Abstract:
INTRODUCTION:In vivo, the airway epithelium stretches and relaxes with each respiratory cycle, but little is known about the effect this pattern of elongation and relaxation has on bronchial epithelial cells. We have used a model of cell deformation to measure the effect of stretch on inflammatory cytokine release by the BEAS 2B cell line, and to examine the method of mechanotransduction in these cells. METHODS:BEAS 2B cells were cyclically stretched using the Flexercell system. IL-8 and RANTES protein and RNA levels were measured after different elongations, rates and duration of stretch. An inhibitor of Rho (Ras Homologous)-associated kinases was used, to assess the effect of blocking downstream of integrin signalling. Immunofluorescent staining of paxillin was used to study the effect of stretch on the distribution of focal contacts and the organisation of the actin cytoskeleton. RESULTS:IL-8 release by BEAS 2B cells was increased by cytokine stimulation and stretch, whereas RANTES levels in the cell supernatant decreased after stretch in a dose-, time- and rate-dependent manner. Thirty percent elongation at 20 cycles/min for 24h increased IL-8 levels by over 100% (P < 0.01). Blocking rho kinase using Y-27632 inhibited the effect of stretch on IL-8 release by the BEAS 2B cells. Immunofluorescent staining demonstrated that stretch caused dramatic disassembly of focal adhesions and resulted in the redistribution of paxillin to the peri-nuclear region. CONCLUSION:This study demonstrates a marked effect of stretch on bronchial epithelial cell function. We propose that stretch modulates epithelial cell function via the activation of rho kinases. The observation that stretch promotes focal adhesion disassembly suggests a mechanism whereby focal adhesion turnover (coordination of assembly and disassembly) is essential for mechanotransduction in bronchial epithelial cells.
journal_name
Respir Medjournal_title
Respiratory medicineauthors
Thomas RA,Norman JC,Huynh TT,Williams B,Bolton SJ,Wardlaw AJdoi
10.1016/j.rmed.2005.12.008subject
Has Abstractpub_date
2006-09-01 00:00:00pages
1588-97issue
9eissn
0954-6111issn
1532-3064pii
S0954-6111(05)00553-6journal_volume
100pub_type
杂志文章abstract:OBJECTIVE:To assess whether tumor necrosis factor (TNF) antagonism can attenuate eosinophilic airway inflammation in patients with mild-to-moderate allergic asthma. DESIGN:Randomized, double-blind, placebo-controlled trial. SETTING:National Institutes of Health (NIH) Clinical Center. PATIENTS:Twenty-six patients wit...
journal_title:Respiratory medicine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.rmed.2005.02.031
更新日期:2005-09-01 00:00:00
abstract::The aim of this study was to examine an acoustic reflection method using a flexible tube for identifying the obstructive site of the upper airway in snorers and patients with obstructive sleep apnoea (OSA). As a preliminary study it was performed n models and subjects in the awake state. Flextube narrowing was produce...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1053/rmed.2001.1116
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journal_title:Respiratory medicine
pub_type: 杂志文章,meta分析,评审
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更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:The 6-min walk test (6MWT) is a submaximal exercise test used to assess functional status in patients with cardiopulmonary diseases. Regression equations have previously been published as a normal reference for 6-min walk distance (6MWD) in healthy Caucasian adults. However, a recent study showed that the no...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2009.01.012
更新日期:2009-07-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2020.105955
更新日期:2020-05-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/s0954-6111(08)80087-x
更新日期:1990-01-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2013.12.012
更新日期:2014-03-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2020.106062
更新日期:2020-01-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/s0954-6111(97)90083-4
更新日期:1997-10-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1053/rmed.2001.1187
更新日期:2001-12-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2009.04.029
更新日期:2009-11-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/s0954-6111(98)90002-6
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:Changes in management of COPD in primary care were studied following a training exercise among Spanish general practitioners (GPs). The exercise involved dissemination of the Spanish Society for Pulmonology and Thoracic Surgery (SEPAR) guidelines. The use of a portable device to perform spirometry tests was ...
journal_title:Respiratory medicine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.rmed.2009.08.003
更新日期:2010-01-01 00:00:00
abstract::Intrapulmonary percussive ventilation (IPV) is a ventilatory technique that delivers bursts of high-flow respiratory gas into the lung at high rates, intended for treating acute respiratory failure and for mobilization of secretions. We performed a study, aimed at assessing the physiological response to IPV, on patien...
journal_title:Respiratory medicine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.rmed.2006.01.010
更新日期:2006-09-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.rmed.2010.08.005
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:The diagnosis of pulmonary aspergillosis is difficult because the sensitivity of the conventional methods for the detection of Aspergillus such as culture and cytology, is poor. To improve the sensitivity for Aspergillus detection, the detection of galactomannan antigen has been investigated. The serum galac...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2013.04.007
更新日期:2013-07-01 00:00:00
abstract::We have analysed the clinical manifestations of nine patients with brief upper airway dysfunction (BUAD) who attended the thoracic department of a major teaching hospital between 1987 and 1991. Episodes of BUAD developed within 1-4 months of presentation in three patients but were undiagnosed for 2.5-12.5 years in six...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/0954-6111(94)90024-8
更新日期:1994-02-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1053/rmed.2001.1053
更新日期:2001-07-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2017.11.004
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible interstitial lung disease with poor prognosis despite the recent availability of two antifibrotic drugs. Patients are more susceptible to cardiovascular comorbidities. In this study, we aimed to determine the impact of concom...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2020.106259
更新日期:2020-11-26 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.rmed.2017.03.017
更新日期:2017-05-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2007.09.004
更新日期:2008-02-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1053/rmed.2000.0959
更新日期:2000-12-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2009.11.014
更新日期:2010-05-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2012.03.011
更新日期:2012-07-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2004.02.022
更新日期:2004-09-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.rmed.2019.08.001
更新日期:2019-09-01 00:00:00
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journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/j.rmed.2017.06.004
更新日期:2017-11-01 00:00:00
abstract::A survey was undertaken to determine the current practice in the Yorkshire Health Region for the assessment and provision of home nebulizers for adults with chronic obstructive pulmonary disease (COPD). A detailed postal questionnaire on assessment methods was sent to each of the 22 consultant physicians in respirator...
journal_title:Respiratory medicine
pub_type: 杂志文章
doi:10.1016/0954-6111(95)90070-5
更新日期:1995-01-01 00:00:00