Three mechanisms in the pathogenesis of pre-eclampsia suggested by over-represented transcription factor-binding sites detected with comparative promoter analysis.

abstract：:Preeclampsia (PE) is a severe pregnancy-induced disorder characterized by hypertension and proteinuria and a leading cause of perinatal maternal-fetal mortality and morbidity in developing countries. Dysregulated human leukocyte antigen (HLA)-G was found in placentas as well as in maternal sera from PE patients; howev...

journal_title：Molecular human reproduction

authors： Tang Y,Liu H,Li H,Peng T,Gu W,Li X

更新日期：2015-09-01 00:00:00

abstract：:We describe a rapid and specific differential amplification system which can detect five of the most common cystic fibrosis mutations from a single cell. In the first round of the polymerase chain reaction (PCR), regions of exons 4, 10 and 11 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene conta...

journal_title：Molecular human reproduction

authors： Scobie G,Woodroffe B,Fishel S,Kalsheker N

更新日期：1996-03-01 00:00:00

abstract：:Success rate in human pregnancies is believed to be very low and sex-specific mechanisms may operate in prenatal loss. Assuming a sex-differential in prenatal loss exists, we examined genetic markers in biologically plausible targets in the HLA complex, other immune system-related and iron-regulatory genes in 388 heal...

journal_title：Molecular human reproduction

authors： Ucisik-Akkaya E,Davis CF,Do TN,Morrison BA,Stemmer SM,Amadio WJ,Dorak MT

更新日期：2010-10-01 00:00:00

abstract：:Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic gen...

journal_title：Molecular human reproduction

authors： Bell CE,Calder MD,Watson AJ

更新日期：2008-12-01 00:00:00

abstract：:Development of uterine glands (adenogenesis) in mammals typically begins during the early post-natal period and involves budding of nascent glands from the luminal epithelium and extensive cell proliferation in these structures as they grow into the surrounding stroma, elongate and mature. Uterine glands are essential...

journal_title：Molecular human reproduction

authors： Cooke PS,Spencer TE,Bartol FF,Hayashi K

更新日期：2013-09-01 00:00:00

abstract：:Human mitochondrial DNA (mtDNA) rearrangements, including more than 150 deletions and insertions, accumulate with age and are responsible for certain neuromuscular diseases. Human oocytes, arrested for up to 50 years, may express certain mtDNA rearrangements possibly affecting function. Investigations have previously ...

journal_title：Molecular human reproduction

authors： Barritt JA,Brenner CA,Cohen J,Matt DW

更新日期：1999-10-01 00:00:00

abstract：:Previous studies have implicated receptor tyrosine kinases in progesterone-induced [Ca2+]i signalling, and consequent induction of the acrosome reaction, in human spermatozoa. We have investigated the effects of tyrosine kinase inhibition on [Ca2+]i responses in large numbers of individual human spermatozoa. Genistein...

journal_title：Molecular human reproduction

authors： Kirkman-Brown JC,Lefièvre L,Bray C,Stewart PM,Barratt CL,Publicover SJ

更新日期：2002-04-01 00:00:00

abstract：:The process of sperm chromatin decondensation occurs when a spermatozoon enters an ovum. Protamine disulphide bonds are reduced to SH and the polycationic protamines combine with the polyanionic egg protein, nucleoplasmin, thus being stripped from DNA which then combines with histones. Defective chromatin decondensati...

journal_title：Molecular human reproduction

authors： Samocha-Bone D,Lewin LM,Weissenberg R,Madgar Y,Soffer Y,Shochat L,Golan R

更新日期：1998-02-01 00:00:00

abstract：:Cytogenetically, uterine leiomyomata are the best investigated human tumours. The most frequent clonal abnormalities are structural rearrangements involving 12q14-15 and deletions of part of the long arm of chromosome 7. The present study investigated a possible growth advantage conferred by these abnormalities, when ...

journal_title：Molecular human reproduction

authors： Hennig Y,Deichert U,Bonk U,Thode B,Bartnitzke S,Bullerdiek J

更新日期：1999-12-01 00:00:00

abstract：:SUN domain proteins are integral proteins of the inner nuclear membrane and functions in linkage of the nuclear lamina to the cytoskeleton. Moreover, SUN domain proteins seem to mediate the tethering of the centrosome to the nuclear membrane, and they are involved in telomere attachment to the nuclear envelope in meio...

journal_title：Molecular human reproduction

authors： Frohnert C,Schweizer S,Hoyer-Fender S

更新日期：2011-04-01 00:00:00

abstract：:The present study was undertaken to investigate the effect of decidualization on superoxide dismutase (SOD) expression in human endometrial stromal cells (ESC). To induce decidualization, isolated ESC were incubated with medroxyprogesterone acetate (MPA, 10(-6) mol/l) and oestradiol (10(-8) mol/l) for 23 days. Insulin...

journal_title：Molecular human reproduction

authors： Sugino N,Kashida S,Takiguchi S,Nakamura Y,Kato H

更新日期：2000-02-01 00:00:00

abstract：:Sperm are motile cells. Thus, a significant component of the spermatogenic cycle is devoted to the formation of flagellum, a process that must be coordinated to insure proper construction. To document the temporal pattern of flagellar gene expression, we employed real-time PCR to assess changes in accumulation of a co...

journal_title：Molecular human reproduction

authors： Horowitz E,Zhang Z,Jones BH,Moss SB,Ho C,Wood JR,Wang X,Sammel MD,Strauss JF 3rd

更新日期：2005-04-01 00:00:00

abstract：:A major challenge in the comprehension of the endometrial transformations leading to the completion of each menstrual cycle in humans is in the identification of specific molecular pathways underlying these monthly turnovers. Towards this goal we compared, by the differential display technique, the relative expression...

journal_title：Molecular human reproduction

authors： Malette B,Cherry E,Lagacé M,Bernard M,Gosselin D,Hugo P,Shazand K

更新日期：2003-11-01 00:00:00

abstract：:Human pregnancy represents a situation of semiallograft to maternal host. Therefore, it has been reported that tolerance to the fetal allograft represents a mechanism for maintaining a pregnancy. CD4(+)CD25(bright) regulatory T cells are known to play an important role in the development and maintenance of tolerance i...

journal_title：Molecular human reproduction

authors： Sasaki Y,Sakai M,Miyazaki S,Higuma S,Shiozaki A,Saito S

更新日期：2004-05-01 00:00:00

abstract：:The influence of seminal plasma on the mRNA expression of cytokines in human endometrial epithelial and stromal cells and the cytokine production of spermatozoa were investigated in vitro. Seminal plasma and spermatozoa were collected from healthy volunteers and were screened by enzyme-linked immunosorbent assay for c...

journal_title：Molecular human reproduction

authors： Gutsche S,von Wolff M,Strowitzki T,Thaler CJ

更新日期：2003-12-01 00:00:00

abstract：:The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied ...

journal_title：Molecular human reproduction

authors： Pilka R,Whatling C,Domanski H,Hansson S,Eriksson P,Casslén B

更新日期：2003-05-01 00:00:00

abstract：:The aim of this study was to determine the effects of tumour necrosis factor alpha (TNF), interleukin-1 alpha (IL-1alpha), macrophage colony-stimulating factor (MCSF) and transforming growth factor beta (TGFbeta) on the secretion of matrix metalloproteinases (MMP), human chorionic gonadotrophin (HCG) and fetal fibrone...

journal_title：Molecular human reproduction

authors： Meisser A,Chardonnens D,Campana A,Bischof P

更新日期：1999-03-01 00:00:00

abstract：:Within the context of an oocyte in vitro maturation (IVM) program for reproductive treatment, oocyte cumulus complexes (COCs) derived from follicles <6 mm in patients with PCOS were matured in vitro. Key transcripts related to meiotic maturation (FSHR, LHCGR, EGFR, PGR) and oocyte competence (AREG, ADAMTS, HAS2, PTGS2...

journal_title：Molecular human reproduction

authors： Guzman L,Adriaenssens T,Ortega-Hrepich C,Albuz FK,Mateizel I,Devroey P,De Vos M,Smitz J

更新日期：2013-01-01 00:00:00

abstract：STUDY QUESTION:What is the acceptability of using stem cell-based fertility treatments (SCFT) for different indications according to gynaecologists and the general public? SUMMARY ANSWER:The majority of gynaecologists and the general public accept SCFT for the indications female or male infertility in young heterosexu...

journal_title：Molecular human reproduction

authors： Hendriks S,Dancet EAF,Vliegenthart R,Repping S

更新日期：2017-12-01 00:00:00

abstract：:Mammalian sperm chromatin is highly condensed, so isolating DNA from such chromatin can be a formidable task. The procedures that produce high quality DNA from somatic cells fail to yield quality sperm DNA. In this study we have modified the previously used guanidinium method to make it simple and efficient in isolati...

journal_title：Molecular human reproduction

authors： Hossain AM,Rizk B,Behzadian A,Thorneycroft IH

更新日期：1997-11-01 00:00:00

abstract：:The requisite endpoint of mammalian oocyte maturation, whether in vivo or in vitro, is a metaphase II oocyte which is able to be fertilized and which can eventually support normal embryonic development. Oocytes which have been matured in vivo basically fulfill these criteria. On the other hand, a completely different ...

journal_title：Molecular human reproduction

authors： Fulka J Jr,First NL,Moor RM

更新日期：1998-01-01 00:00:00

abstract：:Prostaglandins (PGs) are important factors in the physiology of human parturition and the control of uterine contractility. We have characterized the expression of 15 genes from all stages of the PG pathway in human pregnant and non-pregnant (NP) myometrium and in other uterine tissues at delivery, and the results sho...

journal_title：Molecular human reproduction

authors： Phillips RJ,Al-Zamil H,Hunt LP,Fortier MA,López Bernal A

更新日期：2011-01-01 00:00:00

abstract：:The objectives of this study were to determine if human ejaculated sperm exhibit active caspases and if caspase-dependent apoptosis markers are identifiable. Sperm from fertile donors and infertile patients were examined after gradient separation into leukocyte-free fractions of high and low motility. Sperm were evalu...

journal_title：Molecular human reproduction

authors： Weng SL,Taylor SL,Morshedi M,Schuffner A,Duran EH,Beebe S,Oehninger S

更新日期：2002-11-01 00:00:00

abstract：:We aimed to confirm the ovarian site of gonadotrophin surge-attenuating factor (GnSAF) production and produce granulosa/luteal cell-conditioned medium (G/LCM) containing GnSAF for purification studies. Blue dye affinity chromatography followed by pseudochromatofocusing of G/LCM yielded bioactive fractions at pH 5.74 a...

journal_title：Molecular human reproduction

authors： Fowler PA,Sorsa-Leslie T,Cash P,Dunbar B,Melvin W,Wilson Y,Mason HD,Harris W

更新日期：2002-09-01 00:00:00

abstract：:The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze molecular effects of systemic in vivo blockade of α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance of cAMP/cGMP signaling in testosterone-producing Leyd...

journal_title：Molecular human reproduction

authors： Stojkov NJ,Baburski AZ,Bjelic MM,Sokanovic SJ,Mihajlovic AI,Drljaca DM,Janjic MM,Kostic TS,Andric SA

更新日期：2014-01-01 00:00:00

abstract：:In mammals, egg activation is initiated by multiple cytosolic Ca(2+) transients (Ca(2+) oscillations) that are triggered following delivery of a putative sperm factor from the fertilizing sperm. The identity of this 'sperm factor' thus holds much significance, not only as a vital component in creating a new life, but ...

journal_title：Molecular human reproduction

authors： Kashir J,Nomikos M,Swann K,Lai FA

更新日期：2015-05-01 00:00:00

abstract：:Recent data demonstrated that CD4+CD25+ regulatory T (Treg) cells and an enzyme called indoleamine 2,3-dioxygenase (IDO) mediate maternal tolerance to the fetus. Interestingly, Treg cells express the CTLA-4 molecule on their surface, and B7 (CD80/86) ligation by CTLA-4 enhanced IDO activity of dendritic cells (DCs) an...

journal_title：Molecular human reproduction

authors： Miwa N,Hayakawa S,Miyazaki S,Myojo S,Sasaki Y,Sakai M,Takikawa O,Saito S

更新日期：2005-12-01 00:00:00

abstract：:Testis expressed gene 18 (Tex18) is a small gene with one exon of 240 bp, which is specifically expressed in male germ cells. The gene encodes for a protein of 80 amino acids with unknown domain. To investigate the function of (Tex18) gene, we generated mice with targeted disruption of the (Tex18) gene by homologous r...

journal_title：Molecular human reproduction

authors： Jaroszynski L,Dev A,Li M,Meinhardt A,de Rooij DG,Mueller C,Böhm D,Wolf S,Adham IM,Wulf G,Engel W,Nayernia K

更新日期：2007-03-01 00:00:00

abstract：:The pool of primordial follicles in humans is laid down during embryonic development and follicles can remain dormant for prolonged intervals, often decades, until individual follicles resume growth. The mechanisms that induce growth and maturation of primordial follicles are poorly understood but follicles once activ...

journal_title：Molecular human reproduction

authors： Markholt S,Grøndahl ML,Ernst EH,Andersen CY,Ernst E,Lykke-Hartmann K

更新日期：2012-02-01 00:00:00

abstract：:During pregnancy, a cervical connective tissue remodelling takes place, clinically recognized as softening, effacement and dilatation. The role of oestrogens and their receptors (ER) in this process is not clear. ERalpha is a ligand-activated transcription factor involved in many physiological processes. The identific...

journal_title：Molecular human reproduction

authors： Wang H,Stjernholm Y,Ekman G,Eriksson H,Sahlin L

更新日期：2001-03-01 00:00:00