Transcriptional and metabolic effects of glucocorticoid receptor α and β signaling in zebrafish.

    abstract::In humans and zebrafish, 2 glucocorticoid (GC) receptor (GR) splice variants exist: the canonical GR α-isoform (GRα), and the GRβ. In the present study, we have used the zebrafish model system in order to reveal genes affected by each of these 2 receptor isoforms. By injecting zebrafish embryos with different splice-b...


    pub_type: 杂志文章


    authors: Chatzopoulou A,Roy U,Meijer AH,Alia A,Spaink HP,Schaaf MJ

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  • Contribution of growth hormone to the adipogenic activity of serum.

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    pub_type: 杂志文章


    authors: Nixon T,Green H

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  • A role for the endogenous opioid beta-endorphin in energy homeostasis.

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    pub_type: 杂志文章


    authors: Appleyard SM,Hayward M,Young JI,Butler AA,Cone RD,Rubinstein M,Low MJ

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  • Effects of knockout of the protein kinase C beta gene on glucose transport and glucose homeostasis.

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    pub_type: 杂志文章


    authors: Standaert ML,Bandyopadhyay G,Galloway L,Soto J,Ono Y,Kikkawa U,Farese RV,Leitges M

    更新日期:1999-10-01 00:00:00

  • Lactogenesis in explant cultures of mammary tissue from pregnant cows.

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    pub_type: 杂志文章


    authors: Collier RJ,Bauman DE,Hays RL

    更新日期:1977-04-01 00:00:00

  • Localization of olfactory cyclic nucleotide-gated channels in rat gonadotropin-releasing hormone neurons.

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    pub_type: 杂志文章


    authors: El-Majdoubi M,Weiner RI

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  • Follicle-stimulating hormone-regulated Sertoli cell proteins SCc1 and SCc2 are phosphorylated and mitochondrially associated.

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    pub_type: 杂志文章


    authors: Gregory CW,DePhilip RM

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  • Peptidylarginine deiminase in rat pituitary: sex difference, estrous cycle-related changes, and estrogen dependence.

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    pub_type: 杂志文章


    authors: Senshu T,Akiyama K,Nagata S,Watanabe K,Hikichi K

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  • Neonatal dexamethasone administration. II. Persistent alteration of circadian serum anterior pituitary hormone rhythms in rats.

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    pub_type: 杂志文章


    authors: Poland RE,Rubin RT,Weichsel ME Jr

    更新日期:1981-03-01 00:00:00

  • Biosynthesis and posttranslational processing of the neurotensin/neuromedin N precursor in the rat medullary thyroid carcinoma 6-23 cell line. Effect of dexamethasone.

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    pub_type: 杂志文章


    authors: de Nadai F,Rovere C,Bidard JN,Laur J,Martinez J,Cuber JC,Kitabgi P

    更新日期:1993-04-01 00:00:00

  • Selective deficiency of guanine nucleotide-binding protein Go in two dopamine-resistant pituitary tumors.

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    authors: Collu R,Bouvier C,Lagacé G,Unson CG,Milligan G,Goldsmith P,Spiegel AM

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  • Leptin increases the viability of isolated rat pancreatic islets by suppressing apoptosis.

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    pub_type: 杂志文章


    authors: Okuya S,Tanabe K,Tanizawa Y,Oka Y

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    pub_type: 杂志文章


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  • Involvement of vasopressin in the down-regulation of pituitary corticotropin-releasing factor receptors after adrenalectomy.

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    pub_type: 杂志文章


    authors: Holmes MC,Catt KJ,Aguilera G

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  • Rab8B GTPase and junction dynamics in the testis.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Lim GE,Huang GJ,Flora N,LeRoith D,Rhodes CJ,Brubaker PL

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    pub_type: 杂志文章


    authors: Hayes MR,Skibicka KP,Grill HJ

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    pub_type: 杂志文章


    authors: Sapino A,Macrì L,Tonda L,Bussolati G

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  • Autoimmune oophoritis with multiple molecular targets mitigated by transgenic expression of mater.

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    pub_type: 杂志文章


    authors: Otsuka N,Tong ZB,Vanevski K,Tu W,Cheng MH,Nelson LM

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    pub_type: 杂志文章


    authors: Lu M,Miller C,Habener JF

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    pub_type: 杂志文章


    authors: Smith SG 3rd,Matt KS,Prestowitz WF,Stetson MH

    更新日期:1982-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Kemp SF,Hintz RL

    更新日期:1980-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Toaff ME,Schleyer H,Strauss JF 3rd

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    pub_type: 杂志文章


    authors: Keembiyehetty CN,Candelaria RP,Majumdar G,Raghow R,Martinez-Hernandez A,Solomon SS

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    pub_type: 杂志文章


    authors: Galbiati M,Zanisi M,Messi E,Cavarretta I,Martini L,Melcangi RC

    更新日期:1996-12-01 00:00:00

  • Modulation of Dickkopf-1 attenuates glucocorticoid induction of osteoblast apoptosis, adipocytic differentiation, and bone mass loss.

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    pub_type: 杂志文章


    authors: Wang FS,Ko JY,Yeh DW,Ke HC,Wu HL

    更新日期:2008-04-01 00:00:00

  • The aryl hydrocarbon receptor pathway and sexual differentiation of neuroendocrine functions.

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    pub_type: 杂志文章,评审


    authors: Petersen SL,Krishnan S,Hudgens ED

    更新日期:2006-06-01 00:00:00