Abstract:
:This study demonstrates an innovative application of atomic force microscopy (AFM). The combination of high-resolution AFM technology and tape stripping is presented as a tool for the structure analysis of human stratum corneum (SC) at a nanometer scale. Topographic images with a vertical resolution of about 10 nm of the SC are presented. Topographical and structural differences between aged and young skin can be observed. Aged skin SC is characterized by an increased single-cell surface area, prominent intercellular gaps and enhanced cell surface roughness. The use of AFM in combination with other already established methods, e.g. tape stripping in the field of dermatological research will give new insights to the structure, function and morphodynamics of SC.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Gorzelanny C,Goerge T,Schnaeker EM,Thomas K,Luger TA,Schneider SWdoi
10.1111/j.0906-6705.2006.00424.xsubject
Has Abstractpub_date
2006-05-01 00:00:00pages
387-91issue
5eissn
0906-6705issn
1600-0625pii
EXD424journal_volume
15pub_type
杂志文章abstract::In the area of dermatological research, the knowledge of rate and extent of substance penetration into the human skin is essential not only for evaluation of therapeutics, but also for risk assessment of chemicals and cosmetic ingredients. Recently, confocal Raman microscopy emerged as a novel analytical technique for...
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