Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis.

Abstract:

:The random amplified polymorphic DNA (RAPD) technique was employed in the haplo-diploid dioecious species Gracilaria gracilis to identify sex-linked PCR markers. Sixty-nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid females. One of these primers (OPD13) generated a 430-bp fragment specific to males and a 620-bp fragment specific to females. The diploid individuals (tetrasporophytes) showed the co-occurrence of these two fragments. In order to verify the linkage between the sexual phenotypes and these markers, a progeny array of 59 haploid individuals (male and female) born on a diploid individual was analysed, in all of which the two markers produced by the OPD13 primer segregated perfectly with sex.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Martinez EA,Destombe C,Quillet MC,Valero M

doi

10.1046/j.1365-294x.1999.00721.x

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

1533-8

issue

9

eissn

0962-1083

issn

1365-294X

pii

mec721

journal_volume

8

pub_type

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