Abstract：
:The cellular stress response has long been the primary model for studying the molecular basis of thermal adaptation, yet the link between gene expression, RNA metabolism and physiological responses to thermal stress remains largely unexplored. We address this by comparing the transcriptional and physiological responses of three geographically distinct populations of Drosophila melanogaster from eastern Australia in response to, and recovery from, a severe heat stress with and without a prestress hardening treatment. We focus on starvin (stv), recently identified as an important thermally responsive gene. Intriguingly, stv encodes seven transcripts from alternative transcription sites and alternative splicing, yet appears to be rapidly heat inducible. First, we show genetic differences in upper thermal limits of the populations tested. We then demonstrate that the stv locus does not ubiquitously respond to thermal stress but is expressed as three distinct thermal and temporal RNA phenotypes (isoforms). The shorter transcript isoforms are rapidly upregulated under stress in all populations and show similar molecular signatures to heat-shock proteins. Multiple stress exposures seem to generate a reserve of pre-mRNAs, effectively 'priming' the cells for subsequent stress. Remarkably, we demonstrate a bypass in the splicing blockade in these isoforms, suggesting an essential role for these transcripts under heat stress. Temporal profiles for the weakly heat responsive stv isoform subset show opposing patterns in the two most divergent populations. Innate and induced transcriptome responses to hyperthermia are complex, and warrant moving beyond gene-level analyses.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Telonis-Scott M,Clemson AS,Johnson TK,Sgrò CMdoi
10.1111/mec.13000subject
Has Abstractpub_date
2014-12-01 00:00:00pages
6135-51issue
24eissn
0962-1083issn
1365-294Xjournal_volume
23pub_type
杂志文章abstract：:Breeding indigenous African taurine cattle tolerant to trypanosomosis is a straightforward approach to control costs generated by this disease. A recent study identified quantitative trait loci (QTL) underlying trypanotolerance traits in experimental crosses between tolerant N'Dama and susceptible Boran zebu cattle. A...
journal_title：Molecular ecology
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abstract：:While microsatellites have been used to examine genetic structure in local populations of Neotropical trees, genetic studies based on such high-resolution markers have not been carried out for Mesoamerica as a whole. Here we assess the genetic structure of the Mesoamerican mahogany Swietenia macrophylla King (big-leaf...
journal_title：Molecular ecology
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更新日期：2003-11-01 00:00:00
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journal_title：Molecular ecology
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更新日期：2004-10-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
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更新日期：2009-09-01 00:00:00
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更新日期：2003-12-01 00:00:00
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更新日期：2000-10-01 00:00:00
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journal_title：Molecular ecology
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更新日期：2015-12-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/mec.14931
更新日期：2019-02-01 00:00:00
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更新日期：2017-02-01 00:00:00
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更新日期：1995-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/mec.12265
更新日期：2013-05-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/mec.12713
更新日期：2014-04-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/mec.15539
更新日期：2020-08-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/j.1365-294X.2011.05435.x
更新日期：2012-03-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-294X.2012.05538.x
更新日期：2012-04-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1046/j.1365-294x.2002.01483.x
更新日期：2002-04-01 00:00:00
abstract：:Transposable elements are mobile sequences found in nuclear genomes and can potentially serve as molecular markers in various phylogenetic and population genetic investigations. A PCR-based method that utilizes restriction site variation of element copies within a genome is developed. These patterns of site variation,...
journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/j.1365-294x.1995.tb00218.x
更新日期：1995-04-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/j.1365-294x.1996.tb00343.x
更新日期：1996-08-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1046/j.1365-294x.1999.00721.x
更新日期：1999-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/mec.13285
更新日期：2015-08-01 00:00:00
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journal_title：Molecular ecology
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更新日期：2013-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1365-294X.2006.03093.x
更新日期：2006-11-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
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更新日期：2007-02-01 00:00:00
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journal_title：Molecular ecology
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journal_title：Molecular ecology
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doi：10.1111/mec.12200
更新日期：2013-03-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/mec.13811
更新日期：2016-10-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1111/mec.15674
更新日期：2020-10-08 00:00:00
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journal_title：Molecular ecology
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journal_title：Molecular ecology
pub_type： 杂志文章,评审
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更新日期：2008-06-01 00:00:00
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journal_title：Molecular ecology
pub_type： 杂志文章
doi：10.1046/j.1365-294x.2003.01800.x
更新日期：2003-04-01 00:00:00
