Abstract:
:The random amplified polymorphic DNA (RAPD) technique was employed in the haplo-diploid dioecious species Gracilaria gracilis to identify sex-linked PCR markers. Sixty-nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid females. One of these primers (OPD13) generated a 430-bp fragment specific to males and a 620-bp fragment specific to females. The diploid individuals (tetrasporophytes) showed the co-occurrence of these two fragments. In order to verify the linkage between the sexual phenotypes and these markers, a progeny array of 59 haploid individuals (male and female) born on a diploid individual was analysed, in all of which the two markers produced by the OPD13 primer segregated perfectly with sex.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Martinez EA,Destombe C,Quillet MC,Valero Mdoi
10.1046/j.1365-294x.1999.00721.xsubject
Has Abstractpub_date
1999-09-01 00:00:00pages
1533-8issue
9eissn
0962-1083issn
1365-294Xpii
mec721journal_volume
8pub_type
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