Abstract:
:A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 microg ml(-1)). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM1443 (pJP4::gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4::gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacI(q) repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 microm in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Aspray TJ,Hansen SK,Burns RGdoi
10.1016/j.femsec.2005.04.007subject
Has Abstractpub_date
2005-10-01 00:00:00pages
317-27issue
2eissn
0168-6496issn
1574-6941pii
S0168-6496(05)00118-2journal_volume
54pub_type
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