Abstract:
:A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had features of a housekeeping gene in that it was GC-rich (HTF-like), lacked TATA and CAAT boxes, and contained heterogeneous RNA start sites and four potential binding sites for the transcription factor SP1. In addition, an octamer motif (ATTTCAT), a potential binding site for the octamer family of transcription factors, was located at -734 base pairs. The Raf-1 promoter region drove reporter gene expression 30-fold over the promoterless reporter in Cos 7 cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Beck TW,Brennscheidt U,Sithanandam G,Cleveland J,Rapp URdoi
10.1128/mcb.10.7.3325subject
Has Abstractpub_date
1990-07-01 00:00:00pages
3325-33issue
7eissn
0270-7306issn
1098-5549journal_volume
10pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00