Canonical Notch signaling is dispensable for early cell fate specifications in mammals.

abstract：:The M double-stranded RNA component of type 1 killer strains of the yeast Saccharomyces cerevisiae contains an internal 200-base pair adenine- and uracil-rich region. The plus strands of this viral genomic RNA contain an internal adenine-rich region which allows these strands to bind to polyuridylate-Sepharose as tigh...

journal_title：Molecular and cellular biology

authors： Hannig EM,Thiele DJ,Leibowitz MJ

更新日期：1984-01-01 00:00:00

abstract：:Addition of serum to mitogen-starved cells activates the cellular immediate-early gene (IEG) response. Serum response factor (SRF) contributes to such mitogen-stimulated transcriptional induction of many IEGs during the G0-G1 cell cycle transition. SRF is also believed to be essential for cell cycle progression, as im...

journal_title：Molecular and cellular biology

authors： Schratt G,Weinhold B,Lundberg AS,Schuck S,Berger J,Schwarz H,Weinberg RA,Rüther U,Nordheim A

更新日期：2001-04-01 00:00:00

abstract：:The ESC-E(Z) complex of Drosophila melanogaster Polycomb group (PcG) repressors is a histone H3 methyltransferase (HMTase). This complex silences fly Hox genes, and related HMTases control germ line development in worms, flowering in plants, and X inactivation in mammals. The fly complex contains a catalytic SET domai...

journal_title：Molecular and cellular biology

authors： Ketel CS,Andersen EF,Vargas ML,Suh J,Strome S,Simon JA

更新日期：2005-08-01 00:00:00

abstract：:Mitochondria are indispensable organelles implicated in multiple aspects of cellular processes, including tumorigenesis. Heat shock proteins play a critical regulatory role in accurately delivering the nucleus-encoded proteins through membrane-bound presequence translocase (Tim23 complex) machinery. Although altered e...

journal_title：Molecular and cellular biology

authors： Sinha D,Srivastava S,Krishna L,D'Silva P

更新日期：2014-05-01 00:00:00

abstract：:To modulate transcription, regulatory factors communicate with basal transcription factors and/or RNA polymerases in a variety of ways. Previously, it has been reported that RNA polymerase II subunit 5 (RPB5) is one of the targets of hepatitis B virus X protein (HBx) and that both HBx and RPB5 specifically interact wi...

journal_title：Molecular and cellular biology

authors： Dorjsuren D,Lin Y,Wei W,Yamashita T,Nomura T,Hayashi N,Murakami S

更新日期：1998-12-01 00:00:00

abstract：:DNA in the polyploid macronucleus of the ciliated protozoan Tetrahymena thermophila contains the modified base N6-methyladenine. We identified two GATC sites which are methylated in most or all of the 45 copies of the macronuclear genome. One site is 2 kilobases 5' to the histone H4-I gene, and the other is 5 kilobase...

journal_title：Molecular and cellular biology

authors： Harrison GS,Findly RC,Karrer KM

更新日期：1986-07-01 00:00:00

abstract：:A murine monoclonal antibody was identified by its ability to induce a reversible antiproliferative effect on a human lymphoma cell line. Immunoprecipitation studies revealed that the antibody reacted with a 26-kilodalton cell surface protein (TAPA-1). A diverse group of human cell lines, including hematolymphoid, neu...

journal_title：Molecular and cellular biology

authors： Oren R,Takahashi S,Doss C,Levy R,Levy S

更新日期：1990-08-01 00:00:00

abstract：:Coordination of skeletal muscle growth and metabolism with nutrient availability is critical for metabolic homeostasis. To establish the role of insulin-like signaling in this process, we used muscle creatine kinase (MCK)-Cre to disrupt expression of insulin receptor substrates Irs1 and Irs2 in mouse skeletal/cardiac ...

journal_title：Molecular and cellular biology

authors： Long YC,Cheng Z,Copps KD,White MF

更新日期：2011-02-01 00:00:00

abstract：:The neutral/neutral two-dimensional (2-D) gel replicon mapping technique has been used to great advantage to localize and characterize origins of replication. Interestingly, many yeast origins display a composite pattern consisting of both a bubble arc and a single-fork arc. Moreover, in every instance in which neutra...

journal_title：Molecular and cellular biology

authors： Kalejta RF,Hamlin JL

更新日期：1996-09-01 00:00:00

abstract：:Hydroxyurea (HU) is a DNA replication inhibitor that negatively affects both the elongation and initiation phases of replication and triggers the "intra-S phase checkpoint." Previous work with budding yeast has shown that, during a short exposure to HU, MEC1/RAD53 prevent initiation at some late S phase origins. In th...

journal_title：Molecular and cellular biology

authors： Alvino GM,Collingwood D,Murphy JM,Delrow J,Brewer BJ,Raghuraman MK

更新日期：2007-09-01 00:00:00

abstract：:Oxygen-dependent proteolysis is the primary means of regulating the hypoxia-inducible factor (HIF) family of transcription factors. The alpha-subunit of HIF factor 1 (HIF-1) contains two highly conserved oxygen-dependent degradation domains (402 ODD and 564 ODD), each of which includes a proline that is hydroxylated i...

journal_title：Molecular and cellular biology

authors： Chan DA,Sutphin PD,Yen SE,Giaccia AJ

更新日期：2005-08-01 00:00:00

abstract：:The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., p...

journal_title：Molecular and cellular biology

authors： Garcia R,Paz-Aliaga B,Ernst SG,Crain WR Jr

更新日期：1984-05-01 00:00:00

abstract：:Sequence blocks within the core region were swapped among RNA polymerase II promoters to explore effects on transcription in vitro. The pair of blocks flanking TATA strongly influenced general transcription, with an additional effect on promoter activation. These flanking elements induced a change in the ratio of acti...

journal_title：Molecular and cellular biology

authors： Wolner BS,Gralla JD

更新日期：2000-05-01 00:00:00

abstract：:Repeat-induced point mutation (RIP) is a process that efficiently detects DNA duplications prior to meiosis in Neurospora crassa and peppers them with G:C to A:T mutations. Cytosine methylation is typically associated with sequences affected by RIP, and methylated cytosines are not limited to CpG dinucleotides. We gen...

journal_title：Molecular and cellular biology

authors： Singer MJ,Marcotte BA,Selker EU

更新日期：1995-10-01 00:00:00

abstract：:The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost all NuA4 subunits have clear homol...

journal_title：Molecular and cellular biology

authors： Doyon Y,Selleck W,Lane WS,Tan S,Côté J

更新日期：2004-03-01 00:00:00

abstract：:Histone H3 lysine 9 (H3K9) methylation has broad roles in transcriptional repression, gene silencing, maintenance of heterochromatin, and epigenetic inheritance of heterochromatin. Using Xenopus laevis oocytes, we have previously shown that targeting G9a, an H3K9 histone methyltransferase, to chromatin increases H3K9 ...

journal_title：Molecular and cellular biology

authors： Stewart MD,Sommerville J,Wong J

更新日期：2006-09-01 00:00:00

abstract：:Fibroblast growth factors (FGFs) induce a variety of biological effects on different cell types. They activate a number of genes, including immediate-early genes, such as the transcription factors Fos and Jun, which are also common targets for other tyrosine kinase receptor-activating growth factors. Here we describe ...

journal_title：Molecular and cellular biology

authors： Jaakkola P,Vihinen T,Määttä A,Jalkanen M

更新日期：1997-06-01 00:00:00

abstract：:The treatment of cells with histone deacetylase inhibitors (HDACi) was reported to reveal the acetylation of STAT1 at lysine 410 and lysine 413 (O. H. Krämer et al., Genes Dev. 20:473-485, 2006). STAT1 acetylation was proposed to regulate apoptosis by facilitating binding to NF-κB and to control immune responses by su...

journal_title：Molecular and cellular biology

authors： Antunes F,Marg A,Vinkemeier U

更新日期：2011-07-01 00:00:00

abstract：:Compromised immunoregulation contributes to obesity and complications in metabolic pathogenesis. Here, we demonstrate that the nuclear factor of activated T cell (NFAT) group of transcription factors contributes to glucose and insulin homeostasis. Expression of two members of the NFAT family (NFATc2 and NFATc4) is ind...

journal_title：Molecular and cellular biology

authors： Yang TT,Suk HY,Yang X,Olabisi O,Yu RY,Durand J,Jelicks LA,Kim JY,Scherer PE,Wang Y,Feng Y,Rossetti L,Graef IA,Crabtree GR,Chow CW

更新日期：2006-10-01 00:00:00

abstract：:We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct positive selection. Using conventional targeting for the first step, a functional neo gene and a nonfunctional HPRT minigene (the "socket") are introduced into the genome of HPRT- embryonic stem (ES) cells close to the chosen...

journal_title：Molecular and cellular biology

authors： Detloff PJ,Lewis J,John SW,Shehee WR,Langenbach R,Maeda N,Smithies O

更新日期：1994-10-01 00:00:00

abstract：:To overcome the difficulty of assessing oncogene action in human epithelial cell types, such as thyroid, which have limited proliferative potential in culture, we have explored the use of temperature-sensitive (ts) mutants of simian virus 40 (SV40) early region to create conditionally immortalized epithelial cell line...

journal_title：Molecular and cellular biology

authors： Wynford-Thomas D,Bond JA,Wyllie FS,Burns JS,Williams ED,Jones T,Sheer D,Lemoine NR

更新日期：1990-10-01 00:00:00

abstract：:Estrogen receptors (ERs) are hormone-regulated transcription factors that regulate key aspects of reproduction and development. ERs are unusual in that they do not typically repress transcription in the absence of hormone but instead possess otherwise cryptic repressive functions that are revealed upon binding to cert...

journal_title：Molecular and cellular biology

authors： Varlakhanova N,Snyder C,Jose S,Hahm JB,Privalsky ML

更新日期：2010-03-01 00:00:00

abstract：:Mutagenesis studies have demonstrated the requirement for the CD28-responsive element (CD28RE) within the interleukin-2 (IL-2) promoter for transcriptional upregulation by CD28. Here, we demonstrate that CD28 responsiveness is conferred by a composite element containing both the CD28RE and the NF-IL-2B AP-1 sites (RE/...

journal_title：Molecular and cellular biology

authors： Shapiro VS,Truitt KE,Imboden JB,Weiss A

更新日期：1997-07-01 00:00:00

abstract：:Previous work showed that tunicamycin suppresses glycosylation of laminin. In the present work, the role of glycosylation in the secretion of laminin and in the disulfide bonding of laminin subunits was studied, using tunicamycin to inhibit glycosylation. Tunicamycin inhibited extensively the secretion of laminin into...

journal_title：Molecular and cellular biology

authors： Howe CC

更新日期：1984-01-01 00:00:00

abstract：:We employed a protein gel blotting procedure to search for nuclear proteins from rat pituitary cells that bind preferentially to the 5'-flanking region of the rat prolactin gene. By gel blots of chromatin proteins from GH3 rat pituitary tumor cells with a 32P-labeled prolactin genomic clone, we detected two major bind...

journal_title：Molecular and cellular biology

authors： White BA,Preston GM,Lufkin TC,Bancroft C

更新日期：1985-11-01 00:00:00

abstract：:This paper presents a general means of eliminating the function of a single protein without relying on genetic alterations in its structure or level of synthesis. The strategy is based on the inducible cellular expression of neutralizing antibody to inactivate the protein selectively. The feasibility of this approach ...

journal_title：Molecular and cellular biology

authors： Carlson JR

更新日期：1988-06-01 00:00:00

abstract：:A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had ...

journal_title：Molecular and cellular biology

authors： Beck TW,Brennscheidt U,Sithanandam G,Cleveland J,Rapp UR

更新日期：1990-07-01 00:00:00

abstract：:The serum response factor (p67SRF) binds to a palindromic sequence in the c-fos serum response element (SRE). A second protein, p62TCF binds in conjunction with p67SRF to form a ternary complex, and it is through this complex that growth factor-induced transcriptional activation of c-fos is thought to take place. A 90...

journal_title：Molecular and cellular biology

authors： Sharrocks AD,Gille H,Shaw PE

更新日期：1993-01-01 00:00:00

abstract：:A series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei: (i) a major fraction (60 to 90%) which is retained in DNA- and RNA-depleted nuclei after low- and high-salt extraction, (ii) a small fraction (1%) relea...

journal_title：Molecular and cellular biology

authors： Eisenman RN,Tachibana CY,Abrams HD,Hann SR

更新日期：1985-01-01 00:00:00

abstract：:Generally, histone deacetylase (HDAC) inhibitor-induced p21(Waf1/Cip1) expression is thought to be p53 independent. Here we found that an inhibitor of HDAC, depsipeptide (FR901228), but not trichostatin A (TSA), induces p21(Waf1/Cip1) expression through both p53 and Sp1/Sp3 pathways in A549 cells (which retain wild-ty...

journal_title：Molecular and cellular biology

authors： Zhao Y,Lu S,Wu L,Chai G,Wang H,Chen Y,Sun J,Yu Y,Zhou W,Zheng Q,Wu M,Otterson GA,Zhu WG

更新日期：2006-04-01 00:00:00