Abstract:
:Functionally constrained genes are ideal insecticide targets because disruption is often fatal, and resistance mutations are typically costly. Synaptic acetylcholinesterase (AChE) is an essential neurotransmission enzyme targeted by insecticides used increasingly in malaria control. In Anopheles and Culex mosquitoes, a glycine-serine substitution at codon 119 of the Ace-1 gene confers both resistance and fitness costs, especially for 119S/S homozygotes. G119S in Anopheles gambiae from Accra (Ghana) is strongly associated with resistance, and, despite expectations of cost, resistant 119S alleles are increasing significantly in frequency. Sequencing of Accra females detected only a single Ace-1 119S haplotype, whereas 119G diversity was high overall but very low at non-synonymous sites, evidence of strong purifying selection driven by functional constraint. Flanking microsatellites showed reduced diversity, elevated linkage disequilibrium and high differentiation of 119S, relative to 119G homozygotes across up to two megabases of the genome. Yet these signals of selection were inconsistent and sometimes weak tens of kilobases from Ace-1. This unexpected finding is attributable to apparently ubiquitous amplification of 119S alleles as part of a large copy number variant (CNV) far exceeding the size of the Ace-1 gene, whereas 119G alleles were unduplicated. Ace-1 CNV was detectable in archived samples collected when the 119S allele was rare in Ghana. Multicopy amplification of resistant alleles has not been observed previously and is likely to underpin the recent increase in 119S frequency. The large CNV compromised localization of the strong selective sweep around Ace-1, emphasizing the need to integrate CNV analysis into genome scans for selection.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Weetman D,Mitchell SN,Wilding CS,Birks DP,Yawson AE,Essandoh J,Mawejje HD,Djogbenou LS,Steen K,Rippon EJ,Clarkson CS,Field SG,Rigden DJ,Donnelly MJdoi
10.1111/mec.13197subject
Has Abstractpub_date
2015-06-01 00:00:00pages
2656-72issue
11eissn
0962-1083issn
1365-294Xjournal_volume
24pub_type
杂志文章abstract::Although the importance of host plant chemistry in plant-insect interactions is widely accepted, the genetic basis of adaptation to host plants is not well understood. Here, we investigate transcriptional changes associated with a host plant shift in Drosophila mettleri. While D. mettleri is distributed mainly through...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13388
更新日期:2015-10-01 00:00:00
abstract::Many genes increase coding capacity by alternate exon usage. The gene encoding the insect nicotinic acetylcholine receptor (nAChR) α6 subunit, target of the bio-insecticide spinosad, is one example of this and expands protein diversity via alternative splicing of mutually exclusive exons. Here, we show that spinosad r...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13882
更新日期:2016-11-01 00:00:00
abstract::Wolbachia is a common heritable bacterial symbiont in insects. Its evolutionary success lies in the diverse phenotypic effects it has on its hosts coupled to its propensity to move between host species over evolutionary timescales. In a survey of natural host-symbiont associations in a range of Drosophila species, we ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14164
更新日期:2017-08-01 00:00:00
abstract::Many ectothermic species are currently expanding their distributions polewards due to anthropogenic global warming. Molecular genetic mechanisms facilitating range expansion under these conditions are largely unknown, but understanding these could help mitigate expanding pests and disease vectors, or help explain why ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13548
更新日期:2016-03-01 00:00:00
abstract::Temporal changes at 16 allozyme loci in the Diplodus sargus population of Banyuls-sur-Mer (Mediterranean Sea, France) were monitored. Temporal genetic variation within a single population was examined over two temporal scales: (i) among three year-classes sampled at the same age, and (ii) within a single year-class sa...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2002.01521.x
更新日期:2002-08-01 00:00:00
abstract::As climate changes, sea surface temperature anomalies that negatively impact coral reef organisms continue to increase in frequency and intensity. Yet, despite widespread coral mortality, genetic diversity remains high even in those coral species listed as threatened. While this is good news in many ways, it presents ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14517
更新日期:2018-03-01 00:00:00
abstract::We examined male reproductive success in a common brushtail possum population in New Zealand farmland. Paternity was assigned to 66 of 91 pouch young (maternity known), using a likelihood approach applied to genotypes at six microsatellite loci having an overall average exclusion probability of around 99%. The distrib...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2000.00941.x
更新日期:2000-07-01 00:00:00
abstract::The number of exotic plant species that have been introduced into the United States far exceeds that of other groups of organisms, and many of these have become invasive. As in many regions of the globe, invasive members of the thistle tribe, Cardueae, are highly problematic in the California Floristic Province, an es...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13227
更新日期:2015-06-01 00:00:00
abstract::Individual-based assignment tests are now standard tools in molecular ecology and have several applications, including the study of dispersal. The measurement of natal dispersal is vital to understanding the ecology of many species, yet the accuracy of assignment tests in situations where natal dispersal is common rem...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2004.2081.x
更新日期:2004-03-01 00:00:00
abstract::The population dynamics of a parasite depend on species traits, host dynamics and the environment. Those dynamics are reflected in the genetic structure of the population. Habitat fragmentation has a greater impact on parasites than on their hosts because resource distribution is increasingly fragmented for species at...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13696
更新日期:2016-07-01 00:00:00
abstract::A half century ago the State of Hawaii began a remarkable, if unintentional, experiment on the population genetics of introduced species, by releasing 2431 Bluestriped Snappers (Lutjanus kasmira) from the Marquesas Islands in 1958 and 728 conspecifics from the Society Islands in 1961. By 1992 L. kasmira had spread acr...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04535.x
更新日期:2010-03-01 00:00:00
abstract::The macrogeographic population structure of the agricultural pest Bactrocera cucurbitae (Diptera: Tephritidae) was investigated in order to identify the geographic origin of the species and reconstruct its range expansion. Individuals of B. cucurbitae were collected from 25 worldwide-distributed localities (n = 570) a...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04662.x
更新日期:2010-07-01 00:00:00
abstract::For most species in the Western Palaearctic region, southern Mediterranean peninsulas have been identified as major Quaternary refugia and hotspots of intraspecific diversity, and thus, as areas of particular relevance for the conservation of the evolutionary potential. We analysed the patterns of geographical variati...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2008.03870.x
更新日期:2008-09-01 00:00:00
abstract::In the absence of pedigree information, the determination of genetic distinctness of populations can only be made by genetic methods. Using DNA fingerprinting on the North American captive herd of Speke's gazelle Gazella spekei, we were able to address two hypotheses. First, two new individuals were found to have come...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1994.tb00075.x
更新日期:1994-08-01 00:00:00
abstract::In certain cases, a species may have access to important genetic variation present in a related species via adaptive introgression. These novel alleles may interact with their new genetic background, resulting in unexpected phenotypes. In this study, we describe a selective sweep on standing variation on the X chromos...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13382
更新日期:2015-10-01 00:00:00
abstract::The Neolithic transition has been widely debated particularly regarding the extent to which this revolution implied a demographic expansion from the Near East. We attempted to shed some light on this process in northeastern Iberia by combining ancient DNA (aDNA) data from Early Neolithic settlers and published DNA dat...
journal_title:Molecular ecology
pub_type: 历史文章,杂志文章
doi:10.1111/j.1365-294X.2011.05361.x
更新日期:2012-01-01 00:00:00
abstract::Studying the evolutionary history of trait divergence, in particular those related to dispersal capacity, is of major interest for the process of local adaptation and metapopulation dynamics. Here, we reconstruct the evolution of different alleles at the nuclear-encoded mitochondrial NADP(+)-dependent isocitrate dehyd...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13031
更新日期:2015-02-01 00:00:00
abstract::An electrophoretic survey of 81 populations of arctic Daphnia pulex from around the Svalbard archipelago revealed the presence of 49 unique allozyme clones (N = 3357). Two closely related clones accounted for 66% of the total sample, and were widespread across the archipelago. Restriction fragment length polymorphisms...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1994.tb00128.x
更新日期:1994-10-01 00:00:00
abstract::The ecological and genetic factors determining the extent of introgression between species in secondary contact zones remain poorly understood. Here, we investigate the relative importance of isolating barriers and the demographic expansion of invasive Mytilus galloprovincialis on the magnitude and the direction of in...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13340
更新日期:2015-09-01 00:00:00
abstract::What is the relationship between genetic or environmental variation and the variation in messenger RNA (mRNA) expression? To address this, microarrays were used to examine the effect of genetic and environmental variation on cardiac mRNA expression for metabolic genes in three groups of Fundulus heteroclitus: (i) indi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04235.x
更新日期:2009-07-01 00:00:00
abstract::House mice are extra-ordinary animals -extra-ordinary in the literal sense of that word. They are pests - but also a valued laboratory animal. They are generalized rodents - and successful in habitats from tundra to tropics and from sea-level to high altitudes. They have differentiated into a perplexity of taxa, yet d...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/j.1365-294X.2009.04345.x
更新日期:2009-11-01 00:00:00
abstract::Recent theory has examined the way in which vaccination strategies are expected to influence the evolution of parasite virulence. Most of this work has assumed that vaccination is imposed on a homogeneous host population. However, host populations are typically composed of different types of individuals, with each typ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03418.x
更新日期:2008-01-01 00:00:00
abstract::The observation that phenotypic convergence and genetic convergence are widespread in nature implies that evolution is at least somewhat predictable. But to what extent and under what circumstances? In other words, how predictable is evolutionary predictability? Answering this question requires going beyond documentin...
journal_title:Molecular ecology
pub_type: 评论,新闻
doi:10.1111/mec.14716
更新日期:2018-06-01 00:00:00
abstract::Mate-choice theory predicts different optimal mating systems depending on resource availability and habitat stability. Regions with limited resources are thought to promote monogamy. We tested predictions of monogamy in a social rodent, the hoary marmot (Marmota caligata), at the northern climatic extreme of its distr...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.03211.x
更新日期:2007-03-01 00:00:00
abstract::Dispersal drives diverse processes from population persistence to community dynamics. However, the amount of temporal variation in dispersal and its consequences for metapopulation dynamics is largely unknown for organisms with environmentally driven dispersal (e.g., many marine larvae, arthropods and plant seeds). He...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15732
更新日期:2020-11-16 00:00:00
abstract::Inferences regarding hybridization rely on genetic markers to differentiate parental taxa from one another. Intersimple sequence repeat (ISSR) markers are based on single-primer PCR reactions where the primer sequence is derived from di- and trinucleotide repeats. These markers have successfully been used to assay gen...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1998.00425.x
更新日期:1998-09-01 00:00:00
abstract::Landscape genetics is an important framework for investigating the influence of spatial pattern on ecological process. Nevertheless, the standard analytic frameworks in landscape genetics have difficulty evaluating hypotheses about spatial processes in dynamic landscapes. We use a predictive hypothesis-driven approach...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04226.x
更新日期:2009-07-01 00:00:00
abstract::The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. B. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. This phloem-feeder harbours the primary symbiont Portiera aleyrodidarum and pot...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05087.x
更新日期:2011-05-01 00:00:00
abstract::The variation and taxonomic diversity among mammalian gut microbiomes raises several questions about the factors that contribute to the rates and patterns of change in these microbial communities. By comparing the microbiome compositions of 112 species representing 14 mammalian orders, we assessed how host and ecologi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14473
更新日期:2018-04-01 00:00:00
abstract::To understand the success of invasive species, it is important to know whether colonization events are facilitated by adaptive evolution or are limited to sites where a species is pre-adapted to thrive. Studies of the ancient colonization patterns of an invader in its native range provide an opportunity to examine its...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03508.x
更新日期:2007-10-01 00:00:00