A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4.

Abstract:

:A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 microg ml(-1)). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM1443 (pJP4::gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4::gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacI(q) repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 microm in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia.

journal_name

FEMS Microbiol Ecol

authors

Aspray TJ,Hansen SK,Burns RG

doi

10.1016/j.femsec.2005.04.007

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

317-27

issue

2

eissn

0168-6496

issn

1574-6941

pii

S0168-6496(05)00118-2

journal_volume

54

pub_type

杂志文章
  • Impact of streambed morphology on the abundance and activity of ammonia-oxidizing bacteria.

    abstract::Ammonia oxidizers catalyze the first step of nitrification. Combined microbial nitrification-denitrification activities are essential for the removal of excess nitrogen from water bodies. In sandy streambeds, bed form structures are created by water flow and lead to the creation of heterogeneous microenvironments. The...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12385

    authors: Yanuka-Golub K,Arnon S,Nejidat A

    更新日期:2014-10-01 00:00:00

  • Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods.

    abstract::Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surfa...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.07.010

    authors: Süss J,Engelen B,Cypionka H,Sass H

    更新日期:2004-12-27 00:00:00

  • Bacterial community profile after the lethal infection of Steinernema-Xenorhabdus pairs into soil-reared Tenebrio molitor larvae.

    abstract::The host microbiota may have an impact on pathogens. This is often studied in laboratory-reared hosts but rarely in individuals whose microbiota looks like that of wild animals. In this study, we modified the gut microbiota of the insect Tenebrio molitor by rearing larvae in soil sampled from the field. We showed by h...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa009

    authors: Cambon MC,Lafont P,Frayssinet M,Lanois A,Ogier JC,Pagès S,Parthuisot N,Ferdy JB,Gaudriault S

    更新日期:2020-02-01 00:00:00

  • A dietary polyunsaturated fatty acid improves consumer performance during challenge with an opportunistic bacterial pathogen.

    abstract::A dietary deficiency in polyunsaturated fatty acids (PUFAs) and/or sterols can severely constrain growth and reproduction of invertebrate consumers. Single nutrients are potentially assigned to different physiological processes, for example to support defence mechanisms; therefore, lipid requirements of healthy and pa...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12407

    authors: Schlotz N,Pester M,Freese HM,Martin-Creuzburg D

    更新日期:2014-11-01 00:00:00

  • Diversity and function of soil microbes on montane gradients: the state of knowledge in a changing world.

    abstract::Mountains have a long history in the study of diversity. Like macroscopic taxa, soil microbes are hypothesized to be strongly structured by montane gradients, and recently there has been important progress in understanding how microbes are shaped by these conditions. Here, we summarize this literature and synthesize p...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa122

    authors: Looby CI,Martin PH

    更新日期:2020-09-01 00:00:00

  • Thermophilic methanotrophs: in hot pursuit.

    abstract::Methane is a potent greenhouse gas responsible for 20-30% of global climate change effects. The global methane budget is ∼500-600 Tg y-1, with the majority of methane produced via microbial processes, including anthropogenic-mediated sources such as ruminant animals, rice fields, sewage treatment facilities and landfi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审

    doi:10.1093/femsec/fiz125

    authors: Houghton KM,Carere CR,Stott MB,McDonald IR

    更新日期:2019-09-01 00:00:00

  • The enrichment of an alkaliphilic biofilm consortia capable of the anaerobic degradation of isosaccharinic acid from cellulosic materials incubated within an anthropogenic, hyperalkaline environment.

    abstract::Anthropogenic hyperalkaline sites provide an environment that is analogous to proposed cementitious geological disposal facilities (GDF) for radioactive waste. Under anoxic, alkaline conditions cellulosic wastes will hydrolyze to a range of cellulose degradation products (CDP) dominated by isosaccharinic acids (ISA). ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv085

    authors: Charles CJ,Rout SP,Garratt EJ,Patel K,Laws AP,Humphreys PN

    更新日期:2015-08-01 00:00:00

  • Reduction in denitrification activity in field soils exposed to long term contamination by 2,4,6-trinitrotoluene (TNT).

    abstract::Terrestrial sites contaminated with 2,4,6-trinitrotoluene (TNT) are a widespread and persistent problem and often contain non-vegetated areas with TNT concentrations well in excess of 1000 mg kg(-1). In this study, we examined the effect of TNT on denitrification activity in field soils, and compared the sensitivity o...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2000.tb00699.x

    authors: Siciliano SD,Roy R,Greer CW

    更新日期:2000-04-01 00:00:00

  • Mimosa caesalpiniifolia Benth. adapts to rhizobia populations with differential taxonomy and symbiotic effectiveness outside of its location of origin.

    abstract::Mimosa caesalpiniifolia Benth. is a legume native to the semi-arid region of Brazil, in the Northeast. Its successful adaptation to other locations, such as the Atlantic Forest in the Southeast region, may be related to its ability to establish symbiosis with nitrogen-fixing bacteria, especially β-rhizobia of the genu...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz109

    authors: de Oliveira ISR,Jesus EDC,Ribeiro TG,Santos Reis de Andrade da Silva M,Tenorio JO,Martins LMV,de Faria SM

    更新日期:2019-08-01 00:00:00

  • Impacts of heavy metal contamination and phytoremediation on a microbial community during a twelve-month microcosm experiment.

    abstract::The effects of heavy metals and phytoextraction practices on a soil microbial community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulescens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bacteria and the beta-Proteobacteria and the amoA gene (encoding th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.02.004

    authors: Gremion F,Chatzinotas A,Kaufmann K,Von Sigler W,Harms H

    更新日期:2004-05-01 00:00:00

  • An individual-based approach to explain plasmid invasion in bacterial populations.

    abstract::We present an individual-based experimental framework to identify and estimate the main parameters governing bacterial conjugation at the individual cell scale. From this analysis, we have established that transient periods of unregulated plasmid transfer within newly formed transconjugant cells, together with contact...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00994.x

    authors: Seoane J,Yankelevich T,Dechesne A,Merkey B,Sternberg C,Smets BF

    更新日期:2011-01-01 00:00:00

  • Geographical patterns of root nodule bacterial diversity in cultivated and wild populations of a woody legume crop.

    abstract::There is interest in understanding how cultivation, plant genotype, climate and soil conditions influence the biogeography of root nodule bacterial communities of legumes. For crops from regions with relict wild populations, this is of even greater interest because the effects of cultivation on symbiont communities ca...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa145

    authors: Ramoneda J,Roux JJL,Frossard E,Frey B,Gamper HA

    更新日期:2020-10-01 00:00:00

  • Spatial and temporal changes of cyanophage communities in paddy field soils as revealed by the capsid assembly protein gene g20.

    abstract::Bacteriophages are ubiquitous in various environments. Our previous study revealed the diversity of the cyanophage community in paddy floodwater. In this study, the phylogeny and genetic diversity of cyanophage communities in paddy field soils were reported. The viral capsid assembly protein gene (g20) of cyanophage w...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01052.x

    authors: Wang G,Asakawa S,Kimura M

    更新日期:2011-05-01 00:00:00

  • Mitochondrial sequences of Rhipicephalus and Coxiella endosymbiont reveal evidence of lineages co-cladogenesis.

    abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa072

    authors: Coimbra-Dores MJ,Jaarsma RI,Carmo AO,Maia-Silva M,Fonville M,da Costa DFF,Brandão RML,Azevedo F,Casero M,Oliveira AC,Afonso SMS,Sprong H,Rosa F,Dias D

    更新日期:2020-06-01 00:00:00

  • Carbohydrate active enzymes are affected by diet transition from milk to solid food in infant gut microbiota.

    abstract::Infants experience a dramatic change in their food in the first year after birth when they shift from breast milk to solid food. This results in a large change in presence of indigestible polysaccharides, a primary energy resource of gut microbes. How the gut microbiota adapts to this dietary shift has not been well e...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz159

    authors: Ye L,Das P,Li P,Ji B,Nielsen J

    更新日期:2019-11-01 00:00:00

  • Two-years of investigation revealed the inconsistency of seasonal dynamics of an ectomycorrhizal fungal community in Japanese cool-temperate forest across years.

    abstract::Ectomycorrhizal (ECM) fungal communities show temporal dynamics. Such dynamics have been mainly assessed with 1 year of investigations and have been related to the seasonal changes in environment. Recent study in sub-tropical region has revealed that stochastic temporal-based process can affect ECM fungal community, m...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa118

    authors: Sugiyama Y,Matsuoka S,Osono T

    更新日期:2020-07-01 00:00:00

  • Fecal bacterial communities of wild-captured and stranded green turtles (Chelonia mydas) on the Great Barrier Reef.

    abstract::Green turtles (Chelonia mydas) are endangered marine herbivores that break down food particles, primarily sea grasses, through microbial fermentation. However, the microbial community and its role in health and disease is still largely unexplored. In this study, we investigated and compared the fecal bacterial communi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix139

    authors: Ahasan MS,Waltzek TB,Huerlimann R,Ariel E

    更新日期:2017-12-01 00:00:00

  • Ectomycorrhizal hyphae structure components of the soil bacterial community for decreased phosphatase production.

    abstract::Ectomycorrhizal fungi (EMF) provide nutrients to their hosts by means of hyphae that extend beyond nutrient-depleted rhizosphere soil. Soil bacteria may compete with EMF for nutrients or may act synergistically to enhance nutrient supply to hosts. To assess the interactions between hyphae and bacteria, two types of sm...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01060.x

    authors: Brooks DD,Chan R,Starks ER,Grayston SJ,Jones MD

    更新日期:2011-05-01 00:00:00

  • Endolithic photosynthetic communities within ancient and recent travertine deposits in Yellowstone National Park.

    abstract::Molecular and culture based methods were used to survey endolithic, photosynthetic communities from hot spring-formed travertine rocks of various ages, ranging from<10 to greater than 300,000 years. Much of this travertine contained a 1-3-mm-thick greenish band composed mainly of cyanobacteria 1-5 mm below the rock su...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00134.x

    authors: Norris TB,Castenholz RW

    更新日期:2006-09-01 00:00:00

  • Methane release from sediment seeps to the atmosphere is counteracted by highly active Methylococcaceae in the water column of deep oligotrophic Lake Constance.

    abstract::Methane emissions from freshwater environments contribute substantially to global warming but are under strong control of aerobic methane-oxidizing bacteria. Recently discovered methane seeps (pockmarks) in freshwater lake sediments have the potential to bypass this control by their strong outgassing activity. Whether...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw123

    authors: Bornemann M,Bussmann I,Tichy L,Deutzmann J,Schink B,Pester M

    更新日期:2016-08-01 00:00:00

  • Whole genome sequencing and antibiotic diffusion assays, provide new insight on drug resistance in the genus Pedobacter.

    abstract::A total of four strains of the 'environmental superbug' Pedobacter isolated from sludge produced at Norwegian drinking water treatment plants, were characterized by whole genome sequencing and antibiotic susceptibility assays. As with previous studies on members of this genus, we found that the isolates were multi-dru...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa088

    authors: Ullmann IF,Nygaard AB,Tunsjø HS,Charnock C

    更新日期:2020-06-01 00:00:00

  • Effects of endophytic fungi on the ash dieback pathogen.

    abstract::While Hymenoscyphus fraxineus causes dieback of the European ash (Fraxinus excelsior), flowering ash (F. ornus) appears resistant to the pathogen. To date, contributions of endophytic fungi to host resistance are unknown. The following hypotheses were tested: (i) endophytic fungi enhance the resistance of F. excelsior...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw142

    authors: Schlegel M,Dubach V,von Buol L,Sieber TN

    更新日期:2016-09-01 00:00:00

  • Characterization of the bacterial community composition in a hypoxic zone induced by Microcystis blooms in Lake Taihu, China.

    abstract::Cyanobacterial blooms have become more frequent as a result of eutrophication in lakes. The accumulation and breakdown of huge cyanobacterial biomasses often cause hypoxia in lakes. However, little is known about microbial diversity in these areas. In this study, we characterized the bacterial community composition of...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01262.x

    authors: Li H,Xing P,Wu QL

    更新日期:2012-03-01 00:00:00

  • Thermus diversity and strain loss during enrichment.

    abstract::The diversity of Thermus strains isolated from each of two New Zealand hot pools was examined by isolating partial SSU (16S) rRNA genes and comparing their sequences. Although all of the sequences were similar, several variants were found in each pool. Standard methods for the enrichment of Thermus were then carried o...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.1999.tb00644.x

    authors: Saul DJ,Reeves RA,Morgan HW,Bergquist PL

    更新日期:1999-10-01 00:00:00

  • Soil fungal community composition and functional similarity shift across distinct climatic conditions.

    abstract::A large part of ecosystem function in woodland systems depends on soil fungal communities. However, global climate change has the potential to fundamentally alter these communities as fungal species are filtered with changing environmental conditions. In this study, we examined the potential effects of climate on host...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa193

    authors: Bui A,Orr D,Lepori-Bui M,Konicek K,Young HS,Moeller HV

    更新日期:2020-12-02 00:00:00

  • Occurrence and expression of bacterial human virulence gene homologues in natural soil bacteria.

    abstract::The presence and in vitro expression of homologues to 22 bacterial human virulence determinants amongst culturable soil bacteria were investigated. About 25% of the bacterial isolates contained virulence gene homologues representing toxin (hblA, cytK2), adhesin (fimH), regulator (phoQ) and resistance (yfbI) determinan...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12413

    authors: Søborg DA,Hendriksen NB,Kroer N

    更新日期:2014-11-01 00:00:00

  • Microbial community analysis of deeply buried marine sediments of the New Jersey shallow shelf (IODP Expedition 313).

    abstract::The investigated deeply buried marine sediments of the shallow shelf off New Jersey, USA, are characterized by low organic carbon content and total cell counts of < 10(7) cells per mL sediment. The qPCR data for Bacteria and Archaea were in the same orders of magnitude as the total cell counts. Archaea and Bacteria oc...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12146

    authors: Breuker A,Stadler S,Schippers A

    更新日期:2013-09-01 00:00:00

  • Subterranean microbial populations metabolize hydrogen and acetate under in situ conditions in granitic groundwater at 450 m depth in the Äspö Hard Rock Laboratory, Sweden.

    abstract::Pressure-resistant circulating systems were constructed to enable the investigation of attached and unattached microbial populations under in situ pressure (2.5 MPa), diversity, dissolved gas and chemistry conditions. Three parallel flow cell cabinets were configured to allow observation of the effect on microbial met...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2012.01370.x

    authors: Pedersen K

    更新日期:2012-07-01 00:00:00

  • Bacterial tolerances to metals and antibiotics in metal-contaminated and reference streams.

    abstract::Anthropogenic-derived sources of selection are typically implicated as mechanisms for maintaining antibiotic resistance in the environment. Here we report an additional mechanism for maintaining antibiotic resistance in the environment through bacterial exposure to metals. Using a culture-independent approach, bacteri...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00154.x

    authors: Wright MS,Loeffler Peltier G,Stepanauskas R,McArthur JV

    更新日期:2006-11-01 00:00:00

  • Examination of Gould's modified S1 (mS1) selective medium and Angle's non-selective medium for describing the diversity of Pseudomonas spp. in soil and root environments.

    abstract::Abstract Studies on the diversity of environmental culturable Pseudomonas populations are dependent on the isolation procedure. This procedure includes the use of selective media which may influence the recovery of strains and thus the diversity described. In this study, we assessed the use of two agar isolation media...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00130-2

    authors: Tarnawski S,Hamelin J,Locatelli L,Aragno M,Fromin N

    更新日期:2003-07-01 00:00:00