Abstract:
:We studied the physiological basis of local adaptation to drought in Boechera holboellii, a perennial relative of Arabidopsis thaliana, and used cDNA-AFLPs to identify candidate genes showing differential expression in these populations. We compared two populations of B. holboellii from contrasting water environments in a reciprocal transplant experiment, as well as in a laboratory dry-down experiment. We continuously measured the water content of soils using time domain reflectometery (TDR). We compared populations for their water use efficiency (WUE), root/shoot ratios (R:S) and leaf mass per unit area (LMA) in the field and in the laboratory, and identified candidate genes that (i) responded plastically to water stress and (ii) were differentially expressed between the two populations. Genotypes from the drier site had higher WUE, which was attributable to a large reduction in transpirational water loss. The xeric-adapted population also had increased investment in root biomass and greater leaf mass per unit area. Reciprocal transplants in the field had significantly greater survival in their native habitat. In total, 450 cDNA-AFLP fragments showed significant changes between drought and control treatments. Furthermore, some genes showed genotype (population)-specific patterns of up- or down-regulation in response to drought. Three hundred cDNA-AFLP bands were sequenced leading to the identification of cDNAs coding for proteins involved in signal transduction, transcriptional regulation, redox regulation, oxidative stress and pathways involved in stress adaptation. Some of these proteins could contribute a physiological advantage under drought, making them potential targets for natural selection.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Knight CA,Vogel H,Kroymann J,Shumate A,Witsenboer H,Mitchell-Olds Tdoi
10.1111/j.1365-294X.2006.02818.xsubject
Has Abstractpub_date
2006-04-01 00:00:00pages
1229-37issue
5eissn
0962-1083issn
1365-294Xpii
MEC2818journal_volume
15pub_type
杂志文章abstract::Coral reefs in the Tropical Eastern Pacific (TEP) are among the most isolated in the world. This isolation has resulted in relatively low species diversity but comparatively high endemism. The dominant reef-building corals of the TEP are the Pocillopora corals, a ubiquitous Indo-Pacific genus commonly regarded as infe...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03672.x
更新日期:2008-03-01 00:00:00
abstract::The temperature stress that pelagic fishes experience can induce physiological and behavioural changes that leave a signature in gene expression profiles. We used a functional genomics approach to identify genes that were up- or down-regulated following thermal stress in the Pacific bluefin tuna. Following the acclima...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04174.x
更新日期:2009-05-01 00:00:00
abstract::DNA-based sex tests now exist for many avian species. However, none of these tests are widely applicable to ratites. We present DNA sequence data for a locus that is W chromosome-linked in the kiwi, ostrich, cassowary, rhea, and emu. At the amino acid level, this sequence has significant homology to X-linked genes in ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2002.01483.x
更新日期:2002-04-01 00:00:00
abstract::Because the vast majority of species are well diverged, relatively little is known about the genomic architecture of speciation during the early stages of divergence. Species within recent evolutionary radiations are often minimally diverged from a genomic perspective, and therefore provide rare opportunities to addre...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12926
更新日期:2014-11-01 00:00:00
abstract::Recently evolved species typically share genetic variation across their genomes due to incomplete lineage sorting and/or ongoing gene flow. Given only subtle allele frequency differences at most loci and the expectation that divergent selection may affect only a tiny fraction of the genome, distinguishing closely rela...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15091
更新日期:2019-05-01 00:00:00
abstract::Understanding the factors that maintain genetic variation in natural populations is a foundational goal of evolutionary biology. To this end, population geneticists have developed a variety of models that can produce stable polymorphisms. In one of the earliest models, Owen () demonstrated that differences in selectio...
journal_title:Molecular ecology
pub_type: 评论,新闻
doi:10.1111/mec.14787
更新日期:2018-09-01 00:00:00
abstract::We reanalysed published data to evaluate whether climate and habitat are barriers to dispersal in one of the most mobile and widely distributed mammals, the grey wolf (Canis lupus). Distance-based redundancy analysis (dbRDA) was used to examine the amount of variation in genetic distances that could be explained by an...
journal_title:Molecular ecology
pub_type: 杂志文章,meta分析
doi:10.1111/j.1365-294X.2004.02244.x
更新日期:2004-08-01 00:00:00
abstract::Abstract Microsatellites are powerful molecular markers, used commonly to estimate intraspecific genetic distances. With the exception of band sharing similarity index, available distance measures were developed specifically for diploid organisms and are unsuited for comparisons of polyploids. Here, we present a simpl...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2004.02209.x
更新日期:2004-07-01 00:00:00
abstract::Adaptive radiations provide unique opportunities to test whether and how recent ecological and evolutionary diversification of host species structures the composition of entire bacterial communities. We used 16S rRNA gene sequencing of faecal samples to test for differences in the gut microbiota of six species of Puer...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13796
更新日期:2016-10-01 00:00:00
abstract::Functionally constrained genes are ideal insecticide targets because disruption is often fatal, and resistance mutations are typically costly. Synaptic acetylcholinesterase (AChE) is an essential neurotransmission enzyme targeted by insecticides used increasingly in malaria control. In Anopheles and Culex mosquitoes, ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13197
更新日期:2015-06-01 00:00:00
abstract::Hybrid zones are important windows into ecological and evolutionary processes. Our understanding of the significance and prevalence of hybridization in nature has expanded with the generation and analysis of genome-spanning data sets. That said, most hybridization research still has restricted temporal and spatial res...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15514
更新日期:2020-08-01 00:00:00
abstract::Geographical isolation, habitat variation and trophic specialization have contributed to a large extent to the astonishing diversity of cichlid fishes in the Great East African lakes. Because parasite communities often vary across space and environments, parasites can accompany and potentially enhance cichlid species ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13833
更新日期:2016-11-01 00:00:00
abstract::We estimated the rate of extra-pair fertilizations (EPFs) in waved albatrosses (Phoebastria irrorata) on Isla Española, Galápagos, Ecuador, using multilocus minisatellite DNA fingerprinting. Waved albatrosses are socially monogamous, long-lived seabirds whose main population is on Española. Aggressive extra-pair copul...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2000.00996.x
更新日期:2000-09-01 00:00:00
abstract::Detecting signatures of selection in tree populations threatened by climate change is currently a major research priority. Here, we investigated the signature of local adaptation over a short spatial scale using 96 European beech (Fagus sylvatica L.) individuals originating from two pairs of populations on the norther...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12902
更新日期:2014-10-01 00:00:00
abstract::The rate of information collection generated by metagenomics is uncoupled with its meaningful ecological interpretation. New analytical approaches based on functional trait-based ecology may help to bridge this gap and extend the trait approach to the community level in vast and complex environmental genetic data sets...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05383.x
更新日期:2012-04-01 00:00:00
abstract::In High Arctic ecosystems, plant growth and reproduction are limited by low soil moisture and nutrient availability, low soil and air temperatures, and a short growing season. Mycorrhizal associations facilitate plant nutrient acquisition and water uptake and may therefore be particularly ecologically important in nut...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12646
更新日期:2014-02-01 00:00:00
abstract::The concept of kinship permeates many domains of fundamental and applied biology ranging from social evolution to conservation science to quantitative and human genetics. Until recently, pedigrees were the gold standard to infer kinship, but the advent of next-generation sequencing and the availability of dense geneti...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14833
更新日期:2018-10-01 00:00:00
abstract::Insect guts are often colonized by multispecies microbial communities that play integral roles in nutrition, digestion and defence. Community composition can differ across host species with increasing dietary and genetic divergence, yet gut microbiota can also vary between conspecific hosts and across an individual's ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12607
更新日期:2014-03-01 00:00:00
abstract::Wolbachia is a common heritable bacterial symbiont in insects. Its evolutionary success lies in the diverse phenotypic effects it has on its hosts coupled to its propensity to move between host species over evolutionary timescales. In a survey of natural host-symbiont associations in a range of Drosophila species, we ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14164
更新日期:2017-08-01 00:00:00
abstract::Experimental evolution (EE) combined with whole-genome sequencing (WGS) has become a compelling approach to study the fundamental mechanisms and processes that drive evolution. Most EE-WGS studies published to date have used microbes, owing to their ease of propagation and manipulation in the laboratory and relatively...
journal_title:Molecular ecology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-294X.2012.05484.x
更新日期:2012-05-01 00:00:00
abstract::Mitochondrial DNA sequence data were obtained for eight species of flightless Galapaganus endemic weevils and one winged close relative in order to study their colonization history and modes of diversification in the Galápagos Archipelago. Contrary to most other insular radiations, the phylogeny estimates we recovered...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03642.x
更新日期:2008-02-01 00:00:00
abstract::Determining the structure of bacterial communities and their response to stimuli is key to understanding community function and the interactions that occur between micro-organisms and the environment. However, bacterial communities often comprise complex assemblages of large numbers of different bacterial populations....
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1995.tb00263.x
更新日期:1995-10-01 00:00:00
abstract::DNA barcoding facilitates many evolutionary and ecological studies, including the examination of the dietary diversity of herbivores. In this study, we present a survey of ecological associations between herbivorous beetles and host plants from seriously threatened European steppic grasslands. We determined host plant...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13278
更新日期:2015-08-01 00:00:00
abstract::Cichlid fish are well known for their high speciation rates, which are usually accompanied by spectacular and rapid diversification in eco-morphological and secondary sexual traits. This is best illustrated by the famous repeated explosive radiations in the African Great Lakes Tanganyika, Malawi and Victoria, each lak...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/mec.13598
更新日期:2016-04-01 00:00:00
abstract::Many species of malaria and related haemosporidian parasites (Haemosporida) are responsible for diseases in wild and domestic animals. These pathogens are exclusively transmitted by blood-sucking dipteran insects (Diptera). Traditional vector studies, which are based mainly on experimental infection and subsequent dis...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/j.1365-294x.2011.05187.x
更新日期:2011-08-01 00:00:00
abstract::The cellular stress response has long been the primary model for studying the molecular basis of thermal adaptation, yet the link between gene expression, RNA metabolism and physiological responses to thermal stress remains largely unexplored. We address this by comparing the transcriptional and physiological response...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13000
更新日期:2014-12-01 00:00:00
abstract::Understanding the relative role of different evolutionary forces in shaping the level and distribution of functional genetic diversity among natural populations is a key issue in evolutionary and conservation biology. To do so accurately genetic data must be analysed in conjunction with an unambiguous understanding of...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13437
更新日期:2015-12-01 00:00:00
abstract::The inevitable has happened: genomic technologies have been added to our noninvasive genetic sampling repertoire. In this issue of Molecular Ecology, Perry et al. (2010) demonstrate how DNA extraction from chimpanzee faeces, followed by a series of steps to enrich for target loci, can be coupled with next-generation s...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/j.1365-294X.2010.04889.x
更新日期:2010-12-01 00:00:00
abstract::Island formation is a key driver of biological evolution, and several studies have used geological ages of islands to calibrate rates of DNA change. However, many islands are home to "relict" lineages whose divergence apparently pre-dates island age. The geologically dynamic New Zealand (NZ) archipelago sits upon the ...
journal_title:Molecular ecology
pub_type: 杂志文章,评审
doi:10.1111/mec.15229
更新日期:2019-10-01 00:00:00
abstract::Host specialization is a key process in ecological divergence and speciation of plant-associated fungi. The underlying determinants of host specialization are generally poorly understood, especially in endophytes, which constitute one of the most abundant components of the plant microbiome. We addressed the genetic ba...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14585
更新日期:2018-08-01 00:00:00